海南南繁区水稻纹枯病菌的遗传多样性与致病力分化
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摘要
【目的】为明确南繁区水稻纹枯病菌(RhizoctoniasolaniAG-1IA)的遗传分化及遗传多样性与致病力的关系,【方法】采用AFLP分子标记技术和离体叶片接种法对南繁核心区(三亚、乐东、陵水)和非核心区(琼中、屯昌和定安)共60株水稻纹枯病菌的遗传多样性、遗传结构和致病力分化进行了测定,并分析了遗传多样性与致病力之间的关系。【结果】聚类分析结果表明,核心区菌株的遗传多样性相对更高;群体遗传结构分析表明,核心区群体的多态性位点百分率(PPL)、Nei基因多样性指数(H)、Shannon信息指数(I)和基因流(N_m)分别为82.24%、0.1932、0.3062和2.5627,高于非核心区群体的67.49%、0.1535、0.2447和0.9365;而核心区群体的基因分化系数(G_(st))为0.1633,低于非核心区群体的0.3481。【结论】核心区菌株的遗传变异程度比非核心区菌株高;核心区不同群体间存在较多的基因交流,而非核心区菌体间的基因交流较少,但遗传变异均主要来自于群体内;总体而言,核心区菌株的遗传分化程度更高。菌株的致病力及其与菌株遗传多样性的相关性分析表明,核心区菌株以中等致病型为主,而非核心区菌株则以中、强致病型为主,但与菌株的AFLP谱系之间的相关均未达显著水平。
【Objective】In order to clarify the genetic differentiation and the correlation between genetic diversity and pathogenicity of Rhizoctonia solani AG-1 ⅠA in South China Crop Breeding Area, the genetic diversity, population genetic structure, pathogenicity and the correlation between genetic diversity and pathogenicity of 60 R. solani AG-1 IA isolates collected from the core region(Sanya, Ledong and Lingshui) and non-core region(Qiongzhong, Tunchang and Ding'an) of South China Crop Breeding Area were comparatively analyzed 【Method】by using AFLP molecular marker technique and detached leaf inoculation method. 【Result】The cluster analysis results showed that the genetic diversity of R. solani AG-1 ⅠA in the core region was relatively higher. The analysis results of population genetic structure showed that the percentage of polymorphic loci(PPL), Nei's gene diversity index(H), Shannon's information index(I) and gene flow(N_m) in the core population were 82.24%, 0.1932, 0.3062 and 2.5627, respectively, higher than those of non-core population with PPL, H, I and Nm being 67.49%, 0.1535, 0.2447 and 0.9365, respectively. However,the genetic differentiation coefficient(G_(st)) in the core population was 0.1633, lower than that of non-core population whose G_(st) was 0.3481. 【Conclusion】These results showed that the degree of genetic variation of the isolates from core region was higher than that of the isolates from non-core region. Much gene flow among the core populations and little gene flow among the non-core populations existed, but the genetic variation of both regions was mainly within the population. Overall, the degree of genetic differentiation in the isolates of core region was higher. The results of pathogenicity and correlation between genetic diversity and pathogenicity showed that most isolates from core region belonged to the medium pathotypes and most isolates from non-core region belonged to the medium or strong pathotypes, but there was no correlation between pathogenicity and AFLP lineage.
【Objective】In order to clarify the genetic differentiation and the correlation between genetic diversity and pathogenicity of Rhizoctonia solani AG-1 ⅠA in South China Crop Breeding Area, the genetic diversity, population genetic structure, pathogenicity and the correlation between genetic diversity and pathogenicity of 60 R. solani AG-1 IA isolates collected from the core region(Sanya, Ledong and Lingshui) and non-core region(Qiongzhong, Tunchang and Ding'an) of South China Crop Breeding Area were comparatively analyzed 【Method】by using AFLP molecular marker technique and detached leaf inoculation method. 【Result】The cluster analysis results showed that the genetic diversity of R. solani AG-1 ⅠA in the core region was relatively higher. The analysis results of population genetic structure showed that the percentage of polymorphic loci(PPL), Nei's gene diversity index(H), Shannon's information index(I) and gene flow(N_m) in the core population were 82.24%, 0.1932, 0.3062 and 2.5627, respectively, higher than those of non-core population with PPL, H, I and Nm being 67.49%, 0.1535, 0.2447 and 0.9365, respectively. However,the genetic differentiation coefficient(G_(st)) in the core population was 0.1633, lower than that of non-core population whose G_(st) was 0.3481. 【Conclusion】These results showed that the degree of genetic variation of the isolates from core region was higher than that of the isolates from non-core region. Much gene flow among the core populations and little gene flow among the non-core populations existed, but the genetic variation of both regions was mainly within the population. Overall, the degree of genetic differentiation in the isolates of core region was higher. The results of pathogenicity and correlation between genetic diversity and pathogenicity showed that most isolates from core region belonged to the medium pathotypes and most isolates from non-core region belonged to the medium or strong pathotypes, but there was no correlation between pathogenicity and AFLP lineage.
引文
[1]陈冠铭,李劲松,曹兵.发挥南繁资源优势,促进种业科技创新.安徽农学通报,2012,18(1):27-29.Chen G M,Li J S,Cao B.Make the most of resource advantages of Hainan national multiplication,promoting sci-tech innovations of seed industry.Anhui Agric Sci Bull,2012,18(1):27-29.(in Chinese with English abstract)
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[3]中国植物保护学会.2018年农作物重大病虫害重于往年,累计将发生50亿亩次[EB/OL].http://mp.weixin.qq.com/s/8c Rq7ohk SH1HAz HiE-qiIA,2018-01-12.Chinese Society of Plant Protection.The major diseases and insect pests of crops are more severe than those in previous years,and a total of 5 billion mu will occur[EB/OL].http://mp.weixin.qq.com/s/8cRq 7ohkSH1HAz HiE-qiIA:2018-01-12.(in Chinese with English abstract)
[4]舒灿伟,曹琦琦,赵美,江绍锋,周而勋.立枯丝核菌不同融合群G蛋白β亚基基因克隆与分子进化分析.华北农学报,2017,32(5):7-12.Shu C W,Cao Q Q,Zhao M,Jiang S F,Zhou E X.Cloning and sequence analysis of G-protein beta-subunit genes in different anastomosis groups of Rhizoctonia solani.Acta Agric Boreali-Sin,2017,32(5):7-12.(in Chinese with English abstract)
[5]Feng S J,Shu C W,Wang C J Z,Jiang S F,Zhou E X.Survival of Rhizoctonia solani AG-1 IA,the causal agent of rice sheath blight,under different environmental conditions.J Phytopathol,2017,165(1):44-52.
[6]周而勋,曹菊香,杨媚,朱西儒.我国南方六省(区)水稻纹枯病菌遗传多样性的研究.南京农业大学学报,2002,25(3):36-40.Zhou E X,Cao J X,Yang M,Zhu X R.Studies on the genetic diversity of Rhizoctonia solani AG-1-IA from six provinces in the southern China.J Nanjing Agric Univ,2002,25(3):36-40.(in Chinese with English abstract)
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[8]王玲,左示敏,张亚芳,陈宗祥,黄世文,潘学彪.中国南方八省(自治区)水稻纹枯病菌群体遗传结构的SSR分析.中国农业科学,2015,48(13):2538-2548.Wang L,Zuo S M,Zhang Y F,Chen Z X,Huang S W,Pan X B.SSR analysis of population genetic structure of rice sheath blight causing agent Rhizoctonia solani AG1-IA collected from eight provinces(Autonomous Region)in southern China.Sci Agric Sin,2015,48(13):2538-2548.(in Chinese with English abstract)
[9]Shu C W,Zou C J,Chen J L,Tang F,Yi R H,Zhou E X.Genetic diversity and population genetic structure of Rhizoctonia solani AG-1 IA,the causal agent of rice sheath blight,in south China.Can J Plant Pathol,2014,36(2):179-186.
[10]Taheri P,Gnanamanickam S,H?fte M.Characterization,genetic structure,and pathogenicity of Rhizoctonia spp.associated with rice sheath diseases in India.Phytopathology,2007,97(3):373-383.
[11]范文艳,文景芝,金丽娜,马建,陈瑾.黑龙江省水稻纹枯病菌的致病力分化与AFLP分析.植物保护,2008,34(6):57-61.Fan W Y,Wen J Z,Jin L N,Ma J,Chen J.Pathogenic differentiation and AFLP analysis of the rice sheath blight strains from Heilongjiang.Plant Prot,2008,34(6):57-61.(in Chinese with English abstract)
[12]朱名海,皮磊,舒灿伟,周而勋.南繁区稻瘟病菌遗传多样性和群体遗传结构的AFLP分析.中国水稻科学,2017,31(3):320-326.Zhu M H,Pi L,Shu C W,Zhou E X.AFLP analyses of genetic diversity and population genetic structure of Magnaporthe oryzae from South China Crop Breeding Area.Chin J Rice Sci,2017,31(3):320-326.(in Chinese with English abstract)
[13]周而勋,杨媚.从植物病组织中分离丝核菌的快速、简便技术.华南农业大学学报,1998,19(1):125-126.Zhou E X,Yang M.A rapid and simple technique for the isolation of Rhizoctonia solani from diseased plant tissues.J South China Agric Univ,1998,19(1):125-126.(in Chinese with English abstract)
[14]黄江华,杨媚,周而勋,戚佩坤.丝核菌细胞核染色技术的研究.仲恺农业技术学院学报,2001,14(4):13-17.Huang J H,Yang M,Zhou E X,Qi P K.Studies on the nuclear staining techniques for Rhizoctonia spp.JZhongkai Agrotechnol Coll,2001,14(4):13-17.(in Chinese with English abstract)
[15]周而勋,杨媚.广东省水稻纹枯病菌的致病力和融合群研究.广东农业科学,1999,26(5):36-38.Zhou E X,Yang M.Studies on the pathogenicity and anastomosis group of Rhizoctonia solani in Guangdong province.Guangdong Agric Sci,1999,26(5):36-38.(in Chinese)
[16]Brant J B,Gustavo C A,Peter M G.Fast and sensitive silver staining of DNA in polyacrylamide gels.Anal Biochem,1991,196(1):80-83.
[17]陈涛,张震,柴荣耀,王教瑜,毛雪琴,邱海萍,杜新法,姜华,王立安,王艳丽,孙国昌.浙江省水稻纹枯病菌的遗传分化与致病力研究.中国水稻科学,2010,24(1):67-72.Chen T,Zhang Z,Chai R Y,Wang J Y,Mao X Q,Qiu HP,Du X F,Jiang H,Wang L A,Wang Y L,Sun G C.Genetic diversity and pathogenicity variation of different Rhizoctonia solani isolates in rice from Zhejiang Province,China.Chin J Rice Sci,2010,24(1):67-72.(in Chinese with English abstract)
[18]黄雯雯,王玲,刘连盟,刘恩勇,黄世文.安徽省水稻纹枯病菌致病力分化与遗传多样性.浙江农业学报,2011,23(1):111-116.Huang W W,Wang L,Liu L M,Liu E Y,Huang S W.Preliminary study on the pathogenic differentiation and genetic diversity of rice sheath blight pathogen strains from Anhui Province.Acta Agric Zhejiang,2011,23(1):111-116.(in Chinese with English abstract)
[19]吴荷芳,王晓宇,罗楚平,陈志谊.江苏省水稻纹枯病菌遗传多样性分析与致病力研究.江苏农业学报,201329(1):51-59.Wu H F,Wang X Y,Luo C P,Chen Z Y.Genetic diversity and pathogenicity of Rhizoctonia solani isolates from Jiangsu province.Jiangsu J Agric Sci,2013 29(1):51-59.(in Chinese with English abstract)
[20]张优,魏松红,王海宁,李帅,李思博,罗文芳.东北地区水稻纹枯病菌遗传多样性和致病性分析.沈阳农业大学学报,2017,48(1):9-14.Zhang Y,Wei S H,Wang H N,Li S,Li S B,Luo W F.Genetic diversity and pathogenicity of Rhizoctonia solani isolates causing sheath blight disease of rice in northeast region.J Shenyang Agric Univ,2017,48(1):9-14.(in Chinese with English abstract)
[2]Moni Z R,Ali M A,Alam M S,Rahman M A,Bhuiyan M R,Mian M S,Iftekharuddaula K M,Latif M A,IqbalKhan M A.Morphological and genetic variability among Rhizoctonia solani isolates causing sheath blight disease of rice.Rice Sci,2016,23(1):42-50.
[3]中国植物保护学会.2018年农作物重大病虫害重于往年,累计将发生50亿亩次[EB/OL].http://mp.weixin.qq.com/s/8c Rq7ohk SH1HAz HiE-qiIA,2018-01-12.Chinese Society of Plant Protection.The major diseases and insect pests of crops are more severe than those in previous years,and a total of 5 billion mu will occur[EB/OL].http://mp.weixin.qq.com/s/8cRq 7ohkSH1HAz HiE-qiIA:2018-01-12.(in Chinese with English abstract)
[4]舒灿伟,曹琦琦,赵美,江绍锋,周而勋.立枯丝核菌不同融合群G蛋白β亚基基因克隆与分子进化分析.华北农学报,2017,32(5):7-12.Shu C W,Cao Q Q,Zhao M,Jiang S F,Zhou E X.Cloning and sequence analysis of G-protein beta-subunit genes in different anastomosis groups of Rhizoctonia solani.Acta Agric Boreali-Sin,2017,32(5):7-12.(in Chinese with English abstract)
[5]Feng S J,Shu C W,Wang C J Z,Jiang S F,Zhou E X.Survival of Rhizoctonia solani AG-1 IA,the causal agent of rice sheath blight,under different environmental conditions.J Phytopathol,2017,165(1):44-52.
[6]周而勋,曹菊香,杨媚,朱西儒.我国南方六省(区)水稻纹枯病菌遗传多样性的研究.南京农业大学学报,2002,25(3):36-40.Zhou E X,Cao J X,Yang M,Zhu X R.Studies on the genetic diversity of Rhizoctonia solani AG-1-IA from six provinces in the southern China.J Nanjing Agric Univ,2002,25(3):36-40.(in Chinese with English abstract)
[7]Wang L,Liu L M,Wang Z G,Huang S W.Genetic structure and aggressiveness of Rhizoctonia solani AG1-IA,the cause of sheath blight of rice in southern China.JPhytopathol,2013,161(11-12):753-762.
[8]王玲,左示敏,张亚芳,陈宗祥,黄世文,潘学彪.中国南方八省(自治区)水稻纹枯病菌群体遗传结构的SSR分析.中国农业科学,2015,48(13):2538-2548.Wang L,Zuo S M,Zhang Y F,Chen Z X,Huang S W,Pan X B.SSR analysis of population genetic structure of rice sheath blight causing agent Rhizoctonia solani AG1-IA collected from eight provinces(Autonomous Region)in southern China.Sci Agric Sin,2015,48(13):2538-2548.(in Chinese with English abstract)
[9]Shu C W,Zou C J,Chen J L,Tang F,Yi R H,Zhou E X.Genetic diversity and population genetic structure of Rhizoctonia solani AG-1 IA,the causal agent of rice sheath blight,in south China.Can J Plant Pathol,2014,36(2):179-186.
[10]Taheri P,Gnanamanickam S,H?fte M.Characterization,genetic structure,and pathogenicity of Rhizoctonia spp.associated with rice sheath diseases in India.Phytopathology,2007,97(3):373-383.
[11]范文艳,文景芝,金丽娜,马建,陈瑾.黑龙江省水稻纹枯病菌的致病力分化与AFLP分析.植物保护,2008,34(6):57-61.Fan W Y,Wen J Z,Jin L N,Ma J,Chen J.Pathogenic differentiation and AFLP analysis of the rice sheath blight strains from Heilongjiang.Plant Prot,2008,34(6):57-61.(in Chinese with English abstract)
[12]朱名海,皮磊,舒灿伟,周而勋.南繁区稻瘟病菌遗传多样性和群体遗传结构的AFLP分析.中国水稻科学,2017,31(3):320-326.Zhu M H,Pi L,Shu C W,Zhou E X.AFLP analyses of genetic diversity and population genetic structure of Magnaporthe oryzae from South China Crop Breeding Area.Chin J Rice Sci,2017,31(3):320-326.(in Chinese with English abstract)
[13]周而勋,杨媚.从植物病组织中分离丝核菌的快速、简便技术.华南农业大学学报,1998,19(1):125-126.Zhou E X,Yang M.A rapid and simple technique for the isolation of Rhizoctonia solani from diseased plant tissues.J South China Agric Univ,1998,19(1):125-126.(in Chinese with English abstract)
[14]黄江华,杨媚,周而勋,戚佩坤.丝核菌细胞核染色技术的研究.仲恺农业技术学院学报,2001,14(4):13-17.Huang J H,Yang M,Zhou E X,Qi P K.Studies on the nuclear staining techniques for Rhizoctonia spp.JZhongkai Agrotechnol Coll,2001,14(4):13-17.(in Chinese with English abstract)
[15]周而勋,杨媚.广东省水稻纹枯病菌的致病力和融合群研究.广东农业科学,1999,26(5):36-38.Zhou E X,Yang M.Studies on the pathogenicity and anastomosis group of Rhizoctonia solani in Guangdong province.Guangdong Agric Sci,1999,26(5):36-38.(in Chinese)
[16]Brant J B,Gustavo C A,Peter M G.Fast and sensitive silver staining of DNA in polyacrylamide gels.Anal Biochem,1991,196(1):80-83.
[17]陈涛,张震,柴荣耀,王教瑜,毛雪琴,邱海萍,杜新法,姜华,王立安,王艳丽,孙国昌.浙江省水稻纹枯病菌的遗传分化与致病力研究.中国水稻科学,2010,24(1):67-72.Chen T,Zhang Z,Chai R Y,Wang J Y,Mao X Q,Qiu HP,Du X F,Jiang H,Wang L A,Wang Y L,Sun G C.Genetic diversity and pathogenicity variation of different Rhizoctonia solani isolates in rice from Zhejiang Province,China.Chin J Rice Sci,2010,24(1):67-72.(in Chinese with English abstract)
[18]黄雯雯,王玲,刘连盟,刘恩勇,黄世文.安徽省水稻纹枯病菌致病力分化与遗传多样性.浙江农业学报,2011,23(1):111-116.Huang W W,Wang L,Liu L M,Liu E Y,Huang S W.Preliminary study on the pathogenic differentiation and genetic diversity of rice sheath blight pathogen strains from Anhui Province.Acta Agric Zhejiang,2011,23(1):111-116.(in Chinese with English abstract)
[19]吴荷芳,王晓宇,罗楚平,陈志谊.江苏省水稻纹枯病菌遗传多样性分析与致病力研究.江苏农业学报,201329(1):51-59.Wu H F,Wang X Y,Luo C P,Chen Z Y.Genetic diversity and pathogenicity of Rhizoctonia solani isolates from Jiangsu province.Jiangsu J Agric Sci,2013 29(1):51-59.(in Chinese with English abstract)
[20]张优,魏松红,王海宁,李帅,李思博,罗文芳.东北地区水稻纹枯病菌遗传多样性和致病性分析.沈阳农业大学学报,2017,48(1):9-14.Zhang Y,Wei S H,Wang H N,Li S,Li S B,Luo W F.Genetic diversity and pathogenicity of Rhizoctonia solani isolates causing sheath blight disease of rice in northeast region.J Shenyang Agric Univ,2017,48(1):9-14.(in Chinese with English abstract)
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