2017年北京市丰台区6例乙型Victoria系流感病毒HA1基因特征分析
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摘要
目的了解2017年北京市丰台区流感暴发疫情中乙型Victoria系(BV)流感毒株血凝素(HA1)基因特征和变异情况。方法对北京市丰台区2017年6起BV流感暴发疫情病例标本中分离得到的6株毒株的HA1基因进行提核酸、扩增和测序,通过DNAStar 7.1、Bioedit v7.0.9、Mega 6.0.6等生物信息学软件对序列进行分析。结果 6例样本中1号样本与2017年部分日本流行毒株亲缘关系较近(99.6%);另5例样本与2016年我国厦门、泰国和肯尼亚部分流行毒株的亲缘关系较紧密(99.1%～99.8%)。6例样本与WHO推荐的疫苗株B/Brisbane/60/2008的同源性为98.6%。6例样本共有7个位点发生了变异,其中有5例样本发生了I132V、N144D和E200D的变异,分别涉及到抗原决定簇A、B、D3个区。结论北京市丰台区2017年BV系流感株的HA1基因发生了个别位点的变异,但与WHO推荐的疫苗株B/Brisbane/60/2008的匹配度相对较高,继续使用疫苗对BV系病毒引起的流感能起到积极的预防作用。
Objective To understand the characteristics and variation of hemagglutinin(HA1) gene of influenza B virus Victoria lineage in Fengtai District of Beijing in 2017. Methods The HA1 gene of 6 isolated influenza BV outbreak cases were extracted, amplified and sequenced. And sequences were analyzed by bioinformatics software such as DNAStar 7.1,Bioedit v7.0.9, and Mega 6.0.6. Results Among the 6 samples, the 1 st sample was closely related(99.6%) to some Japanese epidemic strains in 2017; the other 5 samples were closely related to the 2016 Pandemic strains of Xiamen, Thailand and Kenya(99.1%-99.8%). The homology of 6 samples with the WHO-recommended vaccine strain B/Brisbane/60/2008 was98.6%. A total of 7 sites were mutated in 6 samples, and there were 5 samples(5/6) had mutations occurred in I132 V,N144 D, and E200 D, which involved the three regions of antigenic determinants A, B, and D, respectively. Conclusion The HA1 gene of BV strains in the Fengtai District of Beijing had been mutated at individual sites, but the matching degree with the WHO-recommended vaccine strain B/Brisbane/60/2008 is relatively high. So continued use of the vaccine against the BV strain can play a positive preventive effect.
Objective To understand the characteristics and variation of hemagglutinin(HA1) gene of influenza B virus Victoria lineage in Fengtai District of Beijing in 2017. Methods The HA1 gene of 6 isolated influenza BV outbreak cases were extracted, amplified and sequenced. And sequences were analyzed by bioinformatics software such as DNAStar 7.1,Bioedit v7.0.9, and Mega 6.0.6. Results Among the 6 samples, the 1 st sample was closely related(99.6%) to some Japanese epidemic strains in 2017; the other 5 samples were closely related to the 2016 Pandemic strains of Xiamen, Thailand and Kenya(99.1%-99.8%). The homology of 6 samples with the WHO-recommended vaccine strain B/Brisbane/60/2008 was98.6%. A total of 7 sites were mutated in 6 samples, and there were 5 samples(5/6) had mutations occurred in I132 V,N144 D, and E200 D, which involved the three regions of antigenic determinants A, B, and D, respectively. Conclusion The HA1 gene of BV strains in the Fengtai District of Beijing had been mutated at individual sites, but the matching degree with the WHO-recommended vaccine strain B/Brisbane/60/2008 is relatively high. So continued use of the vaccine against the BV strain can play a positive preventive effect.
引文
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[2] Hannoun C. The evolving history of influenza viruses and influenza vaccines[J]. Expert Review of Vaccines, 2013, 12(9):1085-1094.
[3] Nerome R, Hiromoto Y, Sugita S, et al. Evolutionary characteristics of influenza B virus since its first isolation in 1940:dynamic circulation of deletion and insertion mechanism[J]. Archives of Virology, 1998,143(8):1569-1583.
[4] Rota PA, Wallis TR, Harmon MW, et al. Cocirculation of two distinct evolutionary lineages of influenza type B virus since 1983[J]. Virology, 1990, 175(1):59-68.
[5] Kanegae Y, Sugita S, Endo A, et al. Evolutionary pattern of the hemagglutinin gene of influenza B viruses isolated in Japan:cocirculating lineages in the same epidemic season[J]. Journal of Virology,1990, 64(6):2860-2865.
[6] Luo C, Morishita T, Satou K, et al. Evolutionary pattern of influenza B viruses based on the HA and NS genes during 1940 to 1999:origin of the NS genes after 1997[J]. Archives of Virology, 1999, 144(10):1881-1891.
[7]郭元吉,程小雯.流行性感冒病毒及其实验技术[M].北京:中国三峡出版社,1997:11-431.
[8]李伟,罗鹏飞,邓斐,等.南京市2011年乙型流感血凝素基因分子特征分析[J].微生物学通报,2013,40(6):1080-1086.
[9] Wang Q, Cheng F, Lu M, et al. Crystal structure of unliganded influenza B virus hemagglutinin[J]. Journal of Virology, 2008, 82(6):3011-3020.
[10]祁贤,崔仑标,李亮,等.江苏首例甲型H1N1(2009)流感病毒分离及其血凝素分子遗传特征分析[J].微生物学通报,2010,37(2):305-310.
[11]杨星钰.基于流感病毒HA2蛋白通用型疫苗的初步研究[D].北京:中国疾病预防控制中心,2012.
[12]李希妍,成艳辉,谭敏菊,等.2012-2013流感监测年度中国大陆B型流感病毒抗原性及基因特性分析[J].疾病监测,2014,29(1):14-20.
[13]李希妍,成艳辉,谭敏菊,等.2014-2015监测年度中国B型流感病毒病原学特征分析[J].中华微生物学和免疫学杂志,2016,36(1):9-15.
[14] Saiki RK, Gelfand DH, Stoffel S, et al. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase[J]. Science(New York, N.Y.), 1988, 239(4839):487-491.
[15] Rivera K, Thomas H, Zhang H, et al. Probing the structure of influenza B hemagglutinin using site-directed mutagenesis[J]. Virology, 1995, 206(2):787-795.