白鲜皮饮片-标准汤剂-配方颗粒的HPLC指纹图谱相关性分析
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摘要
目的:建立白鲜皮饮片-标准汤剂-配方颗粒的HPLC指纹图谱,对其质量相关性进行评价。方法:采用HPLC,Inert Sustain C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-水为流动相进行梯度洗脱,检测波长228 nm;流速1.0 m L·min-1,柱温30℃。以对照指纹图谱对白鲜皮饮片-标准汤剂-配方颗粒进行相关性评价。结果:白鲜皮15批饮片、标准汤剂、配方颗粒指纹图谱呈现了10个共有峰,且具有良好的相关性。此外,从10个共有峰中指认出白鲜碱、黄柏酮、梣酮3个化学成分。结论:建立了白鲜皮饮片、标准汤剂、配方颗粒的指纹图谱,全面反映了三者的多组分情况,为白鲜皮配方颗粒质量标准的建立及其全程质量控制提供了参考。
Objective: To establish an HPLC fingerprint spectrum of decoction pieces-standard decoctiondispensing granules of Dictamni Cortex and evaluate the correlation among them. Method: Gradient elution was performed with mobile phase of acetonitrile-water, detection wavelength was set at 228 nm, flow rate was1. 0 m L·min-1,and column temperature was 30 ℃. The correlation of decoction pieces-standard decoctiondispensing granules of Dictamni Cortex was evaluated through the control fingerprint spectrum. Result: There were10 common peaks in all fingerprint spectra of 15 batches of decoction pieces-standard decoction-dispensing granules of Dictamni Cortex with a good correlation. Besides,in the 10 common peaks,three of them were dictamnine,obacunone and fraxinellone. Conclusion: The study establishes an HPLC fingerprint spectrum,and it reflects the whole picture of multi-component of decoction pieces-standard decoction-dispensing granules of Dictamni Cortex. It can provide the reference for establishing quality standard and quality control of dispensing granules of Dictamni Cortex.
Objective: To establish an HPLC fingerprint spectrum of decoction pieces-standard decoctiondispensing granules of Dictamni Cortex and evaluate the correlation among them. Method: Gradient elution was performed with mobile phase of acetonitrile-water, detection wavelength was set at 228 nm, flow rate was1. 0 m L·min-1,and column temperature was 30 ℃. The correlation of decoction pieces-standard decoctiondispensing granules of Dictamni Cortex was evaluated through the control fingerprint spectrum. Result: There were10 common peaks in all fingerprint spectra of 15 batches of decoction pieces-standard decoction-dispensing granules of Dictamni Cortex with a good correlation. Besides,in the 10 common peaks,three of them were dictamnine,obacunone and fraxinellone. Conclusion: The study establishes an HPLC fingerprint spectrum,and it reflects the whole picture of multi-component of decoction pieces-standard decoction-dispensing granules of Dictamni Cortex. It can provide the reference for establishing quality standard and quality control of dispensing granules of Dictamni Cortex.
引文
[1]孙源源,施萍.借助中药配方颗粒推进中药国际化的对策研究[J].中草药,2013,44(8):929-934.
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[4]张玲,李云涛,王丽娜,等.HPLC法测定白鲜皮中白鲜碱的含量[J].中国实验方剂学杂志,2009,15(9):8-9.
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[6]李艳园,彭如习,陈桦,等.HPLC法测定白鲜皮中白鲜碱的含量[J].中药材,2016,29(8):802-803.
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[8]曹娟,王福刚,刘克,等.白鲜皮药材HPLC指纹图谱研究[J].中成药,2011,33(9):1469-1472.
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[10]高峰.HPLC测定白鲜皮配方颗粒中白鲜碱含量[J].黑龙江医药,2015,28(6):1181-1183.
[11]李翠玲,何文斌,高卫东.白鲜皮配方颗粒的质量控制研究[J].中国现代中药,2015,17(7):716-717,725.
[12]王蓓,张云天,李松.白鲜皮配方颗粒高效液相色谱特征图谱研究[J].海峡药学,2015,27(2):47-50.
[13]王爽,王智,侯立强.免煎中药配方颗粒在临床应用中的优势与不足[J].中国现代药物应用,2013,7(14):142-143.
[14]崔景朝,赵自明.中药配方颗粒研究进展(Ⅰ)——文献综合分析[J].中国实验方剂学杂志,2011,17(3):235-238.
[15]吴蓓丽,谢晓梅,况作品,等.宣木瓜药材-饮片-提取物-配方颗粒的质量相关性分析[J].中国实验方剂学杂志,2016,22(5):9-12.
[16]林伟雄,乐智勇,车海燕,等.吴茱萸汤颗粒剂、配方颗粒剂及传统汤剂中指标成分的含量比较[J].中国实验方剂学杂志,2017,23(13):30-34.
[17]陶金华,狄留庆,文红梅,等.中药指纹图谱谱效相关性研究思路探讨[J].中国中药杂志,2009,34(18):2410-2413.
[18]朱丹妮,陈婷,余亚云.高效液相色谱法测定白鲜皮中梣酮含量[J].中国药科大学学报,1998,29(4):319-320.
[19]时东方,郑梅竹,赵立春,等.白鲜皮中白鲜碱的分离及抗炎活性[J].中国实验方剂学杂志,2012,18(14):128-131.
[2]徐珞珊,徐国钧,金蓉鸾.中国药材学[M].北京:中国医药科技出版社,1996:801-802.
[3]国家药典委员会.中华人民共和国药典.一部[M].北京:中国医药科技出版社,2015:110-111.
[4]张玲,李云涛,王丽娜,等.HPLC法测定白鲜皮中白鲜碱的含量[J].中国实验方剂学杂志,2009,15(9):8-9.
[5]白媛媛,唐文照,王晓静.白鲜皮化学成分研究[J].中药材,2014,37(2):263-265.
[6]李艳园,彭如习,陈桦,等.HPLC法测定白鲜皮中白鲜碱的含量[J].中药材,2016,29(8):802-803.
[7]李倩,贾凌云,孙启时.HPLC法同时测定白鲜皮中γ-崖椒碱和白鲜碱的含量[J].沈阳药科大学学报,2010,27(7):570-573.
[8]曹娟,王福刚,刘克,等.白鲜皮药材HPLC指纹图谱研究[J].中成药,2011,33(9):1469-1472.
[9]杜程芳,屠鹏飞.白鲜皮药材指纹图谱研究[J].中国天然药物,2003,1(2):94-98.
[10]高峰.HPLC测定白鲜皮配方颗粒中白鲜碱含量[J].黑龙江医药,2015,28(6):1181-1183.
[11]李翠玲,何文斌,高卫东.白鲜皮配方颗粒的质量控制研究[J].中国现代中药,2015,17(7):716-717,725.
[12]王蓓,张云天,李松.白鲜皮配方颗粒高效液相色谱特征图谱研究[J].海峡药学,2015,27(2):47-50.
[13]王爽,王智,侯立强.免煎中药配方颗粒在临床应用中的优势与不足[J].中国现代药物应用,2013,7(14):142-143.
[14]崔景朝,赵自明.中药配方颗粒研究进展(Ⅰ)——文献综合分析[J].中国实验方剂学杂志,2011,17(3):235-238.
[15]吴蓓丽,谢晓梅,况作品,等.宣木瓜药材-饮片-提取物-配方颗粒的质量相关性分析[J].中国实验方剂学杂志,2016,22(5):9-12.
[16]林伟雄,乐智勇,车海燕,等.吴茱萸汤颗粒剂、配方颗粒剂及传统汤剂中指标成分的含量比较[J].中国实验方剂学杂志,2017,23(13):30-34.
[17]陶金华,狄留庆,文红梅,等.中药指纹图谱谱效相关性研究思路探讨[J].中国中药杂志,2009,34(18):2410-2413.
[18]朱丹妮,陈婷,余亚云.高效液相色谱法测定白鲜皮中梣酮含量[J].中国药科大学学报,1998,29(4):319-320.
[19]时东方,郑梅竹,赵立春,等.白鲜皮中白鲜碱的分离及抗炎活性[J].中国实验方剂学杂志,2012,18(14):128-131.