实时荧光重组酶介导核酸扩增在疟原虫快速检测中的应用研究
|
摘要
<正>疟疾是严重危害人类健康的全球性虫媒传染病之一,遍及全球90多个国家和地区,主要分布在热带和亚热带,世界上超过一半的人口生活在疟疾流行区[1]。据统计,疟疾每年感染数千万人,仅在非洲,每年有50万5岁以下儿童死于疟疾,已成为全球最重要的公共卫生问题之一[2]。疟疾的早期诊断和治疗是其防治的关键[3]。
引文
[1]李欢,孙菲,文岚,等.多重PCR快速检测恶性疟和间日疟的研究[J].中国人兽共患病学报,2014,30(3):288-291.
[2]王真瑜,江莉,蔡黎,等.环介导等温扩增技术检测间日疟原虫方法的建立及应用分析[J].热带医学杂志,2012,12(2):157-161.
[3]汪圣强,周华云,李宗,等.SYBR GreenⅠ染料法定量PCR用于人体疟原虫定量检测及虫种鉴别的研究[J].中国血吸虫病防治杂志,2012,23(6):677-681.
[4]Payne D.Use and limitations of light microscopy for diagnosing malaria at the primary health care level[J].Bull World Health Organ,1988,66(5):621-626.
[5]蒙锋,王善青,徐华章.双重免疫色谱技术检测恶性疟和间日疟的初步观察[J].中国寄生虫病防治杂志,2000,13(2):90-91.
[6]王龙英,张述义,王克泰,等.免疫色谱技术(ICT)用于疟疾诊断的探讨[J].实用寄生虫病杂志,1999,7(4):189.
[7]Sethabutr O,Brown AE,Panyim S,et al.Detection of Plasmodium falciparum by polymerase chain reaction in a field study[J].J Infect Dis,1992,166(1):145-148.
[8]Link L,Bart A,Verhaar N,et al.Molecular detection of Plasmodium knowlesi in a Dutch traveler by real-time PCR[J].J Clin Microbiol,2012,50(7):2523-2524.
[9]Taylor BJ,Martin KA,Arango E,et al.Real-time PCR detection of Plasmodium directly from whole blood and filter paper samples[J].Malar J,2011,10:244.
[10]Kamau E,Tolbert LS,Kortepeter L,et al.Development of a highly sensitive genus-specific quantitative reverse transcriptase realtime PCR assay for detection and quantitation of Plasmodium by amplifying RNA and DNA of the 18S rRNA genes[J].J Clin Microbiol,2011,49(8):2946-2953.
[11]江莉,王真瑜,马晓疆,等.多重PCR种特异性检测恶性疟原虫和间日疟原虫方法的建立[J].国际医学寄生虫病杂志,2009,36(2):78-82.
[12]Genc A,Eroglu F,Koltas IS.Detection of Plasmodium vivax by nested PCR and real-time PCR[J].Korean J Parasitol,2010,48(2):99-103.
[13]Poon LL,Wong BW,Ma EH,et al.Sensitive and inexpensive molecular test for falciparum malaria:detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification[J].Clin Chem,2006,52(2):303-306.
[14]杨秋林,许丽芳,沈元琼,等.环介导等温扩增技术检测恶性疟原虫的研究[J].中国人兽共患病学报,2008,24(11):1045-1046.
[15]Notomi T,Okayama H,Masubuchi H,et al.Loop-mediated isothermal amplification of DNA[J].Nucleic Acids Res,2000,28(12):E63.
[16]吕蓓,程海荣,严庆丰,等.用重组酶介导扩增技术快速扩增核酸[J].中国科学.生命科学,2010,40(10):983-988.
[17]吕蓓,程海荣,严庆丰,等.体外核酸快速扩增技术的发展和不断创新[J].中国生物工程杂志,2011,31(3):91-96.
[2]王真瑜,江莉,蔡黎,等.环介导等温扩增技术检测间日疟原虫方法的建立及应用分析[J].热带医学杂志,2012,12(2):157-161.
[3]汪圣强,周华云,李宗,等.SYBR GreenⅠ染料法定量PCR用于人体疟原虫定量检测及虫种鉴别的研究[J].中国血吸虫病防治杂志,2012,23(6):677-681.
[4]Payne D.Use and limitations of light microscopy for diagnosing malaria at the primary health care level[J].Bull World Health Organ,1988,66(5):621-626.
[5]蒙锋,王善青,徐华章.双重免疫色谱技术检测恶性疟和间日疟的初步观察[J].中国寄生虫病防治杂志,2000,13(2):90-91.
[6]王龙英,张述义,王克泰,等.免疫色谱技术(ICT)用于疟疾诊断的探讨[J].实用寄生虫病杂志,1999,7(4):189.
[7]Sethabutr O,Brown AE,Panyim S,et al.Detection of Plasmodium falciparum by polymerase chain reaction in a field study[J].J Infect Dis,1992,166(1):145-148.
[8]Link L,Bart A,Verhaar N,et al.Molecular detection of Plasmodium knowlesi in a Dutch traveler by real-time PCR[J].J Clin Microbiol,2012,50(7):2523-2524.
[9]Taylor BJ,Martin KA,Arango E,et al.Real-time PCR detection of Plasmodium directly from whole blood and filter paper samples[J].Malar J,2011,10:244.
[10]Kamau E,Tolbert LS,Kortepeter L,et al.Development of a highly sensitive genus-specific quantitative reverse transcriptase realtime PCR assay for detection and quantitation of Plasmodium by amplifying RNA and DNA of the 18S rRNA genes[J].J Clin Microbiol,2011,49(8):2946-2953.
[11]江莉,王真瑜,马晓疆,等.多重PCR种特异性检测恶性疟原虫和间日疟原虫方法的建立[J].国际医学寄生虫病杂志,2009,36(2):78-82.
[12]Genc A,Eroglu F,Koltas IS.Detection of Plasmodium vivax by nested PCR and real-time PCR[J].Korean J Parasitol,2010,48(2):99-103.
[13]Poon LL,Wong BW,Ma EH,et al.Sensitive and inexpensive molecular test for falciparum malaria:detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification[J].Clin Chem,2006,52(2):303-306.
[14]杨秋林,许丽芳,沈元琼,等.环介导等温扩增技术检测恶性疟原虫的研究[J].中国人兽共患病学报,2008,24(11):1045-1046.
[15]Notomi T,Okayama H,Masubuchi H,et al.Loop-mediated isothermal amplification of DNA[J].Nucleic Acids Res,2000,28(12):E63.
[16]吕蓓,程海荣,严庆丰,等.用重组酶介导扩增技术快速扩增核酸[J].中国科学.生命科学,2010,40(10):983-988.
[17]吕蓓,程海荣,严庆丰,等.体外核酸快速扩增技术的发展和不断创新[J].中国生物工程杂志,2011,31(3):91-96.