CIMMYT小麦种质C615抗叶锈病QTL分析
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摘要
由Puccinia triticina引起的叶锈病是小麦主要病害之一,引进种质C615具有叶锈病成株期抗性,但其抗病性遗传机制尚不清楚。本研究以抗病亲本C615与高感叶锈病亲本宁麦18构建的F_(2:7)代重组自交系群体为材料,利用337对多态性SSR标记构建遗传连锁图谱,结合2016、2017连续两年的叶锈病鉴定结果进行复合区间作图,结果在1BL、2DS、3BS、4DL和6BS染色体上共发现了5个抗性QTL,暂命名为QLr.njau-1BL、QLr.njau-2DS、QLr.njau-3BS、QLr.njau-4DL和QLr.njau-6BS。其中,QLr.njau-1BL、QLr.njau-3BS和QLr.njau-4DL在两年均被检测到,分别解释10.1%~15.7%、10.9%~13.5%和8.2%~9.0%的表型变异;另2个QTL只在一年被检测到,解释6.2%和9.2%的表型变异。除QLr.njau-2DS外的4个抗性QTL均来源于抗病亲本C615。QLr.njau-1BL和QLr.njau-4DL分别与已报道的慢病性基因Lr46、Lr67在同一区域,QLr.njau-3B可能为一个新的抗叶锈病QTL。此外,本研究在C615/扬麦13(轮回亲本)BC4F5回交群体中选出了15个农艺性状优良且抗叶锈病的株系,利用与C615所含抗性QTL紧密连锁的7个SSR标记对其进行基因型检测,结果显示所有这15个株系均含有来自C615的抗性QTL,且有3个株系聚合了全部抗性位点,表明C615可作为抗源亲本用于高产、抗病育种。本研究结果将为分子标记选育抗叶锈品种提供材料和技术支撑。
Leaf rust, caused by Puccinia triticina, is an important wheat disease. The introduced wheat line C615 shows adult resistance to leaf rust, but its mechanism is not clear. In this study, the quantitative trait loci(QTLs) for leaf rust resistance were identified and located in a linkage map constructed with 337 SSR markers using a recombinant inbred line(RIL) population(112 lines of the F_(2:7) generation) derived from CIMMYT wheat C615(resistant) and Ningmai 18(highly susceptible). Final disease severity(FDS) of leaf rust was evaluated in 2016 and 2017. Five QTLs were detected on chromosomes 1 BL, 2 DS, 3 BS, 4 DL, and 6 BS, designated QLr.njau-1 BL, QLr.njau-2 DS, QLr.njau-3 BS, QLr.njau-4 DL, and QLr.njau-6 BS, respectively. All the resistance alleles were contributed by the resistant parent C615, except for QLr.njau-2 DS. Three QTLs, QLr.njau-1 BL, QLr.njau-3 BS, and QLr.njau-4 DL, were detected in both years, which explained phenotypic variance by 10.1%–15.7%, 10.9%–13.5%, and 8.2%–9.0%, respectively. The remaining two QTLs were detected in one year with smaller contributions(6.2% and 9.2%, respectively). QLr.njau-1 BL and QLr.njau-4 DL were located in the same regions of the known resistance genes Lr46 and Lr67, respectively. QLr.njau-3 BS in the marker interval Xbarc102–Xwmc623 is probably a novel QTL for leaf rust resistance. Fifteen BC4 F5 lines derived from the cross of C615/Yangmai 13(recurrent parent) were genotyped for leaf rust resistance using seven SSR markers closely linked to the QTLs from C615. All the 15 lines had QTLs donated by C615 and three of them pyramided four QTLs, indicating that C615 can serve as an elite donor parent in wheat breeding aiming at high-yield and high-resistance to leaf rust. These results provide a basis of marker-assisted selection and providing breeding materials.
Leaf rust, caused by Puccinia triticina, is an important wheat disease. The introduced wheat line C615 shows adult resistance to leaf rust, but its mechanism is not clear. In this study, the quantitative trait loci(QTLs) for leaf rust resistance were identified and located in a linkage map constructed with 337 SSR markers using a recombinant inbred line(RIL) population(112 lines of the F_(2:7) generation) derived from CIMMYT wheat C615(resistant) and Ningmai 18(highly susceptible). Final disease severity(FDS) of leaf rust was evaluated in 2016 and 2017. Five QTLs were detected on chromosomes 1 BL, 2 DS, 3 BS, 4 DL, and 6 BS, designated QLr.njau-1 BL, QLr.njau-2 DS, QLr.njau-3 BS, QLr.njau-4 DL, and QLr.njau-6 BS, respectively. All the resistance alleles were contributed by the resistant parent C615, except for QLr.njau-2 DS. Three QTLs, QLr.njau-1 BL, QLr.njau-3 BS, and QLr.njau-4 DL, were detected in both years, which explained phenotypic variance by 10.1%–15.7%, 10.9%–13.5%, and 8.2%–9.0%, respectively. The remaining two QTLs were detected in one year with smaller contributions(6.2% and 9.2%, respectively). QLr.njau-1 BL and QLr.njau-4 DL were located in the same regions of the known resistance genes Lr46 and Lr67, respectively. QLr.njau-3 BS in the marker interval Xbarc102–Xwmc623 is probably a novel QTL for leaf rust resistance. Fifteen BC4 F5 lines derived from the cross of C615/Yangmai 13(recurrent parent) were genotyped for leaf rust resistance using seven SSR markers closely linked to the QTLs from C615. All the 15 lines had QTLs donated by C615 and three of them pyramided four QTLs, indicating that C615 can serve as an elite donor parent in wheat breeding aiming at high-yield and high-resistance to leaf rust. These results provide a basis of marker-assisted selection and providing breeding materials.
引文
[1]Khan M H,Bukhari A,Dar Z A,Rizvi S M.Status and strategies in breeding for rust resistance in wheat.Agric Sci,2013,4:292–301
[2]Singh D,Mohler V,Park R F.Discovery,characterization and mapping of wheat leaf rust resistance gene Lr71.Euphytica,2013,190:131–136
[3]Zhang P P,Yin G H,Zhou Y,Qi A Y,Gao F M,Xia X C,He Z H,Li Z F,Liu D Q,QTL mapping of adult-plant resistance to leaf rust in the wheat cross Zhou 8425B/Chinese Spring using high-density SNP markers.Front Plant Sci,2017,8:793–805
[4]Do Vale F X R,Parlevliet J E,Zambolim L.Concepts in plant disease resistance.Trop Plant Pathol,2001,26:577–589
[5]Spielmeyer W,Mcintosh R J,Lagudah E.Powdery mildew resistance and Lr34/Yr18 genes for durable resistance to leaf and stripe rust cosegregate at a locus on the short arm of chromosome7D of wheat.Theor Appl Genet,2005,111:731–735
[6]Bariana H S,Hayden M J,Ahmed N U,Bell J A,Sharp P J,Mc Intosh R A.Mapping of durable adult plant and seedling resistances to stripe rust and stem rust diseases in wheat.Aust J Agric Res,2001,52:1247–1255
[7]Suenaga K,Singh R P,Huertaespino J,William H M.Microsatellite markers for genes Lr34/Yr18 and other quantitative trait loci for leaf rust and stripe rust resistance in bread wheat.Phytopathology,2003,93:881–890
[8]Schnurbusch T,Paillard S,Schori A,Messmer M,Schachermayr G,Winzeler M,Keller B.Dissection of quantitative and durable leaf rust resistance in Swiss winter wheat reveals a major resistance QTL in the Lr34 chromosomal region.Theor Appl Genet,2004,108:477–484
[9]Schnurbusch T,Bossolini E,Messmer M,Keller B.Tagging and validation of a major quantitative trait locus for leaf rust resistance and leaf tip necrosis in winter wheat cultivar Forno.Phytopathology,2004,94:1036–1041
[10]Zhang P P,Zhang Y Q,Han B,Gao P,Liu L,Xing L,Li Z F,Liu D Q.QTL mapping of adult-plant resistance genes to leaf rust and stripe rust in CIMMYT wheat Kingbird and PBW343.J Henan Agric Sci,2014,54:1907–1925
[11]William M,Singh R P,Huerta-Espino J,Islas S O,Hoisington D.Molecular marker mapping of leaf rust resistance gene Lr46 and its association with stripe rust resistance gene Yr29 in wheat.Phytopathology,2003,93:153–159
[12]Singh R P,Mujeebkazi A,Huertaespino J.Lr46:a gene conferring slow-rusting resistance to leaf rust in wheat.Phytopathology,1998,88:890–894
[13]Lan C,Basnet B R,Singh R P,Huerta-Espino J,Herrera-Foessel S A,Ren Y,Randhawa M S.Genetic analysis and mapping of adult plant resistance loci to leaf rust in durum wheat cultivar Bairds.Theor Appl Genet,2017,130:609–619
[14]Herrera-Foessel S A,Lagudah E S,Huertaespino J,Hayden M J,Bariana H S.New slow-rusting leaf rust and stripe rust resistance genes Lr67 and Yr46 in wheat are pleiotropic or closely linked.Theor Appl Genet,2011,122:239–249
[15]Hiebert C W,Thomas J B,Mc Callum B D,Humphreys D G,De Pauw R M,Hayden M J,Mago R,Schnippenkoetter W,Spielmeyer W.An introgression on wheat chromosome 4DL in RL6077(Thatcher*6/PI 250413)confers adult plant resistance to stripe rust and leaf rust(Lr67).Theor Appl Genet,2010,121:1083–1091
[16]Herrera-Foessel S A,Singh R P,Huerta-Espino J,Rosewarne G M,Periyannan S K,Viccars L,Calvo-Salazar V,Lan C,Lagudah E S.Lr68:a new gene conferring slow rusting resistance to leaf rust in wheat.Theor Appl Genet,2012,124:1475–1486
[17]Li Z F,Lan C X,He Z H,Singh R P,Rosewarne M R,Chen X M,Xia X C.Overview and application of QTL for adult plant resistance to leaf rust and powdery mildew in wheat.Crop Sci,2014,54:1907–1925
[18]Rosewarne G M,Singh R P,Huerta-Espino J,Rebetzke G J.Quantitative trait loci for slow-rusting resistance in wheat to leaf rust and stripe rust identified with multi-environment analysis.Theor Appl Genet,2008,116:1027–1034
[19]Crossa J,Burgue?o J,Dreisigacker S,Vargas M,Herrera-Foessel S A,Lillemo M,Singh R P,Trethowan R,Warburton M,Franco J,Reynolds M,Crouch J H,Ortiz R.Association analysis of historicalbread wheat germplasm using additive genetic covariance of relatives and population structure.Genetics,2007,177:1889–1913
[20]Ren Y,Li Z,He Z H,W Ling,Bai B,Lan C X,Wang C F,Zhou G,Zhu H Z,Xia X C.QTL mapping of adult-plant resistances to stripe rust and leaf rust in Chinese wheat cultivar Bainong 64.Theor Appl Genet,2012,125:1253–1262
[21]Zhou H X,Xia X C,He Z H,LI X,Wang C F,Li Z F,Liu D Q.Molecular mapping of leaf rust resistance gene Lr NJ97 in Chinese wheat line Neijiang 977671.Theor Appl Genet,2013,126:2141–2147
[22]彭红,吕国强,王江蓉.河南省2015年小麦主要病害发生特点及原因分析.中国植保导刊,2016,36(4):29–33Peng H,Lyu G Q,Wang J R.Analysis on the characteristics and causes of main diseases of wheat in Henan province in 2015.China Plant Prot,2016,36(4):29–33(in Chinese)
[23]Peterson R F,Campbell A B,Hannah A E.A diagrammatic scale for estimating rust intensity on leaves and stems of cereals.Can J Res,1948,26:496–500
[24]张晓祥,王玲,寿路路.一种快速提取小麦基因组DNA的改良CTAB方法.中国农学通报,2012,28(36):46–49Zhang X X,Wang L,Shou L L.A rapid modified CTAB method of extracting genomic DNA from wheat leaf..Chin Agric Sci Bull,2012,28(36):46–49(in Chinese with English abstract)
[25]张利军,李在峰,Morten L,夏先春,刘大群,杨文香,罗家传,王海燕.CIMMYT小麦品种Saar的叶锈成株抗性QTL分析.中国农业科学,2009,42:388–397Zhang L J,Li Z F,Morten L,Xia X C,Liu D Q,Yang W X,Luo J C,Wang H Y.QTL Mapping for adult-plant resistance to leaf rust in CIMMYT wheat cultivar Saar.Sci Agric Sin,2009,42:388–397(in Chinese with English abstract)
[26]Li H,Ribaut J M,Li Z,Wang J.Inclusive composite interval mapping(ICIM)for digenic epistasis of quantitative traits in biparental populations.Theor Appl Genet,2008,116:243–260
[27]William H M,Singh R P,Huertaespino J,Palacios G,Suenaga K.Characterization of genetic loci conferring adult plant resistance to leaf rust and stripe rust in spring wheat.Genome,2006,49:977–990
[28]Rosewarne G M,Singh R P,Huerta-Espino J,Herrera-Foessel S A,Forrest K L,Hayden M J,Rebetzke G J.Theor Appl Genet,2012,124:1283–1294
[29]Messmer M M,Seyfarth R,Keller M,Schachermayr G,Winzeler M,Zanetti S,Feuillet C,Keller B.Genetic analysis of durable leaf rust resistance in winter wheat.Theor Appl Genet,2000,100:419–431
[30]Singh R P,Mcintosh R A.Complementary genes for reaction to Puccinia recondita tritici in Triticum aestivum:I.Genetic and linkage studies.Genome,2011,26:723–735
[31]Calvo-Salazar,Singh R P,Huerta-Espino J,Cruz-Izquierdo S,Lobato-Ortiz,Sandoval-Islas S,Vargas-Hernandez M,German S,Silva P,Basnet B R,Lan C X,Herrera-Foessel S A.Genetic analysis of resistance to leaf rust and yellow rust in spring wheat cultivar Kenya Kongoni.Plant Dis,2015,99:1153–1159
[32]Singh R P,Huerta-Espino J,William H M.Genetics and breeding for durable resistance to leaf and stripe rusts in wheat.Turkish J Agric For,2005,29:121–127
[2]Singh D,Mohler V,Park R F.Discovery,characterization and mapping of wheat leaf rust resistance gene Lr71.Euphytica,2013,190:131–136
[3]Zhang P P,Yin G H,Zhou Y,Qi A Y,Gao F M,Xia X C,He Z H,Li Z F,Liu D Q,QTL mapping of adult-plant resistance to leaf rust in the wheat cross Zhou 8425B/Chinese Spring using high-density SNP markers.Front Plant Sci,2017,8:793–805
[4]Do Vale F X R,Parlevliet J E,Zambolim L.Concepts in plant disease resistance.Trop Plant Pathol,2001,26:577–589
[5]Spielmeyer W,Mcintosh R J,Lagudah E.Powdery mildew resistance and Lr34/Yr18 genes for durable resistance to leaf and stripe rust cosegregate at a locus on the short arm of chromosome7D of wheat.Theor Appl Genet,2005,111:731–735
[6]Bariana H S,Hayden M J,Ahmed N U,Bell J A,Sharp P J,Mc Intosh R A.Mapping of durable adult plant and seedling resistances to stripe rust and stem rust diseases in wheat.Aust J Agric Res,2001,52:1247–1255
[7]Suenaga K,Singh R P,Huertaespino J,William H M.Microsatellite markers for genes Lr34/Yr18 and other quantitative trait loci for leaf rust and stripe rust resistance in bread wheat.Phytopathology,2003,93:881–890
[8]Schnurbusch T,Paillard S,Schori A,Messmer M,Schachermayr G,Winzeler M,Keller B.Dissection of quantitative and durable leaf rust resistance in Swiss winter wheat reveals a major resistance QTL in the Lr34 chromosomal region.Theor Appl Genet,2004,108:477–484
[9]Schnurbusch T,Bossolini E,Messmer M,Keller B.Tagging and validation of a major quantitative trait locus for leaf rust resistance and leaf tip necrosis in winter wheat cultivar Forno.Phytopathology,2004,94:1036–1041
[10]Zhang P P,Zhang Y Q,Han B,Gao P,Liu L,Xing L,Li Z F,Liu D Q.QTL mapping of adult-plant resistance genes to leaf rust and stripe rust in CIMMYT wheat Kingbird and PBW343.J Henan Agric Sci,2014,54:1907–1925
[11]William M,Singh R P,Huerta-Espino J,Islas S O,Hoisington D.Molecular marker mapping of leaf rust resistance gene Lr46 and its association with stripe rust resistance gene Yr29 in wheat.Phytopathology,2003,93:153–159
[12]Singh R P,Mujeebkazi A,Huertaespino J.Lr46:a gene conferring slow-rusting resistance to leaf rust in wheat.Phytopathology,1998,88:890–894
[13]Lan C,Basnet B R,Singh R P,Huerta-Espino J,Herrera-Foessel S A,Ren Y,Randhawa M S.Genetic analysis and mapping of adult plant resistance loci to leaf rust in durum wheat cultivar Bairds.Theor Appl Genet,2017,130:609–619
[14]Herrera-Foessel S A,Lagudah E S,Huertaespino J,Hayden M J,Bariana H S.New slow-rusting leaf rust and stripe rust resistance genes Lr67 and Yr46 in wheat are pleiotropic or closely linked.Theor Appl Genet,2011,122:239–249
[15]Hiebert C W,Thomas J B,Mc Callum B D,Humphreys D G,De Pauw R M,Hayden M J,Mago R,Schnippenkoetter W,Spielmeyer W.An introgression on wheat chromosome 4DL in RL6077(Thatcher*6/PI 250413)confers adult plant resistance to stripe rust and leaf rust(Lr67).Theor Appl Genet,2010,121:1083–1091
[16]Herrera-Foessel S A,Singh R P,Huerta-Espino J,Rosewarne G M,Periyannan S K,Viccars L,Calvo-Salazar V,Lan C,Lagudah E S.Lr68:a new gene conferring slow rusting resistance to leaf rust in wheat.Theor Appl Genet,2012,124:1475–1486
[17]Li Z F,Lan C X,He Z H,Singh R P,Rosewarne M R,Chen X M,Xia X C.Overview and application of QTL for adult plant resistance to leaf rust and powdery mildew in wheat.Crop Sci,2014,54:1907–1925
[18]Rosewarne G M,Singh R P,Huerta-Espino J,Rebetzke G J.Quantitative trait loci for slow-rusting resistance in wheat to leaf rust and stripe rust identified with multi-environment analysis.Theor Appl Genet,2008,116:1027–1034
[19]Crossa J,Burgue?o J,Dreisigacker S,Vargas M,Herrera-Foessel S A,Lillemo M,Singh R P,Trethowan R,Warburton M,Franco J,Reynolds M,Crouch J H,Ortiz R.Association analysis of historicalbread wheat germplasm using additive genetic covariance of relatives and population structure.Genetics,2007,177:1889–1913
[20]Ren Y,Li Z,He Z H,W Ling,Bai B,Lan C X,Wang C F,Zhou G,Zhu H Z,Xia X C.QTL mapping of adult-plant resistances to stripe rust and leaf rust in Chinese wheat cultivar Bainong 64.Theor Appl Genet,2012,125:1253–1262
[21]Zhou H X,Xia X C,He Z H,LI X,Wang C F,Li Z F,Liu D Q.Molecular mapping of leaf rust resistance gene Lr NJ97 in Chinese wheat line Neijiang 977671.Theor Appl Genet,2013,126:2141–2147
[22]彭红,吕国强,王江蓉.河南省2015年小麦主要病害发生特点及原因分析.中国植保导刊,2016,36(4):29–33Peng H,Lyu G Q,Wang J R.Analysis on the characteristics and causes of main diseases of wheat in Henan province in 2015.China Plant Prot,2016,36(4):29–33(in Chinese)
[23]Peterson R F,Campbell A B,Hannah A E.A diagrammatic scale for estimating rust intensity on leaves and stems of cereals.Can J Res,1948,26:496–500
[24]张晓祥,王玲,寿路路.一种快速提取小麦基因组DNA的改良CTAB方法.中国农学通报,2012,28(36):46–49Zhang X X,Wang L,Shou L L.A rapid modified CTAB method of extracting genomic DNA from wheat leaf..Chin Agric Sci Bull,2012,28(36):46–49(in Chinese with English abstract)
[25]张利军,李在峰,Morten L,夏先春,刘大群,杨文香,罗家传,王海燕.CIMMYT小麦品种Saar的叶锈成株抗性QTL分析.中国农业科学,2009,42:388–397Zhang L J,Li Z F,Morten L,Xia X C,Liu D Q,Yang W X,Luo J C,Wang H Y.QTL Mapping for adult-plant resistance to leaf rust in CIMMYT wheat cultivar Saar.Sci Agric Sin,2009,42:388–397(in Chinese with English abstract)
[26]Li H,Ribaut J M,Li Z,Wang J.Inclusive composite interval mapping(ICIM)for digenic epistasis of quantitative traits in biparental populations.Theor Appl Genet,2008,116:243–260
[27]William H M,Singh R P,Huertaespino J,Palacios G,Suenaga K.Characterization of genetic loci conferring adult plant resistance to leaf rust and stripe rust in spring wheat.Genome,2006,49:977–990
[28]Rosewarne G M,Singh R P,Huerta-Espino J,Herrera-Foessel S A,Forrest K L,Hayden M J,Rebetzke G J.Theor Appl Genet,2012,124:1283–1294
[29]Messmer M M,Seyfarth R,Keller M,Schachermayr G,Winzeler M,Zanetti S,Feuillet C,Keller B.Genetic analysis of durable leaf rust resistance in winter wheat.Theor Appl Genet,2000,100:419–431
[30]Singh R P,Mcintosh R A.Complementary genes for reaction to Puccinia recondita tritici in Triticum aestivum:I.Genetic and linkage studies.Genome,2011,26:723–735
[31]Calvo-Salazar,Singh R P,Huerta-Espino J,Cruz-Izquierdo S,Lobato-Ortiz,Sandoval-Islas S,Vargas-Hernandez M,German S,Silva P,Basnet B R,Lan C X,Herrera-Foessel S A.Genetic analysis of resistance to leaf rust and yellow rust in spring wheat cultivar Kenya Kongoni.Plant Dis,2015,99:1153–1159
[32]Singh R P,Huerta-Espino J,William H M.Genetics and breeding for durable resistance to leaf and stripe rusts in wheat.Turkish J Agric For,2005,29:121–127