凹大叶蝉线粒体全基因组序列分析及系统发育关系
|
摘要
凹大叶蝉(Bothrogonia ferruginea)是我国一类重要的农业经济害虫,测序和分析凹大叶蝉线粒体基因组,并在线粒体水平上探讨凹大叶蝉在头喙亚目中的系统发育地位,对凹大叶蝉系统分类研究具有重要的意义。本研究采用PCR扩增与克隆测序技术,拼接获得凹大叶蝉线粒体基因组全序列,基于蛋白质编码基因的串联序列构建系统发育树。凹大叶蝉线粒体基因组全长15 262 bp (GenBank No.KU167550),包括13个蛋白编码基因(protein-coding genes, PCGs),22个tRNA基因,2个rRNA基因和一段A+T富集区,呈现出与头喙亚目昆虫一致的核苷酸组成和基因排布顺序。基因组碱基A+T含量为76.48%,AT偏度率为0.170 2。基因重叠区域共计14处,基因间隔区域共计10处。A+T富含区内存在ATTTA碱基序列并在其后存在一个典型的微卫星结构(TA)25。叶蝉科昆虫A+T富集区的串联重复区被发现存在ATCTA和CCCTCT两段特殊结构,而角蝉科和其他头喙亚目昆虫未发现此特殊结构。头喙亚目22个种基于线粒体基因组的系统发育关系发现为沫蝉总科+(蜡蝉总科+角蝉总科),凹大叶蝉与檬果褐叶蝉(Idioscopus nitidulus)等4种叶蝉归属于头喙亚目角蝉总科叶蝉科。凹大叶蝉线粒体基因组的基因排布与叶蝉科其他昆虫完全相同,基于线粒体基因组的头喙亚目系统发育关系与传统形态学研究结果一致:凹大叶蝉归属于头喙亚目角蝉总科叶蝉科。本研究为头喙亚目系统分类的深入研究提供了数据资料。
Bothrogonia ferruginea is an important agricultural and economic pest in China. Sequencing and analyzing the complete mitochondrial genome of B. ferruginea and exploring its phylogenetic status in the suborder Auchenorrhyncha at the mitochondrial level are of great significance to its systematic taxonomy. The complete mitochondrial genome sequence of B. ferruginea was obtained by PCR amplification and sequencing, and its general features and base composition were analyzed. The plyogenetic tree of mitochondrial protein-coding genes of 22 species of Auchenorrhyncha and 2 outgroups was constructed by using the Bayesian inference(BI) and maximum likelihood method(ML). The complete mitochondrial genome of B. ferruginea was 15 262 bp(GenBank No. KU167550), including 13 protein-coding genes(PCGs), 22 tRNA genes, 2 rRNA genes and one control region. The nucleotide composition and gene distribution sequence of B. ferruginea showed the same as insect of Auchenorrhyncha. The A + T content of mitochondrial genome is 76.48%, and the AT-skew is 0.170 2. There were 14 overlapping genes and 10 intergenic regions. All protein-coding genes use the ATN as initiation codon. Majority of PCGs had a complete termination codon(TAA or TAG), except that COⅡ gene terminated with the incomplete stop codon TA. All the predicted tRNAs showed the classic clover-leaf structure, except for the absence of DHU(dihydrouracil)arm of tRNASer(AGN). The A + T rich region contained some conserved sequence(ATTTA) and a typical microsatellite structure(TA)25. Two special structures, ATCTA and CCCTCT, were found in the tandem repeats in the control region of Cicadellidae, but not in the family Membracidae and other Auchenorrhyncha. The phylogenetic relationships of 22 species of Auchenorrhyncha based on mitochondrial genome were identified as Cercopoidea +(Fulgoroidea + Membracoidea). B. ferruginea, Nephotettix cincticeps, Idioscopus nitidulus,Empoasca vitis and Homalodisca vitripennis belonged to Auchenorrhyncha, Membracoidea and Cicadellidae.The gene arrangement pattern of the mitochondrial genome of B. ferruginea was identical to those of other Cicadellidae insects. The phylogenetic relationship of Auchenorrhyncha constructed based on the mitochondrial genome was consistent with those in traditional morphological taxonomy. B. ferruginea belonged to Auchenorrhyncha, Membracoidea and Cicadellidae. Our research provides data for the further study of auchenorrhyncha classification system.
Bothrogonia ferruginea is an important agricultural and economic pest in China. Sequencing and analyzing the complete mitochondrial genome of B. ferruginea and exploring its phylogenetic status in the suborder Auchenorrhyncha at the mitochondrial level are of great significance to its systematic taxonomy. The complete mitochondrial genome sequence of B. ferruginea was obtained by PCR amplification and sequencing, and its general features and base composition were analyzed. The plyogenetic tree of mitochondrial protein-coding genes of 22 species of Auchenorrhyncha and 2 outgroups was constructed by using the Bayesian inference(BI) and maximum likelihood method(ML). The complete mitochondrial genome of B. ferruginea was 15 262 bp(GenBank No. KU167550), including 13 protein-coding genes(PCGs), 22 tRNA genes, 2 rRNA genes and one control region. The nucleotide composition and gene distribution sequence of B. ferruginea showed the same as insect of Auchenorrhyncha. The A + T content of mitochondrial genome is 76.48%, and the AT-skew is 0.170 2. There were 14 overlapping genes and 10 intergenic regions. All protein-coding genes use the ATN as initiation codon. Majority of PCGs had a complete termination codon(TAA or TAG), except that COⅡ gene terminated with the incomplete stop codon TA. All the predicted tRNAs showed the classic clover-leaf structure, except for the absence of DHU(dihydrouracil)arm of tRNASer(AGN). The A + T rich region contained some conserved sequence(ATTTA) and a typical microsatellite structure(TA)25. Two special structures, ATCTA and CCCTCT, were found in the tandem repeats in the control region of Cicadellidae, but not in the family Membracidae and other Auchenorrhyncha. The phylogenetic relationships of 22 species of Auchenorrhyncha based on mitochondrial genome were identified as Cercopoidea +(Fulgoroidea + Membracoidea). B. ferruginea, Nephotettix cincticeps, Idioscopus nitidulus,Empoasca vitis and Homalodisca vitripennis belonged to Auchenorrhyncha, Membracoidea and Cicadellidae.The gene arrangement pattern of the mitochondrial genome of B. ferruginea was identical to those of other Cicadellidae insects. The phylogenetic relationship of Auchenorrhyncha constructed based on the mitochondrial genome was consistent with those in traditional morphological taxonomy. B. ferruginea belonged to Auchenorrhyncha, Membracoidea and Cicadellidae. Our research provides data for the further study of auchenorrhyncha classification system.
引文
陈志腾,杜予州.2016.昆虫线粒体基因组重排的研究进展[J].环境昆虫学报,38(4):843-851.(Chen Z T,Du Y Z.2016.Research progress on insect mitochondrial genome rearrangement[J].Journal of Environmental Entomology,38(4):843-851.)
葛钟麟.中国经济昆虫志(第十册,同翅目:叶蝉科)[M].科学出版社,北京,1966:60-62.(Ge Z L.1966.Chinese economic insects(Volume 10,Homopetera:Cicadellidae)[M].Science Publishing Press,Beijing,60-62.)
郭仲龙,袁明龙.2016.半翅目昆虫线粒体基因组学研究进展[J].中国科学:生命科学,46(2):151-166.(Guo J L,Yuan M L.2016.Advances in mitochondrial genomics of Hemiptera insects[J].Chinese Science:Life Sciences,46(2):151-166.)
胡家勇.2014.猕猴桃溃疡病菌株收集及其寄主和传播媒介的鉴定[D].硕士学位论文,安徽农业大学,导师:朱立武.pp.2-6.(Hu J Y.2014.Collection of Pseudomonas syringae pv.actinidiae strain and their hosts and vectors identification[D].Thesis for M.S.,Anhui Agriculture University,Supervisor:Zhu L W.pp.2-6.)
孟泽洪.2009.中国大叶蝉亚科分子系统学研究(半翅目,叶蝉科)[D].硕士学位论文,贵州大学,导师:杨茂发.pp.45-72.(Meng H Z.2009.Molecular systematics of the leafhopper subfamily cicadellinae(Insecta:Hemiotera:Cicadellidae)[D].Thesis for M.S.,Guizhou university,Supervisor:Yang M F.pp.45-72.)
宋南,江志毅.2012.蜡蝉总科(昆虫纲:半翅目)系统分类研究进展[J].四川动物,31(2):317-322.(Song N,Jiang ZY.2012.Studiea on systematics of fulgoroidea(Insecta:Hemiptera)[J].Sichuan Journal of Zoology,31(2):317-322.)
杨集昆,李法圣.1980.黑尾大叶蝉考订-凹大叶蝉属二十二新种记述(同翅目:大叶蝉科)[J].昆虫分类学报,2(3):191-210.(Yang J K,Li S F,1980.Notes on Bothrogonia(Homoptera:Cicadellidae)with descriptions of 22new species from China[J].Entomotaxonomia,2(3):191-210.)
俞鹏飞,吴国火,韩宝瑜.2017.白边大叶蝉线粒体基因组测序与分析[J].安徽农业大学学报,44(5):874-881.(Yu P F,Wu G H,Han B Y.2017b.Sequencing and analysis of the mitochondrial genome of Kolla paulula(Walker)(Hemiptera:Cicadellidae)[J].Journal of Anhui Agricultural University,44(5):874-811.)
张熏尹.2014.黑尾大叶蝉(Bothrogonia ferruginea)生活史及媒介葡萄皮尔斯病之传播效率探讨[D].硕士学位论文,国立中兴大学,导师:段淑人.pp.58.(Zhang Y X.2014.Life history of Bothrogonia ferruginea and its transmission efficiency of Xylella fastidiosa[D].Thesis for M.S.,National Chung Hsing University,Supervisor:Duan S R.pp.58.)
赵兴.2011.角蝉总科(昆虫纲:半翅目)部分种类线粒体基因组及分子系统发育的研究[D].博士学位论文,中国科学院大学,导师:梁爱萍.pp.26-66.(Zhao X,2011.Studies on the mitochondrial genome and molecular phylogeny of some species of the family Cicadoptera(Insecta:Hemiptera)[D].Thesis for Ph.D.,University of Chinese Academy of Sciences,Supervisor:Liang A P.pp.26-66.)
Bae J S,Kim I,Sohn H D,et al.2004.The mitochondrial genome of the firefly,Pyrocoelia rufa:Complete DNA sequence,genome organization,and phylogenetic analysis with other insects[J].Molecular Phylogenetics and Evolution,32:978-985.
Benson G.1999.Tandem repeats finder:A program to analyze DNA sequences[J].Nucleic Acids Research,27:573-580.
Burland T G.2000.DNASTAR's Lasergene sequence analysis software[J].Methods in Molecular Biology,132:71-91.
Cameron S L,Lambkin C L,Barker S C,et al.2007.A mitochondrial genome phylogeny of Diptera:Whole genome sequence data accurately resolve relationships over broad timescales with high precision[J].Systematic Entomology,32:40-59.
Carver M,Gross G E,Woodward T E.1991.The insects of Australia[M].Cornell University Press,New York.pp.429-509.
Cui Y,Xie Q,Hua J,et al.2013.Phylogenomics of Hemiptera(Insecta:Paraneoptera)based on mitochondrial genomes[J].Systematic Entomology,38(1):233-245.
Dai R H,Wang J J,Yang M F.2018.The complete mitochondrial genome of the leafhopper Idioscopus clypealis(Hemiptera:Cicadellidae:Idiocerinae)[J].Mitochondrial DNA Part B,3(1):32-33.
Dietrich C H,Rakitov R A,Holmes J L,et al.2001.Phylogeny of the major lineages of Membracoidea(Insecta:Hemiptera:Cicadomorpha)based on 28S r DNA sequences[J].Molecular Phylogenetics and Evolution,18(2):293-305.
Dmitriev D A.2010.Homologies of the head of Membracoidea based on nymphal morphology with notes on other groups of Auchenorrhyncha(Hemiptera)[J].European Journal of Entomology,107(4):597-613.
D'Urso V.2002.The wing-coupling apparatus of Hemiptera Auchenorrhyncha:Structure,function,and systematic value[J].Denisia,176(4):401-410.
Grimaldi D,Engel M S.2005.Evolution of the insects[M].Cambridge University Press,New York.pp.98-100.
Guindon S,Gascuel O.2003.A simple,fast,and accurate algorithm to estimate large phylogenies by maximum likelihood[J].Systematic Biology,52(5):696-704.
He S L,Zou Y,Zhang L F,et al.2015.The complete mitochondrial genome of the Beet Webworm,Spoladea recurvalis(Lepidoptera:Crambidae)and its phylogenetic implications[J].Plos One,10(6):e0129355.
Hua J,Li M,Dong P,et al.2008.Comparative and phylogenomic studies on the mitochondrial genomes of Pentatomomorpha(Insecta:Hemiptera:Heteroptera)[J].BMCGenomics,9(1):610.
Huelsenbeck J P,Ronquist F.2001.MRBAYES:Bayesian inference of phylogenetic trees[J].Bioinformatics,17(8):754-755.
Li Q,Wei S J,Shi M,et al.2015.The mitochondrial genome of Diadromus collaris(Hymenoptera:Ichneumonidae)[J].Mitochondrial DNA,26(2):303-304.
Liang A P,Gao J,Zhao X.2016.Characterization of the complete mitochondrial genome of the treehopper Darthula hardwickii(Hemiptera:Aetalionidae)[J].Mitochondrial DNA,27(5):3291-3292.
Liu C,Chang J,Ma C,et al.2013.Mitochondrial genomes of two Sinochlora species(Orthoptera):Novel genome rearrangements and recognition sequence of replication origin[J].BMC Genomics,14:114.
Lowe T M,Chan P P.2016.tRNAscan-SE on-line:Search and contextual analysis of transfer RNA genes[J].Nucleic Acids Research,44(W1):54-57.
Ojala D,Montoya J,Attardi G.1981.tRNA punctuation model of RNA processing in human mitochondria[J].Nature,290:470-474.
Okimoto R,Macfarlane J,Clary D,et al.1922.The mitochondrial genomes of two nematodes,Caenorhabditis elegans and Ascaris suum[J].Genetics,130(3):471-498.
Perna N T,Kocher T D.1995.Unequal base frequencies and the estimation of substitution rates[J].Molecular Biology and Evolution,12(2):359-361.
Ross H H.1964.General catalogue of the Homoptera.fascicleⅥ.Cicadelloidea.bibliography of the Cicadelloidea(Homoptera:Auchenorhyncha)[J].Bulletin of the Ecological Society of America,10(4):235-236.
Simon C,Beckenbach A T.2006.Incorporating molecular evolution into phylogenetic analysis,and a new compilation of conserved polymerase chain reaction primers for animal mitochondrial DNA[J].Annual Review of Ecology,Evolution,and Systematics,37(37):545-579.
Stewart J B,Beckenbach A T.2005.Insect mitochondrial genomics:The complete mitochondrial genome sequence of the meadow spittlebug Philaenus spumarius(Hemiptera:Auchenorrhyncha:Cercopoidae)[J].Genome,48(1):46-54.
Taanman J W.1999.The mitochondrial genome:Structure,transcription,translation and replication[J].Biochimica et Biophysica Acta,1410(2):103-123.
Tamura K,Stecher G,Peterson D,et al.2013.MEGA6:Molecular evolutionary genetics analysis version 6.0[J].Molecular Biology and Evolution,30:2725-2729.
Tuan S J,Hu F T,Chang H Y,et al.2016.Xylella fastidiosa transmission and life history of two Cicadellinae sharpshooters,Kolla paulula and Bothrogonia ferruginea(Hemiptera:Cicadellidae),in Taiwan[J].Journal of Economic Entomology,109(3):tow016.
Vila M,Bj?rklund M.2004.The utility of the neglected mitochondrial control region for evolutionary studies in Lepidoptera(Insecta)[J].Journal of Molecular Evolution,58(3):280-290.
Vogel D S,Axelrod R C.2002.Predicting the effects of gene deletion[J].ACM SIGKDD Explorations Newsletter,4(2):101-103.
Wang H L,Yang J,Boykin L M,et al.2013.The characteristics and expression profiles of the mitochondrial genome for the Mediterranean species of the Bemisia tabaci complex[J].BMC Genomics,14(25):1-15.
Wu Y,Dai R,Zhan H,et al.2016.Complete mitochondrial genome of Drabescoides nuchalis(Hemiptera:Cicadellidae)[J].Mitochondrial DNA,27(5):3625-3627.
Yu P,Wang M,Cui L,et al.2017.The complete mitochondrial genome of Tambocerus sp.(Hemiptera:Cicadellidae)[J].DNA Sequence,28(1):133-134.
Zhang D X,Szymura J M,Hewitt G M.1995.Evolution and structural conservation of the control region of insect mitochondrial DNA[J].Journal of Molecular Evolution,40(4):382-391.
Zhao X,Liang A P.2015.Complete DNA sequence of the mitochondrial genome of the treehopper Leptobelus gazella(Membracoidea:Hemiptera)[J].Mitochondrial DNA,27(5):3291-3292.
Zhou N,Wang M,Cui L,et al.2014.Complete mitochondrial genome of Empoasca vitis(Hemiptera:Cicadellidae)[J].Mitochondrial DNA,27(2):1052-1053.
葛钟麟.中国经济昆虫志(第十册,同翅目:叶蝉科)[M].科学出版社,北京,1966:60-62.(Ge Z L.1966.Chinese economic insects(Volume 10,Homopetera:Cicadellidae)[M].Science Publishing Press,Beijing,60-62.)
郭仲龙,袁明龙.2016.半翅目昆虫线粒体基因组学研究进展[J].中国科学:生命科学,46(2):151-166.(Guo J L,Yuan M L.2016.Advances in mitochondrial genomics of Hemiptera insects[J].Chinese Science:Life Sciences,46(2):151-166.)
胡家勇.2014.猕猴桃溃疡病菌株收集及其寄主和传播媒介的鉴定[D].硕士学位论文,安徽农业大学,导师:朱立武.pp.2-6.(Hu J Y.2014.Collection of Pseudomonas syringae pv.actinidiae strain and their hosts and vectors identification[D].Thesis for M.S.,Anhui Agriculture University,Supervisor:Zhu L W.pp.2-6.)
孟泽洪.2009.中国大叶蝉亚科分子系统学研究(半翅目,叶蝉科)[D].硕士学位论文,贵州大学,导师:杨茂发.pp.45-72.(Meng H Z.2009.Molecular systematics of the leafhopper subfamily cicadellinae(Insecta:Hemiotera:Cicadellidae)[D].Thesis for M.S.,Guizhou university,Supervisor:Yang M F.pp.45-72.)
宋南,江志毅.2012.蜡蝉总科(昆虫纲:半翅目)系统分类研究进展[J].四川动物,31(2):317-322.(Song N,Jiang ZY.2012.Studiea on systematics of fulgoroidea(Insecta:Hemiptera)[J].Sichuan Journal of Zoology,31(2):317-322.)
杨集昆,李法圣.1980.黑尾大叶蝉考订-凹大叶蝉属二十二新种记述(同翅目:大叶蝉科)[J].昆虫分类学报,2(3):191-210.(Yang J K,Li S F,1980.Notes on Bothrogonia(Homoptera:Cicadellidae)with descriptions of 22new species from China[J].Entomotaxonomia,2(3):191-210.)
俞鹏飞,吴国火,韩宝瑜.2017.白边大叶蝉线粒体基因组测序与分析[J].安徽农业大学学报,44(5):874-881.(Yu P F,Wu G H,Han B Y.2017b.Sequencing and analysis of the mitochondrial genome of Kolla paulula(Walker)(Hemiptera:Cicadellidae)[J].Journal of Anhui Agricultural University,44(5):874-811.)
张熏尹.2014.黑尾大叶蝉(Bothrogonia ferruginea)生活史及媒介葡萄皮尔斯病之传播效率探讨[D].硕士学位论文,国立中兴大学,导师:段淑人.pp.58.(Zhang Y X.2014.Life history of Bothrogonia ferruginea and its transmission efficiency of Xylella fastidiosa[D].Thesis for M.S.,National Chung Hsing University,Supervisor:Duan S R.pp.58.)
赵兴.2011.角蝉总科(昆虫纲:半翅目)部分种类线粒体基因组及分子系统发育的研究[D].博士学位论文,中国科学院大学,导师:梁爱萍.pp.26-66.(Zhao X,2011.Studies on the mitochondrial genome and molecular phylogeny of some species of the family Cicadoptera(Insecta:Hemiptera)[D].Thesis for Ph.D.,University of Chinese Academy of Sciences,Supervisor:Liang A P.pp.26-66.)
Bae J S,Kim I,Sohn H D,et al.2004.The mitochondrial genome of the firefly,Pyrocoelia rufa:Complete DNA sequence,genome organization,and phylogenetic analysis with other insects[J].Molecular Phylogenetics and Evolution,32:978-985.
Benson G.1999.Tandem repeats finder:A program to analyze DNA sequences[J].Nucleic Acids Research,27:573-580.
Burland T G.2000.DNASTAR's Lasergene sequence analysis software[J].Methods in Molecular Biology,132:71-91.
Cameron S L,Lambkin C L,Barker S C,et al.2007.A mitochondrial genome phylogeny of Diptera:Whole genome sequence data accurately resolve relationships over broad timescales with high precision[J].Systematic Entomology,32:40-59.
Carver M,Gross G E,Woodward T E.1991.The insects of Australia[M].Cornell University Press,New York.pp.429-509.
Cui Y,Xie Q,Hua J,et al.2013.Phylogenomics of Hemiptera(Insecta:Paraneoptera)based on mitochondrial genomes[J].Systematic Entomology,38(1):233-245.
Dai R H,Wang J J,Yang M F.2018.The complete mitochondrial genome of the leafhopper Idioscopus clypealis(Hemiptera:Cicadellidae:Idiocerinae)[J].Mitochondrial DNA Part B,3(1):32-33.
Dietrich C H,Rakitov R A,Holmes J L,et al.2001.Phylogeny of the major lineages of Membracoidea(Insecta:Hemiptera:Cicadomorpha)based on 28S r DNA sequences[J].Molecular Phylogenetics and Evolution,18(2):293-305.
Dmitriev D A.2010.Homologies of the head of Membracoidea based on nymphal morphology with notes on other groups of Auchenorrhyncha(Hemiptera)[J].European Journal of Entomology,107(4):597-613.
D'Urso V.2002.The wing-coupling apparatus of Hemiptera Auchenorrhyncha:Structure,function,and systematic value[J].Denisia,176(4):401-410.
Grimaldi D,Engel M S.2005.Evolution of the insects[M].Cambridge University Press,New York.pp.98-100.
Guindon S,Gascuel O.2003.A simple,fast,and accurate algorithm to estimate large phylogenies by maximum likelihood[J].Systematic Biology,52(5):696-704.
He S L,Zou Y,Zhang L F,et al.2015.The complete mitochondrial genome of the Beet Webworm,Spoladea recurvalis(Lepidoptera:Crambidae)and its phylogenetic implications[J].Plos One,10(6):e0129355.
Hua J,Li M,Dong P,et al.2008.Comparative and phylogenomic studies on the mitochondrial genomes of Pentatomomorpha(Insecta:Hemiptera:Heteroptera)[J].BMCGenomics,9(1):610.
Huelsenbeck J P,Ronquist F.2001.MRBAYES:Bayesian inference of phylogenetic trees[J].Bioinformatics,17(8):754-755.
Li Q,Wei S J,Shi M,et al.2015.The mitochondrial genome of Diadromus collaris(Hymenoptera:Ichneumonidae)[J].Mitochondrial DNA,26(2):303-304.
Liang A P,Gao J,Zhao X.2016.Characterization of the complete mitochondrial genome of the treehopper Darthula hardwickii(Hemiptera:Aetalionidae)[J].Mitochondrial DNA,27(5):3291-3292.
Liu C,Chang J,Ma C,et al.2013.Mitochondrial genomes of two Sinochlora species(Orthoptera):Novel genome rearrangements and recognition sequence of replication origin[J].BMC Genomics,14:114.
Lowe T M,Chan P P.2016.tRNAscan-SE on-line:Search and contextual analysis of transfer RNA genes[J].Nucleic Acids Research,44(W1):54-57.
Ojala D,Montoya J,Attardi G.1981.tRNA punctuation model of RNA processing in human mitochondria[J].Nature,290:470-474.
Okimoto R,Macfarlane J,Clary D,et al.1922.The mitochondrial genomes of two nematodes,Caenorhabditis elegans and Ascaris suum[J].Genetics,130(3):471-498.
Perna N T,Kocher T D.1995.Unequal base frequencies and the estimation of substitution rates[J].Molecular Biology and Evolution,12(2):359-361.
Ross H H.1964.General catalogue of the Homoptera.fascicleⅥ.Cicadelloidea.bibliography of the Cicadelloidea(Homoptera:Auchenorhyncha)[J].Bulletin of the Ecological Society of America,10(4):235-236.
Simon C,Beckenbach A T.2006.Incorporating molecular evolution into phylogenetic analysis,and a new compilation of conserved polymerase chain reaction primers for animal mitochondrial DNA[J].Annual Review of Ecology,Evolution,and Systematics,37(37):545-579.
Stewart J B,Beckenbach A T.2005.Insect mitochondrial genomics:The complete mitochondrial genome sequence of the meadow spittlebug Philaenus spumarius(Hemiptera:Auchenorrhyncha:Cercopoidae)[J].Genome,48(1):46-54.
Taanman J W.1999.The mitochondrial genome:Structure,transcription,translation and replication[J].Biochimica et Biophysica Acta,1410(2):103-123.
Tamura K,Stecher G,Peterson D,et al.2013.MEGA6:Molecular evolutionary genetics analysis version 6.0[J].Molecular Biology and Evolution,30:2725-2729.
Tuan S J,Hu F T,Chang H Y,et al.2016.Xylella fastidiosa transmission and life history of two Cicadellinae sharpshooters,Kolla paulula and Bothrogonia ferruginea(Hemiptera:Cicadellidae),in Taiwan[J].Journal of Economic Entomology,109(3):tow016.
Vila M,Bj?rklund M.2004.The utility of the neglected mitochondrial control region for evolutionary studies in Lepidoptera(Insecta)[J].Journal of Molecular Evolution,58(3):280-290.
Vogel D S,Axelrod R C.2002.Predicting the effects of gene deletion[J].ACM SIGKDD Explorations Newsletter,4(2):101-103.
Wang H L,Yang J,Boykin L M,et al.2013.The characteristics and expression profiles of the mitochondrial genome for the Mediterranean species of the Bemisia tabaci complex[J].BMC Genomics,14(25):1-15.
Wu Y,Dai R,Zhan H,et al.2016.Complete mitochondrial genome of Drabescoides nuchalis(Hemiptera:Cicadellidae)[J].Mitochondrial DNA,27(5):3625-3627.
Yu P,Wang M,Cui L,et al.2017.The complete mitochondrial genome of Tambocerus sp.(Hemiptera:Cicadellidae)[J].DNA Sequence,28(1):133-134.
Zhang D X,Szymura J M,Hewitt G M.1995.Evolution and structural conservation of the control region of insect mitochondrial DNA[J].Journal of Molecular Evolution,40(4):382-391.
Zhao X,Liang A P.2015.Complete DNA sequence of the mitochondrial genome of the treehopper Leptobelus gazella(Membracoidea:Hemiptera)[J].Mitochondrial DNA,27(5):3291-3292.
Zhou N,Wang M,Cui L,et al.2014.Complete mitochondrial genome of Empoasca vitis(Hemiptera:Cicadellidae)[J].Mitochondrial DNA,27(2):1052-1053.