鹿角帽水溶性提取物双相调节乳腺癌细胞增殖
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摘要
初步探讨SAB抑制乳腺癌增殖的剂量特征和机制.采用MTT法检测不同浓度SAB对人乳腺癌细胞MCF-7增殖的影响,进一步利用RT-PCR、Western blot等方法观察细胞周期蛋白、细胞凋亡相关因子、ER-α36、ER-α66等表达的变化.结果显示,低剂量(0.25mg/mL)的SAB促进MCF-7细胞的增殖,高剂量的SAB才能抑制MCF-7细胞的增殖,促进细胞凋亡,并且将细胞周期阻滞在S期.提示SAB通过影响不同ER亚型的表达介导不同的ER信号通路,从而起到对乳腺癌细胞的双相调节作用.
To investigate the dose characteristics and mechanism of SAB inhibition of breast cancer proliferation.In this paper,the effects of different concentrations of SAB on the proliferation of human breast cancer cell line MCF-7 were detected by MTT assay.The expressions of Cyclin,apoptosis-related factors,ER-α36 and ER-α66 were further observed by RT-PCR,Western blot.The dose characteristics and mechanism of SAB inhibition of breast cancer proliferation were preliminarily investigated.It was found that low dose(0.25 mg/mL)of SAB promoted the proliferation of MCF-7 cells,and high doses of SAB inhibited the proliferation of MCF-7 cells,promoted apoptosis,arrested the cell cycle in S phase.It is suggested that SAB may mediate different ER signaling pathways by affecting the expression of different ER subtypes,thereby regulating the biphasic function of breast cancer cells.
To investigate the dose characteristics and mechanism of SAB inhibition of breast cancer proliferation.In this paper,the effects of different concentrations of SAB on the proliferation of human breast cancer cell line MCF-7 were detected by MTT assay.The expressions of Cyclin,apoptosis-related factors,ER-α36 and ER-α66 were further observed by RT-PCR,Western blot.The dose characteristics and mechanism of SAB inhibition of breast cancer proliferation were preliminarily investigated.It was found that low dose(0.25 mg/mL)of SAB promoted the proliferation of MCF-7 cells,and high doses of SAB inhibited the proliferation of MCF-7 cells,promoted apoptosis,arrested the cell cycle in S phase.It is suggested that SAB may mediate different ER signaling pathways by affecting the expression of different ER subtypes,thereby regulating the biphasic function of breast cancer cells.
引文
[1]JIANG W,YIN Y G,ZHOU Y J,et al.Isolation and characterization of peptidoglycan recognition protein 1from antler base of sika deer(Cervus nippon)[J].Int J Biol Macromol,2014,64:313-318.
[2]WU F F,LI H Q,JIN L J,et al.Deer antler base as a traditional Chinese medicine:A review of its traditional uses,chemistry and pharmacology[J].J Ethnopharmacol,2013,145(2):403-415.
[3]赵文静,赵向上,旺建伟,等.复方鹿花盘胶囊对乳腺增生小鼠子宫、卵巢重量影响及作用机制的研究[J].中医药信息,2010(3):41-43.
[4]张雷,林艳.鹿角盘胶囊治疗乳腺增生症120例分析[J].中国误诊学杂志,2010(4):900-901.
[5]王志兵,邱芳萍,李治民,等.鹿角盘活性成分对小鼠吞噬功能及对大鼠乳腺增生激素水平的影响[J].食品科技,2007(11):225-226.
[6]李卫民,李廷冠,李敏江,等.乳腺康胶囊对乳腺增生大鼠乳腺组织和性激素的影响[J].中成药,2003,25(10):817-819.
[7]SHI L,DONG B,LI Z W,et al.Expression of ER-α36,a novel variant of estrogen receptorα,and resistance to tamoxifen treatment in breast cancer[J].J Clin Oncol,2009,27(21):3423-3429.
[8]WANG Z Y,YIN L.Estrogen receptor alpha-36(ER-α36):A new player in human breast cancer[J].Mol Cell Endocrinol,2015,418:193-206.
[9]WANG L M,XIE K P,HUO H N,et al.Luteolin inhibits proliferation induced by IGF-1pathway dependent ERαin human breast cancer MCF-7cells[J].Asian Pac J Cancer P,2012,13(4):1431-1437.
[10]刘洋,方晨,邹萍,等.新型雌激素受体ER-α36在新生与成年大鼠海马和皮层中表达的比较[J].生理学报,2013,65(3):263-268.
[11]CHEN W Q,ZHENG R S,BAADE P D,et al.Cancer statistics in China,2015[J].CA Cancer J Clin,2016,66(2):115-132.
[12]权石范,张秀莲,常忠娟.梅花鹿三种茸片和鹿角盘性激素含量测定[J].特产研究,2014,36(1):10-11.
[13]SUI J Q,XIE K P,ZOU W,et al.Emodin inhibits breast cancer cell proliferation through the ERα-MAPK/Akt-cyclin D1/Bcl-2signaling pathway[J].Asian Pac J Cancer Prev,2014,15(15):6247-6251.
[14]HU W,QI L,TIAN Y H,et al.Studies on the purification of polypeptide from sika antler plate and activities of antitumor[J].BMC Complement Altern Med,2015,15:328-336.
[15]王竞伟,邵先茹,段秀庆.Cyclin D1与乳腺癌的相关研究[J].医学综述,2018,24(4):677-681.
[16]WANG Z Y,ZHANG X T,SHEN P,et al.Identification,cloning,and expression of human estrogen receptor-a36,a novel variant of human estrogen receptor-α66[J].Biochem Bioph Res Co,2005,336(4):1023-1027.
[17]ZOU W,MCDANELD L,SMITH L M.Caveolin-1haploinsufficiency leads to partial transformation of human breast epithelial cells[J].Anticancer Res,2003,23(6C):4581-4586.
[18]LI G L,ZHANG J,JIN K T,et al.Estrogen receptor-α36is involved in development of acquired tamoxifen resistance via regulating the growth status switch in breast cancer cells[J].Mol Oncol,2013,7(3):611-624.
[2]WU F F,LI H Q,JIN L J,et al.Deer antler base as a traditional Chinese medicine:A review of its traditional uses,chemistry and pharmacology[J].J Ethnopharmacol,2013,145(2):403-415.
[3]赵文静,赵向上,旺建伟,等.复方鹿花盘胶囊对乳腺增生小鼠子宫、卵巢重量影响及作用机制的研究[J].中医药信息,2010(3):41-43.
[4]张雷,林艳.鹿角盘胶囊治疗乳腺增生症120例分析[J].中国误诊学杂志,2010(4):900-901.
[5]王志兵,邱芳萍,李治民,等.鹿角盘活性成分对小鼠吞噬功能及对大鼠乳腺增生激素水平的影响[J].食品科技,2007(11):225-226.
[6]李卫民,李廷冠,李敏江,等.乳腺康胶囊对乳腺增生大鼠乳腺组织和性激素的影响[J].中成药,2003,25(10):817-819.
[7]SHI L,DONG B,LI Z W,et al.Expression of ER-α36,a novel variant of estrogen receptorα,and resistance to tamoxifen treatment in breast cancer[J].J Clin Oncol,2009,27(21):3423-3429.
[8]WANG Z Y,YIN L.Estrogen receptor alpha-36(ER-α36):A new player in human breast cancer[J].Mol Cell Endocrinol,2015,418:193-206.
[9]WANG L M,XIE K P,HUO H N,et al.Luteolin inhibits proliferation induced by IGF-1pathway dependent ERαin human breast cancer MCF-7cells[J].Asian Pac J Cancer P,2012,13(4):1431-1437.
[10]刘洋,方晨,邹萍,等.新型雌激素受体ER-α36在新生与成年大鼠海马和皮层中表达的比较[J].生理学报,2013,65(3):263-268.
[11]CHEN W Q,ZHENG R S,BAADE P D,et al.Cancer statistics in China,2015[J].CA Cancer J Clin,2016,66(2):115-132.
[12]权石范,张秀莲,常忠娟.梅花鹿三种茸片和鹿角盘性激素含量测定[J].特产研究,2014,36(1):10-11.
[13]SUI J Q,XIE K P,ZOU W,et al.Emodin inhibits breast cancer cell proliferation through the ERα-MAPK/Akt-cyclin D1/Bcl-2signaling pathway[J].Asian Pac J Cancer Prev,2014,15(15):6247-6251.
[14]HU W,QI L,TIAN Y H,et al.Studies on the purification of polypeptide from sika antler plate and activities of antitumor[J].BMC Complement Altern Med,2015,15:328-336.
[15]王竞伟,邵先茹,段秀庆.Cyclin D1与乳腺癌的相关研究[J].医学综述,2018,24(4):677-681.
[16]WANG Z Y,ZHANG X T,SHEN P,et al.Identification,cloning,and expression of human estrogen receptor-a36,a novel variant of human estrogen receptor-α66[J].Biochem Bioph Res Co,2005,336(4):1023-1027.
[17]ZOU W,MCDANELD L,SMITH L M.Caveolin-1haploinsufficiency leads to partial transformation of human breast epithelial cells[J].Anticancer Res,2003,23(6C):4581-4586.
[18]LI G L,ZHANG J,JIN K T,et al.Estrogen receptor-α36is involved in development of acquired tamoxifen resistance via regulating the growth status switch in breast cancer cells[J].Mol Oncol,2013,7(3):611-624.
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