48株爱德华氏菌糖酵解持家酶的胞外分泌
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摘要
【背景】病原菌的糖酵解持家酶能分泌到胞外或定位在细胞膜表面,在病原菌的侵染和细胞粘附方面发挥着重要作用,爱德华氏菌是重要的鱼类致病菌,研究其糖酵解持家酶的胞外分泌有助于该病原的致病机制研究和疫苗开发。【目的】探究爱德华氏菌中糖酵解持家酶的胞外分泌。【方法】通过ELISA方法考察48种不同来源的爱德华氏菌中5种糖酵持家酶的胞外分泌。【结果】48种不同来源的爱德华氏菌中糖酵解持家酶蛋白均能分泌到胞外。【结论】爱德华氏菌中糖酵解持家酶的胞外分泌是普遍现象。
[Background] The glycolytic housekeeping enzyme can be secreted to the extracellular matrix or located on the surface of the cell membrane, which plays an important role in the infection and cell adhesion of pathogen. The study of the secretion of glycolytic housekeeping enzymes in Edwardsiella, which is an important fish pathogen, is benefit to its pathogenesis and vaccine development. [Objective] To explore the secretion of housekeeping enzymes in glycolysis of Edwardsiella. [Methods] With ELISA, five housekeeping enzymes in the extracellular proteins from 48 different strains of of Edwardsiella was investigated. [Results] Five glycolytic enzymes are widespread in the extracellular protein of 48 strains of Edwardsiella. [Conclusion] Extracellular secretion of glycolytic enzymes in Edwardsiella is a common phenomenon.
[Background] The glycolytic housekeeping enzyme can be secreted to the extracellular matrix or located on the surface of the cell membrane, which plays an important role in the infection and cell adhesion of pathogen. The study of the secretion of glycolytic housekeeping enzymes in Edwardsiella, which is an important fish pathogen, is benefit to its pathogenesis and vaccine development. [Objective] To explore the secretion of housekeeping enzymes in glycolysis of Edwardsiella. [Methods] With ELISA, five housekeeping enzymes in the extracellular proteins from 48 different strains of of Edwardsiella was investigated. [Results] Five glycolytic enzymes are widespread in the extracellular protein of 48 strains of Edwardsiella. [Conclusion] Extracellular secretion of glycolytic enzymes in Edwardsiella is a common phenomenon.
引文
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[13]Sun ZY,Shen BB,Wu HZ,et al.The secreted fructose1,6-bisphosphate aldolase as a broad spectrum vaccine candidate against pathogenic bacteria in aquaculture[J].Fish&Shellfish Immunology,2015,46(2):638-647
[14]Liu XH,Sun JM,Wu HZ.Glycolysis-related proteins are broad spectrum vaccine candidates against aquacultural pathogens[J].Vaccine,2017,35(31):3813-3816
[15]Wang YM,Wang QY,Xiao JF,et al.Genetic relationships of Edwardsiella strains isolated in China aquaculture revealed by rep-PCR genomic fingerprinting and investigation of Edwardsiella virulence genes[J].Journal of Applied Microbiology,2011,111(6):1337-1348
[16]Terao Y,Yamaguchi M,Hamada S,et al.Multifunctional glyceraldehyde-3-phosphate dehydrogenase of Streptococcus pyogenes is essential for evasion from neutrophils[J].Journal of Biological Chemistry,2006,281(20):14215-14223
[17]Egea L,Aguilera L,Giménez R,et al.Role of secreted glyceraldehyde-3-phosphate dehydrogenase in the infection mechanism of enterohemorrhagic and enteropathogenic Escherichia coli:interaction of the extracellular enzyme with human plasminogen and fibrinogen[J].The International Journal of Biochemistry&Cell Biology,2007,39(6):1190-1203
[18]Alves J,Madureira P,Baltazar MT,et al.A safe and stable neonatal vaccine targeting GAPDH confers protection against Group B streptococcus infections in adult susceptible mice[J].PLoS One,2015,10(12):e0144196
[19]Oliveira L,Madureira P,Andrade EB,et al.Group Bstreptococcus GAPDH is released upon cell lysis,associates with bacterial surface,and induces apoptosis in murine macrophages[J].PLoS One,2012,7(1):e29963
[20]Gómez-Arreaza A,Acosta H,Qui?ones W,et al.Extracellular functions of glycolytic enzymes of parasites:unpredicted use of ancient proteins[J].Molecular and Biochemical Parasitology,2014,193(2):75-81
[21]Tunio SA,Oldfield NJ,Berry A,et al.The moonlighting protein fructose-1,6-bisphosphate aldolase of Neisseria meningitidis:surface localization and role in host cell adhesion[J].Molecular Microbiology,2010,76(3):605-615
[22]Yamaguchi M,Ikeda R,Nishimura M,et al.Localization by scanning immunoelectron microscopy of triosephosphate isomerase,the molecules responsible for contact-mediated killing of Cryptococcus,on the surface of Staphylococcus[J].Microbiology and Immunology,2010,54(6):368-370
[23]Sha J,Erova TE,Alyea RA,et al.Surface-expressed enolase contributes to the pathogenesis of clinical isolate SSU of Aeromonas hydrophila[J].Journal of Bacteriology,2009,191(9):3095-3107
[24]Antúnez K,Anido M,Arredondo D,et al.Paenibacillus larvae enolase as a virulence factor in honeybee larvae infection[J].Veterinary Microbiology,2011,147(1/2):83-89
[25]Deng J,Wu HZ.Regulation of GAPDH secretion in Edwardsiella tarda[J].Microbiology China,2017,44(10):2398-2406(in Chinese)邓佳,吴海珍.迟缓爱德华氏菌中甘油醛-3-磷酸脱氢酶的胞外分泌调控[J].微生物学通报,2017,44(10):2398-2406
[2]Henderson B.An overview of protein moonlighting in bacterial infection[J].Biochemical Society Transactions,2014,42(6):1720-1727
[3]Perez-Casal J,Potter AA.Glyceradehyde-3-phosphate dehydrogenase as a suitable vaccine candidate for protection against bacterial and parasitic diseases[J].Vaccine,2016,34(8):1012-1017
[4]McCarthy JS,Wieseman M,Tropea J,et al.Onchocerca volvulus glycolytic enzyme fructose-1,6-bisphosphate aldolase as a target for a protective immune response in humans[J].Infection and Immunity,2002,70(2):851-858
[5]Marques HH,Zouain CS,Torres CBB,et al.Protective effect and granuloma down-modulation promoted by RP44antigen a fructose 1,6 bisphosphate aldolase of Schistosoma mansoni[J].Immunobiology,2008,213(5):437-446
[6]Ling E,Feldman G,Portnoi M,et al.Glycolytic enzymes associated with the cell surface of Streptococcus pneumoniae are antigenic in humans and elicit protective immune responses in the mouse[J].Clinical&Experimental Immunology,2004,138(2):290-298
[7]Hewitson JP,Rückerl D,Harcus Y,et al.The secreted triose phosphate isomerase of Brugia malayi is required to sustain microfilaria production in vivo[J].PLoS Pathogen,2014,10(2):e1003930
[8]Calcedo R,Ramirez-Garcia A,Abad A,et al.Phosphoglycerate kinase and fructose bisphosphate aldolase of Candida albicans as new antigens recognized by human salivary IgA[J].Revista Iberoamericana de Micologia,2012,29(3):172-174
[9]Veiga-Malta I,Duarte M,Dinis M,et al.Enolase from Streptococcus sobrinus is an immunosuppressive protein[J].Cellular Microbiology,2004,6(1):79-88
[10]Dinis M,Tavares D,Veiga-Malta I,et al.Oral therapeutic vaccination with Streptococcus sobrinus recombinant enolase confers protection against dental caries in rats[J].The Journal of Infectious Disease,2009,199(1):116-123
[11]Li X,Wu H,Zhang M,et al.Secreted glyceraldehyde-3-phosphate dehydrogenase as a broad spectrum vaccine candidate against microbial infection in aquaculture[J].Letters of Applied Microbiology,2012,54(1):1-9
[12]Liang SQ,Wu HZ,Liu B,et al.Immune response of turbot(Scophthalmus maximus L.)to a broad spectrum vaccine candidate,recombinant glyceraldehyde-3-phosphate dehydrogenase of Edwardsiella tarda[J].Veterinary Immunology and Immunopathology,2012,150(3/4):198-205
[13]Sun ZY,Shen BB,Wu HZ,et al.The secreted fructose1,6-bisphosphate aldolase as a broad spectrum vaccine candidate against pathogenic bacteria in aquaculture[J].Fish&Shellfish Immunology,2015,46(2):638-647
[14]Liu XH,Sun JM,Wu HZ.Glycolysis-related proteins are broad spectrum vaccine candidates against aquacultural pathogens[J].Vaccine,2017,35(31):3813-3816
[15]Wang YM,Wang QY,Xiao JF,et al.Genetic relationships of Edwardsiella strains isolated in China aquaculture revealed by rep-PCR genomic fingerprinting and investigation of Edwardsiella virulence genes[J].Journal of Applied Microbiology,2011,111(6):1337-1348
[16]Terao Y,Yamaguchi M,Hamada S,et al.Multifunctional glyceraldehyde-3-phosphate dehydrogenase of Streptococcus pyogenes is essential for evasion from neutrophils[J].Journal of Biological Chemistry,2006,281(20):14215-14223
[17]Egea L,Aguilera L,Giménez R,et al.Role of secreted glyceraldehyde-3-phosphate dehydrogenase in the infection mechanism of enterohemorrhagic and enteropathogenic Escherichia coli:interaction of the extracellular enzyme with human plasminogen and fibrinogen[J].The International Journal of Biochemistry&Cell Biology,2007,39(6):1190-1203
[18]Alves J,Madureira P,Baltazar MT,et al.A safe and stable neonatal vaccine targeting GAPDH confers protection against Group B streptococcus infections in adult susceptible mice[J].PLoS One,2015,10(12):e0144196
[19]Oliveira L,Madureira P,Andrade EB,et al.Group Bstreptococcus GAPDH is released upon cell lysis,associates with bacterial surface,and induces apoptosis in murine macrophages[J].PLoS One,2012,7(1):e29963
[20]Gómez-Arreaza A,Acosta H,Qui?ones W,et al.Extracellular functions of glycolytic enzymes of parasites:unpredicted use of ancient proteins[J].Molecular and Biochemical Parasitology,2014,193(2):75-81
[21]Tunio SA,Oldfield NJ,Berry A,et al.The moonlighting protein fructose-1,6-bisphosphate aldolase of Neisseria meningitidis:surface localization and role in host cell adhesion[J].Molecular Microbiology,2010,76(3):605-615
[22]Yamaguchi M,Ikeda R,Nishimura M,et al.Localization by scanning immunoelectron microscopy of triosephosphate isomerase,the molecules responsible for contact-mediated killing of Cryptococcus,on the surface of Staphylococcus[J].Microbiology and Immunology,2010,54(6):368-370
[23]Sha J,Erova TE,Alyea RA,et al.Surface-expressed enolase contributes to the pathogenesis of clinical isolate SSU of Aeromonas hydrophila[J].Journal of Bacteriology,2009,191(9):3095-3107
[24]Antúnez K,Anido M,Arredondo D,et al.Paenibacillus larvae enolase as a virulence factor in honeybee larvae infection[J].Veterinary Microbiology,2011,147(1/2):83-89
[25]Deng J,Wu HZ.Regulation of GAPDH secretion in Edwardsiella tarda[J].Microbiology China,2017,44(10):2398-2406(in Chinese)邓佳,吴海珍.迟缓爱德华氏菌中甘油醛-3-磷酸脱氢酶的胞外分泌调控[J].微生物学通报,2017,44(10):2398-2406