红豆杉枝叶干燥及贮存工艺研究
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摘要
研究了红豆杉(Taxus chinensis)鲜枝叶采收后的干燥、贮存方式及贮存期限对枝叶中有效产物10-DABⅢ和紫杉醇含量的影响,结果表明:(1)采用不同干燥方法处理时,红豆杉样品中10-DABⅢ、紫杉醇含量具有显著差异(P<0.05)。同一温度干燥时,干燥时间越长,样品中10-DABⅢ和紫杉醇含量下降越快;在一定温度范围内(约25～100℃)干燥时,干燥温度越高,则干燥时间越短。产物含量与枝叶干燥时间和温度呈负相关;(2)贮存期间发生霉变的红豆杉鲜枝叶和枝叶干粉其产物含量均会逐渐降低,且新鲜枝叶中产物含量降低较快;(3)贮存期间红豆杉枝叶中10-DABⅢ产物较紫杉醇不稳定,更易受环境条件影响而降解。(4)长期贮存的红豆杉枝叶的含水量应控制在10%以内,贮存环境应该阴凉、干燥,且贮存时间不宜超过1 a。
The impacts of drying methods, storage methods and storage time on the content of 10-DAB Ⅲ and paclitaxel, in the fresh branches and leaves of Taxus chinensis were studied. The results showed that:(1) The contents of 10-DAB Ⅲ and paclitaxel in Taxus chinensis samples treated by different drying methods were significantly different(P < 0.05). At the same temperature, the longer drying time meant the 10 DAB Ⅲ and praclitaxel losing more content in the sample. Under certain temperature(about 25 to 100 ℃), the drying time shortened when the temperature increased. And the effective product's content was negatively correlated with both the drying time and the temperature.(2) The content of the effective constituent gradually decreased after the fresh branches, leaves and theirs dry powder were mildewed. And the content of effective constituent in fresh branches and leaves decreased faster than in dry powder.(3) During storage time, 10-DABⅢ was more unstable than taxol, which was more susceptible to be degraded under the in?uence of environmental conditions.(4) The water content of T. chinensis should be controlled within 10% during in the long-term storage. The storage environment should be cool and dry,the storage time should not exceed one year.
The impacts of drying methods, storage methods and storage time on the content of 10-DAB Ⅲ and paclitaxel, in the fresh branches and leaves of Taxus chinensis were studied. The results showed that:(1) The contents of 10-DAB Ⅲ and paclitaxel in Taxus chinensis samples treated by different drying methods were significantly different(P < 0.05). At the same temperature, the longer drying time meant the 10 DAB Ⅲ and praclitaxel losing more content in the sample. Under certain temperature(about 25 to 100 ℃), the drying time shortened when the temperature increased. And the effective product's content was negatively correlated with both the drying time and the temperature.(2) The content of the effective constituent gradually decreased after the fresh branches, leaves and theirs dry powder were mildewed. And the content of effective constituent in fresh branches and leaves decreased faster than in dry powder.(3) During storage time, 10-DABⅢ was more unstable than taxol, which was more susceptible to be degraded under the in?uence of environmental conditions.(4) The water content of T. chinensis should be controlled within 10% during in the long-term storage. The storage environment should be cool and dry,the storage time should not exceed one year.
引文
[1]李志良,何伟强,肖腊兴,等.红豆杉育苗及造林技术[J].广东林业科技, 2011, 27(6):92-95.
[2]韩锐.紫杉醇药理活性研究进展[J].中国肿瘤临床, 1993,20(3):223-227.
[3]刘介东,刘善辉,陈建芬,等.南方红豆杉组织培养技术[J].广东林业科技, 2015, 31(3):132-134.
[4]翁开敏,贺全山.紫杉醇研究概况[J].海峡药学,2002,14(2):5-7.
[5]阎家麒.紫杉醇手性侧链合成与紫杉醇半合成评述[J].江苏化工, 2005, 33(6):46-51.
[6]吴楠,吕扬.紫杉类化合物研究进展[J].化学进展,1997,9(1):1-13.
[7]李耀辉.红豆杉紫杉烷类化合物超临界二氧化碳萃取及其抑瘤作用研究[J].陕西医学杂志, 2009, 38(4):426-427.
[8]薛艳华,祖元刚,史权,等.几种影响提取东北红豆杉鲜枝叶中紫杉醇含量的干燥方法比较[J].植物生理学通讯, 2005, 41(5):657-658.
[9]上官晓晴,张春椿,何佳奇,等.不同湿度贮藏条件下南方红豆杉紫杉烷类含量动态变化的研究[J].浙江中医药大学学报, 2016(5):334-338.
[10]何佳奇,郑敏霞,何晓其.干燥方法和储藏环境对南方红豆杉总多糖含量的影响[J].中国中医药科技, 2018,25(2):227-229.
[11]惠俊峰.从红豆杉中提取紫杉醇及10DAB的研究[D].西安:西北大学, 2006.
[12]黄旭梁,刘开生,徐垒柱.高效液相色谱法测定南方红豆杉中紫杉醇的含量[J].现代中药研究与实践, 2010,24(4):60-62.
[13]柯春婷,仝川,王玉震,等.不同地理种源南方红豆杉中紫杉醇和10-DAB含量及影响因子[J].生态学杂志,2009, 28(2):231-236.
[2]韩锐.紫杉醇药理活性研究进展[J].中国肿瘤临床, 1993,20(3):223-227.
[3]刘介东,刘善辉,陈建芬,等.南方红豆杉组织培养技术[J].广东林业科技, 2015, 31(3):132-134.
[4]翁开敏,贺全山.紫杉醇研究概况[J].海峡药学,2002,14(2):5-7.
[5]阎家麒.紫杉醇手性侧链合成与紫杉醇半合成评述[J].江苏化工, 2005, 33(6):46-51.
[6]吴楠,吕扬.紫杉类化合物研究进展[J].化学进展,1997,9(1):1-13.
[7]李耀辉.红豆杉紫杉烷类化合物超临界二氧化碳萃取及其抑瘤作用研究[J].陕西医学杂志, 2009, 38(4):426-427.
[8]薛艳华,祖元刚,史权,等.几种影响提取东北红豆杉鲜枝叶中紫杉醇含量的干燥方法比较[J].植物生理学通讯, 2005, 41(5):657-658.
[9]上官晓晴,张春椿,何佳奇,等.不同湿度贮藏条件下南方红豆杉紫杉烷类含量动态变化的研究[J].浙江中医药大学学报, 2016(5):334-338.
[10]何佳奇,郑敏霞,何晓其.干燥方法和储藏环境对南方红豆杉总多糖含量的影响[J].中国中医药科技, 2018,25(2):227-229.
[11]惠俊峰.从红豆杉中提取紫杉醇及10DAB的研究[D].西安:西北大学, 2006.
[12]黄旭梁,刘开生,徐垒柱.高效液相色谱法测定南方红豆杉中紫杉醇的含量[J].现代中药研究与实践, 2010,24(4):60-62.
[13]柯春婷,仝川,王玉震,等.不同地理种源南方红豆杉中紫杉醇和10-DAB含量及影响因子[J].生态学杂志,2009, 28(2):231-236.