HPLC法同时测定款冬花中7种成分
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摘要
目的建立HPLC法同时测定款冬Tussilago farfara L.花中绿原酸、芦丁、异槲皮苷、异绿原酸B、异绿原酸A、异绿原酸C、款冬酮的含有量。方法款冬花70%甲醇提取物的分析采用依利特Hypersil ODS2色谱柱(4.6 mm×250 mm,5μm);流动相甲醇-乙腈-0.1%磷酸溶液,梯度洗脱;检测波长220 nm;柱温25℃;体积流量1 mL/min。结果 7种成分在各自范围内线性关系良好(R~2≥0.999 4),平均加样回收率99.0%～102.2%,RSD 1.8%～2.8%。结论该方法准确稳定,重复性好,可用于款冬花的质量控制。
AIM To establish an HPLC method for the simultaneous content determination of chlorogenic acid, rutin, isoquercitrin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C and tussilagone in the flowers of Tussilago farfara L.. METHODS The analysis of 70% methanol extract of T. farfara was performed on a 25 ℃ thermostatic Elite Hypersil ODS2 column(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of methanol-acetonitrile-0.1% phosphoric acid flowing at 1 mL/min in a gradient elution manner,and the detection wavelength was set at 220 nm.RESULTS Seven constituents showed good linear relationships within their own ranges(R~2≥0.999 4), whose average recoveries were 99.0%-102.2% with the RSDs of 1.8%-2.8%.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of T. farfara.
AIM To establish an HPLC method for the simultaneous content determination of chlorogenic acid, rutin, isoquercitrin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C and tussilagone in the flowers of Tussilago farfara L.. METHODS The analysis of 70% methanol extract of T. farfara was performed on a 25 ℃ thermostatic Elite Hypersil ODS2 column(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of methanol-acetonitrile-0.1% phosphoric acid flowing at 1 mL/min in a gradient elution manner,and the detection wavelength was set at 220 nm.RESULTS Seven constituents showed good linear relationships within their own ranges(R~2≥0.999 4), whose average recoveries were 99.0%-102.2% with the RSDs of 1.8%-2.8%.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of T. farfara.
引文
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[12] 国家药典委员会.中华人民共和国药典:2015年版一部[S].北京:中国医药科技出版社,2015:332.
[13] 国家药典委员会.中华人民共和国药典:2015年版四部[S].北京:中国医药科技出版社,2015:31.
[2] 吴笛,张勉,张朝凤,等.款冬花中黄酮和酚酸类成分的研究[J].中国中药杂志,2010,35(9):1142-1144.
[3] Cushnie T P T,Lamb A J.Antimicrobial activity of flavonoids[J].Int J Antimicrob Agents,2005,26(5):343-356.
[4] Rigano D,Formisano C,Basile A,et al.Antibacterial activity of flavonoids and phenylpropanoids from Marrubium globosum ssp.libanoticum[J].Phytother Res,2007,21(4):395-397.
[5] Kimura M,Yamada H.Interaction in the antibacterial activity of flavonoids from Sophora japonica L.to Propionibacterium[J].Yakugaku Zasshi,1984,104(4):340-346.
[6] Scholz E,Heinrich M,Hunkler D.Caffeoylquinic acids and some biological activities of Pluchea symphytifolia[J].Planta Med,1994,60(4):360-364.
[7] Peluso G,De Feo V,De Simone F,et al.Studies on the inhibitory effects of caffeoylquinic acids on monocyte migration and superoxide ion production[J].J Nat Prod,1995,58(5):639-646.
[8] Góngora L,Giner R M,Máňez S,et al.Effects of caffeoyl conjugates of isoprenyl-hydroquinone glucoside and quinic acid on leukocyte function[J].Life Sci,2002,71(25):2995-3004.
[9] Wu Q Z,Zhao D X,Xiang J,et al.Antitussive,expectorant,and anti-inflammatory activities of four caffeoylquinic acids isolated from Tussilago farfara[J].Pharm Biol,2016,54(7):1117-1124.
[10] 李玮,杨秀伟.HPLC法同时测定款冬花中9个主要成分的含量[J].药物分析杂志,2012,32(9):1517-1524,1533.
[11] 何兵,刘艳,杨世艳,等.一测多评同时测定款冬花中10个成分的含量[J].药物分析杂志,2013,33(9):1518-1524.
[12] 国家药典委员会.中华人民共和国药典:2015年版一部[S].北京:中国医药科技出版社,2015:332.
[13] 国家药典委员会.中华人民共和国药典:2015年版四部[S].北京:中国医药科技出版社,2015:31.