谷氨酰胺对脓毒症大鼠急性肠黏膜损伤的保护作用
|
摘要
目的:探讨谷氨酰胺对脓毒症大鼠急性肠黏膜损伤的保护作用。方法:将SD大鼠随机分为3组,即正常组(A组)、模型组(B组)及谷氨酰胺组(C组),模型组及谷氨酰胺组大鼠腹腔注射0.45 mg/m L的脂多糖(LPS)溶液,1 m L/100g,即4.5 mg/kg,10 min内分3次注射完毕进行造模。造模12 h后模型组给予25.2%(80 kcal)百普素溶液灌胃,5次/d,每次4 m L;谷氨酰胺组在上述造模12 h后除灌注百普素溶液外,另灌胃谷氨酰胺溶液3.75g/(kg·d)。模型组随机分为喂养36 h组(B1组)和喂养72 h组(B2组)。比较各组组织病理及肠组织谷氨酰胺水平。结果:(1)模型组肠组织谷氨酰胺浓度、绒毛长度及黏膜层厚度均低于正常组(均P<0.05);模型组肠黏膜Chiu氏评分与正常组比较差异有统计学意义(P<0.05)。(2)谷氨酰胺组肠组织谷氨酰胺浓度较模型组升高(P<0.05);谷氨酰胺组肠黏膜Chiu氏评分、绒毛长度及黏膜层厚度较模型组有不同程度改善(均P<0.05)。结论:脓毒症大鼠肠组织谷氨酰胺浓度下降,存在肠黏膜损伤,给予谷氨酰胺治疗能改善脓毒症大鼠的急性肠黏膜损伤。
Objective: To investigate the effect of glutamine on septic acute intestinal mucosa injury in a rat model.Methods: SD rats were divided into three groups: normal group( A),model group( B),and glutamine group( C). The group B was divided into a 36-h subgroup( B1) and a 72 h feeding subgroup( B2). The concentrations of intestinal glutamine and histopathological changes were measured. Results: Intestinal glutamine levels in subgroups B1 and B2( 1. 023 ±0. 039 and 1. 651 ± 0. 036,respectively) were lower than in group A( 2. 924 ± 0. 135,P < 0. 05). The lengths of the villus and thicknesses of the mucosal layer in subgroups B1 and B2,and group C were significantly different from those in group A.The ultrastructural changes of intestines in group B were obvious,while those in group C were not. Intestinal glutamine levels in subgroups B1 and B2( 1. 023 ± 0. 039 and 1. 651 ± 0. 036,respectively) were lower than in group C( 2. 320 ± 0. 157,P < 0. 05). The damage of mucosa was lessened in group C. Conclusions: Treatment with glutamine can decrease intestinal mucosal injury in septic rats.
Objective: To investigate the effect of glutamine on septic acute intestinal mucosa injury in a rat model.Methods: SD rats were divided into three groups: normal group( A),model group( B),and glutamine group( C). The group B was divided into a 36-h subgroup( B1) and a 72 h feeding subgroup( B2). The concentrations of intestinal glutamine and histopathological changes were measured. Results: Intestinal glutamine levels in subgroups B1 and B2( 1. 023 ±0. 039 and 1. 651 ± 0. 036,respectively) were lower than in group A( 2. 924 ± 0. 135,P < 0. 05). The lengths of the villus and thicknesses of the mucosal layer in subgroups B1 and B2,and group C were significantly different from those in group A.The ultrastructural changes of intestines in group B were obvious,while those in group C were not. Intestinal glutamine levels in subgroups B1 and B2( 1. 023 ± 0. 039 and 1. 651 ± 0. 036,respectively) were lower than in group C( 2. 320 ± 0. 157,P < 0. 05). The damage of mucosa was lessened in group C. Conclusions: Treatment with glutamine can decrease intestinal mucosal injury in septic rats.
引文
1 Wischmeyer PE.Glutamine:role in gut protection in critical illness[J].Curr Opin Clin Nutr Metab Care,2006,9(5):607-612.
2 Ruiz P,Tryphonopoulos P,Island E,et al.Citrulline evaluation in bowel transplantation[J].Transplant Proc,2010,42(1):54-56.
3 Shen LJ,Guan YY,Wu XP,et al.Serum citrulline as a diagnostic marker of sepsis-induced intestinal dysfunction[J].Clin Res Hepatol Gastroenterol,2015,39(2):230-236.
4 何艳,秦雪梅,鲁卫华,等.脓毒症大鼠肠道瞬时感受器电位香草酸受体1及降钙素基因相关肽的表达及作用[J].中国临床药理学与治疗学,2016,21(1):38-41.
5 Chiu CJ,Mcardle AH,Brown R,et al.Intestinal mucosa lesion in low flow states[J].Arch Surg,1970,101(4):478-483.
6 Moller SE.Quantification of physiological amino acids by gradient ion exchange high-performance liquid chromatography[J].J Chromatogr,1993,613(2):223-230.
7 Rowlands BJ,Soong CV,Gardiner KR,et al.The gastrointestinal tract as a barrier in sepsis[J].British Medical Bulletin,1999,55(1):196-211.
8 Yoshida S,Kaihra A,Ishibashi N,et al.Glutamine supplementation in cancer patients[J].Nutrition,2001,17(9):766-768.
9 Wilmore DW.The effect of glutamine upplementation in patients following elective surery and accidental injury[J].J Nutr,2001,131(9):2543.
10 翁方中,周瑞祥.重症肺部感染患者应用谷氨酰胺和生长激素免疫营养治疗的临床观察.内科急危重症杂志,2013,19(3):160-167.
11 李宁,赖万强.谷氨酰胺对免疫功能的影响[J].医学综述,2010,16(6):823-826.
12 王辉,范婵娟,张佳莹,等.谷氨酰胺对内毒素血症大鼠肠黏膜屏障的作用[J].中国卫生检验杂志,2013,23(2):355-356.
13 王爱丽,牛琼,刘成霞,等.谷氨酰胺对大鼠肠缺血再灌注损伤的作用[J].中国病理生理杂志,2014,30(9):1703-1707.
14 季艳梅王爱民郭学珍,等.重组人生长激素和谷氨酰胺的强化营养对老年脓毒症患者应激和免疫状态的临床疗效[J].内科急危重症杂志,2013,19(5):288-289.
2 Ruiz P,Tryphonopoulos P,Island E,et al.Citrulline evaluation in bowel transplantation[J].Transplant Proc,2010,42(1):54-56.
3 Shen LJ,Guan YY,Wu XP,et al.Serum citrulline as a diagnostic marker of sepsis-induced intestinal dysfunction[J].Clin Res Hepatol Gastroenterol,2015,39(2):230-236.
4 何艳,秦雪梅,鲁卫华,等.脓毒症大鼠肠道瞬时感受器电位香草酸受体1及降钙素基因相关肽的表达及作用[J].中国临床药理学与治疗学,2016,21(1):38-41.
5 Chiu CJ,Mcardle AH,Brown R,et al.Intestinal mucosa lesion in low flow states[J].Arch Surg,1970,101(4):478-483.
6 Moller SE.Quantification of physiological amino acids by gradient ion exchange high-performance liquid chromatography[J].J Chromatogr,1993,613(2):223-230.
7 Rowlands BJ,Soong CV,Gardiner KR,et al.The gastrointestinal tract as a barrier in sepsis[J].British Medical Bulletin,1999,55(1):196-211.
8 Yoshida S,Kaihra A,Ishibashi N,et al.Glutamine supplementation in cancer patients[J].Nutrition,2001,17(9):766-768.
9 Wilmore DW.The effect of glutamine upplementation in patients following elective surery and accidental injury[J].J Nutr,2001,131(9):2543.
10 翁方中,周瑞祥.重症肺部感染患者应用谷氨酰胺和生长激素免疫营养治疗的临床观察.内科急危重症杂志,2013,19(3):160-167.
11 李宁,赖万强.谷氨酰胺对免疫功能的影响[J].医学综述,2010,16(6):823-826.
12 王辉,范婵娟,张佳莹,等.谷氨酰胺对内毒素血症大鼠肠黏膜屏障的作用[J].中国卫生检验杂志,2013,23(2):355-356.
13 王爱丽,牛琼,刘成霞,等.谷氨酰胺对大鼠肠缺血再灌注损伤的作用[J].中国病理生理杂志,2014,30(9):1703-1707.
14 季艳梅王爱民郭学珍,等.重组人生长激素和谷氨酰胺的强化营养对老年脓毒症患者应激和免疫状态的临床疗效[J].内科急危重症杂志,2013,19(5):288-289.