星天牛不同为害状态下山核桃挥发物成分的GC-EAD和行为反应
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摘要
【目的】为了挖掘对星天牛Anoplophora chinensis有行为反应的山核桃Juglans mandshurica挥发物。【方法】采用动态顶空套袋吸附法收集不同为害状态[健康(CK)、天牛取食、产卵和钻蛀后]山核桃释放的挥发物,并运用气相色谱-质谱联用仪(GC-MS)、气相色谱-触角电位测量系统(GC-EAD)及"Y"型嗅觉仪技术分析鉴定出对星天牛产生电生理及行为反应的挥发物。【结果】GC-EAD反应结果表明:星天牛成虫对不同为害状态下山核桃释放的挥发物中的蒎烯、罗勒烯、对二乙苯、萜品烯、壬醛、α-萜品醇、五甲基苯、丙烯酸-2-乙基己酯、2,6-二甲基萘以及正十六烷10种挥发物产生显著的触角电位反应,对其他挥发物无反应,其中蒎烯和罗勒烯的反应较强。嗅觉反应结果表明:蒎烯仅对星天牛雄虫产生显著的引诱作用(P≤0.05),罗勒烯对星天牛雌雄虫产生极显著的引诱作用(P≤0.01);丙烯酸-2-乙基己酯对星天牛雌虫产生显著的驱避作用(P≤0.05),对二乙苯仅对星天牛雄虫产生显极著的驱避作用(P≤0.01),壬醛对星天牛雄虫产生显著的驱避作用(P≤0.05)。【结论】本研究结果表明,罗勒烯对星天牛雌成虫产生较强的引诱作用,而丙烯酸-2-乙基己酯对其雌成虫产生较好的驱避作用;蒎烯和罗勒烯对星天牛雄成虫产生较强的引诱作用,而对二乙苯和壬醛则对其雄成虫产生较好的驱避作用。
[Objectives] To explore volatiles of Juglans mandshurica and their effect on the brown mulberry longhorn beetle,Anoplophora chinensis, in order to provide a theoretical basis for screening attractants or repellents to control this insect pest.[Methods] Volatiles released from J. mandshurica subject to different kinds of damage [undamaged(CK), and plants subject to feeding, oviposition and boring damage by longicorn beetles] were collected using dynamic headspace adsorption, and those causing electrophysiological and behavioral responses in A. chinensis were identified by gas chromatography-mass spectrometry(GC-MS), a gas chromatography-electrophysiological antennal detecting system(GC-EAD) and Y-tube olfactometer experiments. [Results] A. chinensis adults had significant electrophysiological responses to ten volatiles of J.mandshurica, including(1 R)-(+)-alpha-pinene, ocimene, p-diethylbenzene, g-terpinene, nonanal, α-terpineol, pentamethyl phenyl, 2-ethylhexyl acrylate, 2,6-dimethylnaphthalene and hexadecane, but no response to the other volatiles. Female and male adults had stronger electrophysiological responses to terpenes and ocimene. The volatile(1 R)-(+)-alpha-pinene caused a significant olfactory reaction in male adults(P < 0.05), and ocimene was extremely attractive to female and male adults(P <0.01). 2-ethylhexyl acrylate was strongly repellent to female adults(P < 0.05) and p-diethylbenzene was highly attractive to males(P < 0.01). Nonanal was repellent to male adults(P < 0.05). [Conclusion] Adult female A. chinensis are strongly attracted to ocimene and repelled by 2-ethylhexyl acrylate. Male adults are strongly attracted to(1 R)-(+)-alpha-pinene and ocimene and repelled by p-diethylbenzene and nonanal.
[Objectives] To explore volatiles of Juglans mandshurica and their effect on the brown mulberry longhorn beetle,Anoplophora chinensis, in order to provide a theoretical basis for screening attractants or repellents to control this insect pest.[Methods] Volatiles released from J. mandshurica subject to different kinds of damage [undamaged(CK), and plants subject to feeding, oviposition and boring damage by longicorn beetles] were collected using dynamic headspace adsorption, and those causing electrophysiological and behavioral responses in A. chinensis were identified by gas chromatography-mass spectrometry(GC-MS), a gas chromatography-electrophysiological antennal detecting system(GC-EAD) and Y-tube olfactometer experiments. [Results] A. chinensis adults had significant electrophysiological responses to ten volatiles of J.mandshurica, including(1 R)-(+)-alpha-pinene, ocimene, p-diethylbenzene, g-terpinene, nonanal, α-terpineol, pentamethyl phenyl, 2-ethylhexyl acrylate, 2,6-dimethylnaphthalene and hexadecane, but no response to the other volatiles. Female and male adults had stronger electrophysiological responses to terpenes and ocimene. The volatile(1 R)-(+)-alpha-pinene caused a significant olfactory reaction in male adults(P < 0.05), and ocimene was extremely attractive to female and male adults(P <0.01). 2-ethylhexyl acrylate was strongly repellent to female adults(P < 0.05) and p-diethylbenzene was highly attractive to males(P < 0.01). Nonanal was repellent to male adults(P < 0.05). [Conclusion] Adult female A. chinensis are strongly attracted to ocimene and repelled by 2-ethylhexyl acrylate. Male adults are strongly attracted to(1 R)-(+)-alpha-pinene and ocimene and repelled by p-diethylbenzene and nonanal.
引文
Allison JD,Borden JH,Seybold SJ,2004.A review of the chemical ecology of the Cerambycidae(Coleoptera).Chemoecology,14(3/4):123-150.
Allison JD,Carde RT,2008.Male pheromone blend preference function measured in choice and no-choice wind tunnel trials with almond moths,Cadra cautella.Anim.Behav.,75(1):259-266.
Barata EN,Mustaparta H,Pickentt JA,Wadhams LJ,Araujo J,2002.Encoding of host and non-host plant odours by receptor neurons in the eucalyptus woodborer,Phoracantha semipunctata(Coleoptera:Cerambycidae).J.Comp.Physiol.,188(2):121-133.
Bertschy C,Turlings TCJ,Bellotti AC,Dorn AS,1997.Chemically-mediated attraction of three parasitoid species to mealybug-infested cassavaleaves.Fla.Entomol.,80(3):383-395.
Bi YG,Guo L,Wang ZG,Lv F,Yan AH,2017.Electroantennogram and behavioral responses of Apriona germari to five plant volatiles and their mixtures.Sci.Sericult.,43(1):32-38.[毕拥国,郭丽,王志刚,吕飞,阎爱华,2017.桑天牛对5种植物源挥发性化合物及其混合物的触角电位和行为反应.蚕业科学,43(1):32-38.]
Gaag DJ,Sinatra G,Roversi PF,Loomans A,Hérard F,Vukadin A,2010.Evaluation of eradication measures against Anoplophora chinensis in early stage infestations in Europe.Eppo Bulletin,40(2):176-187.
Hern A,Dorn S,2004.A female-specific attractant for the codling moth,Cydia pomonella,from apple fruit volatiles.Naturwissenschaften,91(2):77-80.
Li JQ,Fan H,Jin YJ,2003.Behavior response of Anoplophora glabripennis(Motsh.)to the mechanical-wounded and herbivorefed Acer negundo L.J.Beijing For.Univ.,25(5):42-46.[李继泉,樊慧,金幼菊,2003.光肩星天牛对损伤后复叶槭植株的行为反应.北京林业大学学报,25(5):42-46.]
Liu JM,2013.Stusies on the role Anoplophora chinensis(F.)by alluring adult with Melia azedarach L.Master dissertation.Lin’an:Zhejiang A&F University.[刘佳敏,2013.苦楝对星天牛的引诱作用研究.硕士学位论文,临安:浙江农林大学.]
Luo YQ,Huang JF,Li JG,2000.Central achievements problems andprospects on poplar longhorned beetle in China.Entomological Knowledge,37(2):116-122.[骆有庆,黄竞芳,李建光,2000,我国杨树天牛研究的主要成就、问题及展望.昆虫知识,37(2):116-122.]
Luo YQ,Liu RG,Xu ZC,Sun CC,Wen JB,2002.Theories andtechnologies of ecologically regulating poplar longhorned beetle disaster in shelter forest.J.Beijing For.Univ.,24(6):160-164.[骆有庆,刘荣光,许志春,孙长春,温俊宝,2002.防护林杨树天牛灾害的生态调控理论与技术.北京林业大学学报,246):160-164.]
MacLeod A,Evans HF,Baker RHA,2002.An analysis of pest risk froman Asian longhorn beetle(Anoplophora glabripennis)to hardwoodtrees in the European community.Crop Prot.,21(8):635-645.
Müller C,Hilker M,2000.The effect of a green leaf volatile on host plant finding by larvae of herbivorous insect.Naturwissenschafen,87(5):216-219.
Ngumbi E,Chen L,Fadamiro HY,2009.Comparative GC-EADResponses of a specialist(Microplitis croceipes)and a generalist(Cotesia marginiventris)parasitoid to cotton volatiles induced by two caterpillar species.J.Chem.Ecol.,35(9):1009-1020.
ParéPW,Tumlinson JH,1999.Plant volatiles as a defense against insect herbivores.Plant Physiol.,121(2):325-331.
Pichersky E,Gershenzon J,2002.The formation and function of plant volatiles:perfumes for pollinator attraction and defense.Curr.Opin.Plant Biol.,5(3):237-243.
Tooker JH,Crumrin AL,Hanks LM,2005.Plant volatiles are behavioral cues for adult females of the gall wasp Antistrophus rufus.Chemoecology,15(2):85-88.
Wang BX,Yang H,Yang W,Yang CP,Cai Y,Zhou M,He XH,2014.EAG and behavioral responses of Batocera lineolata Chevrolat(Coleoptera:Cerambycidae)to ten plant volatiles.Chinese Journal of Applied Entomology,51(2):481-489.[王保新,杨桦,杨伟,杨春平,蔡艳,周梦,何晓华,2014.云斑天牛对10种植物挥发物的EAG和行为反应.应用昆虫学报,51(2):481-489.]
Wang HW,Yang W,Yang H,Yang CP,Zhu XQ,Liu FJ,Jiang LH,Shan CY,2014.Electroantennogram and behavioral responses of Tomicus minor(Hartig)(Coleoptera:Scolytidae)to plant volatiles.Chin.J.Ecol.,33(5):1260-1266.[王红伟,杨伟,杨桦,杨春平,朱晓庆,刘峰建,蒋丽华,山春燕,2014.横坑切梢小蠹对植物挥发物的电生理和行为反应.生态学杂志,33(5):1260-1266.]
Wei JR,Zhao WX,Zhang YA,2011.Research progress on Anoplophora chinensis.Plant Quarantine,25(5):81-85.[魏建荣,赵文霞,张永安,2011.星天牛研究进展.植物检疫,25(5):81-85.]
Williams L,Rodriguez-Saona C,Castle SC,Zhu S,2008.EAG-active herbivore-induced plant volatiles modify behavioral responses and host attack by an egg parasitoid.J.Chem.Ecol.,34(9):1190-1201.
Xiao GR,1992.Forest Insects of China.2nd ed.Beijing:China Forestry Press.454-455.[萧刚柔,1992.中国森林昆虫(第2版).北京:中国林业出版社.454-455.]
Yan SC,Cheng H,Yang H,Yuan HE,Zhang J,Chi DF,2003.Effects of plant volatiles on the EAG response and behavior of the grey tiger longicorn,Xylotrechus rusticus(L.)(Coleoptera:Cerambycidae).Acta Entomologica Sinica,14(2):310-313.[严善春,程红,杨慧,袁红娥,张健,迟德福,2006.青杨脊虎天牛对植物源挥发物的EAG和行为反应.昆虫学报,49(5):759-767.]
Yang H,Yang W,Liang XY,Yang MF,Yang CP,Zhu TH,Wu XL,2011.The EAG and behavioral responses of Batocera horsfieldi(Coleoptera:Cerambycidae)to the composition of volatiles.J.Kansas Entomol.Soc.,84(3):217-231.
Yang H,Yang W,Yang MF,Yang CP,Zhu TH,Huang Q,2010.Effects of plant volatiles on the EAG and behavioral responses of Batocera horsfieldi Hope(Coleopter:Cerambycidae).J.Agric.Urban Entomol.,27(1):20-32.
Zhang DY,Liu JD,Wang JY,Zhu N,Xu HC,Fan JT,2016.Two plant compound lures for trapping Batocera horsfieldi(Hope)in the field.Chinese Journal Applied Entomology,53(4):856-863.[张冬勇,柳建定,王菊英,朱宁,徐华潮,樊建庭,2016.两种对云斑天牛有林间诱捕效果的植物源物质.应用昆虫学报,53(4):856-863.]
Zhu N,Zhang DY,Wu LP,Hu Q,Fan JT,2017.Attractiveness of aggregation pheromones and host plant volatiles to Anoplophora glabripennis and A.chinensis(Coleoptera:Cerambycidae).Acta Entomol.Sin.,60(4):421-430.[朱宁,张冬勇,吴利平,胡琴,樊建庭,2017.聚集信息素和寄主植物挥发物对光肩星天牛和星天牛的引诱作用.昆虫学报,60(4):421-430.]
Allison JD,Carde RT,2008.Male pheromone blend preference function measured in choice and no-choice wind tunnel trials with almond moths,Cadra cautella.Anim.Behav.,75(1):259-266.
Barata EN,Mustaparta H,Pickentt JA,Wadhams LJ,Araujo J,2002.Encoding of host and non-host plant odours by receptor neurons in the eucalyptus woodborer,Phoracantha semipunctata(Coleoptera:Cerambycidae).J.Comp.Physiol.,188(2):121-133.
Bertschy C,Turlings TCJ,Bellotti AC,Dorn AS,1997.Chemically-mediated attraction of three parasitoid species to mealybug-infested cassavaleaves.Fla.Entomol.,80(3):383-395.
Bi YG,Guo L,Wang ZG,Lv F,Yan AH,2017.Electroantennogram and behavioral responses of Apriona germari to five plant volatiles and their mixtures.Sci.Sericult.,43(1):32-38.[毕拥国,郭丽,王志刚,吕飞,阎爱华,2017.桑天牛对5种植物源挥发性化合物及其混合物的触角电位和行为反应.蚕业科学,43(1):32-38.]
Gaag DJ,Sinatra G,Roversi PF,Loomans A,Hérard F,Vukadin A,2010.Evaluation of eradication measures against Anoplophora chinensis in early stage infestations in Europe.Eppo Bulletin,40(2):176-187.
Hern A,Dorn S,2004.A female-specific attractant for the codling moth,Cydia pomonella,from apple fruit volatiles.Naturwissenschaften,91(2):77-80.
Li JQ,Fan H,Jin YJ,2003.Behavior response of Anoplophora glabripennis(Motsh.)to the mechanical-wounded and herbivorefed Acer negundo L.J.Beijing For.Univ.,25(5):42-46.[李继泉,樊慧,金幼菊,2003.光肩星天牛对损伤后复叶槭植株的行为反应.北京林业大学学报,25(5):42-46.]
Liu JM,2013.Stusies on the role Anoplophora chinensis(F.)by alluring adult with Melia azedarach L.Master dissertation.Lin’an:Zhejiang A&F University.[刘佳敏,2013.苦楝对星天牛的引诱作用研究.硕士学位论文,临安:浙江农林大学.]
Luo YQ,Huang JF,Li JG,2000.Central achievements problems andprospects on poplar longhorned beetle in China.Entomological Knowledge,37(2):116-122.[骆有庆,黄竞芳,李建光,2000,我国杨树天牛研究的主要成就、问题及展望.昆虫知识,37(2):116-122.]
Luo YQ,Liu RG,Xu ZC,Sun CC,Wen JB,2002.Theories andtechnologies of ecologically regulating poplar longhorned beetle disaster in shelter forest.J.Beijing For.Univ.,24(6):160-164.[骆有庆,刘荣光,许志春,孙长春,温俊宝,2002.防护林杨树天牛灾害的生态调控理论与技术.北京林业大学学报,246):160-164.]
MacLeod A,Evans HF,Baker RHA,2002.An analysis of pest risk froman Asian longhorn beetle(Anoplophora glabripennis)to hardwoodtrees in the European community.Crop Prot.,21(8):635-645.
Müller C,Hilker M,2000.The effect of a green leaf volatile on host plant finding by larvae of herbivorous insect.Naturwissenschafen,87(5):216-219.
Ngumbi E,Chen L,Fadamiro HY,2009.Comparative GC-EADResponses of a specialist(Microplitis croceipes)and a generalist(Cotesia marginiventris)parasitoid to cotton volatiles induced by two caterpillar species.J.Chem.Ecol.,35(9):1009-1020.
ParéPW,Tumlinson JH,1999.Plant volatiles as a defense against insect herbivores.Plant Physiol.,121(2):325-331.
Pichersky E,Gershenzon J,2002.The formation and function of plant volatiles:perfumes for pollinator attraction and defense.Curr.Opin.Plant Biol.,5(3):237-243.
Tooker JH,Crumrin AL,Hanks LM,2005.Plant volatiles are behavioral cues for adult females of the gall wasp Antistrophus rufus.Chemoecology,15(2):85-88.
Wang BX,Yang H,Yang W,Yang CP,Cai Y,Zhou M,He XH,2014.EAG and behavioral responses of Batocera lineolata Chevrolat(Coleoptera:Cerambycidae)to ten plant volatiles.Chinese Journal of Applied Entomology,51(2):481-489.[王保新,杨桦,杨伟,杨春平,蔡艳,周梦,何晓华,2014.云斑天牛对10种植物挥发物的EAG和行为反应.应用昆虫学报,51(2):481-489.]
Wang HW,Yang W,Yang H,Yang CP,Zhu XQ,Liu FJ,Jiang LH,Shan CY,2014.Electroantennogram and behavioral responses of Tomicus minor(Hartig)(Coleoptera:Scolytidae)to plant volatiles.Chin.J.Ecol.,33(5):1260-1266.[王红伟,杨伟,杨桦,杨春平,朱晓庆,刘峰建,蒋丽华,山春燕,2014.横坑切梢小蠹对植物挥发物的电生理和行为反应.生态学杂志,33(5):1260-1266.]
Wei JR,Zhao WX,Zhang YA,2011.Research progress on Anoplophora chinensis.Plant Quarantine,25(5):81-85.[魏建荣,赵文霞,张永安,2011.星天牛研究进展.植物检疫,25(5):81-85.]
Williams L,Rodriguez-Saona C,Castle SC,Zhu S,2008.EAG-active herbivore-induced plant volatiles modify behavioral responses and host attack by an egg parasitoid.J.Chem.Ecol.,34(9):1190-1201.
Xiao GR,1992.Forest Insects of China.2nd ed.Beijing:China Forestry Press.454-455.[萧刚柔,1992.中国森林昆虫(第2版).北京:中国林业出版社.454-455.]
Yan SC,Cheng H,Yang H,Yuan HE,Zhang J,Chi DF,2003.Effects of plant volatiles on the EAG response and behavior of the grey tiger longicorn,Xylotrechus rusticus(L.)(Coleoptera:Cerambycidae).Acta Entomologica Sinica,14(2):310-313.[严善春,程红,杨慧,袁红娥,张健,迟德福,2006.青杨脊虎天牛对植物源挥发物的EAG和行为反应.昆虫学报,49(5):759-767.]
Yang H,Yang W,Liang XY,Yang MF,Yang CP,Zhu TH,Wu XL,2011.The EAG and behavioral responses of Batocera horsfieldi(Coleoptera:Cerambycidae)to the composition of volatiles.J.Kansas Entomol.Soc.,84(3):217-231.
Yang H,Yang W,Yang MF,Yang CP,Zhu TH,Huang Q,2010.Effects of plant volatiles on the EAG and behavioral responses of Batocera horsfieldi Hope(Coleopter:Cerambycidae).J.Agric.Urban Entomol.,27(1):20-32.
Zhang DY,Liu JD,Wang JY,Zhu N,Xu HC,Fan JT,2016.Two plant compound lures for trapping Batocera horsfieldi(Hope)in the field.Chinese Journal Applied Entomology,53(4):856-863.[张冬勇,柳建定,王菊英,朱宁,徐华潮,樊建庭,2016.两种对云斑天牛有林间诱捕效果的植物源物质.应用昆虫学报,53(4):856-863.]
Zhu N,Zhang DY,Wu LP,Hu Q,Fan JT,2017.Attractiveness of aggregation pheromones and host plant volatiles to Anoplophora glabripennis and A.chinensis(Coleoptera:Cerambycidae).Acta Entomol.Sin.,60(4):421-430.[朱宁,张冬勇,吴利平,胡琴,樊建庭,2017.聚集信息素和寄主植物挥发物对光肩星天牛和星天牛的引诱作用.昆虫学报,60(4):421-430.]