血清淀粉样蛋白A、C反应蛋白和降钙素原联合检测在新生儿感染诊断中的应用
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摘要
【目的】探讨血清淀粉样蛋白A(SAA)、C反应蛋白(CRP)及降钙素原(PCT)检测在新生儿感染性疾病早期诊断中的价值。【方法】分别采用ELISA和化学发光方法检测30例新生儿败血症和34例病毒感染患儿血清SAA、CRP、PCT水平,并与正常对照组新生儿进行比较。【结果】败血症组血清SAA、CRP、PCT水平与对照组比较差异均有统计学意义(P<0.01);病毒感染组中血清SAA水平与对照组比较差异有显著统计学意义(P<0.01),而CRP水平与对照组比较差异有统计学意义(P<0.05)。败血症组血清SAA、CRP、PCT阳性率分别为96.7%、73.3%、90.0%;病毒感染组血清SAA、CRP、PCT阳性率分别为100.0%、29.4%、8.8%,其中SAA与CRP和PCT比较差异均有显著统计学意义(P<0.01)。【结论】血清SAA、CRP、PCT水平的联合连续检测可以作为新生儿感染性疾病早期诊断和判断病情的客观指标,有助于鉴别细菌感染与病毒感染。
[Objective] To investigate the value of serum amyloid A(SAA),C-reactive protein(CRP) and procalcitonin(PCT) in early diagnosis of neonatal infective diseases. [Methods] The levels of SAA,CRP and PCT in 30 cases of neonates with neonatal septicemia and 34 cases of virus infection were detected by ELISA and chemiluminescence respectively,and compared with the normal control group. [Results] There were statistically significant differences in serum SAA,CRP and PCT levels between the septicemia group and the control group(P<0.01);there were statistically significant differences in serum SAA levels between the viral infection group and the control group(P<0.01),while CRP levels was statistically significant different as compared with those of the control(P<0.05).The serum SAA,CRP and PCT positive rates were 96.7%,73.3% and 90% respectively in the septicemia group,and 100%,29.4% and 8.8% respectively in the viral infection group.There were statistically significant differences between SAA,CRP and PCT(P<0.01). [Conclusion] The combined detection of serum SAA,CRP and PCT can be used as an objective index for early diagnosis and judgement of neonatal infective diseases and help to differentiate bacterial and viral infections.
[Objective] To investigate the value of serum amyloid A(SAA),C-reactive protein(CRP) and procalcitonin(PCT) in early diagnosis of neonatal infective diseases. [Methods] The levels of SAA,CRP and PCT in 30 cases of neonates with neonatal septicemia and 34 cases of virus infection were detected by ELISA and chemiluminescence respectively,and compared with the normal control group. [Results] There were statistically significant differences in serum SAA,CRP and PCT levels between the septicemia group and the control group(P<0.01);there were statistically significant differences in serum SAA levels between the viral infection group and the control group(P<0.01),while CRP levels was statistically significant different as compared with those of the control(P<0.05).The serum SAA,CRP and PCT positive rates were 96.7%,73.3% and 90% respectively in the septicemia group,and 100%,29.4% and 8.8% respectively in the viral infection group.There were statistically significant differences between SAA,CRP and PCT(P<0.01). [Conclusion] The combined detection of serum SAA,CRP and PCT can be used as an objective index for early diagnosis and judgement of neonatal infective diseases and help to differentiate bacterial and viral infections.
引文
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[10]张智洁,刘勇,孙继梅,等.头孢他啶影响大鼠降钙素原产生机制的初步研究[J].中华医院感染学杂志,2007,17(2):129-133.
[11]马超,赵春玉,王元祥,等.牛磺酸对婴幼儿体外循环术下血浆降钙素原的影响及意义[J].中国当代医药,2010,17(24):9-10,13.
[12]费凤英,衣萍,林见敏.血清淀粉样蛋白A与C反应蛋白联合检测的临床应用价值[J].检验医学,2014,29(10):1031-1033.
[13]姜剑巍,杨宇,应春妹.乳胶增强免疫散射比浊法检测血清淀粉样蛋白A的应用评价[J].检验医学,2015,30(1):49-52.
[14]陈长强,顾志冬,樊绮诗.血清淀粉样蛋白A在疾病应用中的研究进展[J].检验医学,2012,27(9):776-779.
[2]SATAR M,OZLü F.Neonatal sepsis:a continuing disease burden[J].Turk J Pediatr,2012,54(5):449-457.
[3]MEEM M,MODAK J K,MORTUZA R,et al.Biomarkers for diagnosis of neonatal infections:a systematic analysis of their potential as a point-of-care diagnostics[J].J Glob Health,2011,1(2):201-209.
[4]何梅.晚期先兆流产孕妇血清C-反应蛋白的相关分析[J].现代医药卫生,2004,20(18):185l-1852.
[5]冯建飞,王军,张利,等.新生儿感染中IL-8和C反应蛋白的检测价值[J].小儿急救医学,2004,11(4):224-225.
[6]ERLANDSN E J,RENDERS E.Reference interval for serum C-reactive protein in healthy blood donors using the Dade Behring N Latex CRP mono assay[J].Scand J Clin Lab Invest,2000,60(1):37-43.
[7]ROHDE L E P,HENNEKENS C H,RIDKER P M.Survey of C-reactive protein and cardiovascular risk factors in apparently healthy men[J].Am J Cardiol,1999,84(9):1018-1022.
[8]BECKER K L,SNIDER R,NYLON E S.Procalcitonin in sepsis and systemic inflammation:a harmful biomarker and a therapeutic target[J].Br J Pharmacol,2010,159(2):253-264.
[9]GHORBANI G.Procalcitonin role in differential diagnosis of infection stages and non infection inflammation[J].Pak J Biol Sci,2009,12(4):393-396.
[10]张智洁,刘勇,孙继梅,等.头孢他啶影响大鼠降钙素原产生机制的初步研究[J].中华医院感染学杂志,2007,17(2):129-133.
[11]马超,赵春玉,王元祥,等.牛磺酸对婴幼儿体外循环术下血浆降钙素原的影响及意义[J].中国当代医药,2010,17(24):9-10,13.
[12]费凤英,衣萍,林见敏.血清淀粉样蛋白A与C反应蛋白联合检测的临床应用价值[J].检验医学,2014,29(10):1031-1033.
[13]姜剑巍,杨宇,应春妹.乳胶增强免疫散射比浊法检测血清淀粉样蛋白A的应用评价[J].检验医学,2015,30(1):49-52.
[14]陈长强,顾志冬,樊绮诗.血清淀粉样蛋白A在疾病应用中的研究进展[J].检验医学,2012,27(9):776-779.