萎凋对茶鲜叶不同叶位叶绿素荧光特征参数的影响
|
摘要
目的研究萎凋对茶叶水分和叶绿素荧光参数的影响。方法采用叶绿素荧光仪Imaging PAM对福云6号7个萎凋时间点进行水分含量测定及叶绿素荧光测定。结果不同叶位叶片萎凋过程的水分含量与叶绿素荧光参数之间具有一定的内在联系。不同叶位之间的叶绿素荧光参数在萎凋后期呈显著差异;叶片水分含量与叶绿素荧光参数的初始荧光F_0、非光化学淬灭系数(non-photochemical quenching, NPQ)之间呈极显著负相关,与叶绿素荧光参数的最大光化学效率F_v/F_m、光合潜在活性F_v/F_0、电子传递效率(electrontransferrate,ETR)、实际光合量子产量Y(Ⅱ)之间呈极显著正相关。结论鉴于上述叶绿素荧光参数与叶片水分含量之间的相关性,初步认为F_0、NPQ、F_v/F_m、F_v/F_0、ETR和Y(Ⅱ)作为茶树鲜叶萎凋过程中水分胁迫程度的指标是可行的,对鲜叶萎凋过程的在线判断具有一定的意义。
Objective To study the effects of withering on tea moisture and chlorophyll fluorescence parameters. Methods The chlorophyll fluorescence instrument Imaging PAM was used to determine the moisture content and chlorophyll fluorescence of the 7 withering time points of Fuyun no. 6. Results There was an internal relationship between the water content and chlorophyll fluorescence parameters of the leaf wilting process in different leaf positions. Chlorophyll fluorescence parameters in different leaf positions showed significantly different at the later stage of withering. There was a significant negative correlation between leaf water content and initial fluorescence F_0 and non-photochemical quenching(NPQ) of chlorophyll fluorescence parameters. And there was a significant positive correlation between leaf moisture content and chlorophyll fluorescence parameters, maximum photochemical efficiency F_v/F_m, photosynthetic potential activity F_v/F_0, electron transfer rate(ETR), and actual photosynthetic quantum yield Y(Ⅱ). Conclusion In view of the correlation between chlorophyll fluorescence parameters mentioned above and leaf moisture content, it is preliminarily considered that F_0, NPQ, F_v/F_m, F_v/F_0, ETR and Y(Ⅱ) are feasible indicators of water stress degree during the withering process of fresh leaves of tea trees, which has certain significance for online judgment of the withering process of fresh leaves.
Objective To study the effects of withering on tea moisture and chlorophyll fluorescence parameters. Methods The chlorophyll fluorescence instrument Imaging PAM was used to determine the moisture content and chlorophyll fluorescence of the 7 withering time points of Fuyun no. 6. Results There was an internal relationship between the water content and chlorophyll fluorescence parameters of the leaf wilting process in different leaf positions. Chlorophyll fluorescence parameters in different leaf positions showed significantly different at the later stage of withering. There was a significant negative correlation between leaf water content and initial fluorescence F_0 and non-photochemical quenching(NPQ) of chlorophyll fluorescence parameters. And there was a significant positive correlation between leaf moisture content and chlorophyll fluorescence parameters, maximum photochemical efficiency F_v/F_m, photosynthetic potential activity F_v/F_0, electron transfer rate(ETR), and actual photosynthetic quantum yield Y(Ⅱ). Conclusion In view of the correlation between chlorophyll fluorescence parameters mentioned above and leaf moisture content, it is preliminarily considered that F_0, NPQ, F_v/F_m, F_v/F_0, ETR and Y(Ⅱ) are feasible indicators of water stress degree during the withering process of fresh leaves of tea trees, which has certain significance for online judgment of the withering process of fresh leaves.
引文
[1]王丽丽,宋振硕,陈键,等.茶鲜叶萎凋过程中儿茶素和生物碱的动态变化规律[J].福建农业学报,2015,30(9):856-862.Wang LL,Song ZS,Chen J,et al.Dynamic changes of catechins and alkaloids in the process of withering leaves of fresh tea leaves[J].Fujian JAgric Sci,2015,30(9):856-862.
[2]吴久赟,廉苇佳,刘志刚,等.不同葡萄品种叶绿素荧光参数的高温响应及其耐热性评价[J].西北农林科技大学学报(自然科学版),2019,(6):1-8.Wu JY,Lian WJ,Liu ZG,et al.Evaluation of high temperature response and heat tolerance of chlorophyll fluorescence parameters of different grape varieties[J].J Northwest A&F Univ(Nat Sci Ed),2019,(6):1-8
[3]段娜,贾玉奎,郝玉光,等.干旱胁迫对欧李叶绿素荧光特性的影响[J].西北林学院学报,2018,33(6):10-14.Duan N,Jia YK,Hao YG,et al.Effects of drought stress on chlorophyll fluorescence characteristics of eucalyptus[J].J Northwest For Univ,2018,33(6):10-14.
[4]Rei S,Quan W.Assessing hyperspectral indices for tracing chlorophyll fluorescence parameters in deciduous forests[J].J Environ Manag,2018,85(6):172-180.
[5]Yan YY,Yang B,Lan XY,et al.Cadmium accumulation capacity and resistance strategies of cadmium-hypertolerant fern-Microsorum fortune[J].Sci Total Environ,2018,(649):1209-1223.
[6]白志英,李存东,赵金锋,等.干旱胁迫对小麦代换系叶绿素荧光参数的影响及染色体效应初步分析[J].中国农业科学,2011,44(1):47-57.Bai ZY,Li CD,Zhao JF,et al.Effects of drought stress on chlorophyll fluorescence parameters of wheat substitution lines and preliminary analysis of chromosomal effects[J].Sci Agric Sin,2011,44(1):47-57.
[7]Semerci A,?i?ek N,Karahan F,et al.Some growth and chlorophyll fluorescence parameters of black and hybrid poplar clones under water stress[J].Turkish J Agric For,2017,41(5):348-356.
[8]王莹博,许申平,马杰,等.高温干旱胁迫对白及光合特性及叶绿素荧光参数的影响[J].河南农业大学学报,2018,52(2):199-205.Wang YB,Xu SP,Ma J,et al.Effects of high temperature and drought stress on whitening,photosynthetic characteristics and chlorophyll fluorescence parameters[J].J Henan Agric Univ,2018,52(2):199-205.
[9]孙敏,李树斌,唐飘,等.干旱胁迫对杉木无性系叶绿素荧光特性的影响[J].森林与环境学报,2018,38(2):202-208.Sun M,Li SB,Tang P,et al.Effects of drought stress on chlorophyll fluorescence characteristics of Chinese fir clones[J].J For Environ,2018,38(2):202-208.
[10]孙志勇,季孔庶.干旱胁迫对4个杂交鹅掌楸无性系叶绿素荧光特性的影响[J].西北林学院学报,2010,25(4):35-39.Sun ZY,Ji KS.Effects of drought stress on chlorophyll fluorescence characteristics of four hybrid liriodendron clones[J].J Northwest For Univ,2010,25(4):35-39.
[11]Zhang Y,Shi Y,Gong HJ,et al.Beneficial effects of silicon on photosynthesis of tomato seedlings under water stress[J].J Integr Agric,2018,30(10):2151-2159.
[12]Gao ML,Liu Y,Dong YM,et al.Physiological responses of wheat planted in fluvo-aquic soils to di(2-ethylhexyl)and di-n-butyl phthalates[J].Environ Poll,2018,(1):774-782.
[13]Yang ZX,Yang YF,Yu SZ,et al.Photosynthetic,photochemical and osmotic regulation changes in tobacco resistant and susceptible to Alternaria alternate[J].Trop Plant Pathol,2018,43(5):413-421.
[14]Bj?rkman O,Demmig B.Photon yield of O2 evolution and chlorophyll fluorescence characteristics at 77 K among vascular plants of diverse origins[J].Planta,1987,170(4):489-504.
[15]?quist G,Anderson JM,McCaffery S,et al.Mechanistic differences in photoinhibition of sun and shade plants[J].Planta,1992,188(3):422-431.
[16]郭春芳,孙云,唐玉海,等.水分胁迫对茶树叶片叶绿素荧光特性的影响[J].中国生态农业学报,2009,17(3):560-564.Guo CF,Sun Y,Tang YH,et al.Effects of water stress on chlorophyll fluorescence characteristics of tea leaves[J].Chin J Eco-Agric,2009,17(3):560-564.
[17]王雯,李曼,王丽红,等.酸雨对全生育时期水稻叶绿素荧光的影响[J].生态环境学报,2014,23(1):80-85.Wang W,Li M,Wang LH,et al.Effects of acid rain on chlorophyll fluorescence of rice during whole growth period[J].Chin J Eco-Environ,2014,23(1):80-85.
[18]Fagundes-Nacarath IRF,Debona D,Brás VV,et al.Rodrigues phosphites attenuate sclerotinia sclerotiorum-induced physiological impairments in common bean[J].Acta Physiolog Plantarum,2018,(137):1664-1681.
[19]段东平,徐炳成,牛富荣,等.水分和磷肥对达乌里胡枝子不同叶位叶绿素荧光参数特征的影响[J].草业科学,2012,29(3):422-428.Duan DP,Xu BC,Niu FR,et al.Effects of water and phosphorus fertilizers on chlorophyll fluorescence parameters in different leaf positions of D.Chinensis[J].Grass Sci,2012,29(3):422-428.
[20]徐建伟,席万鹏,方憬军,等.水分胁迫对葡萄叶绿素荧光参数的影响[J].西北农业学报,2007,(5):175-179.Xu JW,Xi WP,Fang JJ,et al.Effects of water stress on chlorophyll fluorescence parameters of grape[J].Northwest Agric J,2007,(5):175-179.
[21]何溪宏,张伟.棉花幼苗叶绿素荧光特性对低温胁迫的响应研究[J].新疆农垦科技,2015,38(6):56-57.He XH,Zhang W.Response of chlorophyll fluorescence characteristics of cotton seedlings to low temperature stress[J].Xinjiang Agric Reclam Sci Technol,2015,38(6):56-57.
[22]余凌翔,朱勇,钟楚,等.生态环境对烟草农艺性状和叶绿素荧光特性的影响[J].中国农业气象,2015,36(2):149-154.Yu LX,Zhu Y,Zhong C,et al.Effects of ecological environment on tobacco agronomic traits and chlorophyll fluorescence characteristics[J].Chin J Agrometeorol,2015,36(2):149-154.
[23]Genty B,Briantais JM,Baker NR.The relationship between quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence[J].Biochim Biophys Acta,1989,990(1):87-92.
[24]Gharbi F,Zribi L,Daly AB,et al.Photosynthetic responses of tomato leaves to salt and cadmium stresses:growth and chlorophyll a fluorescence kinetic analyses[J].Polish J Environ Stud,2018,27(6):1-10.
[25]Ornusa K,Weerasin S,Srisom S,et al.Effects of 24-epibrassinolide and the synthetic brassinosteroid mimic on chili pepper under drought[J].ACTA Phys Plant Acta Phys Plant,2018,(137):1861-1864.
[26]Hazrati S,Tahmasebi S.Effects of water stress and light intensity on chlorophyll fluorescence parameters and pigments of Aloe vera L[J].Plant Phys Biochem,2016,(106):141-148.
[2]吴久赟,廉苇佳,刘志刚,等.不同葡萄品种叶绿素荧光参数的高温响应及其耐热性评价[J].西北农林科技大学学报(自然科学版),2019,(6):1-8.Wu JY,Lian WJ,Liu ZG,et al.Evaluation of high temperature response and heat tolerance of chlorophyll fluorescence parameters of different grape varieties[J].J Northwest A&F Univ(Nat Sci Ed),2019,(6):1-8
[3]段娜,贾玉奎,郝玉光,等.干旱胁迫对欧李叶绿素荧光特性的影响[J].西北林学院学报,2018,33(6):10-14.Duan N,Jia YK,Hao YG,et al.Effects of drought stress on chlorophyll fluorescence characteristics of eucalyptus[J].J Northwest For Univ,2018,33(6):10-14.
[4]Rei S,Quan W.Assessing hyperspectral indices for tracing chlorophyll fluorescence parameters in deciduous forests[J].J Environ Manag,2018,85(6):172-180.
[5]Yan YY,Yang B,Lan XY,et al.Cadmium accumulation capacity and resistance strategies of cadmium-hypertolerant fern-Microsorum fortune[J].Sci Total Environ,2018,(649):1209-1223.
[6]白志英,李存东,赵金锋,等.干旱胁迫对小麦代换系叶绿素荧光参数的影响及染色体效应初步分析[J].中国农业科学,2011,44(1):47-57.Bai ZY,Li CD,Zhao JF,et al.Effects of drought stress on chlorophyll fluorescence parameters of wheat substitution lines and preliminary analysis of chromosomal effects[J].Sci Agric Sin,2011,44(1):47-57.
[7]Semerci A,?i?ek N,Karahan F,et al.Some growth and chlorophyll fluorescence parameters of black and hybrid poplar clones under water stress[J].Turkish J Agric For,2017,41(5):348-356.
[8]王莹博,许申平,马杰,等.高温干旱胁迫对白及光合特性及叶绿素荧光参数的影响[J].河南农业大学学报,2018,52(2):199-205.Wang YB,Xu SP,Ma J,et al.Effects of high temperature and drought stress on whitening,photosynthetic characteristics and chlorophyll fluorescence parameters[J].J Henan Agric Univ,2018,52(2):199-205.
[9]孙敏,李树斌,唐飘,等.干旱胁迫对杉木无性系叶绿素荧光特性的影响[J].森林与环境学报,2018,38(2):202-208.Sun M,Li SB,Tang P,et al.Effects of drought stress on chlorophyll fluorescence characteristics of Chinese fir clones[J].J For Environ,2018,38(2):202-208.
[10]孙志勇,季孔庶.干旱胁迫对4个杂交鹅掌楸无性系叶绿素荧光特性的影响[J].西北林学院学报,2010,25(4):35-39.Sun ZY,Ji KS.Effects of drought stress on chlorophyll fluorescence characteristics of four hybrid liriodendron clones[J].J Northwest For Univ,2010,25(4):35-39.
[11]Zhang Y,Shi Y,Gong HJ,et al.Beneficial effects of silicon on photosynthesis of tomato seedlings under water stress[J].J Integr Agric,2018,30(10):2151-2159.
[12]Gao ML,Liu Y,Dong YM,et al.Physiological responses of wheat planted in fluvo-aquic soils to di(2-ethylhexyl)and di-n-butyl phthalates[J].Environ Poll,2018,(1):774-782.
[13]Yang ZX,Yang YF,Yu SZ,et al.Photosynthetic,photochemical and osmotic regulation changes in tobacco resistant and susceptible to Alternaria alternate[J].Trop Plant Pathol,2018,43(5):413-421.
[14]Bj?rkman O,Demmig B.Photon yield of O2 evolution and chlorophyll fluorescence characteristics at 77 K among vascular plants of diverse origins[J].Planta,1987,170(4):489-504.
[15]?quist G,Anderson JM,McCaffery S,et al.Mechanistic differences in photoinhibition of sun and shade plants[J].Planta,1992,188(3):422-431.
[16]郭春芳,孙云,唐玉海,等.水分胁迫对茶树叶片叶绿素荧光特性的影响[J].中国生态农业学报,2009,17(3):560-564.Guo CF,Sun Y,Tang YH,et al.Effects of water stress on chlorophyll fluorescence characteristics of tea leaves[J].Chin J Eco-Agric,2009,17(3):560-564.
[17]王雯,李曼,王丽红,等.酸雨对全生育时期水稻叶绿素荧光的影响[J].生态环境学报,2014,23(1):80-85.Wang W,Li M,Wang LH,et al.Effects of acid rain on chlorophyll fluorescence of rice during whole growth period[J].Chin J Eco-Environ,2014,23(1):80-85.
[18]Fagundes-Nacarath IRF,Debona D,Brás VV,et al.Rodrigues phosphites attenuate sclerotinia sclerotiorum-induced physiological impairments in common bean[J].Acta Physiolog Plantarum,2018,(137):1664-1681.
[19]段东平,徐炳成,牛富荣,等.水分和磷肥对达乌里胡枝子不同叶位叶绿素荧光参数特征的影响[J].草业科学,2012,29(3):422-428.Duan DP,Xu BC,Niu FR,et al.Effects of water and phosphorus fertilizers on chlorophyll fluorescence parameters in different leaf positions of D.Chinensis[J].Grass Sci,2012,29(3):422-428.
[20]徐建伟,席万鹏,方憬军,等.水分胁迫对葡萄叶绿素荧光参数的影响[J].西北农业学报,2007,(5):175-179.Xu JW,Xi WP,Fang JJ,et al.Effects of water stress on chlorophyll fluorescence parameters of grape[J].Northwest Agric J,2007,(5):175-179.
[21]何溪宏,张伟.棉花幼苗叶绿素荧光特性对低温胁迫的响应研究[J].新疆农垦科技,2015,38(6):56-57.He XH,Zhang W.Response of chlorophyll fluorescence characteristics of cotton seedlings to low temperature stress[J].Xinjiang Agric Reclam Sci Technol,2015,38(6):56-57.
[22]余凌翔,朱勇,钟楚,等.生态环境对烟草农艺性状和叶绿素荧光特性的影响[J].中国农业气象,2015,36(2):149-154.Yu LX,Zhu Y,Zhong C,et al.Effects of ecological environment on tobacco agronomic traits and chlorophyll fluorescence characteristics[J].Chin J Agrometeorol,2015,36(2):149-154.
[23]Genty B,Briantais JM,Baker NR.The relationship between quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence[J].Biochim Biophys Acta,1989,990(1):87-92.
[24]Gharbi F,Zribi L,Daly AB,et al.Photosynthetic responses of tomato leaves to salt and cadmium stresses:growth and chlorophyll a fluorescence kinetic analyses[J].Polish J Environ Stud,2018,27(6):1-10.
[25]Ornusa K,Weerasin S,Srisom S,et al.Effects of 24-epibrassinolide and the synthetic brassinosteroid mimic on chili pepper under drought[J].ACTA Phys Plant Acta Phys Plant,2018,(137):1861-1864.
[26]Hazrati S,Tahmasebi S.Effects of water stress and light intensity on chlorophyll fluorescence parameters and pigments of Aloe vera L[J].Plant Phys Biochem,2016,(106):141-148.