光合细菌菌剂和沼泽红假单胞菌对实验水体氮磷营养盐和微生物群落的影响
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摘要
为比较光合细菌菌剂与沼泽红假单胞菌(Rhodopseudomonas palustris)的生理生态特性,分析了不同初始菌量的菌剂PG和菌株PSB-1对实验水体氨氮(NH_4~+-N)、亚硝氮(NO_2~--N)、硝氮(NO_3~--N)和活性磷(PO_4~(3-)-P)的降解效果,通过高通量测序分析了菌剂PG的优势菌组成及实验结束时水体细菌数量和微生物群落组成。结果显示,菌剂PG组对实验水体的PO_4~(3-)-P、NO_3~--N和NO_2~--N有一定的降解作用,其最大降解率分别为40.98%、28.28%和20.12%。菌株PSB-1组仅对实验水体的NO_2~--N和PO_4~(3-)-P有一定的降解效果,其最大降解率分别为14.19%和9.88%。菌剂PG的主要优势菌为红假单胞菌属(Rhodopseudomonas sp.)。实验7 d后实验组水体细菌数量和微生物群落结构发生变化,水体细菌数量增长,形成以异养细菌为优势菌的菌群结构。结果表明光合细菌菌剂PG对水质因子的降解效果优于沼泽红假单胞菌PSB-1,但与报道的高效光合细菌菌株的降解能力存在一定差距。
To compare the physiological and ecological characteristics of inoculant of photosynthetic bacteria and Rhodopseudomonas palustri, we analyzed the degradation effects of the inoculant PG and the strain PSB-1 with different initial amounts of bacteria on ammonia nitrogen( NH+4-N), nitrite nitrogen(NO_2~--N), nitrate nitrogen( NO_3~--N) and phosphate(PO_4~3-P) in experimental water.Then we studied the dominant bacteria composition of inoculant of photosynthetic bacteria PG, as well as the number of bacteria and microbial community composition in experimental water at the end of the experiment by high-throughput sequencing. The results show that the inoculant PG group had certain degradation effects on PO3-4-P, NO_3~--N and NO_2~--N in experimental water. The maximum degradation rates of PG for PO3--P,-N and 4 NO-3 NO_2~--N were 40.98%, 28.28% and 20.12%, respectively. Moreover, the strain PSB-1 group only had certain degradation effect on NO_2~--N and PO_4~3-P. The maximum degradation rates of PSB-1 were 14.19% and9.88%, respectively. The dominant bacteria of the inoculant PG belonged to Rhodopseudomonas sp.. After 7 d of experiment, the number of bacteria and microbial community structure in the experimental group changed, and the number of bacteria in the water increased, forming a microbial structure with heterotrophic bacteria as the dominant bacteria. It is indicated that the inoculant of photosynthetic bacteria PG has better effect on the degradation of water quality factors than R. palustris PSB-1. However, there is a certain gap between both things and the reported degradation efficiency of photosynthetic bacterial strains.
To compare the physiological and ecological characteristics of inoculant of photosynthetic bacteria and Rhodopseudomonas palustri, we analyzed the degradation effects of the inoculant PG and the strain PSB-1 with different initial amounts of bacteria on ammonia nitrogen( NH+4-N), nitrite nitrogen(NO_2~--N), nitrate nitrogen( NO_3~--N) and phosphate(PO_4~3-P) in experimental water.Then we studied the dominant bacteria composition of inoculant of photosynthetic bacteria PG, as well as the number of bacteria and microbial community composition in experimental water at the end of the experiment by high-throughput sequencing. The results show that the inoculant PG group had certain degradation effects on PO3-4-P, NO_3~--N and NO_2~--N in experimental water. The maximum degradation rates of PG for PO3--P,-N and 4 NO-3 NO_2~--N were 40.98%, 28.28% and 20.12%, respectively. Moreover, the strain PSB-1 group only had certain degradation effect on NO_2~--N and PO_4~3-P. The maximum degradation rates of PSB-1 were 14.19% and9.88%, respectively. The dominant bacteria of the inoculant PG belonged to Rhodopseudomonas sp.. After 7 d of experiment, the number of bacteria and microbial community structure in the experimental group changed, and the number of bacteria in the water increased, forming a microbial structure with heterotrophic bacteria as the dominant bacteria. It is indicated that the inoculant of photosynthetic bacteria PG has better effect on the degradation of water quality factors than R. palustris PSB-1. However, there is a certain gap between both things and the reported degradation efficiency of photosynthetic bacterial strains.
引文
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[12]MUKHOPADHYAY M, CHAKRAVARTY D. Application of phototrophic purple non sulfur bacteria as fish feed for aquarium fishes[J]. J Environ Sociobiol, 2015(1):21-21.
[13]黄雪娇,杨冲,倪九派,等.1株高效去除氨氮的红假单胞菌的分离鉴定及特性[J].环境科学,2016, 37(6):2276-2283.
[14]JIN H, ZHAO Y, LEI X C, et al. Treat shrimp wastewater with compound photosynthetic bacteria[C]//International Conference on Computer Distributed Control and Intelligent Environmental Monitoring(CDCIEM). Changsha:IEEE, 2011:2352-2355.
[15]冯亚丽.海洋光合细菌在水产养殖中的应用基础研究[D]·哈尔滨:哈尔滨工业大学,2017:40-46.
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[19]文刚,汪彬,刘标,等.沼泽红假单胞菌R-3去除水体中氨氮的特性研究[J].湖南农业科学,2017(6):49-51.
[20]陈燕红,杨紫红,喻国辉,等.光照、氧气、pH和盐度对沼泽红假单胞菌2-8菌株生长和亚硝酸盐消除的影响[J].南方水产,2010,6(4):1-5.
[21]雷爱莹,彭敏,曾地刚,等.高效净水沼泽红假单胞菌的分离和鉴定[J].广西农业科学,2005, 36(1):57-58.
[22]袁翠霖.罗非鱼养殖系统微生物群落生态研究及潜在有益菌的筛选[D].广州:暨南大学,2011:50.
[23]胡晓娟,曹煜成,文国樑,等.罗非鱼养殖系统潜在有机物降解菌的筛选及其降解能力测定[J].农业环境科学学报,2014, 33(6):1233-1239.
[24]徐之文.水质指标测定与分析及在水产养殖中的应用[J].吉林畜牧兽医,2014, 35(5):18-20.
[25]李卓佳,李奕雯,曹煜成,等.凡纳滨对虾海水高位池养殖水体理化因子变化与营养状况分析[J].农业环境科学学报,2010,29(10):2025-2032.
[26]BRENNER D J, KRIEG N R, STALEY J T. Bergey's manual of systematic bacteriology[M]. 2th ed. New York:Springer, 2005:1-474.
[27]黄雪娇,杨冲,罗雅雪,等.光合细菌在水污染治理中的研究进展[J].中国生物工程杂志,2014, 34(11):119-124.
[28]刘洋,舒巧玉,楼春燕,等.两株光合细菌的分离鉴定及其水质净化效果研究[J].淡水渔业,2013, 43(5):57-61.
[29]秦宇胜,张君枝,马文林,等.北京地表水体光合细菌生长及脱氮除磷特性研究[J].环境工程,2015(s1):291-294.
[30]董艳珍,亓东明.一株光合细菌的净水能力分析[J].西昌学院学报(自然科学版),2013(3):13-15.
[31]ZHANG J, ZHANG W X, LI S Z, et al. A two-step fermentation of distillers'grains using Trichoderma viride and Rhodopseudomonas palustris for fish feed[J]. Bioprocess Biosyst Eng, 2013,36(10):1435-1443.
[32]ZHOU X X, PAN Y J, WANG Y B, et al. In vitro assessment of gastrointestinal viability of two photosynthetic bacteria,Rhodopseudomonas palustris and Rhodobacter sphaeroides[J]. J Zhejiang Univ Sci B, 2007, 8(9):686-692.
[33]QI Z Z,ZHANG X H,BOON N,et al. Probiotics in aquaculture of China:current state, problems and prospect[J]. Aquaculture,2009, 290(1/2):15-21.
[34]林启存,朱丽敏,沈小红,等.一株光合细菌的分离与鉴定[J].浙江农业科学,2017, 58(8):1444-1446.
[35]ZHANG X, SHU M, WANG Y, et al. Effect of photosynthetic bacteria on water quality and microbiota in grass carp culture[J].World J Microbiol Biotechnol, 2014, 30(9):2523-2531.
[36]杨鸾鸾,曹煜成,李卓佳,等.PS1沼泽红假单胞菌对集约化对虾养殖废水的净化作用[J].中国微生态学杂志,2009, 21(1):4-6.
[37]李莎莎,曹煜成,胡晓娟,等.响应面法优化芽孢杆菌(Bacillus sp.)A4的培养参数[J].南方水产科学,2017, 13(5):85-93.
[38]张修建.光合细菌制剂在水产养殖应用中需注意的问题[J].河北渔业,2008(11):8-9.
[2]彭智平,李文英,徐培智,等.光合菌剂对菜心产量和品质的效应评价[J].广东农业科学,2012, 39(1):11-13.
[3]BANEEJEE G, RAY A K. The advancement of probiotics research and its application in fish farming industries[J]. Rese Vet Sci,2017, 115:66-77.
[4]FARZANFAR A. The use of probiotics in shrimp aquaculture[J].Ferns Immuno Med Microbiol, 2006, 48(2):149-158.
[5]JEONG S K, CHO J S, KONG I S, et al. Purification of aquarium water by PVA gel-immobilized photosynthetic bacteria during goldfish rearing[J]. Biotechnol Bioproc Engin, 2009, 14(2):238-247.
[6]SHIUNG L L, CHANG M J, CHANG Y T, et al. Photosynthetic purple sulfur bacterium Marichromatium RuA2 induces changes in water quality parameters, the occurrence of sulfonamide resistance gene and microbial community structure of marine aquaculture[J]. Aquaculture, 2018, 493:68-78.
[7]丁雷,赵德炳.光合细菌在水产养殖上的应用研究与进展[J].水利渔业,2001,21(1):23-25.
[8]KIM J K, LEE B K. Mass production of Rhodopseudomonas palustris as diet for aquaculture[J]. Aquacult Engin, 2000, 23(4):281-293.
[9]SHAPLEIGH J P. Dissimilatory and assimilatory nitrate reduction in the purple photosynthetic bacteria[M]. Dordrecht, Netherlands:Springer, 2009:623-642.
[10]王树香,吴玉华,杨炳武,等.淡水养殖净化菌株的筛选及其对水质的改善效果[J].贵州农业科学,2014(3):98-101.
[11]杨官娥,张肇铭,赵利华,等.神奇的光合细菌菌剂[J].农产品加工, 2010(3):8-9.
[12]MUKHOPADHYAY M, CHAKRAVARTY D. Application of phototrophic purple non sulfur bacteria as fish feed for aquarium fishes[J]. J Environ Sociobiol, 2015(1):21-21.
[13]黄雪娇,杨冲,倪九派,等.1株高效去除氨氮的红假单胞菌的分离鉴定及特性[J].环境科学,2016, 37(6):2276-2283.
[14]JIN H, ZHAO Y, LEI X C, et al. Treat shrimp wastewater with compound photosynthetic bacteria[C]//International Conference on Computer Distributed Control and Intelligent Environmental Monitoring(CDCIEM). Changsha:IEEE, 2011:2352-2355.
[15]冯亚丽.海洋光合细菌在水产养殖中的应用基础研究[D]·哈尔滨:哈尔滨工业大学,2017:40-46.
[16]布坎南R E,吉本斯N E.伯杰氏细菌鉴定手册[M]. 8版:北京:科学出版社,1984:1-22.
[17]郑卉.一株光合细菌对养殖水水质调控及其在水体中的动态变化研究[D].宁波:宁波大学,2012:15.
[18]张晓波,朱笔通,熊慧,等.沼泽红假单胞菌(Rhodopseudomonas palustris)CQV97对无机三态氮共存水体中氮素的去除效率及其影响因素[J].氨基酸和生物资源,2015, 37(4):38-45.
[19]文刚,汪彬,刘标,等.沼泽红假单胞菌R-3去除水体中氨氮的特性研究[J].湖南农业科学,2017(6):49-51.
[20]陈燕红,杨紫红,喻国辉,等.光照、氧气、pH和盐度对沼泽红假单胞菌2-8菌株生长和亚硝酸盐消除的影响[J].南方水产,2010,6(4):1-5.
[21]雷爱莹,彭敏,曾地刚,等.高效净水沼泽红假单胞菌的分离和鉴定[J].广西农业科学,2005, 36(1):57-58.
[22]袁翠霖.罗非鱼养殖系统微生物群落生态研究及潜在有益菌的筛选[D].广州:暨南大学,2011:50.
[23]胡晓娟,曹煜成,文国樑,等.罗非鱼养殖系统潜在有机物降解菌的筛选及其降解能力测定[J].农业环境科学学报,2014, 33(6):1233-1239.
[24]徐之文.水质指标测定与分析及在水产养殖中的应用[J].吉林畜牧兽医,2014, 35(5):18-20.
[25]李卓佳,李奕雯,曹煜成,等.凡纳滨对虾海水高位池养殖水体理化因子变化与营养状况分析[J].农业环境科学学报,2010,29(10):2025-2032.
[26]BRENNER D J, KRIEG N R, STALEY J T. Bergey's manual of systematic bacteriology[M]. 2th ed. New York:Springer, 2005:1-474.
[27]黄雪娇,杨冲,罗雅雪,等.光合细菌在水污染治理中的研究进展[J].中国生物工程杂志,2014, 34(11):119-124.
[28]刘洋,舒巧玉,楼春燕,等.两株光合细菌的分离鉴定及其水质净化效果研究[J].淡水渔业,2013, 43(5):57-61.
[29]秦宇胜,张君枝,马文林,等.北京地表水体光合细菌生长及脱氮除磷特性研究[J].环境工程,2015(s1):291-294.
[30]董艳珍,亓东明.一株光合细菌的净水能力分析[J].西昌学院学报(自然科学版),2013(3):13-15.
[31]ZHANG J, ZHANG W X, LI S Z, et al. A two-step fermentation of distillers'grains using Trichoderma viride and Rhodopseudomonas palustris for fish feed[J]. Bioprocess Biosyst Eng, 2013,36(10):1435-1443.
[32]ZHOU X X, PAN Y J, WANG Y B, et al. In vitro assessment of gastrointestinal viability of two photosynthetic bacteria,Rhodopseudomonas palustris and Rhodobacter sphaeroides[J]. J Zhejiang Univ Sci B, 2007, 8(9):686-692.
[33]QI Z Z,ZHANG X H,BOON N,et al. Probiotics in aquaculture of China:current state, problems and prospect[J]. Aquaculture,2009, 290(1/2):15-21.
[34]林启存,朱丽敏,沈小红,等.一株光合细菌的分离与鉴定[J].浙江农业科学,2017, 58(8):1444-1446.
[35]ZHANG X, SHU M, WANG Y, et al. Effect of photosynthetic bacteria on water quality and microbiota in grass carp culture[J].World J Microbiol Biotechnol, 2014, 30(9):2523-2531.
[36]杨鸾鸾,曹煜成,李卓佳,等.PS1沼泽红假单胞菌对集约化对虾养殖废水的净化作用[J].中国微生态学杂志,2009, 21(1):4-6.
[37]李莎莎,曹煜成,胡晓娟,等.响应面法优化芽孢杆菌(Bacillus sp.)A4的培养参数[J].南方水产科学,2017, 13(5):85-93.
[38]张修建.光合细菌制剂在水产养殖应用中需注意的问题[J].河北渔业,2008(11):8-9.
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