湖南省稻水象甲的遗传多样性及入侵扩散特点
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摘要
为全面了解湖南省稻水象甲的入侵扩散特点,运用简单重复序列间区(ISSR)分子标记方法及对比COⅠ基因、ITS2片段的序列信息,探讨全省17个地理种群的遗传多样性和扩散模式。结果表明:湖南省稻水象甲种群COⅠ基因、ITS2片段的序列非常保守,不能提供有效的多态性位点信息,而ISSR的多态性信息非常丰富,其多态性位点百分率可达93.24%;基于ISSR的数据分析发现,湖南省稻水象甲的Nei’s基因多样性指数为0.363 0,各地理种群间基因分化系数为0.414 3,种群之间的遗传距离与地理距离无相关性。湖南省稻水象甲地理种群分化不符合地理隔离模式,以人为因素导致的扩散为主,自然扩散为辅。
To understand the geographical range expansion of Lissorhoptrus oryzophilus(L. oryzophilus), a rice weevil,in Hunan province, inter–simple sequence repeat(ISSR) molecular markers, mitochondrial COⅠgene and ITS2 sequence were examined to investigate the genetic diversity and expansion model of 17 geographic populations. The results showed that mitochondrial COⅠ gene and ITS2 sequence were both conservative in different individuals, which was inapropriate for effective polymophism analysis. However, the percentage of polymorphic bands of ISSR of populations reaches to 93.24%. After further analyzing, we found that Nei's gene diversity index(H) was 0.363 0, and the coefficient of genetic differentiation(Gst) among populations was 0.414 3. There was no significant correlation between genetic distance and geographical distance. The morphological differentiation of L. oryzophilus is not in conformity with the geographical isolation mode in Hunan province, indicating the rapid spread of rice water weevil in Hunan province was mainly related to artificial transport and less related to natural factors.
To understand the geographical range expansion of Lissorhoptrus oryzophilus(L. oryzophilus), a rice weevil,in Hunan province, inter–simple sequence repeat(ISSR) molecular markers, mitochondrial COⅠgene and ITS2 sequence were examined to investigate the genetic diversity and expansion model of 17 geographic populations. The results showed that mitochondrial COⅠ gene and ITS2 sequence were both conservative in different individuals, which was inapropriate for effective polymophism analysis. However, the percentage of polymorphic bands of ISSR of populations reaches to 93.24%. After further analyzing, we found that Nei's gene diversity index(H) was 0.363 0, and the coefficient of genetic differentiation(Gst) among populations was 0.414 3. There was no significant correlation between genetic distance and geographical distance. The morphological differentiation of L. oryzophilus is not in conformity with the geographical isolation mode in Hunan province, indicating the rapid spread of rice water weevil in Hunan province was mainly related to artificial transport and less related to natural factors.
引文
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[5]焦文娟.稻水象甲及常见田间昆虫体内Wolbachia的检测与序列分析[D].南昌:江西农业大学,2013.
[6]柳青,李燕,王德海,等.基于ISSR分子标记技术的稻水象甲遗传多样性研究[J].环境昆虫学报,2016,38(1):102–112.
[7]王小武,付开赟,丁新华,等.基于RAPD标记的新疆荒漠稻区稻水象甲遗传多样性分析[J].新疆农业科学,2016,53(11):2069–2076.
[8]张文俊.稻水象甲Lissorhoptrus oryzophilus Kuschel种群数量扩张机制的研究[D].杭州:浙江大学,2007.
[9]李晴,梁玉勇,厉建蕾,等.基于COⅠ基因的二化螟种群遗传多样性检测方法[J].应用昆虫学报,2014,51(5):1237–1245.
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[11]张国强.ISSR分子标记揭示黑斑侧褶蛙中国种群的遗传结构和遗传多样性[D].南京:南京师范大学,2005.
[12] GRIGARICK A A.Study of the rice water weevil,past,present,and future in the United States with emphasis on California[C]//HIRAI K.Establishment,Spread,and Management at the Rice Water Weevil and Migratory Rice Insect Pests in East Asia.Tsukuba:NARC,1993:12–31.
[13] TANAKA A. Ecology of the RWW, Lissorhoptrus oryzophilus Kuschel,in Kyushu and Okinawa,Japan[C]//HIRAI K.Establishment,Spread,and Management at the Rice Water Weevil and Migratory Rice Insect Pests in East Asia.Tsukuba:NARC,1993:191–213.
[14]赵冲.我国烟蚜种群分化的研究[D].北京:中国农业科学院,2014.
[15]张润杰,何新凤.昆虫生态地理学与入侵危险性害虫控制[J].生态科学,1997,16(1):83–87.
[16]张林艳,叶万辉,江洪.利用反应–扩散模型预测生物入侵[J].生态环境学报,2009,18(4):1565–1572.
[17]黄志农,刘勇,张玲,等.湖南稻水象甲疫情防控初报[J].湖南农业科学,2006(2):62–63.
[18]齐国君,高燕,黄德超,等.基于MAXENT的稻水象甲在中国的入侵扩散动态及适生性分析[J].植物保护学报,2012,39(2):129–136.
[19]孟维亮,于飞,敖芹,等.稻水象甲的入侵特征与适应性分析[J].耕作与栽培,2011(2):19–20.
[20]赵晓明,张增福,陆占军,等.稻水象甲传播扩散调查与实例分析[J].宁夏农林科技,2017,58(1):41–43.
[2] HESLER,LOUIS S,MICHAEL J,et al.Numbers of rice water weevil larvae(Coleoptera:Curculionidae)and rice plant growth in relation to adult infestation levels and broadleaf herbicide applications[J]. J Agric Urban Entomol,2000,17(2):99–108.
[3]全国农业技术推广服务中心.中国稻水象甲[M].北京:农业出版社,1988.
[4]朱赞江.湖南省双峰县稻区稻水象甲发生为害调查及防控对策[D].南宁:广西大学,2014.
[5]焦文娟.稻水象甲及常见田间昆虫体内Wolbachia的检测与序列分析[D].南昌:江西农业大学,2013.
[6]柳青,李燕,王德海,等.基于ISSR分子标记技术的稻水象甲遗传多样性研究[J].环境昆虫学报,2016,38(1):102–112.
[7]王小武,付开赟,丁新华,等.基于RAPD标记的新疆荒漠稻区稻水象甲遗传多样性分析[J].新疆农业科学,2016,53(11):2069–2076.
[8]张文俊.稻水象甲Lissorhoptrus oryzophilus Kuschel种群数量扩张机制的研究[D].杭州:浙江大学,2007.
[9]李晴,梁玉勇,厉建蕾,等.基于COⅠ基因的二化螟种群遗传多样性检测方法[J].应用昆虫学报,2014,51(5):1237–1245.
[10]张琰.基于ISSR分子标记的角倍蚜种群遗传多态性[D].太原:山西大学,2006.
[11]张国强.ISSR分子标记揭示黑斑侧褶蛙中国种群的遗传结构和遗传多样性[D].南京:南京师范大学,2005.
[12] GRIGARICK A A.Study of the rice water weevil,past,present,and future in the United States with emphasis on California[C]//HIRAI K.Establishment,Spread,and Management at the Rice Water Weevil and Migratory Rice Insect Pests in East Asia.Tsukuba:NARC,1993:12–31.
[13] TANAKA A. Ecology of the RWW, Lissorhoptrus oryzophilus Kuschel,in Kyushu and Okinawa,Japan[C]//HIRAI K.Establishment,Spread,and Management at the Rice Water Weevil and Migratory Rice Insect Pests in East Asia.Tsukuba:NARC,1993:191–213.
[14]赵冲.我国烟蚜种群分化的研究[D].北京:中国农业科学院,2014.
[15]张润杰,何新凤.昆虫生态地理学与入侵危险性害虫控制[J].生态科学,1997,16(1):83–87.
[16]张林艳,叶万辉,江洪.利用反应–扩散模型预测生物入侵[J].生态环境学报,2009,18(4):1565–1572.
[17]黄志农,刘勇,张玲,等.湖南稻水象甲疫情防控初报[J].湖南农业科学,2006(2):62–63.
[18]齐国君,高燕,黄德超,等.基于MAXENT的稻水象甲在中国的入侵扩散动态及适生性分析[J].植物保护学报,2012,39(2):129–136.
[19]孟维亮,于飞,敖芹,等.稻水象甲的入侵特征与适应性分析[J].耕作与栽培,2011(2):19–20.
[20]赵晓明,张增福,陆占军,等.稻水象甲传播扩散调查与实例分析[J].宁夏农林科技,2017,58(1):41–43.