硒提高马铃薯幼苗抗寒性生理研究
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摘要
低温胁迫是影响我国南方冬闲田马铃薯种植的一大阻碍因素,发生在马铃薯植株生长发育前期。以马铃薯品种金湘为试材,研究不同硒浓度(1、3、5 mg·L~(-1))和适宜浓度的喷硒次数(1、2、3次)对马铃薯幼苗抗寒性的影响。结果表明:外源喷施1、3、5 mg·L~(-1)亚硒酸钠处理均可以提高低温胁迫下马铃薯叶片中叶绿素含量,降低叶片相对电导率以及丙二醛(MDA)、H_2O_2含量,且3 mg·L~(-1)的喷施效果最佳。低温胁迫下喷施3 mg·L~(-1)亚硒酸钠溶液1～3次的幼苗叶绿素含量高于未喷硒对照,MDA和H_2O_2含量均降低,且喷施2次时效果最好。综上,适宜的喷硒浓度(3 mg·L~(-1))和次数(2次)能在一定程度上降低低温胁迫所带来的伤害,从而提高马铃薯幼苗的抗寒性。
Low temperature stress is a major obstacle to potato growth and development especially during the early stage in winter fallowing fields in southern China.Taking potato variety‘Jinxiang’as test material,this paper studied the effects of spraying Na_2SeO_3 with different selenium concentrations(1,3,5 mg·L~(-1))and different times(1,2,3)on cold resistance of potato seedlings respectively.The results showed that exogenous spraying of 1,3,5 mg·L~(-1) selenium solution could increase the chlorophyll contents of potato leaves,reduce the relative conductivity,contents of MDA and hydrogen peroxide of leaves under low temperature stress.The effect of spraying 3 mg·L~(-1) was the best.The chlorophyll content of seedlings by spraying 3 mg·L~(-1) selenium solution for 1-3 times was higher than that of without selenium spraying under low temperature stress,and the contents of malondialdehyde and hydrogen peroxide were all decreased.The effect of spraying twice was the best.To sum up,the suitable selenium concentration(3 mg·L~(-1))and spraying times(twice)can reduce the damage caused by low temperature stress to certain extent,thus having improved the cold resistance of potato seedlings.
Low temperature stress is a major obstacle to potato growth and development especially during the early stage in winter fallowing fields in southern China.Taking potato variety‘Jinxiang’as test material,this paper studied the effects of spraying Na_2SeO_3 with different selenium concentrations(1,3,5 mg·L~(-1))and different times(1,2,3)on cold resistance of potato seedlings respectively.The results showed that exogenous spraying of 1,3,5 mg·L~(-1) selenium solution could increase the chlorophyll contents of potato leaves,reduce the relative conductivity,contents of MDA and hydrogen peroxide of leaves under low temperature stress.The effect of spraying 3 mg·L~(-1) was the best.The chlorophyll content of seedlings by spraying 3 mg·L~(-1) selenium solution for 1-3 times was higher than that of without selenium spraying under low temperature stress,and the contents of malondialdehyde and hydrogen peroxide were all decreased.The effect of spraying twice was the best.To sum up,the suitable selenium concentration(3 mg·L~(-1))and spraying times(twice)can reduce the damage caused by low temperature stress to certain extent,thus having improved the cold resistance of potato seedlings.
引文
陈爱葵,韩瑞宏,李东洋,凌连莲,罗惠霞,唐上剑.2010.植物叶片相对电导率测定方法比较研究.广东教育学院学报,30(5):88-91.
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贾玮,吴隽,屈婵娟,胡承孝,王富华,赵小虎.2015.硒增强植物抗逆能力及其机理研究进展.中国农学通报,31(14):171-176.
林艳,郭伟珍,徐振华,贾宗锴.2012.大叶女贞耐冻性及冬季叶片丙二醛和可溶性糖含量的变化.中国农学通报,28(25):68-72.
秦玉芝,刘明月,宋勇,胡新喜,何长征,王万兴,周华兰,熊兴耀.2017.马铃薯新品种金湘.中国蔬菜,(11):101-102.
孙丽.2016.外源硒对低温胁迫下草莓幼苗的缓解效应及对As A-GSH循环的影响[硕士论文].杭州:浙江大学.
吴之琳,尹雪斌,袁林喜,刘颖,李淼.2014.硒在植物抗逆境胁迫耐受中的作用.粮食科技与经济,39(3):26-31.
张小莉,王鹏程,宋纯鹏.2009.植物细胞过氧化氢的测定方法.植物学报,44(1):103-106.
张艳嫣.2013.外源硒对低温胁迫下铁皮石斛幼苗的缓解效应及其抗氧化生理特征的变化[硕士论文].杭州:浙江大学.
Hawrylak-Nowak B,Matraszek R,Szymańska M.2010.Selenium modifies the effect of short-term chilling stress on cucumber plants.Biological Trace Element Research,138:307-315.
Sabaty M,Avazeri C,Pignol D.2001.Characterization of the reduction of selenate and tellurite by nitrate reductases.Applied&Environmental Microbiology,67(11):5122-5126.
陈芳,胡小松.1998.马铃薯块茎“低温糖化”机理的研究及进展.中国马铃薯,(1):52-55.
贾玮,吴隽,屈婵娟,胡承孝,王富华,赵小虎.2015.硒增强植物抗逆能力及其机理研究进展.中国农学通报,31(14):171-176.
林艳,郭伟珍,徐振华,贾宗锴.2012.大叶女贞耐冻性及冬季叶片丙二醛和可溶性糖含量的变化.中国农学通报,28(25):68-72.
秦玉芝,刘明月,宋勇,胡新喜,何长征,王万兴,周华兰,熊兴耀.2017.马铃薯新品种金湘.中国蔬菜,(11):101-102.
孙丽.2016.外源硒对低温胁迫下草莓幼苗的缓解效应及对As A-GSH循环的影响[硕士论文].杭州:浙江大学.
吴之琳,尹雪斌,袁林喜,刘颖,李淼.2014.硒在植物抗逆境胁迫耐受中的作用.粮食科技与经济,39(3):26-31.
张小莉,王鹏程,宋纯鹏.2009.植物细胞过氧化氢的测定方法.植物学报,44(1):103-106.
张艳嫣.2013.外源硒对低温胁迫下铁皮石斛幼苗的缓解效应及其抗氧化生理特征的变化[硕士论文].杭州:浙江大学.
Hawrylak-Nowak B,Matraszek R,Szymańska M.2010.Selenium modifies the effect of short-term chilling stress on cucumber plants.Biological Trace Element Research,138:307-315.
Sabaty M,Avazeri C,Pignol D.2001.Characterization of the reduction of selenate and tellurite by nitrate reductases.Applied&Environmental Microbiology,67(11):5122-5126.