三种人参属药用植物种子生根过程胚乳中人参皂苷的变化
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摘要
采用高效液相色谱-质谱联用技术对3种人参属药用植物种子生根过程胚乳中人参皂苷的变化进行了鉴定。结果表明,在人参胚乳中共鉴定出8种人参皂苷,西洋参中鉴定出8种人参皂苷,三七中鉴定出5种人参皂苷。在三者种子生根的过程中,胚乳中的皂苷含量总体呈上升趋势。当胚根伸长至5 mm时,人参、西洋参中皂苷含量上升不再明显,有些皂苷含量开始减少,但三七未出现上述现象。
The changes of ginsenosides in the endosperm of three medicinal plants of Panax species were identified by high performance liquid chromatography mass spectrometry.The results showed that eight ginsenosides were identified in endosperm of P.ginseng, eight ginsenosides were identified in P.quinquefolius, and five ginsenosides were identified in P.notoginseng. In the process of rooting of the three,the saponin content showed an overall upward trend. When the radicles were extended to 5 mm,the ginsenoside content of P.ginseng and P.quinquefolius rose no longer, and some saponin contents began to decrease. However, the above phenomenon did not occur on P.notoginseng.
The changes of ginsenosides in the endosperm of three medicinal plants of Panax species were identified by high performance liquid chromatography mass spectrometry.The results showed that eight ginsenosides were identified in endosperm of P.ginseng, eight ginsenosides were identified in P.quinquefolius, and five ginsenosides were identified in P.notoginseng. In the process of rooting of the three,the saponin content showed an overall upward trend. When the radicles were extended to 5 mm,the ginsenoside content of P.ginseng and P.quinquefolius rose no longer, and some saponin contents began to decrease. However, the above phenomenon did not occur on P.notoginseng.
引文
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[9]Kushiro T,Shibuya M,Ebizuka Y.-Amyrin synthase[J].European Journal of Biochemistry,1998,256(1):238-244.
[2]拱健婷,程新宇,孙萌,等.3种皂苷单体对三七种子萌发及幼苗生长的影响[J].江西农业大学学报,2015,(6):988-993.
[3]张崇禧,鲍建才,李向高,等.HPLC法测定人参、西洋参和三七不同部位中人参皂苷的含量[J].药物分析杂志,2005,(10):1190-1194.
[4]许云章,任烨,王静霞,等.不同生长年限、不同采收月份加拿大原产地西洋参根中9种人参皂苷和2种拟人参皂苷含量动态变化研究[J].中药材,2014,37(10):1743-1748.
[5]郑友兰,李向高.吉林人参与西洋参生药学和组织化学的比较研究[J].吉林农业大学学报,1986,(4):36-42.
[6]张元杰,周兰,来国防,等.整体柱HPLC法测定三七总皂苷中的5种皂苷成分[J].中国药师,2017,(10):1879-1881.
[7]Liang Y,Zhao S.Progress in understanding of ginsenoside biosynthesis[J].Plant Biology,2008,10(4):415-421.
[8]Chen X,Xue Y,Liu J H,et aL.Purification and characterization of glucosyltransferase from Panax Ginseng hairy root cultures[J].Pharmaceutical Biotechnology,2009,(1):14.
[9]Kushiro T,Shibuya M,Ebizuka Y.-Amyrin synthase[J].European Journal of Biochemistry,1998,256(1):238-244.