脑微透析技术在神经精神疾病中的研究进展
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摘要
脑微透析是一种可对采集样品进行连续、在线、实时定量分析的技术,近年来在脑内神经递质的检测中应用广泛,在神经精神疾病的研究中有广阔的发展前景,但因其操作复杂、价格昂贵、耗时长、个体差异大、探针重现性不良等而受到限制。进一步优化实验设计、发展多位点微透析是下一步的方向,神经精神疾病脑内微环境代谢状态变化的监测是微透析技术用于此的另一前景。文章就脑微透析技术的原理、特点及操作要点,神经递质与神经精神疾病之间的关系,以及脑微透析在神经精神疾病中的研究进展进行综述。
Brain microdialysis(BMD) is a technique for continuous,on-line and real-time quantitative analysis for collected samples and it has been widely used in the quantitative detection of neurotransmitters in the brain in recent years. BMD has broad prospects in the research of neuropsychiatric diseases,but it is limited in studies because of its complicated operation,high cost,long time consuming,individual difference and poor reproducibility of the probe. Optimizing experimental design and developing multilocus microdialysis are main directions of research. Monitoring the changes of the metabolic state of the brain microenvironment in the neuropsychiatric diseases is another prospect of BMD. This article reviews the principle,characteristics and key points of brain microdialysis,along with the relationship between neurotransmitters and neuropsychiatric diseases,and the progress of microdialysis in neuropsychiatric diseases.
Brain microdialysis(BMD) is a technique for continuous,on-line and real-time quantitative analysis for collected samples and it has been widely used in the quantitative detection of neurotransmitters in the brain in recent years. BMD has broad prospects in the research of neuropsychiatric diseases,but it is limited in studies because of its complicated operation,high cost,long time consuming,individual difference and poor reproducibility of the probe. Optimizing experimental design and developing multilocus microdialysis are main directions of research. Monitoring the changes of the metabolic state of the brain microenvironment in the neuropsychiatric diseases is another prospect of BMD. This article reviews the principle,characteristics and key points of brain microdialysis,along with the relationship between neurotransmitters and neuropsychiatric diseases,and the progress of microdialysis in neuropsychiatric diseases.
引文
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[19]Oxenkrug G.Serotonin-kynurenine hypothesis of depression:historical overview and recent developments[J].Curr Drug Targets,2013,14(5):514-521.
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[25]Fujimoto Y,Funao T,Suehiro K,et al.Brain serotonin content regulates the manifestation of tramadol-induced seizures in rats:disparity between tramadol-induced seizure and serotonin syndrome[J].Anesthesiology,2015,122(1):178-189.
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[27]Granger AJ,Mulder N,Saunders A,et al.Cotransmission of acetylcholine and GABA[J].Neuropharmacology,2016,100:40-46.
[28]Traini E,Bramanti V,Amenta F.Choline alphoscerate(alphaglyceryl-phosphoryl-choline)an old choline-containing phospholipid with a still interesting profile as cognition enhancing agent[J].Curr Alzheimer Res,2013,10(10):1070-1079.
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[32]M9hler H.Role of GABAAreceptors in cognition[J].Biochem Soc Trans,2009,37(Pt 6):1328-1333.
[33]赵庆杰,齐丹,孟珊珊,等.γ-羟基丁酸对脑缺血再灌注后海马区γ-氨基丁酸及其A型受体α1亚型的影响[J].中国组织工程研究,2005,9(45):37-39.
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[35]Brown RE,Basheer R,Mc Kenna JT,et al.Control of sleep and wakefulness[J].Physiol Rev,2012,92(3):1087-1097.
[36]Coyle JT,Puttfarcken P.Oxidative stress,glutamate,and neurodegenerative disorders[J].Science,1993,262(5134):689-695.
[37]王琦,隋海娟,屈文慧,等.知母皂苷元对谷氨酸引起的皮层神经元损伤的保护作用研究[J].中国药理学通报,2013,29(2):281-285.
[38]Orset C,Parrot S,Sauvinet V,et al.Dopamine transporters are involved in the onset of hypoxia-induced dopamine efflux in striatum as revealed by in vivo microdialysis[J].Neurochem Int,2005,46(8):623-633.
[39]Ratajczak P,Kus K,Go?embiowska K,et al.The influence of aripiprazole and olanzapine on neurotransmitters level in frontal cortex of prenatally stressed rats[J].Environ Toxicol Pharmacol,2016,46:122-130.
[40]刘洋,李敏,侯悦,等.高效液相色谱-电化学法测定大鼠脑微透析液中多巴胺的方法建立及应用[J].药物分析杂志,2013,33(2):255-262.
[41]Nesbitt KM,Varner EL,Andrea JG,et al.Microdialysis in the rat striatum:effects of 24 h dexamethasone retrodialysis on evoked dopamine release and penetration injury[J].ACS Chem Neurosci,2015,6(1):163-173.
[42]Van SJ,Lens C,Maes K,et al.Reassessment of the antioxidative mixture for the challenging electrochemical determination of dopamine,noradrenaline and serotonin in microdialysis samples[J].J Chromatogr B Analyt Technol Biomed Life Sci,2015,998-999:63-71.
[43]Kamińska K,Goembiowska K,Rogóz6)Z.Effect of risperidone on the fluoxetine-induced changes in extracellular dopamine,serotonin and noradrenaline in the rat frontal cortex[J].Pharmacol Rep,2013,65(5):1144-1151.
[44]庄宗,赵旭东,史继新.微透析技术在神经外科中的应用[J].医学研究生学报,2010,23(8):880-883.
[45]Borland LM,Shi G,Yang H,et al.Voltammetric study of extracellular dopamine near microdialysis probes acutely implanted in the striatum of the anesthetized rat[J].J Neurosci Methods,2005,146(2):149-158.
[46]He Y,Zhao XE,Zhu S,et al.In situ derivatization-ultrasoundassisted dispersive liquid-liquid microextraction for the determination of neurotransmitters in Parkinson's rat brain microdialysates by ultra high performance liquid chromatography-tandem mass spectrometry[J].J Chromatogr A,2016(1458):70-81.
[47]Uutela P,ReinilR,Piepponen P,et al.Analysis of acetylcholine and choline in microdialysis samples by liquid chromatography/tandem mass spectrometry[J].Rapid Commun Mass Spectrom,2005,19(20):2950-2956.
[48]吕允凤,胡欣,毕开顺.应用微透析技术和质谱法测定川芎嗪对大鼠脑内乙酰胆碱释放量的影响[J].药学学报,2008,43(11):1128-1133.
[49]程发峰,宋文婷,邬金洋,等.精制清开灵注射液对脑缺血后大鼠脑微透析液中谷氨酸含量的影响[J].时珍国医国药,2015,26(3):582-584.
[50]Persike M,Zimmermann M,Klein J,et al.Quantitative determination of acetylcholine and choline in microdialysis samples by MALDI-TOF MS[J].Anal Chem,2010,82(3):922-929.
[51]郑芳昊,罗佳波.微透析采样考察麻黄-桂枝药对配伍对大鼠额叶皮层部位神经递质Glu和GABA水平的影响[J].时珍国医国药,2016,27(10):2335-2338.
[52]Jamal M,Ameno K,Wang W,et al.Inhibition of acetaldehyde metabolism decreases acetylcholine release in medial frontal cortex of freely moving rats[J].Brain Res,2005,1039(1-2):90-96.
[53]姚明江,张鹏,宋文婷,等.通络清脑注射液对脑缺血再灌注损伤大鼠脑微透析液中两种氨基酸水平的影响[J].中国实验方剂学杂志,2014,20(12):136-140.
[54]张春颖,杜贵友,王巍,等.微透析技术在脑缺血动物神经递质研究中的[J].中国药理学通报,2004,20(11):1209-1211.
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[2]柳琳,张相宜,肖瑶,等.脑微透析技术在胶质瘤研究中的应用进展[J].药学学报,2014,49(4):450-456.
[3]邱瑞琪,孙艳冬,张然然,等.多位点微透析取样技术在药代动力学研究中的应用进展[J].中国医药工业杂志,2016,47(10):1316-1321.
[4]Zhuang L,Xia H,Gu Y,et al.Theory and Application of Microdialysis in Pharmacokinetic Studies[J].Curr Drug Metab,2015,16(10):919-931.
[5]唐丹,肖继,陈泉,等.多巴胺及其受体免疫调节作用的研究进展[J].基础医学与临床,2016,36(4):537-541.
[6]秦幸娜,李新旺,田琳,等.多巴胺对动物冲动性的影响[J].心理科学进展,2015,23(2):241-251.
[7]Salamone JD,Pardo M,Yohn SE,et al.Mesolimbic Dopamine and the Regulation of Motivated Behavior[J].Curr Top Behav Neurosci,2016,27:231-257.
[8]Howes O,Mccutcheon R,Stone J.Glutamate and dopamine in schizophrenia:an update for the 21st century[J].J Psychopharmacol,2015,29(2):97-115.
[9]Swanson JM,Kinsbourne M,Nigg J,et al.Etiologic subtypes of attention-deficit/hyperactivity disorder:brain imaging,molecular genetic and environmental factors and the dopamin hypothesis[J].Neuropsychol Rev,2007,17(1):39-59.
[10]Hansen FH,Skjrringe T,Yasmeen S,et al.Missense dopamine transporter mutations associate with adult parkinsonism and ADHD[J].J Clin Invest,2014,124(7):3107-3120.
[11]Ossowska G,Nowa G,Kata R,et al.Brainmonoaminereceptors in achronic unpredictable stress model in rats[J].J Neural Transm,2001,108:311-319.
[12]Strobel C,Hunt S,Sullivan R,et al.Emotional regulation of pain:the role of noradrenaline in the amygdala[J].Sci China Life Sci,2014,57(4):384-390.
[13]Mc Gaugh JL,Mcintyre CK,Power AE.Amygdala modulation of memory consolidation:interaction with other brain systems[J].Neurobiol Learn Mem,2002,78(3):539-552.
[14]Luo Y,Zhou J,Li MX,et al.Reversal of aging-related emotional memory deficits by norepinephrine via regulating the stability of surface AMPA receptors[J].Aging Cell,2015,14(2):170-179.
[15]Schildkraut JJ.The echolamine hypothesis of affective disorders:a review of supporting evidence[J].Am J Psychiatry,1965,122:509-512.
[16]Ramos BP,Arnsten AF.Adrenergic pharmacology and cognition:Focus on the prefrontal cortex[J].Pharmacol Ther,2007,113(3):523-536.
[17]Bruno KJ,Hess EJ.The alpha(2c)-adrenergic receptor mediates hyperactivity of coloboma mice,a model of attention deficit hyperactivity disorder[J].Neurobiol Dis,2006,23(3):679-688.
[18]周航宇,姜淮芜,王明佳.5-羟色胺及其受体研究进展[J].山东医药,2014,54(12):90-93.
[19]Oxenkrug G.Serotonin-kynurenine hypothesis of depression:historical overview and recent developments[J].Curr Drug Targets,2013,14(5):514-521.
[20]Jouvet M.Sleep and serotonin:an unfinished story[J].Neuropsychopharmacology,1999,21(Suppl 2):24S-27S.
[21]杨岑,冉明梓,欧阳鹏荣,等.五羟色胺在睡眠-觉醒中作用[J].现代生物医学进展,2015,15(11):2191-2194.
[22]姚秋菊,韩旭.5-羟色胺与失眠的相关性及中医辨证治疗的研究进展[J].中医药导报,2013,19(2):108-110.
[23]Muguruza C,Moreno JL,Umali A,et al.Dysregulated 5-HT(2A)receptor binding in postmortem frontal cortex of schizophrenic subjects[J].Eur Neuropsychopharmacol,2013,23(8):852-864.
[24]Kaneda Y,Fujii A,Nagamine I.Platelet serotonin concentrations in medicated schizophrenic patients[J].Prog Neuropsychopharmacol Biol Psychiatry,2001,25(5):983-992.
[25]Fujimoto Y,Funao T,Suehiro K,et al.Brain serotonin content regulates the manifestation of tramadol-induced seizures in rats:disparity between tramadol-induced seizure and serotonin syndrome[J].Anesthesiology,2015,122(1):178-189.
[26]甘彦峰,刘广益.褪黑素对癫痫脑损伤神经保护作用的研究进展[J].医学综述,2007,13(17):1290-1292.
[27]Granger AJ,Mulder N,Saunders A,et al.Cotransmission of acetylcholine and GABA[J].Neuropharmacology,2016,100:40-46.
[28]Traini E,Bramanti V,Amenta F.Choline alphoscerate(alphaglyceryl-phosphoryl-choline)an old choline-containing phospholipid with a still interesting profile as cognition enhancing agent[J].Curr Alzheimer Res,2013,10(10):1070-1079.
[29]Nagy PM,Aubert I.Overexpression of the vesicular acetylcholine transporter enhances dendritic complexity of adult-born hippocampal neurons and improves acquisition of spatial memory during aging[J].Neurobiol Aging,2015,36(5):1881-1889.
[30]Gr9n G,Brandenburg I,Wunderlich AP,et al.Inhibition of hippocampal function in mild cognitive impairment:targeting the cholinergic hypothesis[J].Neurobio Aging,2006,27(1):78-87.
[31]李瑞芝,郭建友,李昌煜,等.焦虑症γ-氨基丁酸受体机制与药物干预研究进展[J].中国药理学通报,2010,26(9):1135-1138.
[32]M9hler H.Role of GABAAreceptors in cognition[J].Biochem Soc Trans,2009,37(Pt 6):1328-1333.
[33]赵庆杰,齐丹,孟珊珊,等.γ-羟基丁酸对脑缺血再灌注后海马区γ-氨基丁酸及其A型受体α1亚型的影响[J].中国组织工程研究,2005,9(45):37-39.
[34]Petroff OA,Hyder F,Mattson RH,et al.Topiramate increases brain GABA,homocarnosine,and pyrrolidinone in patients with epilepsy[J].Neurology,1999,52(3):473-478.
[35]Brown RE,Basheer R,Mc Kenna JT,et al.Control of sleep and wakefulness[J].Physiol Rev,2012,92(3):1087-1097.
[36]Coyle JT,Puttfarcken P.Oxidative stress,glutamate,and neurodegenerative disorders[J].Science,1993,262(5134):689-695.
[37]王琦,隋海娟,屈文慧,等.知母皂苷元对谷氨酸引起的皮层神经元损伤的保护作用研究[J].中国药理学通报,2013,29(2):281-285.
[38]Orset C,Parrot S,Sauvinet V,et al.Dopamine transporters are involved in the onset of hypoxia-induced dopamine efflux in striatum as revealed by in vivo microdialysis[J].Neurochem Int,2005,46(8):623-633.
[39]Ratajczak P,Kus K,Go?embiowska K,et al.The influence of aripiprazole and olanzapine on neurotransmitters level in frontal cortex of prenatally stressed rats[J].Environ Toxicol Pharmacol,2016,46:122-130.
[40]刘洋,李敏,侯悦,等.高效液相色谱-电化学法测定大鼠脑微透析液中多巴胺的方法建立及应用[J].药物分析杂志,2013,33(2):255-262.
[41]Nesbitt KM,Varner EL,Andrea JG,et al.Microdialysis in the rat striatum:effects of 24 h dexamethasone retrodialysis on evoked dopamine release and penetration injury[J].ACS Chem Neurosci,2015,6(1):163-173.
[42]Van SJ,Lens C,Maes K,et al.Reassessment of the antioxidative mixture for the challenging electrochemical determination of dopamine,noradrenaline and serotonin in microdialysis samples[J].J Chromatogr B Analyt Technol Biomed Life Sci,2015,998-999:63-71.
[43]Kamińska K,Goembiowska K,Rogóz6)Z.Effect of risperidone on the fluoxetine-induced changes in extracellular dopamine,serotonin and noradrenaline in the rat frontal cortex[J].Pharmacol Rep,2013,65(5):1144-1151.
[44]庄宗,赵旭东,史继新.微透析技术在神经外科中的应用[J].医学研究生学报,2010,23(8):880-883.
[45]Borland LM,Shi G,Yang H,et al.Voltammetric study of extracellular dopamine near microdialysis probes acutely implanted in the striatum of the anesthetized rat[J].J Neurosci Methods,2005,146(2):149-158.
[46]He Y,Zhao XE,Zhu S,et al.In situ derivatization-ultrasoundassisted dispersive liquid-liquid microextraction for the determination of neurotransmitters in Parkinson's rat brain microdialysates by ultra high performance liquid chromatography-tandem mass spectrometry[J].J Chromatogr A,2016(1458):70-81.
[47]Uutela P,ReinilR,Piepponen P,et al.Analysis of acetylcholine and choline in microdialysis samples by liquid chromatography/tandem mass spectrometry[J].Rapid Commun Mass Spectrom,2005,19(20):2950-2956.
[48]吕允凤,胡欣,毕开顺.应用微透析技术和质谱法测定川芎嗪对大鼠脑内乙酰胆碱释放量的影响[J].药学学报,2008,43(11):1128-1133.
[49]程发峰,宋文婷,邬金洋,等.精制清开灵注射液对脑缺血后大鼠脑微透析液中谷氨酸含量的影响[J].时珍国医国药,2015,26(3):582-584.
[50]Persike M,Zimmermann M,Klein J,et al.Quantitative determination of acetylcholine and choline in microdialysis samples by MALDI-TOF MS[J].Anal Chem,2010,82(3):922-929.
[51]郑芳昊,罗佳波.微透析采样考察麻黄-桂枝药对配伍对大鼠额叶皮层部位神经递质Glu和GABA水平的影响[J].时珍国医国药,2016,27(10):2335-2338.
[52]Jamal M,Ameno K,Wang W,et al.Inhibition of acetaldehyde metabolism decreases acetylcholine release in medial frontal cortex of freely moving rats[J].Brain Res,2005,1039(1-2):90-96.
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