溴氰虫酰胺胁迫对小地老虎保护酶和解毒酶活性的诱导效应
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摘要
为明确小地老虎对溴氰虫酰胺的代谢解毒机制,采用浸叶法测定了溴氰虫酰胺对小地老虎3龄幼虫室内毒力,在此基础上,通过酶动力学法检测了溴氰虫酰胺亚致死剂量对小地老虎保护酶和解毒酶活力的影响,并比较了不同剂量处理后1、6、12、24、48和72 h时小地老虎体内保护酶和解毒酶活力的动态变化。结果表明,溴氰虫酰胺亚致死剂量初期可诱导过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活力升高;对羧酸酯酶(CarE)活力表现为早期诱导、后期抑制的作用;对谷胱甘肽S-转移酶(GSTs)活力表现为明显的诱导作用;对多功能氧化酶(MFOs)表现为一定程度的抑制作用。研究结果为深入了解小地老虎对溴氰虫酰胺的防御机理提供了一定的理论基础。
In order to clarify the mechanisms of metabolism and detoxification of Agrotis ipsilon after exposure to sublethal dose of cyantraniliprole, the leaf dipping method was used to determine the toxicity of cyantraniliprole against the 3 rd-instar larvae of A.ipsilon in the laboratory. The effects of sublethal dose of cyantraniliprole on the activity of protective enzymes and detoxification enzymes in A.ipsilon were detected by enzyme kinetic method, and the dynamic changes of the enzyme activities were compared at 1, 6, 12, 24, 48 and 72 h, respectively. The results showed that the activities of peroxidase(POD), catalase(CAT), and superoxide dismutase(SOD) were increased after exposed to sublethal doses of cyantraniliprole at the early stage of treatments, and the activity of carboxyl esterase(CarE) was induced at the early stage and inhibited at the later stage; the activity of glutathione S-transferase(GSTs) was significantly induced, and the activity of mixed function oxidase was, to some extent, inhibited after exposed to sublethal concentrations of cyantraniliprole. The results provide the theoretical basis for further understanding the defense mechanisms of A.ipsilon against cyantraniliprole.
In order to clarify the mechanisms of metabolism and detoxification of Agrotis ipsilon after exposure to sublethal dose of cyantraniliprole, the leaf dipping method was used to determine the toxicity of cyantraniliprole against the 3 rd-instar larvae of A.ipsilon in the laboratory. The effects of sublethal dose of cyantraniliprole on the activity of protective enzymes and detoxification enzymes in A.ipsilon were detected by enzyme kinetic method, and the dynamic changes of the enzyme activities were compared at 1, 6, 12, 24, 48 and 72 h, respectively. The results showed that the activities of peroxidase(POD), catalase(CAT), and superoxide dismutase(SOD) were increased after exposed to sublethal doses of cyantraniliprole at the early stage of treatments, and the activity of carboxyl esterase(CarE) was induced at the early stage and inhibited at the later stage; the activity of glutathione S-transferase(GSTs) was significantly induced, and the activity of mixed function oxidase was, to some extent, inhibited after exposed to sublethal concentrations of cyantraniliprole. The results provide the theoretical basis for further understanding the defense mechanisms of A.ipsilon against cyantraniliprole.
引文
[1] COSSENTINE J E. The experimental evolution on application microspondia against Agrotis ipsilon (Rottemberg) [J]. Entomophaga, 1987, 31(4): 323-330.
[2] 向玉勇,杨茂发.小地老虎在我国的发生危害及防治技术研究[J].安徽农业科学,2008,36(33):14636-14639.
[3] 向玉勇,杨茂发,李子忠.小地老虎室内连续饲养12代的化蛹率、羽化率和性比观察[J].黄山学院学报,2010,12(5):67-69.
[4] 李国清,王荫长.小地老虎的抗药性及几种增效剂增效作用的研究[J].农药,1995(10):19-22.
[5] 徐春梅.溴氰虫酰胺种子处理防治玉米田小地老虎[D].泰安:山东农业大学,2017.
[6] 杨建全,张玉珍.几种杀虫剂对小地老虎的毒力测定[J].生物安全学报,2000(1):53-56.
[7] 于伟丽,杜军辉,胡延萍,等.六种杀虫剂对小地老虎的毒力及对土壤生物安全性评价[J].植物保护学报,2012,39(3):277-282.
[8] 尹飞,陈焕瑜,冯夏,等.乙基多杀菌素抗性小菜蛾代谢解毒酶酶活性研究[J].应用昆虫学报,2016,53(2):314-319.
[9] 贾变桃,焦鹏,杨素梅.虱螨脲亚致死浓度对小菜蛾保护酶系和解毒酶系活力的影响[J].植物保护学报,2016,43(2):293-299.
[10] 刘喃喃,朱芳,徐强,等.昆虫抗药性机理:行为和生理改变及解毒代谢增强[J].昆虫学报,2006,49(4):671-679.
[11] RAKOTONDRAVELO M L, ANDERSON T D, CHARLTON R E, et al. Sublethal effects of three pesticides on activities of selected target and detoxification enzymes in the aquatic midge, Chironomus tentans (Diptera: Chironomidae)[J]. Archives of Environmental Contamination and Toxicology, 2006, 51(3): 360-366.
[12] VOJOUDI S, SABER M, HEJAZI M J, et al. Toxicity of chlorpyrifos, spinosad and abamectin on cotton bollworm, Helicoverpa armigera and their sublethal effects on fecundity and longevity [J].Bulletin of Insectology, 2011, 64(2): 189-193.
[13] RINGS R W, ARNOLD F J, KEASTER A J, et al. A worldwide, annotated bibliography of the black cutworm, Agrotis ipsilon (Hufnagel)[M]. Ohio: Ohio Agricultural Research and Development Center, 1974: 1-106.
[14] 陈国生.六索线虫防治小地老虎的小区试验[J].中国生物防治学报,1989,5(3):125-126.
[15] 李永禧.小地老虎生活习性及防治[J].昆虫知识,1964(1):3-7.
[16] 武海斌,范昆,张坤鹏,等.13种杀虫剂对小地老虎卵和幼虫的生物活性[J].环境昆虫学报,2013,35(3):409-414.
[17] 韩召军.小地老虎对几类杀虫剂的毒力反应及其抗药性变化[J].植物保护学报,1986(2):125-130.
[18] SALAMA H S. Strengthen control result of Bt. against Agrotis ipsilon (Rottemberg) [J]. Journal of Applied Entomology, 1989, 108(4): 372-380.
[19] 杨桂秋,黄琦,陈霖,等.新型杀虫剂溴氰虫酰胺研究概述[J].世界农药,2012,34(6):19-21.
[20] 杜军辉,于伟丽,王猛,等.三种双酰胺类杀虫剂对小地老虎和蚯蚓的选择毒性[J].植物保护学报,2013,40(3):266-272.
[21] 王猛,王凯,刘峰,等.溴氰虫酰胺和氯虫苯甲酰胺对三种鳞翅目害虫的毒力作用比较[J].植物保护学报,2014,41(3):360-366.
[22] SIMON L M, FATRAI Z, JONAS D E, et al. Study of peroxide metabolism enzymes during the development of Phaseolus vulgaris [J]. Biochemie und Physiologie der Pflanzen, 1974, 166(5/6): 387-392.
[23] 冯宏祖,刘映红,何林,等.阿维菌素和温度胁迫对朱砂叶螨自由基及保护酶活性的影响[J].植物保护学报,2008,35(6):530-536.
[24] 张金锋,薛庆中.稻飞虱为害胁迫对水稻植株内主要保护酶活性的影响[J].中国农业科学,2004,37(10):1487-1491.
[25] HAMA H, HOSODA A.High aliesterase activity and low acetylcholinestease sensitivity involved in organophosphorus and carbamate resistance of the brown planthopper, Nilaparvata lugens St?l (Homoptera: Delphacidae) [J]. Applied Entomology and Zoology, 1983, 18(4): 475-485.
[26] OPPENOORTH F J, PAS L J T V D, HOUX N W H. Glutathiones-transferase and hydrolytic activity in a tetrachlorvinphos-resistant strain of housefly and their influence on resistance [J]. Pesticide Biochemistry and Physiology, 1979, 11(1): 176-188.
[27] 田雪莲,尹显慧,龙友华,等.低剂量乙基多杀菌素对小菜蛾解毒酶的影响[J].农药学学报,2016,18(5):589-595.
[28] YU S J, NGUYEN S N. Detection and biochemical characterization of insecticide resistance in the diamondback moth [J]. Pesticide Biochemistry and Physiology, 1992, 44(1): 74-81.
[29] BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding [J]. Analytical Biochemistry, 1976, 72(1/2): 248-254.
[30] 郑雪松,赖添财,时立波,等.双酰胺类杀虫剂应用现状[J].农药,2012,51(8):554-557.
[31] 胡译文,孙建昌,耿阳阳,等.溴氰虫酰胺研究进展及应用现状[J].南方农业,2016,10(31):24-27.
[32] 杨桂秋,黄琦,陈霖,等.新型杀虫剂溴氰虫酰胺研究概述[J].世界农药,2012,34(6):19-21.
[33] 陈羿渠,向兴,贡常委,等.氯虫苯甲酰胺亚致死剂量对甜菜夜蛾主要解毒酶活性与生长繁殖的影响[J].中国农业科学,2017,50(8):1440-1451.
[34] XU C M, ZHANG Z Q, CUI K D, et al. Effects of sublethal concentrations of cyantraniliprole on the development, fecundity and nutritional physiology of the black cutworm Agrotis ipsilon (Lepidoptera: Noctuidae) [J/OL]. PLoS ONE, 2016, 11(6): e0156555.
[35] 张林林,李艳红,仵均祥.不同寄主植物对小地老虎生长发育和保护酶活性的影响[J].应用昆虫学报,2013,50(4):1049-1054.
[36] 韩永强,文礼章,侯茂林.水稻施用硅肥对稻纵卷叶螟幼虫保护酶和解毒酶活性的影响[J].应用昆虫学报,2016,53(3):482-490.
[37] 刘建业,钱蕾,蒋兴川,等.CO2浓度升高对西花蓟马和花蓟马成虫体内解毒酶和保护酶活性的影响[J].昆虫学报,2014,57(7):754-761.
[38] 刘玉坤,王渭霞,傅强,等.寄主植物对3种稻飞虱解毒酶和保护酶活性的影响[J].中国水稻科学,2011,25(6):659-666.
[39] 邢剑飞,刘艳,颜冬云.昆虫对拟除虫菊酯农药的抗性研究进展[J].环境科学与技术,2010,33(10):68-74.
[40] 罗雁婕,高希武,吴文伟,等.药剂对小菜蛾抗性及敏感品系乙酰胆碱酯酶抑制作用比较[J].农药学学报,2008,10(2):211-216.
[41] 何运转,李梅,冯国蕾,等.拟除虫菊酯对家蝇Na-K-ATPase抑制作用的研究[J].昆虫学报,1999,42(1):19-24.
[42] 魏波,郝赤,李会仙.杀虫剂亚致死剂量对棉铃虫靶标酶的影响[J].现代农药,2006,5(3):35-38.
[43] 贾变桃,洪珊珊,张雨超,等.阿维菌素对半闭弯尾姬蜂保护酶系和解毒酶系的影响[J].环境昆虫学报,2016,38(5):990-995.
[44] 廖月枝,严善春,李小平,等.甲氧虫酰肼对舞毒蛾幼虫保护酶活性的影响[J].林业科学,2011,47(9):93-99.
[45] 张文成,王开运,牛芳,等.虫螨腈胁迫对甜菜夜蛾保护酶系和解毒酶系的诱导效应[J].植物保护学报,2009,36(5):455-460.
[46] 杨琼,王淑会,张文慧,等.常用杀虫剂对异色瓢虫的毒力及其保护酶的影响[J].植物保护学报,2015,42(2):258-263.
[47] 齐浩亮,崔丽,王芹芹,等.溴虫氟苯双酰胺对小菜蛾的毒力及相关酶活性的影响[J].植物保护,2017,43(1):112-116.
[48] 余慧灵,向兴,袁贵鑫,等.溴氰虫酰胺亚致死剂量对甜菜夜蛾生长发育及体内解毒酶活性的影响[J].昆虫学报,2015,58(6):634-641.
[49] RUMPF S, HETZEL F, FRAMPTON C. Lacewings (Neuroptera: Hemerobiidae and Chrysopidae) and integrated pest management: enzyme activity as biomarker of sublethal insecticide exposure [J].Journal of Economic Entomology,1997,90(1): 102-108.
[2] 向玉勇,杨茂发.小地老虎在我国的发生危害及防治技术研究[J].安徽农业科学,2008,36(33):14636-14639.
[3] 向玉勇,杨茂发,李子忠.小地老虎室内连续饲养12代的化蛹率、羽化率和性比观察[J].黄山学院学报,2010,12(5):67-69.
[4] 李国清,王荫长.小地老虎的抗药性及几种增效剂增效作用的研究[J].农药,1995(10):19-22.
[5] 徐春梅.溴氰虫酰胺种子处理防治玉米田小地老虎[D].泰安:山东农业大学,2017.
[6] 杨建全,张玉珍.几种杀虫剂对小地老虎的毒力测定[J].生物安全学报,2000(1):53-56.
[7] 于伟丽,杜军辉,胡延萍,等.六种杀虫剂对小地老虎的毒力及对土壤生物安全性评价[J].植物保护学报,2012,39(3):277-282.
[8] 尹飞,陈焕瑜,冯夏,等.乙基多杀菌素抗性小菜蛾代谢解毒酶酶活性研究[J].应用昆虫学报,2016,53(2):314-319.
[9] 贾变桃,焦鹏,杨素梅.虱螨脲亚致死浓度对小菜蛾保护酶系和解毒酶系活力的影响[J].植物保护学报,2016,43(2):293-299.
[10] 刘喃喃,朱芳,徐强,等.昆虫抗药性机理:行为和生理改变及解毒代谢增强[J].昆虫学报,2006,49(4):671-679.
[11] RAKOTONDRAVELO M L, ANDERSON T D, CHARLTON R E, et al. Sublethal effects of three pesticides on activities of selected target and detoxification enzymes in the aquatic midge, Chironomus tentans (Diptera: Chironomidae)[J]. Archives of Environmental Contamination and Toxicology, 2006, 51(3): 360-366.
[12] VOJOUDI S, SABER M, HEJAZI M J, et al. Toxicity of chlorpyrifos, spinosad and abamectin on cotton bollworm, Helicoverpa armigera and their sublethal effects on fecundity and longevity [J].Bulletin of Insectology, 2011, 64(2): 189-193.
[13] RINGS R W, ARNOLD F J, KEASTER A J, et al. A worldwide, annotated bibliography of the black cutworm, Agrotis ipsilon (Hufnagel)[M]. Ohio: Ohio Agricultural Research and Development Center, 1974: 1-106.
[14] 陈国生.六索线虫防治小地老虎的小区试验[J].中国生物防治学报,1989,5(3):125-126.
[15] 李永禧.小地老虎生活习性及防治[J].昆虫知识,1964(1):3-7.
[16] 武海斌,范昆,张坤鹏,等.13种杀虫剂对小地老虎卵和幼虫的生物活性[J].环境昆虫学报,2013,35(3):409-414.
[17] 韩召军.小地老虎对几类杀虫剂的毒力反应及其抗药性变化[J].植物保护学报,1986(2):125-130.
[18] SALAMA H S. Strengthen control result of Bt. against Agrotis ipsilon (Rottemberg) [J]. Journal of Applied Entomology, 1989, 108(4): 372-380.
[19] 杨桂秋,黄琦,陈霖,等.新型杀虫剂溴氰虫酰胺研究概述[J].世界农药,2012,34(6):19-21.
[20] 杜军辉,于伟丽,王猛,等.三种双酰胺类杀虫剂对小地老虎和蚯蚓的选择毒性[J].植物保护学报,2013,40(3):266-272.
[21] 王猛,王凯,刘峰,等.溴氰虫酰胺和氯虫苯甲酰胺对三种鳞翅目害虫的毒力作用比较[J].植物保护学报,2014,41(3):360-366.
[22] SIMON L M, FATRAI Z, JONAS D E, et al. Study of peroxide metabolism enzymes during the development of Phaseolus vulgaris [J]. Biochemie und Physiologie der Pflanzen, 1974, 166(5/6): 387-392.
[23] 冯宏祖,刘映红,何林,等.阿维菌素和温度胁迫对朱砂叶螨自由基及保护酶活性的影响[J].植物保护学报,2008,35(6):530-536.
[24] 张金锋,薛庆中.稻飞虱为害胁迫对水稻植株内主要保护酶活性的影响[J].中国农业科学,2004,37(10):1487-1491.
[25] HAMA H, HOSODA A.High aliesterase activity and low acetylcholinestease sensitivity involved in organophosphorus and carbamate resistance of the brown planthopper, Nilaparvata lugens St?l (Homoptera: Delphacidae) [J]. Applied Entomology and Zoology, 1983, 18(4): 475-485.
[26] OPPENOORTH F J, PAS L J T V D, HOUX N W H. Glutathiones-transferase and hydrolytic activity in a tetrachlorvinphos-resistant strain of housefly and their influence on resistance [J]. Pesticide Biochemistry and Physiology, 1979, 11(1): 176-188.
[27] 田雪莲,尹显慧,龙友华,等.低剂量乙基多杀菌素对小菜蛾解毒酶的影响[J].农药学学报,2016,18(5):589-595.
[28] YU S J, NGUYEN S N. Detection and biochemical characterization of insecticide resistance in the diamondback moth [J]. Pesticide Biochemistry and Physiology, 1992, 44(1): 74-81.
[29] BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding [J]. Analytical Biochemistry, 1976, 72(1/2): 248-254.
[30] 郑雪松,赖添财,时立波,等.双酰胺类杀虫剂应用现状[J].农药,2012,51(8):554-557.
[31] 胡译文,孙建昌,耿阳阳,等.溴氰虫酰胺研究进展及应用现状[J].南方农业,2016,10(31):24-27.
[32] 杨桂秋,黄琦,陈霖,等.新型杀虫剂溴氰虫酰胺研究概述[J].世界农药,2012,34(6):19-21.
[33] 陈羿渠,向兴,贡常委,等.氯虫苯甲酰胺亚致死剂量对甜菜夜蛾主要解毒酶活性与生长繁殖的影响[J].中国农业科学,2017,50(8):1440-1451.
[34] XU C M, ZHANG Z Q, CUI K D, et al. Effects of sublethal concentrations of cyantraniliprole on the development, fecundity and nutritional physiology of the black cutworm Agrotis ipsilon (Lepidoptera: Noctuidae) [J/OL]. PLoS ONE, 2016, 11(6): e0156555.
[35] 张林林,李艳红,仵均祥.不同寄主植物对小地老虎生长发育和保护酶活性的影响[J].应用昆虫学报,2013,50(4):1049-1054.
[36] 韩永强,文礼章,侯茂林.水稻施用硅肥对稻纵卷叶螟幼虫保护酶和解毒酶活性的影响[J].应用昆虫学报,2016,53(3):482-490.
[37] 刘建业,钱蕾,蒋兴川,等.CO2浓度升高对西花蓟马和花蓟马成虫体内解毒酶和保护酶活性的影响[J].昆虫学报,2014,57(7):754-761.
[38] 刘玉坤,王渭霞,傅强,等.寄主植物对3种稻飞虱解毒酶和保护酶活性的影响[J].中国水稻科学,2011,25(6):659-666.
[39] 邢剑飞,刘艳,颜冬云.昆虫对拟除虫菊酯农药的抗性研究进展[J].环境科学与技术,2010,33(10):68-74.
[40] 罗雁婕,高希武,吴文伟,等.药剂对小菜蛾抗性及敏感品系乙酰胆碱酯酶抑制作用比较[J].农药学学报,2008,10(2):211-216.
[41] 何运转,李梅,冯国蕾,等.拟除虫菊酯对家蝇Na-K-ATPase抑制作用的研究[J].昆虫学报,1999,42(1):19-24.
[42] 魏波,郝赤,李会仙.杀虫剂亚致死剂量对棉铃虫靶标酶的影响[J].现代农药,2006,5(3):35-38.
[43] 贾变桃,洪珊珊,张雨超,等.阿维菌素对半闭弯尾姬蜂保护酶系和解毒酶系的影响[J].环境昆虫学报,2016,38(5):990-995.
[44] 廖月枝,严善春,李小平,等.甲氧虫酰肼对舞毒蛾幼虫保护酶活性的影响[J].林业科学,2011,47(9):93-99.
[45] 张文成,王开运,牛芳,等.虫螨腈胁迫对甜菜夜蛾保护酶系和解毒酶系的诱导效应[J].植物保护学报,2009,36(5):455-460.
[46] 杨琼,王淑会,张文慧,等.常用杀虫剂对异色瓢虫的毒力及其保护酶的影响[J].植物保护学报,2015,42(2):258-263.
[47] 齐浩亮,崔丽,王芹芹,等.溴虫氟苯双酰胺对小菜蛾的毒力及相关酶活性的影响[J].植物保护,2017,43(1):112-116.
[48] 余慧灵,向兴,袁贵鑫,等.溴氰虫酰胺亚致死剂量对甜菜夜蛾生长发育及体内解毒酶活性的影响[J].昆虫学报,2015,58(6):634-641.
[49] RUMPF S, HETZEL F, FRAMPTON C. Lacewings (Neuroptera: Hemerobiidae and Chrysopidae) and integrated pest management: enzyme activity as biomarker of sublethal insecticide exposure [J].Journal of Economic Entomology,1997,90(1): 102-108.