120份小麦品种(系)重要性状功能基因的KASP检测
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摘要
为了解120份小麦品种(系)重要性状功能基因的组成,本研究利用高通量的KASP标记技术对株高、抗叶锈病、抗条锈病、抗赤霉病、抗穗发芽和加工品质等性状相关的基因进行了检测。结果表明,供试材料的株高基因组成包含5种类型,分别是Rht-B1a/Rht-D1a (1份)、Rht-B1a/Rht-D1b (58份)、Rht-B1a+160/Rht-D1a (2份)、Rht-B1a+160/Rht-D1b (30份)和Rht-D1a/Rht-B1b (29份)。在抗病性方面,有69份材料含抗叶锈病基因Lr14a,3份材料含Lr68基因;抗叶锈病基因Lr21和Lr34、抗赤霉病基因Fhb1、抗条锈病基因Yr15在供试材料中均未发现。在抗穗发芽方面,68份材料为TaMFT-A1基因的Jagger-type抗穗发芽单倍型,21份材料为TaMoc-A1基因的Hap-H抗穗发芽单倍型,22份材料为TaSdr-D1基因的TaSdr-D1a抗穗发芽单倍型,84份材料为TaPHS1基因的Rio Blanco type抗穗发芽单倍型。在品质方面,83份材料为非1B/1R易位系,20份材料含高分子量麦谷蛋白亚基5+10,供试材料均不含Bx7OE。上述结果表明,对常规育种手段育成的高代品系进行KASP标记辅助选择可作为一种重要的分子育种策略,能显著地提高小麦育种效率。
In order to understand the composition of functional genes of the important traits in 120 wheat cultivars(lines), high-throughput KASP assays was used to detect some traits-associated genes related to plant height, leaf rust resistance, stripe rust resistance, fusarium head blight resistance, pre-harvest sprouting resistance and processing quality. The results showed that the plant height gene of the tested materials contained five types, which were Rht-B1 a/Rht-D1 a(1), Rht-B1 a/Rht-D1 b(58), Rht-B1 a +160/Rht-D1 a(2), Rht-B1 a +160/Rht-D1 b(30) and Rht-D1 a/Rht-B1 b(29), respectively. On disease resistance, 69 cultivars(lines) contained leaf rust resistance gene Lr14 a, and 3 cultivars(lines) contained Lr68 gene. The leaf rust resistance gene Lr21 and Lr34, fusarium head blight resistance gene Fhb1 and stripe rust resistance gene Yr15 were all not found. On the characteristics of pre-harvest sprouting resistance, 68 cultivars(lines) were Jagger-type haplotype of TaMFT-A1 gene, 21 cultivars(lines) were Hap-H haplotype of TaMoc-A1 gene, 22 cultivars(lines) were TaSdr-D1 a haplotype of TaSdr-D1 gene, and 84 cultivars(lines) were Rio Blanco haplotype of TaPHS1 gene. On quality, 83 cultivars(lines) were non1 B/1 R translocation lines, and 20 cultivars(lines) contained high molecular weight glutenin subunit 5+10, and Bx7 OE were not found in all materials. Above results showed that KASP marker assisted selection of advanced lines bred by conventional breeding could be an important molecular breeding strategy, which can significantly improve the efficiency of wheat breeding.
In order to understand the composition of functional genes of the important traits in 120 wheat cultivars(lines), high-throughput KASP assays was used to detect some traits-associated genes related to plant height, leaf rust resistance, stripe rust resistance, fusarium head blight resistance, pre-harvest sprouting resistance and processing quality. The results showed that the plant height gene of the tested materials contained five types, which were Rht-B1 a/Rht-D1 a(1), Rht-B1 a/Rht-D1 b(58), Rht-B1 a +160/Rht-D1 a(2), Rht-B1 a +160/Rht-D1 b(30) and Rht-D1 a/Rht-B1 b(29), respectively. On disease resistance, 69 cultivars(lines) contained leaf rust resistance gene Lr14 a, and 3 cultivars(lines) contained Lr68 gene. The leaf rust resistance gene Lr21 and Lr34, fusarium head blight resistance gene Fhb1 and stripe rust resistance gene Yr15 were all not found. On the characteristics of pre-harvest sprouting resistance, 68 cultivars(lines) were Jagger-type haplotype of TaMFT-A1 gene, 21 cultivars(lines) were Hap-H haplotype of TaMoc-A1 gene, 22 cultivars(lines) were TaSdr-D1 a haplotype of TaSdr-D1 gene, and 84 cultivars(lines) were Rio Blanco haplotype of TaPHS1 gene. On quality, 83 cultivars(lines) were non1 B/1 R translocation lines, and 20 cultivars(lines) contained high molecular weight glutenin subunit 5+10, and Bx7 OE were not found in all materials. Above results showed that KASP marker assisted selection of advanced lines bred by conventional breeding could be an important molecular breeding strategy, which can significantly improve the efficiency of wheat breeding.
引文
Cavanagh C.R.,Chao S.M.,Wang S.C.,Huang B.E.,Stephen S.,Kiani S.,Forrest K.,Saintenac C.,Brown-Guedira G.L.,Akhunova A.,See D.,Bai G.H.,Pumphrey M.,Tomar L.,Wong D.B.,Kong S.,Reynolds M.,da Silva M.L.,Bockelman H.,Talbert L.,Anderson J.A.,Dreisigacker S.,Baenziger S.,Carter A.,Korzun V.,Morrell P.L.,Dubcovsky J.,Morell M.K.,Sorrells M.E.,Hayden M.J.,and Akhunov E.,2013,Genome-wide comparative diversity uncovers multiple targets of selection for improvement in hexaploid wheat landraces and cultivars,Proc.Natl.Acad.Sci.USA,110(20):8057-8062
Cheng S.H.,Zhang Y.,Zhang B.Q.,Gao D.R.,Wu H.Y.,Lu C.B.,Lv G.F.,and Wang C.S.,2003,Discussion of two ways of breeding scab resistance in wheat,Yangzhou Daxue Xuebao(Journal of Yangzhou University(Agricultural and Life Sciences Edition)),24(1):59-62(程顺和,张勇,张伯桥,高德荣,吴宏亚,陆成彬,吕国锋,王朝顺,2003,小麦抗赤霉病育种2条技术路线的探讨,扬州大学学报(农业与生命科学版),24(1):59-62)
Ge R.J.,Liu T.G.,Gao L.,Liu B.,and Chen W.Q.,2015,Virulence of Puccinia triticina from 6 provinces in China in2011-2012,Zhiwu Bingli Xuebao(Acta Phytopathologica Sinica),45(2):175-180(葛润静,刘太国,高利,刘博,陈万权,2015,2011-2012年度中国六省小麦叶锈菌群体毒性分析,植物病理学报,45(2):175-180)
He Z.H.,Yan Y.M.,Zhuang Q.S.,Zhang Y.,Xia X.C.,Zhang Y.,Wang D.S.,Xia L.Q.,Hu Y.K.,Cai M.H.,Chen X.M.,Yan J.,and Zhou Y.,2006,Establishment of quality evaluation system and utilization of molecular methods for the improvement Chinese wheat quality,Zhongguo Nongye Kexue(Scientia Agriculturae Sinica),39(6):1091-1101(何中虎,晏月明,庄巧生,张艳,夏先春,张勇,王德森,夏兰芹,胡英考,蔡民华,陈新民,闫俊,周阳,2006,中国小麦品种品质评价体系建立与分子改良技术研究,中国农业科学,39(6):1091-1101)
Hedden P.,2003,The genes of the green revolution,Trends in Genetics,19(1):5-9
Herrera-Foessel S.A.,Singh R.P.,Huerta-Espino J.,Rosewarne G.M.,Periyannan S.K.,Viccars L.,Calvo-Salazar V.,Lan C.,and Lagudah E.S.,2012,Lr68:a new gene conferring slow rusting resistance to leaf rust in wheat,Theor.Appl.Genet.,124(8):1475-1486
Huang L.,and Gill B.S.,2001,An RGA-like marker detects all known Lr21 leaf rust resistance gene family members in Aegilops tauschii and wheat,Theor.Appl.Genet.,103(6-7):1007-1013
Lei L.,Zhu X.K.,Wang S.W.,Zhu M.R.,Carver B.F.,and Yan L.L.,2013,TaMFT-A1 is associated with seed germination sensitive to temperature in winter wheat,PLoS One,8(9):e73330
Li Y.,Wang J.K.,Qiu L.J.,Ma Y.Z.,Li X.H.,and Wan J.M.,2010,Crop molecular breeding in China:current status and perspectives,Zuowu Xuebao(Acta Agronomica Sinica),36(9):1425-1430(黎裕,王建康,邱丽娟,马有志,李新海,万建民,2010,中国作物分子育种现状与发展前景,作物学报,36(9):1425-1430)
Li Z.S.,2010,Retrospect and prospect of wheat breeding in China,Zhongguo Nongye Keji Daobao(Journal of Agricultural Science and Technology),12(2):1-4(李振声,2010,我国小麦育种的回顾与展望,中国农业科技导报,12(2):1-4)
Liu S.B.,Sehgal S.K.,Li J.R.,Lin M.,Trick H.N.,Yu J.M.,Gill B.S.,and Bai G.H.,2013,Cloning and characterization of a critical regulator for pre-harvest sprouting in wheat,Genetics,195(1):263-273
Rasheed A.,Wen W.E.,Gao F.M.,Zhai S.N.,Jin H.,Liu J.D.,Guo Q.,Zhang Y.J.,Dresigacker S.,Xia X.C.,and He Z.H.,2016,Development and validation of KASP assays for genes underpinning key economic traits in bread wheat,Theor.Appl.Genet.,129(10):1843-1860
Semagn K.,Babu R.,Hearne S.,and Olsen M.,2014,Single nucleotide polymorphism genotyping using kompetitive allele specific PCR(KASP):overview of the technology and its application in crop improvement,Mol.Breed.,33(1):1-14
Spielmeyer W.,Singh R.P.,McFadden H.,Wellings C.R.,Huerta-Espino J.,Kong X.,Appels R.,and Lagudah E.S.,2008,Fine scale genetic and physical mapping using interstitial deletion mutants of Lr34/Yr18:a disease resistance locus effective against multiple p/thogens in wheat,Theor.Appl.Genet.,116(4):481-490
Terracciano I.,Maccaferri M.,Bassi F.,Mantovani P.,Sanguineti M.C.,Salvi S.,Simkova H.,Dolezel J.,Massi A.,Ammar K.,Kolmer J.,and Tuberosa R.,2013,Development of COS-SNP and HRM markers for high-throughput and reliable haplotype-based detection of Lr14a in durum wheat(Triticum durum Desf.),Theor.Appl.Genet.,126(4):1077-1101
Tyrka M.,and Chelkowski J.,2004,Enhancing the resistance of triticale by using genes from wheat and rye,J.Appl.Genet.,45(3):283-295
Wang S.C.,Wong D.,Forrest K.,Allen A.,Chao S.M.,Huang B.E.,Maccaferri M.,Salvi S.,Milner S.G.,Cattivelli L.,Mastrangelo A.M.,Whan A.,Stephen S.,Barker G.,Wieseke R.,Plieske J.,Lillemo M.,Mather D.,Appels R.,Dolferus R.,Brown-Guedira G.,Korol A.,Akhunova A.R.,Feuillet C.,Salse J.,Morgante M.,Pozniak C.,Luo M.C.,Dvorak J.,Morell M.,Dubcovsky J.,Ganal M.,Tuberosa R.,Lawley C.,Mikoulitch I.,Cavanagh C.,Edwards K.J.,Hayden M.,and Akhunov E.,2014,Characterization of polyploid wheat genomic diversity using a high-density 90 000 single nucleotide polymorphism array,Plant Biotechnol.J.,12(6):787-796
Wang S.H.,Gong K.Y.,Chu B.Y.,Sun Q.Y.,and Ma Z.H.,2018,Molecular detection of strip rust resistance gene(s)in 100wheat cultivars(lines)from Sichuan province in China,Zhiwu Bingli Xuebao(Acta Phytopathologica Sinica),48(2):195-206(王树和,龚凯悦,初炳瑶,孙秋玉,马占鸿,2018,四川省100个小麦品种(系)抗条锈病基因的分子检测,植物病理学报,48(2):195-206)
Wilhelm E.P.,Mackay I.J.,Saville R.J.,Korolev A.V.,Balfourier F.,Greenland A.J.,Boulton M.I.,and Powell W.,2013,Haplotype dictionary for the Rht-1 loci in wheat,Theor.Appl.Genet.,126(7):1733-1747
Xiao S.H.,Zhang X.Y.,Yan C.S.,and Lin H.,2002,Germplasm improvement for pre-harvest sprouting resistance in Chinese white-grained wheat:an overview of the current in strategy,Euphytica,126(1):35-38
Zhang D.Q.,Song X.M.,Feng J.,Ma W.J.,Wu B.J.,Zhang C.L.,Cui Z.X.,Feng Y.,and Sun D.J.,2016,Detection of dwarf genes Rht-B1b,Rht-D1b and Rht8 in Huang-huai valley winter wheat areas and their influences on agronomic characteristics,Mailei Zuowu Xuebao(Journal of Triticeae Crops),36(8):975-981(张德强,宋晓朋,冯洁,马文洁,武炳瑾,张传量,崔紫霞,冯毅,孙道杰,2016,黄淮麦区小麦品种矮秆基因Rht-B1b,Rht-D1b和Rht8的检测及其对农艺性状的影响,麦类作物学报,36(8):975-981)
Zhang H.J.,Su Z.Q.,Bai G.H.,Zhang X.,Ma H.X.,Li T.,Deng Y.,Mai C.Y.,Yu L.Q.,Liu H.W.,Yang L.,Li H.J.,and Zhou Y.,2018,Improvement of resistance of wheat cultivars to Fusaruum head blight in the Yellow-Huai rivers valley winer wheat zone with functional marker selection of Fhb1gene,Zuowu Xuebao(Acta Agronomica Sinica),44(4):505-511(张宏军,宿振起,柏贵华,张旭,马鸿翔,李腾,邓云,买春艳,于立强,刘宏伟,杨丽,李洪杰,周阳,2018,利用Fhb1基因功能标记选择提高黄淮冬麦区小麦品种对赤霉病的抗性,作物学报,44(4):505-511)
Zhang X.K.,Yang S.J.,Zhou Y.,He Z.H.,and Xia X.C.,2006,Distribution of the Rht-B1b,Rht-D1b and Rht8 reduced height genes in autumn-sown Chinese wheats detected by molecular markers,Euphytica,152(1):109-116
Zhang Y.B.,Sun L.F.,Xin W.L.,Song Q.J.,Zhang C.L.,Zhao H.B.,Xiao Z.M.,and Qi S.Y.,2003,Effect of HMW-GS 5+10on quality improvement in some leading wheat cultivars,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),36(3):242-247(张延滨,孙连发,辛文利,宋庆杰,张春利,赵海滨,肖志敏,祁适雨,2003,主栽小麦品种中5+10亚基对品质改良的影响,中国农业科学,36(3):242-247)
Zhou Y.,He Z.H.,Zhang G.S.,Xia L.Q.,Chen X.M.,Gao Y.C.,Jing Z.B.,and Yu G.J.,2004,Utilization of 1BL/1RS translocation in wheat breeding in China,Zuowu Xuebao(Acta Agronomica Sinica),30(6):531-535(周阳,何中虎,张改生,夏兰琴,陈新民,高永超,井赵斌,于广军,2004,1BL/IRS易位系在中国小麦育种中的应用,作物学报,30(6):531-535)
Cheng S.H.,Zhang Y.,Zhang B.Q.,Gao D.R.,Wu H.Y.,Lu C.B.,Lv G.F.,and Wang C.S.,2003,Discussion of two ways of breeding scab resistance in wheat,Yangzhou Daxue Xuebao(Journal of Yangzhou University(Agricultural and Life Sciences Edition)),24(1):59-62(程顺和,张勇,张伯桥,高德荣,吴宏亚,陆成彬,吕国锋,王朝顺,2003,小麦抗赤霉病育种2条技术路线的探讨,扬州大学学报(农业与生命科学版),24(1):59-62)
Ge R.J.,Liu T.G.,Gao L.,Liu B.,and Chen W.Q.,2015,Virulence of Puccinia triticina from 6 provinces in China in2011-2012,Zhiwu Bingli Xuebao(Acta Phytopathologica Sinica),45(2):175-180(葛润静,刘太国,高利,刘博,陈万权,2015,2011-2012年度中国六省小麦叶锈菌群体毒性分析,植物病理学报,45(2):175-180)
He Z.H.,Yan Y.M.,Zhuang Q.S.,Zhang Y.,Xia X.C.,Zhang Y.,Wang D.S.,Xia L.Q.,Hu Y.K.,Cai M.H.,Chen X.M.,Yan J.,and Zhou Y.,2006,Establishment of quality evaluation system and utilization of molecular methods for the improvement Chinese wheat quality,Zhongguo Nongye Kexue(Scientia Agriculturae Sinica),39(6):1091-1101(何中虎,晏月明,庄巧生,张艳,夏先春,张勇,王德森,夏兰芹,胡英考,蔡民华,陈新民,闫俊,周阳,2006,中国小麦品种品质评价体系建立与分子改良技术研究,中国农业科学,39(6):1091-1101)
Hedden P.,2003,The genes of the green revolution,Trends in Genetics,19(1):5-9
Herrera-Foessel S.A.,Singh R.P.,Huerta-Espino J.,Rosewarne G.M.,Periyannan S.K.,Viccars L.,Calvo-Salazar V.,Lan C.,and Lagudah E.S.,2012,Lr68:a new gene conferring slow rusting resistance to leaf rust in wheat,Theor.Appl.Genet.,124(8):1475-1486
Huang L.,and Gill B.S.,2001,An RGA-like marker detects all known Lr21 leaf rust resistance gene family members in Aegilops tauschii and wheat,Theor.Appl.Genet.,103(6-7):1007-1013
Lei L.,Zhu X.K.,Wang S.W.,Zhu M.R.,Carver B.F.,and Yan L.L.,2013,TaMFT-A1 is associated with seed germination sensitive to temperature in winter wheat,PLoS One,8(9):e73330
Li Y.,Wang J.K.,Qiu L.J.,Ma Y.Z.,Li X.H.,and Wan J.M.,2010,Crop molecular breeding in China:current status and perspectives,Zuowu Xuebao(Acta Agronomica Sinica),36(9):1425-1430(黎裕,王建康,邱丽娟,马有志,李新海,万建民,2010,中国作物分子育种现状与发展前景,作物学报,36(9):1425-1430)
Li Z.S.,2010,Retrospect and prospect of wheat breeding in China,Zhongguo Nongye Keji Daobao(Journal of Agricultural Science and Technology),12(2):1-4(李振声,2010,我国小麦育种的回顾与展望,中国农业科技导报,12(2):1-4)
Liu S.B.,Sehgal S.K.,Li J.R.,Lin M.,Trick H.N.,Yu J.M.,Gill B.S.,and Bai G.H.,2013,Cloning and characterization of a critical regulator for pre-harvest sprouting in wheat,Genetics,195(1):263-273
Rasheed A.,Wen W.E.,Gao F.M.,Zhai S.N.,Jin H.,Liu J.D.,Guo Q.,Zhang Y.J.,Dresigacker S.,Xia X.C.,and He Z.H.,2016,Development and validation of KASP assays for genes underpinning key economic traits in bread wheat,Theor.Appl.Genet.,129(10):1843-1860
Semagn K.,Babu R.,Hearne S.,and Olsen M.,2014,Single nucleotide polymorphism genotyping using kompetitive allele specific PCR(KASP):overview of the technology and its application in crop improvement,Mol.Breed.,33(1):1-14
Spielmeyer W.,Singh R.P.,McFadden H.,Wellings C.R.,Huerta-Espino J.,Kong X.,Appels R.,and Lagudah E.S.,2008,Fine scale genetic and physical mapping using interstitial deletion mutants of Lr34/Yr18:a disease resistance locus effective against multiple p/thogens in wheat,Theor.Appl.Genet.,116(4):481-490
Terracciano I.,Maccaferri M.,Bassi F.,Mantovani P.,Sanguineti M.C.,Salvi S.,Simkova H.,Dolezel J.,Massi A.,Ammar K.,Kolmer J.,and Tuberosa R.,2013,Development of COS-SNP and HRM markers for high-throughput and reliable haplotype-based detection of Lr14a in durum wheat(Triticum durum Desf.),Theor.Appl.Genet.,126(4):1077-1101
Tyrka M.,and Chelkowski J.,2004,Enhancing the resistance of triticale by using genes from wheat and rye,J.Appl.Genet.,45(3):283-295
Wang S.C.,Wong D.,Forrest K.,Allen A.,Chao S.M.,Huang B.E.,Maccaferri M.,Salvi S.,Milner S.G.,Cattivelli L.,Mastrangelo A.M.,Whan A.,Stephen S.,Barker G.,Wieseke R.,Plieske J.,Lillemo M.,Mather D.,Appels R.,Dolferus R.,Brown-Guedira G.,Korol A.,Akhunova A.R.,Feuillet C.,Salse J.,Morgante M.,Pozniak C.,Luo M.C.,Dvorak J.,Morell M.,Dubcovsky J.,Ganal M.,Tuberosa R.,Lawley C.,Mikoulitch I.,Cavanagh C.,Edwards K.J.,Hayden M.,and Akhunov E.,2014,Characterization of polyploid wheat genomic diversity using a high-density 90 000 single nucleotide polymorphism array,Plant Biotechnol.J.,12(6):787-796
Wang S.H.,Gong K.Y.,Chu B.Y.,Sun Q.Y.,and Ma Z.H.,2018,Molecular detection of strip rust resistance gene(s)in 100wheat cultivars(lines)from Sichuan province in China,Zhiwu Bingli Xuebao(Acta Phytopathologica Sinica),48(2):195-206(王树和,龚凯悦,初炳瑶,孙秋玉,马占鸿,2018,四川省100个小麦品种(系)抗条锈病基因的分子检测,植物病理学报,48(2):195-206)
Wilhelm E.P.,Mackay I.J.,Saville R.J.,Korolev A.V.,Balfourier F.,Greenland A.J.,Boulton M.I.,and Powell W.,2013,Haplotype dictionary for the Rht-1 loci in wheat,Theor.Appl.Genet.,126(7):1733-1747
Xiao S.H.,Zhang X.Y.,Yan C.S.,and Lin H.,2002,Germplasm improvement for pre-harvest sprouting resistance in Chinese white-grained wheat:an overview of the current in strategy,Euphytica,126(1):35-38
Zhang D.Q.,Song X.M.,Feng J.,Ma W.J.,Wu B.J.,Zhang C.L.,Cui Z.X.,Feng Y.,and Sun D.J.,2016,Detection of dwarf genes Rht-B1b,Rht-D1b and Rht8 in Huang-huai valley winter wheat areas and their influences on agronomic characteristics,Mailei Zuowu Xuebao(Journal of Triticeae Crops),36(8):975-981(张德强,宋晓朋,冯洁,马文洁,武炳瑾,张传量,崔紫霞,冯毅,孙道杰,2016,黄淮麦区小麦品种矮秆基因Rht-B1b,Rht-D1b和Rht8的检测及其对农艺性状的影响,麦类作物学报,36(8):975-981)
Zhang H.J.,Su Z.Q.,Bai G.H.,Zhang X.,Ma H.X.,Li T.,Deng Y.,Mai C.Y.,Yu L.Q.,Liu H.W.,Yang L.,Li H.J.,and Zhou Y.,2018,Improvement of resistance of wheat cultivars to Fusaruum head blight in the Yellow-Huai rivers valley winer wheat zone with functional marker selection of Fhb1gene,Zuowu Xuebao(Acta Agronomica Sinica),44(4):505-511(张宏军,宿振起,柏贵华,张旭,马鸿翔,李腾,邓云,买春艳,于立强,刘宏伟,杨丽,李洪杰,周阳,2018,利用Fhb1基因功能标记选择提高黄淮冬麦区小麦品种对赤霉病的抗性,作物学报,44(4):505-511)
Zhang X.K.,Yang S.J.,Zhou Y.,He Z.H.,and Xia X.C.,2006,Distribution of the Rht-B1b,Rht-D1b and Rht8 reduced height genes in autumn-sown Chinese wheats detected by molecular markers,Euphytica,152(1):109-116
Zhang Y.B.,Sun L.F.,Xin W.L.,Song Q.J.,Zhang C.L.,Zhao H.B.,Xiao Z.M.,and Qi S.Y.,2003,Effect of HMW-GS 5+10on quality improvement in some leading wheat cultivars,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),36(3):242-247(张延滨,孙连发,辛文利,宋庆杰,张春利,赵海滨,肖志敏,祁适雨,2003,主栽小麦品种中5+10亚基对品质改良的影响,中国农业科学,36(3):242-247)
Zhou Y.,He Z.H.,Zhang G.S.,Xia L.Q.,Chen X.M.,Gao Y.C.,Jing Z.B.,and Yu G.J.,2004,Utilization of 1BL/1RS translocation in wheat breeding in China,Zuowu Xuebao(Acta Agronomica Sinica),30(6):531-535(周阳,何中虎,张改生,夏兰琴,陈新民,高永超,井赵斌,于广军,2004,1BL/IRS易位系在中国小麦育种中的应用,作物学报,30(6):531-535)
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