应用ROC曲线分析人附睾分泌蛋白4和糖类抗原153在广西早期乳腺癌诊断中的价值
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摘要
目的应用ROC曲线(receiver operating characteristic curve)分析血清人附睾分泌蛋白4(human epididymis protein4,HE4)和糖类抗原153(carbohydrate antigen 153,CA153)在广西早期乳腺癌诊断中的价值。方法用酶联免疫吸附测定(enzyme-linked immunosorbent assay,ELISA)法检测100例Ⅰ~Ⅱ期乳腺癌患者及50例健康对照者血清中HE4和CA153水平,应用ROC曲线(receiver operating characteristic curve)分析HE4和CA153对乳腺癌的诊断价值。结果早期乳腺癌组HE4和CA153水平高于健康对照组,差异有统计学意义(P=0.000),当HE4和CA153的截断值分别为≥99.35 pg/L和≥28.35 U/L时,HE4的AUC为0.897,P=0.000,95%CI:0.848~0.947;CA153的AUC为0.859,P=0.000,95%CI:0.801~0.916,为乳腺癌最佳临床诊断临界点。结论 HE4和CA153水平变化可作为诊断早期乳腺癌重要参考依据。
Objective To explore the diagnostic significance of serum human epididymis protein 4( HE4) and carbohydrate antigen153( CA153) in early stage breast cancer by receiver operating characteristic curve( ROC curve). Methods Serum HE4 and CA153 levels were detected with enzyme-linked immunosorbent( ELISA) in 100 cases Ⅰ-Ⅱ patients with breast cancer surgery and 40 cases with normal healthy controls. We explored the diagnostic significance of serum HE4 and CA153 in breast cancer by ROC curve. Results HE4 and CA153 levels in early stage breast cancer patients were higher than that of the healthy normal group,and the difference was statistically significant( P = 0. 000). It is the best clinical tipping point for the diagnosis of breast cancer when the cutoff value of HE4 and CA153 were ≥99. 35 pg/L and ≥28. 35 U/L,the AUC of HE4 was 0. 897,P = 0. 000,95 % CI: 0. 848-0. 947; the AUC of CA153 was0. 859,P = 0. 000,95 % CI: 0. 801-0. 916. Conclusion The change of HE4 and CA153 levels can be used as important reference for diagnosis of early breast cancer.
Objective To explore the diagnostic significance of serum human epididymis protein 4( HE4) and carbohydrate antigen153( CA153) in early stage breast cancer by receiver operating characteristic curve( ROC curve). Methods Serum HE4 and CA153 levels were detected with enzyme-linked immunosorbent( ELISA) in 100 cases Ⅰ-Ⅱ patients with breast cancer surgery and 40 cases with normal healthy controls. We explored the diagnostic significance of serum HE4 and CA153 in breast cancer by ROC curve. Results HE4 and CA153 levels in early stage breast cancer patients were higher than that of the healthy normal group,and the difference was statistically significant( P = 0. 000). It is the best clinical tipping point for the diagnosis of breast cancer when the cutoff value of HE4 and CA153 were ≥99. 35 pg/L and ≥28. 35 U/L,the AUC of HE4 was 0. 897,P = 0. 000,95 % CI: 0. 848-0. 947; the AUC of CA153 was0. 859,P = 0. 000,95 % CI: 0. 801-0. 916. Conclusion The change of HE4 and CA153 levels can be used as important reference for diagnosis of early breast cancer.
引文
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2 Jemal A,Bray F,Center MM,et al.Global cancer statistics[J].CA Cancer J Clin,2011,61(2):69-90
3 党小军,张华,王欣.人附睾蛋白4(HE4)在乳腺癌诊断中的价值[J].现代检验医学杂志,2015,30(6):84-85
4 刘彩霞,白俊文.血清肿瘤标志物检测对乳腺癌诊断的意义[J].中国医学前沿杂志,2015,7(12):44-46
5 杨泽安.CEA、CA125及CA153在乳腺癌中的检测价值[J].湖南师范大学学报:医学版,2016,13(3):83-85
6 Hertlein L,Stieber P,Kirschenhofer A,et al.Human epididymis protein(HE4)in benign and malignant diseases[J].Clin Chemlab Med,2012,50(12):2181-2188
7 Kirchhoff C,Habben I,Ivell R,et al.A major human epididymisspecific c DNA encodesa protein with sequence homology to extracellular proteinase inhibitors[J].Biol Reprod,1991,45(2):350-357
8 郭变琴,吴立翔.血清人附睾蛋白4、糖类抗原153在监测术后乳腺癌复发转移的探讨[J].检验医学与临床,2015,12(12):1676-1680
9 Kamei M,Yamashita S,Tokuishi K,et al.HE4 expression can be associated with lymph node metastases and diseasefree survival in breast cancer[J].Anticancer Res,2010,30(11):4779-4783
10 刘崇,陈波,姜佳怡,等.血清CA153和HE4检测在乳腺癌的临床意义价值[J].中国微生态学杂志,2015,27(5):565-567
11 Ghadge MS,Naik PP,Tiwari BP,et al.A comparative study of bone scan findings and serum levels of tumor marker CA15-3 in patients with breast carcinoma[J].Indian J Clin Biochem,2012,27(1):97-99
12 Chen Y,Zheng YH,Lin YY,et al.Clinical and prognostic significance of preoperative serum CA15-3,CEA and TPS levels in patients with primary breast cancer[J].Chinese Journal of Oncology,2011,33(11):842-846
13 南永刚,许建林,李楠,等.CA153、CA125联合HER-2检测在乳腺癌各临床分期诊断中的应用[J].现代肿瘤医学,2016,24(19):3070-3073
14 徐龙,张冠中,郑振东,等.血清CEA及CA153连续且显著降低对晚期乳腺癌无影像学进展的判定价值[J].中国肿瘤临床,2016,43(19):865-868
2 Jemal A,Bray F,Center MM,et al.Global cancer statistics[J].CA Cancer J Clin,2011,61(2):69-90
3 党小军,张华,王欣.人附睾蛋白4(HE4)在乳腺癌诊断中的价值[J].现代检验医学杂志,2015,30(6):84-85
4 刘彩霞,白俊文.血清肿瘤标志物检测对乳腺癌诊断的意义[J].中国医学前沿杂志,2015,7(12):44-46
5 杨泽安.CEA、CA125及CA153在乳腺癌中的检测价值[J].湖南师范大学学报:医学版,2016,13(3):83-85
6 Hertlein L,Stieber P,Kirschenhofer A,et al.Human epididymis protein(HE4)in benign and malignant diseases[J].Clin Chemlab Med,2012,50(12):2181-2188
7 Kirchhoff C,Habben I,Ivell R,et al.A major human epididymisspecific c DNA encodesa protein with sequence homology to extracellular proteinase inhibitors[J].Biol Reprod,1991,45(2):350-357
8 郭变琴,吴立翔.血清人附睾蛋白4、糖类抗原153在监测术后乳腺癌复发转移的探讨[J].检验医学与临床,2015,12(12):1676-1680
9 Kamei M,Yamashita S,Tokuishi K,et al.HE4 expression can be associated with lymph node metastases and diseasefree survival in breast cancer[J].Anticancer Res,2010,30(11):4779-4783
10 刘崇,陈波,姜佳怡,等.血清CA153和HE4检测在乳腺癌的临床意义价值[J].中国微生态学杂志,2015,27(5):565-567
11 Ghadge MS,Naik PP,Tiwari BP,et al.A comparative study of bone scan findings and serum levels of tumor marker CA15-3 in patients with breast carcinoma[J].Indian J Clin Biochem,2012,27(1):97-99
12 Chen Y,Zheng YH,Lin YY,et al.Clinical and prognostic significance of preoperative serum CA15-3,CEA and TPS levels in patients with primary breast cancer[J].Chinese Journal of Oncology,2011,33(11):842-846
13 南永刚,许建林,李楠,等.CA153、CA125联合HER-2检测在乳腺癌各临床分期诊断中的应用[J].现代肿瘤医学,2016,24(19):3070-3073
14 徐龙,张冠中,郑振东,等.血清CEA及CA153连续且显著降低对晚期乳腺癌无影像学进展的判定价值[J].中国肿瘤临床,2016,43(19):865-868