急腹症Ⅲ号对呼吸机相关性肺炎大鼠小肠形态的影响
|
摘要
目的观察急腹症Ⅲ号对呼吸机相关性肺炎模型大鼠小肠形态的影响,并探讨其作用机制。方法雄性SD大鼠100只,随机分为五组:急腹症Ⅲ号组(VAP模型,急腹症Ⅲ号灌胃)、谷氨酰胺组(VAP模型,谷氨酰胺颗粒灌胃)、急腹症Ⅲ号联合谷氨酰胺组(VAP模型,急腹症Ⅲ号及谷氨酰胺颗粒灌胃)、安慰剂组(VAP模型,生理盐水灌胃)、空白对照组(生理盐水灌胃)。术后第5天收集尿液,采用L/M(乳果糖/甘露醇法)评价肠黏膜通透性,术后第6天处死大鼠取小肠进行病理检测。结果安慰剂组L/M较空白对照组明显增大(P<0.01),其余三个干预组与空白对照组相似。安慰剂组小肠绒毛高度、黏膜厚度及全层厚度均低于空白对照组(P<0.01)。三个干预组小肠绒毛高度与空白对照组相似(P>0.05),且均高于安慰剂组(P<0.01),联合组绒毛高度高于急腹症Ⅲ号组及谷氨酰胺组(P<0.01),但急腹症Ⅲ号组与谷氨酰胺组之间差异无统计学意义(P>0.05)。空白组黏膜厚度与急腹症Ⅲ号组及联合组相似(P>0.05),但高于谷氨酰胺组(P<0.05),三个干预组均高于安慰剂组(P<0.01),但三组之间无差异(P>0.05)。联合组全层厚度高于安慰剂组(P<0.01),其余组之间无统计学差异(P>0.05)。结论急腹症Ⅲ号可减少呼吸机相关性肺炎大鼠模型肠黏膜通透性增加,减少小肠黏膜结构的破坏,保护肠黏膜结构。
Objective To observe the effect of acute abdomen No.Ⅲ on small intestine morphology in rats with ventilator-associated pneumonia and to explore its mechanism. Methods A total of 100 male SD rats were randomly divided into 5 groups. Acute abdomen No. Ⅲ group: VAP model, acute abdomen No. Ⅲ gavage; glutamine group: VAP model,glutamine granules gavage; acute abdomen No.Ⅲ combined with glutamine group: VAP model, acute abdomen No. Ⅲand glutamine granules gavage; placebo group: VAP model, normal saline gavage; blank control group: normal saline gavage. Urine was collected on the 5 th day after surgery. L/M(lactulose/mannitol method) was used to evaluate the intestinal mucosal permeability. The rats were sacrificed on the 6 th day after surgery to collect the small intestine for pathological examination. Results L/M in placebo group was significantly higher than that in blank control group(P<0.01),and L/M in the other three intervention groups were similar to that in the blank control group. The small intestine villi height, mucosal thickness and full thickness in the placebo group were all lower than those in the blank control group(P<0.01). The small intestine villi height in the three intervention groups was similar to that in the blank control group(P>0.05), and were all higher than that in the placebo group(P<0.01). The villus height in the combined group was higher than that in the acute abdomen No.Ⅲ group and glutamine group(P<0.01). However, there was no difference between acute abdomen No.Ⅲ group and glutamine group(P>0.05). The mucosal thickness in the blank group was similar to that in the acute abdomen No. Ⅲ group and the combined group(P >0.05), but higher than glutamine group(P <0.05). The three intervention groups were higher than placebo group(P<0.01). However, there was no difference between the three groups(P>0.05). The total thickness in the combined group was higher than that in the placebo group(P<0.01), there was no statistically significant difference between the other groups(P>0.05). Conclusion Acute abdomen No.Ⅲ can reduce the increase of intestinal mucosal permeability in rats model with ventilator-associated pneumonia, reduce the destruction of intestinal mucosal structure and protect the structure of intestinal mucosa.
Objective To observe the effect of acute abdomen No.Ⅲ on small intestine morphology in rats with ventilator-associated pneumonia and to explore its mechanism. Methods A total of 100 male SD rats were randomly divided into 5 groups. Acute abdomen No. Ⅲ group: VAP model, acute abdomen No. Ⅲ gavage; glutamine group: VAP model,glutamine granules gavage; acute abdomen No.Ⅲ combined with glutamine group: VAP model, acute abdomen No. Ⅲand glutamine granules gavage; placebo group: VAP model, normal saline gavage; blank control group: normal saline gavage. Urine was collected on the 5 th day after surgery. L/M(lactulose/mannitol method) was used to evaluate the intestinal mucosal permeability. The rats were sacrificed on the 6 th day after surgery to collect the small intestine for pathological examination. Results L/M in placebo group was significantly higher than that in blank control group(P<0.01),and L/M in the other three intervention groups were similar to that in the blank control group. The small intestine villi height, mucosal thickness and full thickness in the placebo group were all lower than those in the blank control group(P<0.01). The small intestine villi height in the three intervention groups was similar to that in the blank control group(P>0.05), and were all higher than that in the placebo group(P<0.01). The villus height in the combined group was higher than that in the acute abdomen No.Ⅲ group and glutamine group(P<0.01). However, there was no difference between acute abdomen No.Ⅲ group and glutamine group(P>0.05). The mucosal thickness in the blank group was similar to that in the acute abdomen No. Ⅲ group and the combined group(P >0.05), but higher than glutamine group(P <0.05). The three intervention groups were higher than placebo group(P<0.01). However, there was no difference between the three groups(P>0.05). The total thickness in the combined group was higher than that in the placebo group(P<0.01), there was no statistically significant difference between the other groups(P>0.05). Conclusion Acute abdomen No.Ⅲ can reduce the increase of intestinal mucosal permeability in rats model with ventilator-associated pneumonia, reduce the destruction of intestinal mucosal structure and protect the structure of intestinal mucosa.
引文
[1]黎介寿.加强对肠屏障功能障碍的研究[J].中华医学杂志,1999,79(8):581.
[2]Van der Hulst RR,Von Meyenfeldt MF,Van Kreel BK,et al.Gut permeability intestinal morphology and nutritional deplerion[J].Nutrition,1998,14(1):1.
[3]武庆平,姚尚龙,袁世萤.呼吸机相关性肺炎动物模型的建立[J].中国危重病急救医学,2005,17(8):501-502.
[4]Liboni KC,Nan Li,Scumpia PO,et a1.Glutamine modulates LPS induced 1L-8 production through Ik B/NF-k B in human fetal and adult hatestinal epithelimn[J].Nutr,2005,135(2):245-251.
[5]Le Voyer T,Cioffi WG,Pratt L,et al.Alterations in imestinal permeability after thermal injury[J].Arch Surg,1992,(127):26-30.
[6]Berg RD,Garlinton AW.Translocation of certain endogenous bacteria from the gastrointestinal tract to the mesenteric lymph nodes and other organs in a gntobiotic mouse modle[J].Infect lmmun,1979,(23):403.
[7]Berg RD.Bacterial translocation from the gastrointestinal tract[J].Adv Exp Med Biol,1999,473:11.
[8]刘坚,郝东宁.丙氨酰谷氨酰胺对重症颅脑损伤患者肠黏膜通透性的影响[J].西南国防医药,2016,26(9):970-972.
[9]徐坡,孙腾,朱洪.中西医结合治疗呼吸机相关性肺炎临床观察[J].山西中医,2016,32(8):19-21.
[10]穆庆平,刘毅,徐玉珩,等.大黄对全身炎症反应综合征患者肠屏障功能保护作用临床研究[J].新中医,2015,47(8):71-72.
[11]乔林.大黄对烫伤后大鼠肠黏膜上皮细胞线粒体功能障碍的影响[J].中华急诊医学杂志,2002,11:16-18.
[12]李军,陈海平,赵金锋,等.中药合剂AA-3对SAP大鼠胰腺结构和功能保护作用的研究[J].中国中医药科技,2008,15(6):410-412.
[13]Moise Coeffier,Pierre Dechelotte.The role of glutamine in Intensive Care Unit patients:Mectmnism of action and clinical outcome[J].Nutrition Rev Jews,2005,63(2):65-69.
[14]刘敏,张长城,顿耀艳.谷氨酰胺对肠道黏膜屏障功能保护作用的研究进展[J].广东医学,2016,37(22):3463-3465.
[15]Smith RJ.Gluttamine memholism and its physiologic importance[J].Parenter Enteral Nur,1999,14(4):40.
[16]Li Y,Li J,Jiang J,et al.Glycyglutmine-supplemented long-term total small bowel autotransplantation in the pig[J].Transpl Int,2003,16(12):866.
[17]张远明,魏民,张鹏,等.谷氨酰胺对重症胃肠功能障碍患者肠功能的保护作用[J].江苏医药,2015,41(21):2594-2595.
[18]王松志,吴乐军,吴应虬,等.谷氨酰胺强化肠外营养对全身炎症反应综合征患者影响的临床研究[J].中国临床药理学杂志,2015,31(8):618-621.
[19]马春燕,王爱丽,王爱娜,等.谷氨酰胺对大鼠肠缺血再灌注损伤后ZO-1的作用及其机制研究[J].中华临床医师杂志,2015,9(9):1644-1648.
[20]徐鑫,孟柠,俞毅君,等.大黄与甘草合用对肠功能衰竭大鼠炎性因子的影响研究[J].中国全科医学,2016,19(15):1824-1827.
[2]Van der Hulst RR,Von Meyenfeldt MF,Van Kreel BK,et al.Gut permeability intestinal morphology and nutritional deplerion[J].Nutrition,1998,14(1):1.
[3]武庆平,姚尚龙,袁世萤.呼吸机相关性肺炎动物模型的建立[J].中国危重病急救医学,2005,17(8):501-502.
[4]Liboni KC,Nan Li,Scumpia PO,et a1.Glutamine modulates LPS induced 1L-8 production through Ik B/NF-k B in human fetal and adult hatestinal epithelimn[J].Nutr,2005,135(2):245-251.
[5]Le Voyer T,Cioffi WG,Pratt L,et al.Alterations in imestinal permeability after thermal injury[J].Arch Surg,1992,(127):26-30.
[6]Berg RD,Garlinton AW.Translocation of certain endogenous bacteria from the gastrointestinal tract to the mesenteric lymph nodes and other organs in a gntobiotic mouse modle[J].Infect lmmun,1979,(23):403.
[7]Berg RD.Bacterial translocation from the gastrointestinal tract[J].Adv Exp Med Biol,1999,473:11.
[8]刘坚,郝东宁.丙氨酰谷氨酰胺对重症颅脑损伤患者肠黏膜通透性的影响[J].西南国防医药,2016,26(9):970-972.
[9]徐坡,孙腾,朱洪.中西医结合治疗呼吸机相关性肺炎临床观察[J].山西中医,2016,32(8):19-21.
[10]穆庆平,刘毅,徐玉珩,等.大黄对全身炎症反应综合征患者肠屏障功能保护作用临床研究[J].新中医,2015,47(8):71-72.
[11]乔林.大黄对烫伤后大鼠肠黏膜上皮细胞线粒体功能障碍的影响[J].中华急诊医学杂志,2002,11:16-18.
[12]李军,陈海平,赵金锋,等.中药合剂AA-3对SAP大鼠胰腺结构和功能保护作用的研究[J].中国中医药科技,2008,15(6):410-412.
[13]Moise Coeffier,Pierre Dechelotte.The role of glutamine in Intensive Care Unit patients:Mectmnism of action and clinical outcome[J].Nutrition Rev Jews,2005,63(2):65-69.
[14]刘敏,张长城,顿耀艳.谷氨酰胺对肠道黏膜屏障功能保护作用的研究进展[J].广东医学,2016,37(22):3463-3465.
[15]Smith RJ.Gluttamine memholism and its physiologic importance[J].Parenter Enteral Nur,1999,14(4):40.
[16]Li Y,Li J,Jiang J,et al.Glycyglutmine-supplemented long-term total small bowel autotransplantation in the pig[J].Transpl Int,2003,16(12):866.
[17]张远明,魏民,张鹏,等.谷氨酰胺对重症胃肠功能障碍患者肠功能的保护作用[J].江苏医药,2015,41(21):2594-2595.
[18]王松志,吴乐军,吴应虬,等.谷氨酰胺强化肠外营养对全身炎症反应综合征患者影响的临床研究[J].中国临床药理学杂志,2015,31(8):618-621.
[19]马春燕,王爱丽,王爱娜,等.谷氨酰胺对大鼠肠缺血再灌注损伤后ZO-1的作用及其机制研究[J].中华临床医师杂志,2015,9(9):1644-1648.
[20]徐鑫,孟柠,俞毅君,等.大黄与甘草合用对肠功能衰竭大鼠炎性因子的影响研究[J].中国全科医学,2016,19(15):1824-1827.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |