长柄扁桃不同提取物降血脂作用有效部位的筛选
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摘要
研究长柄扁桃药材石油醚部位、乙酸乙酯部位、正丁醇部位和水部位提取物对高脂血症大鼠血脂水平的影响,筛选其降血脂有效部位。取Wistar大鼠70只,分为正常对照组、高脂模型组、血脂康阳性药组、石油醚组、乙酸乙酯组、正丁醇组和水共7组。以高脂饲料喂养建立高脂血症大鼠模型,给予长柄扁桃4个提取部位干预5周后,观测大鼠体重和血清中总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白(low density lipoprotein cholesterol,LDL-C),丙二醛(malondialdehyde,MDA)和超氧化物歧化酶(superoxide dismutase,SOD)的含量以及谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)和碱性磷酸酶(alkaline phosphatase,ALP)的活性,计算肝脏系数、动脉硬化指数(arteriosclerosis index,AI)、HDL-C/TC和SOD/MDA比值。研究表明长柄扁桃正丁醇部位能显著降低血清TC、TG、LDL-C水平、MDA的含量、ALT、AST和ALP水平、肝系数及AI值(p<0.05),能显著提高SOD活性、SOD/MDA和HDL-C/TC比值(p>0.05);长柄扁桃石油醚部位、乙酸乙酯部位和水部位组血清TC、TG、LDL-C含量、MDA的含量、ALT和AST水平均有降低趋势,但无显著性差异(p>0.05),SOD/MDA和HDL-C/TC比值均有升高趋势,但无显著性差异(p>0.05),其中长柄扁桃石油醚提取物能显著提高SOD活性(p<0.05)。结果表明正丁醇部位具有一定降血脂作用,对肝脏有一定的保护作用,长柄扁桃正丁醇部位为优选有效部位。
The effects of Amygdalus pedunculata Pall petroleum ether,ethyl acetate,n-butanol and water extracts on serum lipid level of hyperlipidemia rats were studied,and the effective parts of lowering blood lipid were screened. 70 Wistar rats were divided into 7 groups:normal control group,high-fat model group,xuezhikang positive drug group,petroleum ether group,ethyl acetate group,n-butanol group and water group.With high fat feed hyperlipidemia rat model was established,giving Amygdalus pedunculata Pall extract four parts intervention after five weeks,observing the rat body weight and serum total cholesterol(TC),triglyceride(TG),high-density lipoprotein(HDL-C),low-density lipoprotein(LDL-C),superoxide dismutase(SOD)and model driven architecture(MDA)content and alanine aminotransferase(ALT),aspertate aminotransferase(AST)and alkaline phosphatase(ALP)activity and liver coefficient calculation,arteriosclerosis index(AI),HDL-C/TC and ratio of SOD/MDA.The n-butanol position of Amygdalus pedunculata Pall significantly reduced serum TC,TG,LdL-C level,AI value,HDL-C/TC ratio,liver coefficient,MDA content,ALT,AST and ALP levels(p<0.05),and significantly improve SOD activity and SOD/MDA ratio(p<0.05).The part of n-butanol had a certain effect of lowering blood lipids and protecting liver.
The effects of Amygdalus pedunculata Pall petroleum ether,ethyl acetate,n-butanol and water extracts on serum lipid level of hyperlipidemia rats were studied,and the effective parts of lowering blood lipid were screened. 70 Wistar rats were divided into 7 groups:normal control group,high-fat model group,xuezhikang positive drug group,petroleum ether group,ethyl acetate group,n-butanol group and water group.With high fat feed hyperlipidemia rat model was established,giving Amygdalus pedunculata Pall extract four parts intervention after five weeks,observing the rat body weight and serum total cholesterol(TC),triglyceride(TG),high-density lipoprotein(HDL-C),low-density lipoprotein(LDL-C),superoxide dismutase(SOD)and model driven architecture(MDA)content and alanine aminotransferase(ALT),aspertate aminotransferase(AST)and alkaline phosphatase(ALP)activity and liver coefficient calculation,arteriosclerosis index(AI),HDL-C/TC and ratio of SOD/MDA.The n-butanol position of Amygdalus pedunculata Pall significantly reduced serum TC,TG,LdL-C level,AI value,HDL-C/TC ratio,liver coefficient,MDA content,ALT,AST and ALP levels(p<0.05),and significantly improve SOD activity and SOD/MDA ratio(p<0.05).The part of n-butanol had a certain effect of lowering blood lipids and protecting liver.
引文
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[19]张燕菊,综述,郭莉琼,等.血清碱性磷酸酶及其与卒中的相关性进展[J].临床与病理杂志,2018,38(8):1780-1784
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[2]李霜枫,刘钰涵,陈旭.中药治疗高脂血症研究[J].辽宁中医药大学学报,2013,15(6):138-139
[3]张选明,叶丹,张虹霞,等.参苓白术散加味治疗高脂血症68例[J].中国实验方剂学杂志,2015,21(12):158-161
[4]冯利民,李立凤,杜武勋,等.高脂血症基本病机与证型规律研究进展[J].时珍国医国药,2012,23(12):3101-3103
[5]苏贵兴,姚玉卿.我国的野生扁桃资源[J].野生植物研究,1983,4(2):7-11
[6]郭春会,罗梦,马玉华,等.沙地濒危植物长柄扁桃特性研究进展[J].西北农林科技大学学报(自然科学版),2005,33(12):125-129
[7]元艺兰.郁李仁的药理作用与临床应用[J].现代医药卫生,2007,23(13):1987-1988
[8]朱序榆,王怀彪.长柄扁桃发展现状探讨[J].榆林科技,2012(3):62-64
[9]王燕,魏蔚,董发昕,等.长柄扁桃仁的营养成分分析[J],西北大学学报(自然科学版),2009,39(1):59-62
[10]候国峰,李聪,陈邦,等.不同产地长柄扁桃种仁成分分析[J].西北植物学报,2014,34(9):1843-1848
[11]白迎春,石松利,郑倩男,等.长柄扁桃水煎液对高脂血症大鼠血脂的影响[J].北方药学,2016,13(1):102-103
[12]白迎春,石松利,吴培赛,等.长柄扁桃种仁水煎液对高脂血症大鼠血脂、脂质过氧化和肝功能的影响[J].中国卫生检验杂志,2017,27(1):30-33
[13]范丽娟,李仲.LDL受体介导的血浆低密度脂蛋白胆固醇的内吞[J].生命的化学,2014, 34(3):329-336
[14]叶振南,李楠,盛丹丹,等.青钱柳多糖对高脂血症大鼠血脂及抗脂质过氧化作用的影响[J].现代食品科技,2014,30(4):1-5,20
[15]赵广高,苏全生,仇乃民,等.高温环境下大鼠一次性有氧运动后血IL-2、SOD、MDA、SOD/MDA指标的变化研究[J].成都体育学院学报,2011,37(4):86-90
[16]柳海艳,钟赣生,刘佳,等.接近临床剂量的甘遂半夏汤加减甘遂甘草反药组合对腹水大鼠肝肾功能的影响[J].北京中医药大学学报,2014,37(1):33-38
[17]张丹丹,但汉熊,黄慧辉,等.火麻仁油对高脂血症大鼠血脂代谢及保肝作用研究[J].中国药师,2015,18(4):571-597
[18]彭敏,顾施健,姜淋洁.等.白术提取物降血脂作用的有效部位研究[J].时珍国医国药,2011,22(10):2363-2365
[19]张燕菊,综述,郭莉琼,等.血清碱性磷酸酶及其与卒中的相关性进展[J].临床与病理杂志,2018,38(8):1780-1784
[20] Goldstein J L,Brown M S.Molecular medicine:The cholesterol quartet[J]. Science,2001,292(5520):1310-1312
[21] Whitney E J, Krasuski R A, Personius B E, et al. A randomized trial of a strategy for increasing high-density lipoprotein cholesterol levels:effects on progression of coronary heart disease and clinical events[J]. Annals of Internal Medicine, 2005, 14(2):95-104