高通量测序分析益生菌对冷食小鼠肠道菌群的干预
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摘要
采用高通量测序技术分析益生菌对冷食小鼠肠道菌群的影响。将实验小鼠随机分为模型组、对照组和低中高剂量组,分别灌喂等量的普通冰淇淋、107 cfu/m L益生菌液和107、108、109 cfu/m L的益生菌冰淇淋。连续灌喂15d,记录并计算小鼠的稀便率和腹泻指数,15 d后,收集各组小鼠粪便进行高通量测序分析。发现食用冷食会导致部分小鼠腹泻,在剂量范围内,益生菌可以缓解冷凉性敏感小鼠的腹泻症状,高剂量组小鼠的稀便率降低了3.11%,腹泻指数(10-1)降低了0.33,抑制效果较其它各组最为明显。服用了益生菌的小鼠粪便菌群多样性较高(高剂量组ACE值为69987.29,Chao1值为27098.77),乳酸菌属、艾克曼氏菌属、Blautia等菌属占比增大,菌群结构发生改变。因此益生菌可以提高冷凉性腹泻敏感小鼠对冷食的接受度,冷冻载体下的益生菌可以在小鼠肠道中定植并对小鼠肠道起到调节作用。
High-throughput sequencing was used to analyze the effects of probiotics on the intestinal flora of cold-fed mice. The experimental mice were randomly divided into a model group, a control group, and a low, medium, and high dose group, and an equal amount of ordinary ice cream, 107 cfu/m L probiotic liquid, and 107, 108, 109 cfu/m L probiotic ice cream, respectively. The rats were continuously fed for 15 days, and the stool rate and diarrhea index of mice were recorded and calculated. After 15 days, the feces of each group were collected for high-throughput sequencing analysis. It was found that cold food could cause diarrhea in some mice. In the dose range, probiotics could alleviate the symptoms of diarrhea in cold-sensitive mice. The high-dose group decreased the stool rate by 3.11% and the diarrhea index(10-1) by 0.33.The inhibition effect was significant, compared to the other groups. The fecal flora of mice with probiotics was highly diverse(the high-dose group had an ACE value of 69987.29 and the Chao1 value was 27098.77), and the proportion of genus Lactobacillus, Ekmenia, and Blautia increased and their structure changed. Therefore, probiotics could improve the acceptance of cold food in cold-sensitive diarrhea-sensitive mice.The probiotics under the frozen carrier could be colonized in the intestine of mice and regulate the intestinal tract of mice.
High-throughput sequencing was used to analyze the effects of probiotics on the intestinal flora of cold-fed mice. The experimental mice were randomly divided into a model group, a control group, and a low, medium, and high dose group, and an equal amount of ordinary ice cream, 107 cfu/m L probiotic liquid, and 107, 108, 109 cfu/m L probiotic ice cream, respectively. The rats were continuously fed for 15 days, and the stool rate and diarrhea index of mice were recorded and calculated. After 15 days, the feces of each group were collected for high-throughput sequencing analysis. It was found that cold food could cause diarrhea in some mice. In the dose range, probiotics could alleviate the symptoms of diarrhea in cold-sensitive mice. The high-dose group decreased the stool rate by 3.11% and the diarrhea index(10-1) by 0.33.The inhibition effect was significant, compared to the other groups. The fecal flora of mice with probiotics was highly diverse(the high-dose group had an ACE value of 69987.29 and the Chao1 value was 27098.77), and the proportion of genus Lactobacillus, Ekmenia, and Blautia increased and their structure changed. Therefore, probiotics could improve the acceptance of cold food in cold-sensitive diarrhea-sensitive mice.The probiotics under the frozen carrier could be colonized in the intestine of mice and regulate the intestinal tract of mice.
引文
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[3]Ringel Y,Maharshak N.Intestinal microbiota and immune function in the pathogenesis of irritable bowel syndrome[J].American Journal of Physiology Gastrointestinal&Liver Physiology,2013,305(8):G529
[4]Ford A C,Lacy B E,Talley N J.Irritable bowel syndrome[J].New England Journal of Medicine,2017,376(26):2566-2578
[5]Qin S Y,Jiang H X,Lu D H,et al.Association of interleukin-10 poly-morphisms with risk of irritable bowel syndrome:A meta-analysis[J].World J Gastroenterol,2013,19(48):9472-9480
[6]Holtmann G J,Ford A C,Talley N J.Pathophysiology of irritable bowel syndrome[J].Lancet Gastroenterology&Hepatology,2016,1(2):133-146
[7]Ringel Y,Ringelkulka T.The intestinal microbiota and irritable bowel syndrome[J].Journal of Clinical Gastroenterology,2015,49(1):56
[8]Yu Z,Kong M,Zhang P,et al.Immune-enhancing activity of extracellular polysaccharides isolated from Rhizopus nigricans[J].Carbohydrate Polymers,2016,148
[9]Aboulfazli F,Shori A B,Baba A S.Effects of the replacement of cow milk with vegetable milk on probiotics and nutritional profile of fermented ice cream[J].LWT-Food Science and Technology,2016,70:261-270
[10]Jingjie Li,Wen Zhang,Chuan Wang,et al.Lactococcus lactis expressing food-gradeβ-galactosidase alleviates lactose intolerance symptoms in post-weaning Balb/c mice[J].Applied Microbiology&Biotechnology,2012,96(6):1499-1506
[11]Savino F,Fornasero S,Ceratto S,et al.Probiotics and gut health in infants:A preliminary case-control observational study about early treatment with Lactobacillus reuteri DSM17938[J].Clinica Chimica Acta,2015,451:82-87
[12]曾令容,李荣萍,陈青云,等.益生菌制剂对腹泻型肠易激综合征的防治作用[J].局解手术杂志,2011,20(4):430-432ZENG Ling-rong,LI Rong-ping,CHEN Qing-yun,et al.Preventive and therapeutic effect of probiotic preparation on diarrhea-predominant irritable bowel syndrome[J].J Reg Anat Oper Surg,2011,20(4):430-432
[13]Eleonora Distrutti,Lorenzo Monaldi,Patrizia Ricci,et al.Gut microbiota role in irritable bowel syndrome:New therapeutic strategies[J].World Journal of Gastroenterology,2016,22(7):2219-2241
[14]曲巍,张智,马建章,等.高通量测序研究益生菌对小鼠肠道菌群的影响[J].食品科学,2017,38(1):214-219QU Wei,ZHANG Zhi,MA Jian-zhang,et al.Effects of probiotics on intestinal flora in mice by high-throughput sequencing[J].Food Science,2017,38(1):214-219
[15]Tina Didari,Shilan Mozaffari,Shekoufeh Nikfar,et al.Effectiveness of probiotics in irritable bowel syndrome:Updated systematic review with meta-analysis[J].World Journal of Gastroenterology,2015,21(10):3072-3084
[16]Cong Dai,Chang-Qing Zheng,Min Jiang,et al.Probiotics and irritable bowel syndrome[J].World Journal of Gastroenterology,2013,19(36):5973-5980
[17]Meizhong H,Haizhen Z,Chong Z,et al.Purification and characterization of plantaricin 163,a novel bacterioncin produced by Lactobacillus Plantarum 163 isolated from traditional Chinese fermented vegetables[J].J.Agric Food Chem,2013,61(47):11676-11682
[18]张智,林秀芳,马建章,等.玉米肽对双歧杆菌免疫力作用的影响[J].食品研究与开发,2017,38(3):9-14ZHANG Zhi,LIN Xiu-fang,MA Jian-zhang,et al.Effects of corn peptide on the immunity of bifidobacteria[J].Food Research and Development,2017,38(3):9-14
[19]Eleonora Distrutti,Lorenzo Monaldi,Patrizia Ricci,et al.Gut microbiota role in irritable bowel syndrome:New therapeutic strategies[J].World Journal of Gastroenterology,2016,22(7):2219-2241
[20]谯仕彦,侯成立,曾祥芳.乳酸菌对猪肠道屏障功能的调节作用及其机制[J].动物营养学报,2014,26(10):3052-3063QIAO Shi-yan,HOU Cheng-li,ZENG Xiang-fang.Regulatory effect of Lactic acid bacteria on intestinal barrier function of pigs and its mechanism[J].Journal of Animal Nutrition,2014,26(10):3052-3063
[21]Maynatd C L,Elson C O,Hatton R D,et al.Reciprocal interactions of the intestinal microbiota and immune system[J].Nature,2012,489(7415):231-241
[22]Guilloteau P,Martin L,Eeckhaut V.et al.From the gut to the peripheral tissues:the multiple effects of butyrate[J].Nutrition Research Reviews,2010,23(2):366-384
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