UPLC-PDA法测定蒲公英提取物中咖啡酸的含量

详细信息    查看全文 | 推荐本文 |

英文篇名：Determination of Caffeic Acid Content in Dandelion Extract by UPLC-PDA 作者：魏秀丽 ; 徐恩民 ; 刘霄飞 ; 李有志 ; 张秀英 ; 王海军 英文作者：WEI Xiu-li;XU En-min;LIU Xiao-fei;LI You-zhi;ZHANG Xiu-ying;WANG Hai-jun;Institute of Veterinary Drug Quality Inspection of Shandong Province;Shandong Provincial Key Laboratory of Quality Safty Monitoring and Risk Assessment for Animal Products;China Institute of Veterinary Medicine Inspection;Beijing Centre Biology Co.,Ltd;Beijing Veterinary Drugs Engineering Research Center; 关键词：蒲公英提取物 ; 超高效液相色谱-二极管阵列检测法 ; 咖啡酸 ; 含量测定 英文关键词：dandelion extract;;UPLC-PDA;;caffeic acid;;content determination 中文刊名：ZSYY 英文刊名：Chinese Journal of Veterinary Drug 机构：山东省兽药质量检验所;山东省畜产品质量安全监测与风险评估重点实验室;中国兽医药品监察所;北京生泰尔科技股份有限公司;北京市中兽药工程技术研究中心; 出版日期：2019-03-20 出版单位：中国兽药杂志 年：2019 期：v.53 基金：2015年国家科技支撑计划“兽药安全与质量评价技术研究与应用”编号“BAD11B03-01” 语种：中文; 页：ZSYY201903005 页数：5 CN：03 ISSN：11-2820/S 分类号：29-33

摘要

建立超高效液相色谱-二极管阵列检测法(UPLC-PDA法)测定蒲公英提取物中咖啡酸的含量。采用反相高效液相色谱法,用带有二极管阵列检测器的高效液相色谱仪(UPLC-PAD)对咖啡酸进行色谱分离和快速筛查。以ACQUITY UPLCTMHSS T3色谱柱(2.1 mm×100 mm,1.8μm)为分离柱,柱温:35℃;流动相体系:A项为乙腈,B项为0.4%磷酸水溶液,进行梯度洗脱;流速:0.35 m L/min;进样量:5μL;检测波长为323 nm。通过光谱图、保留时间及峰面积参数对咖啡酸进行定性定量检测。咖啡酸在1～100μg/m L的范围内线性良好(R2=0.999); 0.25μg/m L咖啡酸对照品溶液与空白溶液的信噪比>3,为检出限,1μg/m L咖啡酸对照品溶液与空白溶液的信噪比>10,为定量限,完全满足检测需求。该方法色谱分离较好,分析速度较快,前处理简单,适用于蒲公英提取物中咖啡酸的定性定量检测。
        This study was aimed to establish a high performance liquid chromatography-diode array( UPLC-PDA) test method for the determination of caffeic acid in dandelion extract. Caffeic acid can be separated and detected by reversed-phase high performance liquid chromatography( HPLC-PAD) with diode array detector.The test condition is like that: separate column is ACQUITY UPLCTMHSS T3 column( 2.1 mm×100 mm-1. 8 μ m),the column temperature is 35 ℃,mobile phase consists of acetonitrile and 0. 4% phosphoric acid solution for gradient elution,the flow rate is 0. 35 m L/min,the injection volume is 5 μ L,and the detection wavelength is323 nm. Caffeic acid is detected qualitatively and quantitatively by the retention time and peak response in the chromatogram.The linearity of the method for caffeic acid detection complies to the requirement at the range of 1 ～100 μg/m L( R2= 0.999).The limit of detection( LOD) is 0.25 μg/m L( S/N>3),and the limit of quantitation( LOQ) is 1 μg/m L( S/N > 10),which fully demonstrates the compliance of the test method. this method is suitable for qualitative and quantitative determination of caffeic acid in dandelion extract with fine separation,quick analysis and simple pretreatment procedure of the sample.

引文

[1]中国兽药典委员会.中华人民共和国兽药典二部2015年版[S].China Veterinary Pharmacopoeia Commission. The second Veterinary Pharmacopoeia of the people's Republic of China,2015[S].
    [2]国家药典委员会.中华人民共和国药典一部2015年版[S].State Pharmacopoeia Commission. The first part of the Pharmacopoeia of the people's Republic of China,2015[S].
    [3]褚娜.蒲公英提取物质量分析方法研究[D].黑龙江中医药大学,2015.Chu Na. Study on the quality analysis method of dandelion extract[D]. Heilongjiang University of traditional Chinese Medicine,2015.
    [4]贝京,于光福.高效液相色谱法测定蒲公英提取物中咖啡酸含量[J].中国野生植物资源,2010,29(03):54-56.Bei J,Yu G F. Determination of caffeic acid in dandelion extract by high performance liquid chromatography[J]. Wild Plant Resources of China,2010:29(03):54-56.
    [5]潘见,梁娟,谢慧明.蒲公英提取物的质量标准研究[J].时珍国医国药,2008(09):2064-2065.Pan S,Liang J,Xie H. Study on the quality standard of dandelion extract[J]. Shi Zhenguo Chinese Medicine,2008(09):2064-2065.
    [6]宋萍萍,王凌,蒋学华. HPLC测定注射用蒲公英冻干粉针剂中的绿原酸和咖啡酸[J].华西药学杂志,2010,25(03):346-348.Song P P,Wang L,Jiang X H. Determination of Lv Yuan acid and caffeic acid in dandelion lyophilized powder for injection by hplc[J]. Huaxi Journal of Pharmacy,2010-25(03):346-348.
    [7]彭德乾.蒲公英根部化学成分研究[D].齐齐哈尔大学,2012.Peng D Q. Studies on the chemical constituents of dandelion root[D]. Qiqihar University,2012.
    [8]林文艳.蒲公英化学成分研究及板蓝根HPLC指纹图谱研究[D].浙江大学,2005.Lin W Y. Studies on the Chemical constituents and hplc fingerprint of Radix Isatidis[D]. Zhejiang University,2005.