瓜蒌皮药材中五种黄酮类成分的含量测定
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摘要
目的:建立瓜蒌皮药材中五种黄酮类成分的含量测定方法。方法:采用高效液相色谱法测定不同收集地瓜蒌皮药材中芦丁、异槲皮苷、槲皮苷、槲皮素、山奈酚的含量,并以各成分作为指标进行主成分分析和聚类分析。结果:不同收集地瓜蒌皮药材中黄酮类各成分含量差异较大,异槲皮苷累积方差贡献率为95. 514%,聚类分析结果表明安徽1的瓜蒌皮药材质量最好。结论:以异槲皮苷为公共因子变量,对瓜蒌皮药材进行含量测定可以更好地控制瓜蒌皮药材的质量。
Objective: To establish a method for the determination of contents of five flavonoids in Trichosanthis Pericarpium.Methods: The contents of rutin,isoquercitrin,quercitrin,quercetin,kaempferol from different collection areas were determined by HPLC and each component was taken as the indexes for principal component analysis and cluster analysis. Results: The contents of flavonoids in Trichosanthis Pericarpium were significantly different in different collection areas. The cumulative variance contribution rate of isoquercetin was 95. 514%. The results of cluster analysis showed that the quality of Trichosanthis Pericarpium in Anhui 1 was the best. Conclusion: With isoquercetin as the common factor variable,the quality of Trichosanthis Pericarpium could be better controlled by the content determination.
Objective: To establish a method for the determination of contents of five flavonoids in Trichosanthis Pericarpium.Methods: The contents of rutin,isoquercitrin,quercitrin,quercetin,kaempferol from different collection areas were determined by HPLC and each component was taken as the indexes for principal component analysis and cluster analysis. Results: The contents of flavonoids in Trichosanthis Pericarpium were significantly different in different collection areas. The cumulative variance contribution rate of isoquercetin was 95. 514%. The results of cluster analysis showed that the quality of Trichosanthis Pericarpium in Anhui 1 was the best. Conclusion: With isoquercetin as the common factor variable,the quality of Trichosanthis Pericarpium could be better controlled by the content determination.
引文
[1]国家药典委员会.中华人民共和国药典:一部[S].北京:中国医药科技出版社,2015:114.
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[11]潘平,韩谷鸣.穿心莲黄酮片治疗冠心病的临床研究[J].放射免疫学杂志,1999,12(1):30-32.
[12]张振贤,胡文文.醋柳黄酮片治疗冠心病心绞痛(瘀血证)101例[J].辽宁中医杂志,1997,24(6):18-19.
[13]任继刚,周琳,赵大林.复方芦丁片对冠心病患者抗血小板药物所致皮下出血的影响[J].中国医药指南,2009,7(19):83-84.
[14]舒毅,谭陶,张思宇,等.槲皮素的药理学研究进展[J].华西药学杂志,2008,23(6):689-691.
[2]吕建新.瓜蒌皮注射液治疗急性心肌梗死室性再灌注心律失常32例[J].浙江中医杂志,2011,46(6):467.
[3]史国玉,刘伟,耿岩玲,等.瓜蒌皮药材的HPLC指纹图谱[J].中国实验方剂学杂志,2014,20(1):73-76.
[4]杨丽,杨玲.瓜蒌皮对冠心病的药理作用及其机制研究[J].临床医药文献电子杂志,2016,3(37):7495-7496.
[5]朱玉霞,陈锡阳,陈秋.瓜蒌皮在痰浊内阻证冠心病治疗中的应用[J].上海医药,2013,34(17):25-28.
[6]王祝枝,王晓华,朱玉,等.不同产地瓜蒌皮药材中槲皮素及总黄酮含量测定[J].中国实验方剂学杂志,2014,20(19):86-89.
[7]于京平.栝楼果皮生药学研究[D].济南:山东中医药大学,2014.
[8]孔浩,郭庆梅,郭新苗,等.柱前衍生高效液相色谱法测定瓜蒌皮中游离氨基酸含量[J].山东中医药大学学报,2014,38(3):254-256.
[9]郝变,袁少雄,潘丽丽,等.瓜蒌皮多糖的单糖组成及含量测定方法研究[J].中华中医药杂志,2015,30(6):2153-2156.
[10]屠婕红,黄佳.瓜蒌皮中水溶性多糖的提取及含量测定[J].时珍国医国药,2009,20(2):281-282.
[11]潘平,韩谷鸣.穿心莲黄酮片治疗冠心病的临床研究[J].放射免疫学杂志,1999,12(1):30-32.
[12]张振贤,胡文文.醋柳黄酮片治疗冠心病心绞痛(瘀血证)101例[J].辽宁中医杂志,1997,24(6):18-19.
[13]任继刚,周琳,赵大林.复方芦丁片对冠心病患者抗血小板药物所致皮下出血的影响[J].中国医药指南,2009,7(19):83-84.
[14]舒毅,谭陶,张思宇,等.槲皮素的药理学研究进展[J].华西药学杂志,2008,23(6):689-691.