用范氏法测定多类植物中酸性洗涤木质素的影响因素研究
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摘要
探讨了用范氏法测定植物中酸性洗涤木质素(ADL)的影响因素,对不同消煮时间、不同硫酸浓度、不同浸泡时间条件下的测定结果进行了比较分析。结果表明:样品消煮的适宜时间为30 min,硫酸适宜浓度为72%～75%,浸泡适宜时间为3～4 h;人工检测结果的变异系数(CV)在2.23%～6.66%之间,仪器法的CV在0.15%～1.10%之间;人工检测与仪器法所测结果无显著性差异;范氏法与王玉万法所测结果无显著性差异,这两种方法适用于植物ADL的测定。
The author explored the factors affecting the determination of plant acid detergent lignin(ADL) by using Van Soest method, and compared and analyzed the determination results under different conditions. The results showed that: the suitable time for sample boiling was 30 min; the suitable concentration of sulfuric acid for sample decomposition was 72%～75%; the appropriate time for sample soaking was 3～4 h; the coefficient of variation(CV) of manual determination results was 2.23%～6.66%, while the CV of instrument determination results was 0.15%～1.10%; there was no significant difference in determination results between manual method and instrument method; there was also no significant difference in determination results between Van Soest method and Wang Yuwan's method, and these two methods were all suitable for the determination of plant ADL.
The author explored the factors affecting the determination of plant acid detergent lignin(ADL) by using Van Soest method, and compared and analyzed the determination results under different conditions. The results showed that: the suitable time for sample boiling was 30 min; the suitable concentration of sulfuric acid for sample decomposition was 72%～75%; the appropriate time for sample soaking was 3～4 h; the coefficient of variation(CV) of manual determination results was 2.23%～6.66%, while the CV of instrument determination results was 0.15%～1.10%; there was no significant difference in determination results between manual method and instrument method; there was also no significant difference in determination results between Van Soest method and Wang Yuwan's method, and these two methods were all suitable for the determination of plant ADL.
引文
[1] 李伟,熊谨,陈晓阳.木质素代谢的生理意义及其遗传控制研究进展[J].西北植物学报,2003,23(4):675-681.
[2] 孟永斌,徐蕾,张子东.木质素的研究进展[J].植物学研究,2016,5(1):17-25.
[3] 苏同福,高玉珍,刘霞,等.木质素的测定方法研究进展[J].河南农业大学学报,2007,41(3):356-362.
[4] LY/T 2151—2013,木材中纤维素和酸不溶木质素含量测定:近红外光谱法[S].
[5] GBT 20805—2006,饲料中酸性洗涤木质素(ADL)的测定[S].
[6] GB/T 1394—1990,食物中不溶性膳食纤维的测定方法[S].
[7] AOAC 973.18,动物饲料中的酸性洗涤纤维和木质素[S].
[8] 王玉万,徐文玉.木质纤维素固体基质发酵物中半纤维素、纤维素和木素的定量分析程序[J].微生物学报,1987(2):82-87.
[9] 郭小义,戴云辉,郭紫明,等.应用纤维素测定仪测定烟草中的纤维素[J].烟草科技,2009,48(1):58-62.
[10] 刘文静,潘葳,任丽花. FIBERTEC 2010半自动纤维分析仪测定饲料中纤维素、半纤维素、木质素的方法研究[J].福建农业学报,2013,28(7):722-726.
[11] GB/T 2677.8—1994,造纸原料酸不溶木素含量的测定[S].
[12] 陶用珍,管映亭.木质素的化学结构及其应用[J].纤维素科学与技术,2003,11(1):42-53.
[2] 孟永斌,徐蕾,张子东.木质素的研究进展[J].植物学研究,2016,5(1):17-25.
[3] 苏同福,高玉珍,刘霞,等.木质素的测定方法研究进展[J].河南农业大学学报,2007,41(3):356-362.
[4] LY/T 2151—2013,木材中纤维素和酸不溶木质素含量测定:近红外光谱法[S].
[5] GBT 20805—2006,饲料中酸性洗涤木质素(ADL)的测定[S].
[6] GB/T 1394—1990,食物中不溶性膳食纤维的测定方法[S].
[7] AOAC 973.18,动物饲料中的酸性洗涤纤维和木质素[S].
[8] 王玉万,徐文玉.木质纤维素固体基质发酵物中半纤维素、纤维素和木素的定量分析程序[J].微生物学报,1987(2):82-87.
[9] 郭小义,戴云辉,郭紫明,等.应用纤维素测定仪测定烟草中的纤维素[J].烟草科技,2009,48(1):58-62.
[10] 刘文静,潘葳,任丽花. FIBERTEC 2010半自动纤维分析仪测定饲料中纤维素、半纤维素、木质素的方法研究[J].福建农业学报,2013,28(7):722-726.
[11] GB/T 2677.8—1994,造纸原料酸不溶木素含量的测定[S].
[12] 陶用珍,管映亭.木质素的化学结构及其应用[J].纤维素科学与技术,2003,11(1):42-53.