不同粒径天麻粉中有效成分的体外溶出度比较
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摘要
目的:研究不同粒径天麻粉末中有效成分(天麻素,对羟基苯甲醇,巴利森苷A,巴利森苷B,巴利森苷C)的体外溶出行为差异,为天麻超微粉的应用提供实验依据。方法:采用桨法对天麻粉末在不同溶出介质(水、人工胃液、人工肠液)中溶出度进行测定,利用HPLC测定不同粒径天麻粉中上述5种有效成分的溶出度,流动相乙腈-0.1%磷酸水溶液梯度洗脱,柱温40℃,检测波长220 nm。结果:在水中、人工肠液介质中,天麻细粉、极细粉中5种有效成分的溶出速率较天麻3种超微粉低;在人工胃液中,天麻超微粉中天麻素和对羟基苯甲醇的溶出速率较其他粉体高,但天麻超微粉中巴利森苷A的溶出速率较其他粉体低。结论:适度的微粉化能促进天麻粉末中有效成分的溶出,并不是越细越好,有效成分的溶出行为还受溶出介质的影响,可为天麻粉末临床应用时选择最佳粒径提供实验依据。
Objective: To compare in vitro dissolution behaviors of active ingredients( gastrodin,parishin A,p-hydroxybenzyl alcohol,parishin B and parishin C) in Gastrodiae Rhizoma powder with different particle size. Method: In vitro dissolution of Gastrodiae Rhizoma powder in different dissolution media( water,artificial gastric juice and artificial intestinal juice) were detected by stirring paddle method. Dissolution of these five components in Gastrodiae Rhizoma powder with different particle size was determined by HPLC,mobile phase was acetonitrile -0. 1% phosphoric acid solution for gradient elution, column temperature was set at 40 ℃ and detection wavelength was 220 nm. Result: In water and artificial intestinal juice, the dissolution rates of five active components in three kinds of Gastrodiae Rhizoma ultrafine powders were higher than that of the fine powder and the finest powder; in artificial gastric juice,the dissolution rates of gastrodin and p-hydroxybenzyl alcohol in Gastrodiae Rhizoma ultrafine powders were higher than that of the other powders, and the dissolution rate of parishin A in Gastrodiae Rhizoma ultrafine powders was lower than that of the other powders. Conclusion: An appropriate degree of superfine grinding can promote the dissolution of active ingredients in Gastrodiae Rhizoma,but not as fine as possible. The dissolution medium has an obvious influence on the dissolution behaviors of active components,which provides a reference for screening optical particle size of Gastrodiae Rhizoma powder in clinical application.
Objective: To compare in vitro dissolution behaviors of active ingredients( gastrodin,parishin A,p-hydroxybenzyl alcohol,parishin B and parishin C) in Gastrodiae Rhizoma powder with different particle size. Method: In vitro dissolution of Gastrodiae Rhizoma powder in different dissolution media( water,artificial gastric juice and artificial intestinal juice) were detected by stirring paddle method. Dissolution of these five components in Gastrodiae Rhizoma powder with different particle size was determined by HPLC,mobile phase was acetonitrile -0. 1% phosphoric acid solution for gradient elution, column temperature was set at 40 ℃ and detection wavelength was 220 nm. Result: In water and artificial intestinal juice, the dissolution rates of five active components in three kinds of Gastrodiae Rhizoma ultrafine powders were higher than that of the fine powder and the finest powder; in artificial gastric juice,the dissolution rates of gastrodin and p-hydroxybenzyl alcohol in Gastrodiae Rhizoma ultrafine powders were higher than that of the other powders, and the dissolution rate of parishin A in Gastrodiae Rhizoma ultrafine powders was lower than that of the other powders. Conclusion: An appropriate degree of superfine grinding can promote the dissolution of active ingredients in Gastrodiae Rhizoma,but not as fine as possible. The dissolution medium has an obvious influence on the dissolution behaviors of active components,which provides a reference for screening optical particle size of Gastrodiae Rhizoma powder in clinical application.
引文
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[14]梁兆昌,诸洪标,肖琳,等.杜仲超微粉体理化特性及体外溶出性能研究[J].中草药,2015,46(11):1609-1611.
[15]ZHAO X Y,DU F L,ZHU Q J,et al.Effect of superfine pulverzation on properties of Astragalus membranaceus powder[J].Powder Technol,2010,203(3):620-625.
[16]田紫平,肖慧,冯舒涵,等.天麻有效成分巴利森苷的降解规律分析[J].中国实验方剂学杂志,2017,23(23):18-21.
[2]黎光富,李刚凤,史荣荣.天麻多糖化学成分与药理作用研究综述[J].现代农业科技,2016(7):289-292.
[3]杨超,吕紫媛,伍端云.天麻的化学成分与药理机制研究进展[J].中国现代医生,2012,50(17):27-31.
[4]陶云海.天麻药理研究新进展[J].中国中药杂志,2008,23(1):108-110.
[5]罗刚,陈立庭,周晶.超微粉碎技术在中药研究中的应用[J].现代药物与临床,2011,26(2):108-112.
[6]张红刚,汪妮,李顺祥,等.超微粉碎技术对中药有效成分提取效果影响研究[J].广州化工,2013,41(16):63-64.
[7]陆付耳,阮金兰,余达经,等.论试微米中药[J].世界科学技术—中药现代化,2001,3(1):12-15.
[8]李凤生.超细粉体技术[M].北京:国防工业出版社,2000:373.
[9]赵唯君,刘荫贞,刘冲,等.天麻粉末粒度分布和有效成分溶出曲线研究[J].亚太传统医药,2017,13(9):19-22.
[10]杨芝芳,胡明勋,邓薇,等.天麻超微粉粒度对天麻素溶出率及抗惊厥作用的影响[J].中成药,2016,38(12):2700-2702.
[11]邱新建,贺凤成,张则平,等.五味子超微粉的含量及溶出度测定[J].中国实验方剂学杂志,2013,19(2):58-61.
[12]史国富,张璐.黄苓超微粉与普通粉溶出度比较[J].山西中医学院学报,2011,12(2):34-35.
[13]刘珈羽,郭换,肖佳雯,等.不同粒径白及粉的粉体学性质及体外溶出度比较[J].中国实验方剂学杂志,2018,24(3):25-29.
[14]梁兆昌,诸洪标,肖琳,等.杜仲超微粉体理化特性及体外溶出性能研究[J].中草药,2015,46(11):1609-1611.
[15]ZHAO X Y,DU F L,ZHU Q J,et al.Effect of superfine pulverzation on properties of Astragalus membranaceus powder[J].Powder Technol,2010,203(3):620-625.
[16]田紫平,肖慧,冯舒涵,等.天麻有效成分巴利森苷的降解规律分析[J].中国实验方剂学杂志,2017,23(23):18-21.