中药茜草抗氧化、抗炎、抗肿瘤不同药用部位精准研究
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摘要
目的:研究和比较茜草根、茎、叶、花及果的抗氧化、抗炎、抗肿瘤活性,明确茜草发挥特定药效的部位,为茜草资源合理应用提供科学依据。方法:采用HPLC法建立茜草根、茎、叶、花、果的指纹图谱;采用二苯代苦味酰基自由基(DPPH)、2,2-连氮(3-乙基苯并噻唑啉-6磺酸)二氨盐自由基(ABTS)法,以半数清除浓度(EC50)评价茜草各部位醇提物抗氧化活性;采用脂多糖(LPS)诱导小鼠巨噬细胞RAW264.7细胞建立炎症模型,以NO、IL-6炎性因子评价茜草各部位抗炎活性;采用噻唑蓝法(MTT),以抑制率评价茜草各部位醇提物对人肝癌HepG2细胞增殖的影响。结果:茜草各部位HPLC指纹图谱在色谱峰数量及相对峰面积方面存在较大差异,通过化学对照品比对出12种化学成分;茜草根醇提物对肝肿瘤细胞的抑制作用最显著,抗炎效果最好,茜草花醇提物抗氧化活性最高。结论:茜草不同部位化学成分群存在差异,差异的化学成分群是茜草不同部位抗氧化、抗炎、抗肿瘤药效显著差异的重要因素,本实验为茜草各药用部位的进一步深入研究及临床合理应用提供参考。
To study and compare the antioxidant, anti-inflammatory and anti-tumor activities of different medicinal parts of Rubia cordifolia L. and identify the specific medicinal effects of them. The fingerprints of different parts of Rubia cordifolia L. were established by HPLC, the antioxidant activity of Rubia cordifolia L roots, stems, leaves, flowers, fruits were determined by DPPH and ABTS methods, whereafter the LPS was used to induce inflammation in RAW264.7 cells and inflammatory factors were used to evaluate anti-inflammatory effect. In the end, MTT method was used to determine the inhibition rate of hepatoma cells. The results showed that there were differences in fingerprints of different parts of Rubia cordifolia L., including the number and area of fingerprint peaks, which 12 chemical components were identified.The extracts of Rubia cordifolia L.roots had the most obvious inhibitory effect on hepatoma cells and the best antiinflammatory effect on RAW264.7 cells, while the extracts of Rubia cordifolia L flower had the highest antioxidant activity. The chemical composition difference in different parts of Rubia cordifolia L. led to the significant differences of different parts of Rubia cordifolia L. in antioxidant, anti-inflammatory and anti-tumor effects. This experiment provides a reference for further in-depth research and clinical rational application of Rubia cordifolia L.
To study and compare the antioxidant, anti-inflammatory and anti-tumor activities of different medicinal parts of Rubia cordifolia L. and identify the specific medicinal effects of them. The fingerprints of different parts of Rubia cordifolia L. were established by HPLC, the antioxidant activity of Rubia cordifolia L roots, stems, leaves, flowers, fruits were determined by DPPH and ABTS methods, whereafter the LPS was used to induce inflammation in RAW264.7 cells and inflammatory factors were used to evaluate anti-inflammatory effect. In the end, MTT method was used to determine the inhibition rate of hepatoma cells. The results showed that there were differences in fingerprints of different parts of Rubia cordifolia L., including the number and area of fingerprint peaks, which 12 chemical components were identified.The extracts of Rubia cordifolia L.roots had the most obvious inhibitory effect on hepatoma cells and the best antiinflammatory effect on RAW264.7 cells, while the extracts of Rubia cordifolia L flower had the highest antioxidant activity. The chemical composition difference in different parts of Rubia cordifolia L. led to the significant differences of different parts of Rubia cordifolia L. in antioxidant, anti-inflammatory and anti-tumor effects. This experiment provides a reference for further in-depth research and clinical rational application of Rubia cordifolia L.
引文
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2 李俊峰,舒建昌,杨钦河,等.大叶茜草素抑制大鼠CFSC-2G细胞活化和胶原合成.中国病理生理杂志, 2017, 33(12):2259-2263.
3 Manoharan R K, Lee J H, Kim Y G. Alizarin and chrysazin inhibit biofilm and hyphal formation by front cell infect microbiol. Frontiers in Cellular and Infection Microbiology, 2017(7):447.
4 Zengin G, Degirmenci N S, Alpsoy L. Evaluation of antioxidant, enzyme inhibition, and cytotoxic activity of three anthraquinones(alizarin,purpurin, and quinizarin). Human&Experimental Toxicology, 2016, 35(5):544-553.
5 Nam W, Kim S P, Nam S H, et al. Structure-antioxidative and antiinflammatory activity relationships of purpurin and related anthraquinones in chemical and cell assays. Molecules, 2017, 22(2):265.
6 Gong X P, Sun Y Y, Chen W, et al. Anti-diarrheal and antiinflammatory activities of aqueous extract of the aerial part of Rubia cordifolia. BMC Complementary and Alternative Medicine, 2017, 17(1):20.
7 田春莲,覃基信,彭贤俊.茜草根、茎、叶多糖含量分析.中国野生植物资源, 2004(3):50-51+65.
8 周琼,剌瑞哲,赵慧贤.茜草营养成分的研究.陕西农业科学, 2011,57(2):64-65.
9 侯显玉.儿泻停颗粒治疗小儿腹泻病的临床效果.临床医药文献电子杂志, 2014, 1(12):2134+2137.
10 张茂婷,杨柳,胡江苗,等.茜草地上部分木脂素类成分研究.中草药, 2017, 48(23):4856-4859.
11 曾维才,石碧.天然产物抗氧化活性的常见评价方法.化工进展,2013, 32(6):1205-1213+1247.
12 杨红红,符悦冠,肖烽,等.中药茜草根乙酸乙酯萃取物的分离及抗氧化活性研究.武汉工程大学学报, 2010, 32(12):1-5.
13 王会,周燕.筛选和表征抗氧化剂的方法-ABTS法.广州化工, 2012,40(22):41-43.
14 王金梅,姚辰,李昌勤,等.基于DPPH、ABTS和FRAP法的红花抗氧化谱效关系研究.中国药学杂志, 2017, 52(10):825-831.
15 刘瑞凝,王伟,谢伟东,等.雪白睡莲花中黄酮类成分抗氧化及拟缺血对神经胞作用的研究.世界科学技术-中医药现代化, 2006, 8(5):33-36.
16 田淑霞,李长艳,张婧娴,等.东北铁线莲不同提取部位的抗炎镇痛作用研究.世界中医药, 2018, 13(8):2019-2022.
17 单梅华,刘晓光,朱毓永,等.山杏叶水提物对脂多糖诱导的RAW264.7细胞的体外抗炎作用.北方园艺, 2018(18):132-137.
18 丛友权,韩晶晶,方芸,等.雪荔方总黄酮对LPS致RAW264.7细胞炎症的保护作用.中成药, 2017, 39(5):890-895.
19 李慧,孟宪生.中药茜草的本草考证.中药材, 2018, 41(10):2212-2216.
20 张茂婷,张虹,张耀如,等.茜草地上部分的化学成分研究.安徽中医药大学学报, 2017, 36(3):82-85.
21 樊佳新,包永睿,孟宪生,等.基于微流控芯片技术的荆芥不同药用部位抗肺肿瘤药效关系研究.中国中药杂志, 2017, 42(9):1717-1721.
22 倪翀,郭世昌,杜琰,等.饮食营养与炎症-癌症危险性的关系.第二军医大学学报, 2015, 36(10):1117-1122.
23 刘朝阳.自由基与癌症研究.医学研究讯, 1995(3):13-15.
2 李俊峰,舒建昌,杨钦河,等.大叶茜草素抑制大鼠CFSC-2G细胞活化和胶原合成.中国病理生理杂志, 2017, 33(12):2259-2263.
3 Manoharan R K, Lee J H, Kim Y G. Alizarin and chrysazin inhibit biofilm and hyphal formation by front cell infect microbiol. Frontiers in Cellular and Infection Microbiology, 2017(7):447.
4 Zengin G, Degirmenci N S, Alpsoy L. Evaluation of antioxidant, enzyme inhibition, and cytotoxic activity of three anthraquinones(alizarin,purpurin, and quinizarin). Human&Experimental Toxicology, 2016, 35(5):544-553.
5 Nam W, Kim S P, Nam S H, et al. Structure-antioxidative and antiinflammatory activity relationships of purpurin and related anthraquinones in chemical and cell assays. Molecules, 2017, 22(2):265.
6 Gong X P, Sun Y Y, Chen W, et al. Anti-diarrheal and antiinflammatory activities of aqueous extract of the aerial part of Rubia cordifolia. BMC Complementary and Alternative Medicine, 2017, 17(1):20.
7 田春莲,覃基信,彭贤俊.茜草根、茎、叶多糖含量分析.中国野生植物资源, 2004(3):50-51+65.
8 周琼,剌瑞哲,赵慧贤.茜草营养成分的研究.陕西农业科学, 2011,57(2):64-65.
9 侯显玉.儿泻停颗粒治疗小儿腹泻病的临床效果.临床医药文献电子杂志, 2014, 1(12):2134+2137.
10 张茂婷,杨柳,胡江苗,等.茜草地上部分木脂素类成分研究.中草药, 2017, 48(23):4856-4859.
11 曾维才,石碧.天然产物抗氧化活性的常见评价方法.化工进展,2013, 32(6):1205-1213+1247.
12 杨红红,符悦冠,肖烽,等.中药茜草根乙酸乙酯萃取物的分离及抗氧化活性研究.武汉工程大学学报, 2010, 32(12):1-5.
13 王会,周燕.筛选和表征抗氧化剂的方法-ABTS法.广州化工, 2012,40(22):41-43.
14 王金梅,姚辰,李昌勤,等.基于DPPH、ABTS和FRAP法的红花抗氧化谱效关系研究.中国药学杂志, 2017, 52(10):825-831.
15 刘瑞凝,王伟,谢伟东,等.雪白睡莲花中黄酮类成分抗氧化及拟缺血对神经胞作用的研究.世界科学技术-中医药现代化, 2006, 8(5):33-36.
16 田淑霞,李长艳,张婧娴,等.东北铁线莲不同提取部位的抗炎镇痛作用研究.世界中医药, 2018, 13(8):2019-2022.
17 单梅华,刘晓光,朱毓永,等.山杏叶水提物对脂多糖诱导的RAW264.7细胞的体外抗炎作用.北方园艺, 2018(18):132-137.
18 丛友权,韩晶晶,方芸,等.雪荔方总黄酮对LPS致RAW264.7细胞炎症的保护作用.中成药, 2017, 39(5):890-895.
19 李慧,孟宪生.中药茜草的本草考证.中药材, 2018, 41(10):2212-2216.
20 张茂婷,张虹,张耀如,等.茜草地上部分的化学成分研究.安徽中医药大学学报, 2017, 36(3):82-85.
21 樊佳新,包永睿,孟宪生,等.基于微流控芯片技术的荆芥不同药用部位抗肺肿瘤药效关系研究.中国中药杂志, 2017, 42(9):1717-1721.
22 倪翀,郭世昌,杜琰,等.饮食营养与炎症-癌症危险性的关系.第二军医大学学报, 2015, 36(10):1117-1122.
23 刘朝阳.自由基与癌症研究.医学研究讯, 1995(3):13-15.