良恶性胸腔积液中lncRNA MEG3和HOTAIR的表达及其鉴别诊断
摘要
目的:探讨良恶性胸腔积液中长链非编码RNA (long non-coding RNA,lncRNA) HOX转录反义RNA(HOX transcript antisense RNA,HOTAIR)和母系表达基因3 (materally expressed gene 3,MEG3)的表达及其临床意义。方法:留取51例恶性胸腔积液患者及50例良性胸腔积液患者的胸腔积液标本,采用实时荧光定量PCR测定HOTAIR和MEG3相对表达水平。通过构建受试者工作特征(ROC)曲线评价这两个lncRNAs甄别良恶性胸腔积液的效能,并与癌胚抗原测定结果比较。结果:恶性胸腔积液中HOTAIR表达水平明显高于良性胸腔积液(t=3. 928,P <0. 05),MEG3表达水平明显低于良性胸腔积液(t=5. 515,P <0. 05)。HOTAIR和MEG3诊断恶性胸液的ROC曲线下面积(AUC)分别为0. 863(95%CI:0. 780～0. 946)和0. 853(95%CI:0. 766～0. 940),与癌胚抗原AUC(0. 850,95%CI:0. 764～0. 936)相近。HOTAIR和MEG3诊断恶性胸腔积液的敏感性分别为85. 8%和87. 9%,特异性分别为84. 1%和88. 1%,略高于癌胚抗原的敏感性(74. 0%)和特异性(80. 2%)。联合检测胸腔积液中HOTAIR、MEG3和癌胚抗原可进一步提高诊断恶性胸腔积液的敏感性(90. 0%)和特异性(93. 1%)。结论:良恶性胸腔积液中HOTAIR和MEG3表达有差异,两者检测对胸腔积液的鉴别诊断有一定临床价值。
引文
[1]Desai NR,Lee HJ.Diagnosis and management of malignant pleural effusions:state of the art in 2017[J].JThorac Dis,2017,9(10):S1111-S1122.
[2]Ono H,Motoi N,Nagano H,et al.Long noncoding RNA HOTAIR is relevant to cellular proliferation,invasiveness,and clinical relapse in small-cell lung cancer[J].Cancer Med,2014,3(3):632-642.
[3]Liu XH,Liu ZL,Sun M,et al.The long non-coding RNA HOTAIR indicates a poor prognosis and promotes metastasis in non-small cell lung cancer[J].BMCCancer,2013,13:464.
[4]Lu KH,Li W,Liu XH,et al.Long non-coding RNAMEG3 inhibits NSCLC cells proliferation and induces apoptosis by affecting p53 expression[J].BMC Cancer,2013,13:461.
[5]Kruer TL,Dougherty SM,Reynolds L,et al.Expression of the lncRNA maternally expressed gene 3(MEG3)contributes to the control of lung cancer cell proliferation by the Rb pathway[J].PLo S One,2016,11(11):e0166363.
[6]He Y,Luo Y,Liang B,et al.Potential applications of MEG3 in cancer diagnosis and prognosis[J].Oncotarget,2017,8(42):73282-73295.
[7]Hajjari M,Salavaty A.HOTAIR:an oncogenic long non-coding RNA in different cancers[J].Cancer Biol Med,2015,12(1):1-9.
[8]Ahadi M,Tehranian S,Memar B,et al.Diagnostic value of carcinoembryonic antigen in malignancy-related ascites:systematic review and meta-analysis[J].Acta Gastroenterol Belg,2014,77(4):418-424.
[9]薛秋婷,顾兵,朱立强.液基细胞学联合免疫细胞化学对疑难肺腺癌转移胸腔积液的诊断价值[J].临床与病理杂志,2016,36(12):1938-1943.
[10]Gu Y,Zhai K,Shi HZ.Clinical value of tumor markers for determining cause of pleural effusion[J].Chin Med J,2016,129(3):253-258.
[11]Prabhu VG,Narasimhan R.The role of pleuroscopy in undiagnosed exudative pleural effusion[J].Lung India,2012,29(2):128-130.
[12]Antonangelo L,Sales RK,Cor AP,et al.Pleural fluid tumour markers in malignant pleural effusion with inconclusive cytologic results[J].Curr Oncol,2015,22(5):e336-e341.
[13]Liang W,Lv T,Shi X,et al.Circulating long noncoding RNA GAS5 is a novel biomarker for the diagnosis of nonsmall cell lung cancer[J].Medicine(Baltimore),2016,95(37):e4608.
[14]谭楠,李莉红,白璐,等.非小细胞肺癌血清中lncRNA HOTAIR的表达及临床意义[J].中国肺癌杂志,2017,20(6):402-406.
[15]Hooper C,Lee YC,Maskell N,et al.Investigation of a unilateral pleural effusion in adults:British Thoracic Disease Guideline 2010[J].Thorax,2010,65(Suppl2):ii4-ii17.
[2]Ono H,Motoi N,Nagano H,et al.Long noncoding RNA HOTAIR is relevant to cellular proliferation,invasiveness,and clinical relapse in small-cell lung cancer[J].Cancer Med,2014,3(3):632-642.
[3]Liu XH,Liu ZL,Sun M,et al.The long non-coding RNA HOTAIR indicates a poor prognosis and promotes metastasis in non-small cell lung cancer[J].BMCCancer,2013,13:464.
[4]Lu KH,Li W,Liu XH,et al.Long non-coding RNAMEG3 inhibits NSCLC cells proliferation and induces apoptosis by affecting p53 expression[J].BMC Cancer,2013,13:461.
[5]Kruer TL,Dougherty SM,Reynolds L,et al.Expression of the lncRNA maternally expressed gene 3(MEG3)contributes to the control of lung cancer cell proliferation by the Rb pathway[J].PLo S One,2016,11(11):e0166363.
[6]He Y,Luo Y,Liang B,et al.Potential applications of MEG3 in cancer diagnosis and prognosis[J].Oncotarget,2017,8(42):73282-73295.
[7]Hajjari M,Salavaty A.HOTAIR:an oncogenic long non-coding RNA in different cancers[J].Cancer Biol Med,2015,12(1):1-9.
[8]Ahadi M,Tehranian S,Memar B,et al.Diagnostic value of carcinoembryonic antigen in malignancy-related ascites:systematic review and meta-analysis[J].Acta Gastroenterol Belg,2014,77(4):418-424.
[9]薛秋婷,顾兵,朱立强.液基细胞学联合免疫细胞化学对疑难肺腺癌转移胸腔积液的诊断价值[J].临床与病理杂志,2016,36(12):1938-1943.
[10]Gu Y,Zhai K,Shi HZ.Clinical value of tumor markers for determining cause of pleural effusion[J].Chin Med J,2016,129(3):253-258.
[11]Prabhu VG,Narasimhan R.The role of pleuroscopy in undiagnosed exudative pleural effusion[J].Lung India,2012,29(2):128-130.
[12]Antonangelo L,Sales RK,Cor AP,et al.Pleural fluid tumour markers in malignant pleural effusion with inconclusive cytologic results[J].Curr Oncol,2015,22(5):e336-e341.
[13]Liang W,Lv T,Shi X,et al.Circulating long noncoding RNA GAS5 is a novel biomarker for the diagnosis of nonsmall cell lung cancer[J].Medicine(Baltimore),2016,95(37):e4608.
[14]谭楠,李莉红,白璐,等.非小细胞肺癌血清中lncRNA HOTAIR的表达及临床意义[J].中国肺癌杂志,2017,20(6):402-406.
[15]Hooper C,Lee YC,Maskell N,et al.Investigation of a unilateral pleural effusion in adults:British Thoracic Disease Guideline 2010[J].Thorax,2010,65(Suppl2):ii4-ii17.