血清miRNA-143在食管鳞状细胞癌中的表达及意义
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摘要
目的探讨食管鳞状细胞癌患者血清中miRNA-143表达水平的临床意义。方法采用实时荧光定量PCR方法检测150例食管鳞癌患者和80例健康对照患者血清miRNA-143表达水平,分析其与患者临床特征之间的关系,最后采用ROC曲线分析其对食管鳞癌的诊断价值。结果食管鳞癌患者血清miRNA-143表达显著低于对照组(P<0.000 1),且其表达水平与患者的淋巴结转移呈负相关(P<0.01)。ROC曲线分析结果表明,当cut-off值定为0.080时,血清miRNA-143对ESCC诊断敏感度为71.2%,特异度为82.5%,曲线下面积为0.801(95%CI:0.730～0.859)。结论血清miRNA-143在食管鳞癌患者中表达降低,有望作为诊断食管鳞癌的分子标志物。
Objective To investigate the expression and significance of miRNA-143 in serum of patients with esophageal squamous cell carcinoma(ESCC). Methods A total of 150 ESCC cases and 80 healthy individuals were collected. The expression of microRNA-143 was detected by real-time fluorescent RT-PCR. The relationship between its expression and the malignant characteristics of ESCC was explored. And the clinical significance was evaluated by ROC curves. Results The expression of microRNA-143 in the serum of ESCC patients was significantly lower than that in healthy individuals(P<0.0001). The expression of microRNA-143 in ESCC patients was negatively correlated with lymph node metastasis(P<0.01). ROC curves results showed that, when the cut-off value was 0.080, the sensitivity and specificity for ESCC diagnosis were 71.2% and 82.50%, respectively. The area under the ROC curve was 0.801(95% CI: 0.730-0.859). Conclu￣sion The expression level of microRNA-143 in serum of patients with ESCC was decreased, and which should be a new molecular marker for the diagnosis of esophageal squamous cell carcinoma.
Objective To investigate the expression and significance of miRNA-143 in serum of patients with esophageal squamous cell carcinoma(ESCC). Methods A total of 150 ESCC cases and 80 healthy individuals were collected. The expression of microRNA-143 was detected by real-time fluorescent RT-PCR. The relationship between its expression and the malignant characteristics of ESCC was explored. And the clinical significance was evaluated by ROC curves. Results The expression of microRNA-143 in the serum of ESCC patients was significantly lower than that in healthy individuals(P<0.0001). The expression of microRNA-143 in ESCC patients was negatively correlated with lymph node metastasis(P<0.01). ROC curves results showed that, when the cut-off value was 0.080, the sensitivity and specificity for ESCC diagnosis were 71.2% and 82.50%, respectively. The area under the ROC curve was 0.801(95% CI: 0.730-0.859). Conclu￣sion The expression level of microRNA-143 in serum of patients with ESCC was decreased, and which should be a new molecular marker for the diagnosis of esophageal squamous cell carcinoma.
引文
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[2] Pennathur A,Gibson MK,Jobe BA,et al.Oesophageal carcinoma[J].Lancet,2013,381(9864):400-412.
[3] Bartel DP.MicroRNAs:genomics,biogenesis,mechanism,and function[J].Cell,2004,116(2):281-297.
[4] Mitchell PS,Parkin RK,Kroh EM,et al.Circulating microRNAs as stable blood-based markers for cancer detection[J].Proc Natl Acad Sci USA,2008,105(30):10513-10518.
[5] Takagi T,Iio A,Nakagawa Y,et al.Decreased expression of microRNA-143 and -145 in human gastric cancers[J].Oncology,2009,77(1):12-21.
[6] Li JM,Zhao RH,Li ST,et al.Down-regulation of fecal miR-143 and miR-145 as potential markers for colorectal cancer[J].Saudi Med J,2012,33(1):24-29.
[7] Bai JW,Xue HZ,Zhang C.Down-regulation of microRNA-143 is associated with colorectal cancer progression[J].Eur Rev Med Pharmacol Sci,2016,20(22):4682-4687.
[8] Xu B,Liu J,Xiang X,et al.Expression of miRNA-143 in Pancreatic Cancer and Its Clinical Significance[J].Cancer Biother Radiopharm,2018,33(9):373-379.
[9] Zhou M,Chen X,Wu J,et al.MicroRNA-143 regulates cell migration and invasion by targeting GOLM1 in cervical cancer[J].Oncol Lett,2018,16(5):6393-6400.
[10] Zhao Y,Liu X,Lu YX.MicroRNA-143 regulates the proliferation and apoptosis of cervical cancer cells by targeting HIF-1α[J].Eur Rev Med Pharmacol Sci,2017,21(24):5580-5586.
[11] Noguchi S,Yasui Y,Iwasaki J,et al.Replacement treatment with microRNA-143 and -145 induces synergistic inhibition of the growth of human bladder cancer cells by regulating PI3K/Akt and MAPK signaling pathways[J].Cancer Lett,2013,328(2):353-361.
[12] Noguchi S,Mori T,Hoshino Y,et al.MicroRNA-143 functions as a tumor suppressor in human bladder cancer T24 cells[J].Cancer Lett,2011,307(2):211-220.
[13] Xu B,Niu X,Zhang X,et al.miR-143 decreases prostate cancer cells proliferation and migration and enhances their sensitivity to docetaxel through suppression of KRAS[J].Mol Cell Biochem,2011,350(1-2):207-213.
[14] Liu J,Mao Y,Zhang D,et al.MiR-143 inhibits tumor cell proliferation and invasion by targeting STAT3 in esophageal squamous cell carcinoma[J].Cancer Lett,2016,373(1):97-108.
[15] He Z,Yi J,Liu X,et al.MiR-143-3p functions as a tumor suppressor by regulating cell proliferation,invasion and epithelial-mesenchymal transition by targeting QKI-5 in esophageal squamous cell carcinoma[J].Mol Cancer,2016,15(1):51.