基于指纹图谱与聚类分析评价不同产地关升麻质量
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摘要
目的:建立关升麻药材指纹图谱方法,评价不同产地关升麻药材质量。方法:色谱柱为GL Sciences Inertsustain AQ-C18(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸水溶液,柱温为30℃,流速为1.0 m L·min-1,检测波长为317 nm。采用相似度评价、聚类分析、主成分分析方法,对15批样品进行了比较与分析。结果:15批关升麻样品中确定了10个共有峰,相似度在0.80以上,并分为2类。结论:该方法简便、可靠,可以用于不同产地关升麻的质量控制与评价。3个产区中以辽宁省产的关升麻质量最好,同一产区中不同产地间关升麻质量差异较大。
Objective: To establish a method of HPLC fingerprinting and to analyze the quality of Cimicifuga heracleifolia Kom. in different areas. Methods: The column was a GL Sciences Inertsustain AQ-C18 column(250 mm ×4. 6 mm,5 μm). The mobile phase was acetonitrile-0. 1% phosphoric acid solution. The column temperature was 30 ℃,the flow rate was 1. 0 m L·min-1 and the detection wavelength was 317 nm. Ten peaks were extracted as the common peaks of15 batches of samples. The similarity evaluation,cluster analysis and principal component analysis were used to compare and analyze 15 batches of samples. Results: Ten peaks were extracted as the common peaks of 15 batches of samples. the similarity was 0. 80 or more. 15 batches of samples were divided into two categories,and the guality in Liaoning producing areas were better. Conclusion: The method is simple and reliable, and can be used for quality control and evaluation of Cimicifuga heracleifolia Kom. in different areas. The quality of Cimicifuga heracleifolia Kom. in Liaoning producing areas are best and from different places in the same producing areas are different.
Objective: To establish a method of HPLC fingerprinting and to analyze the quality of Cimicifuga heracleifolia Kom. in different areas. Methods: The column was a GL Sciences Inertsustain AQ-C18 column(250 mm ×4. 6 mm,5 μm). The mobile phase was acetonitrile-0. 1% phosphoric acid solution. The column temperature was 30 ℃,the flow rate was 1. 0 m L·min-1 and the detection wavelength was 317 nm. Ten peaks were extracted as the common peaks of15 batches of samples. The similarity evaluation,cluster analysis and principal component analysis were used to compare and analyze 15 batches of samples. Results: Ten peaks were extracted as the common peaks of 15 batches of samples. the similarity was 0. 80 or more. 15 batches of samples were divided into two categories,and the guality in Liaoning producing areas were better. Conclusion: The method is simple and reliable, and can be used for quality control and evaluation of Cimicifuga heracleifolia Kom. in different areas. The quality of Cimicifuga heracleifolia Kom. in Liaoning producing areas are best and from different places in the same producing areas are different.
引文
[1]国家药典委员会编.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:73-74.
[2]南京中医药大学.中药大辞典:上册[M].2版.上海:上海科学技术出版社,2006:621.
[3]林玉萍,邱明华,李忠荣.升麻属植物的化学成分与生物活性研究[J].天然产物研究与开发,2002,14(6):58.
[4]Akiko K,Makio S,Daisuke T,et al.Studies on the constituents of cimicifuga speciesⅩⅩⅧ.Four new cyloart-7-enol glycosides from the underground parts of Cimicifuga simplex Wormsk[J].Chem Pharm Bull,2001,49(4):437.
[5]曾晓辉,丁斐,秦昆明,等.三个不同品种升麻抗炎作用及药效物质基础研究[J].江苏中医药,2017,49(5):75.
[6]肖浩,严小红,江英桥.近红外光谱快速分析广升麻药材[J].中药新药与临床药理,2013,24(5):506-509.
[7]沈保家,秦昆明,张星海,等.基于HPLC指纹图谱的不同产地升麻质量评价研究[J].中国中药杂志,2013,38(13):2155-2158.
[2]南京中医药大学.中药大辞典:上册[M].2版.上海:上海科学技术出版社,2006:621.
[3]林玉萍,邱明华,李忠荣.升麻属植物的化学成分与生物活性研究[J].天然产物研究与开发,2002,14(6):58.
[4]Akiko K,Makio S,Daisuke T,et al.Studies on the constituents of cimicifuga speciesⅩⅩⅧ.Four new cyloart-7-enol glycosides from the underground parts of Cimicifuga simplex Wormsk[J].Chem Pharm Bull,2001,49(4):437.
[5]曾晓辉,丁斐,秦昆明,等.三个不同品种升麻抗炎作用及药效物质基础研究[J].江苏中医药,2017,49(5):75.
[6]肖浩,严小红,江英桥.近红外光谱快速分析广升麻药材[J].中药新药与临床药理,2013,24(5):506-509.
[7]沈保家,秦昆明,张星海,等.基于HPLC指纹图谱的不同产地升麻质量评价研究[J].中国中药杂志,2013,38(13):2155-2158.