超临界萃取山柚油及其Sn-2位脂肪酸的测定
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摘要
实验以海南山柚为原料,通过单因素正交实验对超临界萃取山柚油的工艺进行优化,以确定最佳的工艺条件,从而为山柚油的萃取以及超临界萃取方法提供理论依据,并采用氨基固相萃取法联用气相色谱测定山柚油中Sn-2位脂肪酸成分和含量。在相关文献以及预实验的基础上,确定了4个单因素,当萃取温度为40℃,萃取压力为25 MPa,萃取流量为20 L/min,萃取时间为90min的条件下,萃取率达到最大,为80.05%。与传统的提取方法相比,它具有提取温度较低,无有机溶剂残留,提取率也较高等优势,所得的山柚油品质较优且Sn-2位油酸和亚油酸含量较高,具有较高的营养价值。
To obtain the optimum supercritical fluid extraction(SFE) technology of Hainan camellia oil, the single-factor extraction tests and orthogonal experiment were employed to optimize the technological conditions, including extraction pressure, temperature, static extraction time, and flow rate. And the composition and content of Sn-2 fatty acids in Hainan camellia oil were determined by gas chromatography with NH2 extraction cartridge. The results indicated that the optimal extraction conditions were as follows: extraction pressure of 25 MPa, extraction temperature of 40 ℃, static extraction time of 90 min and flow rate of 20 L/min. Under the conditions, the highest extraction rate of Hainan camellia oil was 80.05%. Comparing to the traditional extraction, the advantages of the SFE are that extraction temperature is low, no solvent residue, and higher extraction rate. The obtained pomelo oil has better quality and higher content of oleic acid and linoleic acid in Sn-2, which has higher nutritional value.
To obtain the optimum supercritical fluid extraction(SFE) technology of Hainan camellia oil, the single-factor extraction tests and orthogonal experiment were employed to optimize the technological conditions, including extraction pressure, temperature, static extraction time, and flow rate. And the composition and content of Sn-2 fatty acids in Hainan camellia oil were determined by gas chromatography with NH2 extraction cartridge. The results indicated that the optimal extraction conditions were as follows: extraction pressure of 25 MPa, extraction temperature of 40 ℃, static extraction time of 90 min and flow rate of 20 L/min. Under the conditions, the highest extraction rate of Hainan camellia oil was 80.05%. Comparing to the traditional extraction, the advantages of the SFE are that extraction temperature is low, no solvent residue, and higher extraction rate. The obtained pomelo oil has better quality and higher content of oleic acid and linoleic acid in Sn-2, which has higher nutritional value.
引文
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[20]唐传授,徐建祥.脂肪酸营养功能的最新研究[J]·中国油脂,2000,25(6):20-23
[2]王碧芳,邹锋,袁德义,等.海南油茶优良单株果实经济性状综合评价与筛选[J].福建农林大学学报(自然科学版),2016,(2):156-161
[3]郑道君,潘孝忠,张冬明,等.海南油茶资源调查与分析[J].西北林学院学报,2016,(1):130-135,169
[4]叶洲辰,吴友根,戴俊,等.海南岛白花油茶花粉形态结构的扫描电镜观察[J].热带生物学报,2015,(3):310-314
[5]付登强,杨伟波,陈良秋,等.油茶籽油乙醇提取物抗氧化性研究[J].热带农业科学,2013,(6):62-65
[6]董海洲,万本屹,刘传富,等.葡萄籽油超临界二氧化碳萃取最佳工艺参数及其理化特性的研究[J].中国粮油学报,2004,(5):55-58,63
[7]廖书娟,吉当玲,童华荣.茶油脂肪酸组成及其营养保健功能[J].粮食与油脂,2005,(6):7-9
[8]黄良炳.油中之王——海南山茶油[J].中国土特产,1996,(3):23
[9] SHAO P, SUN PL, YING YJ. Response surface optimi-zation of wheat germ oil yield by supercritical carbon dioxide extraction[J]. Food and Bioproducts Processing,2008,86:227-231
[10]袁向星,杜京霖,宁晖,等.油茶籽油中苯并(a)芘的产生[J].中国油脂,2012,(2):58-61
[11] DUTTA K,GHOSH D,NAZMI A,et al. A Common Carcinogen Benzo[a]pyrene Causes Neuronal Death in Mouse via Microglial Activation[J]. Plo Sone,2010,5(4):e9984
[12]陈岚,满瑞林.超临界萃取技术及其应用研究[J].现代食品科技,2006,(1):199-202
[13]杨春芳,覃小丽,王永华,等.不同部位猪脂的总脂肪酸组成及Sn-2位脂肪酸成分分析[J].中国油脂,2011,(01):73-76
[14]周文化,郑仕宏,蒋爱民.植物油脂脂肪酸组成及位置分布研究进展[J].粮食与油脂,2011,(03):4-6
[15]乔国平,王兴国.功能性油脂-结构脂质[J].粮食与油脂,2002,(9):33-36
[16]徐同成,王文亮,程安玮,等.甘油三酯中脂肪酸分布位置检测方法研究进展[J].粮油加工,2010,(11):35-38
[17]李兴峰,张志国.固相萃取分离甘油三酯水解产物中的单甘酯[J].食品科技,2013,(7):60-62
[18]李存法,何金环.固相萃取技术及其应用[J].天中学刊,2005,(05):13-16
[19] LIN Lei, JING Li, DENG Zeyuan. Predictable Effects of Dietary Lipid Sources on the Fatty Acids Compositions of Four 1 Year-Old Wild Freshwater Fish from Poyang Lake[J]. Journal of Agricultural&Food Chemistry,2013,61(1):210-218
[20]唐传授,徐建祥.脂肪酸营养功能的最新研究[J]·中国油脂,2000,25(6):20-23