一株耐铀镉真菌菌株的筛选及其耐铀镉特性的研究
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摘要
从生长在铀尾矿库区的博落回的根系中,分离出了一株耐铀镉真菌菌株A-2。在固体培养基上,铀和镉对该菌株的最低抑菌浓度(MIC)为160 mg/L和160mg/L,而在液体培养基中,铀和镉对该菌株的MIC为80 mg/L和80 mg/L。经形态学观察和分子鉴定,该菌株属于镰刀菌属,其GenBank登录号为MH978624,可命名为Fusarium sp. A-2。进一步的试验研究结果表明,该菌株在铀镉胁迫下会分泌大量草酸、苹果酸和丁二酸,与铀镉络合,降低了铀镉对菌株的毒性,这可能是该菌株具有高耐铀镉性及能减轻铀镉毒害的机理之一。该菌株在土壤铀镉复合污染治理中具有潜在应用前景。
A uranium-cadmium tolerant fungal strain A-2 was isolated from the roots of Macleaya cordata growing in the uranium tailings repository. In solid medium,the minimum inhibitory concentration(MIC) of uranium and cadmium for this strain is 160 mg/L and160 mg/L. In liquid medium,the MIC of uranium and cadmium for this strain is 80 mg/L and 80 mg/L,respectively. The morphological observation and molecular identification confirmed that the strain belongs to the Fusarium,its GenBank accession number is MH978624,and it can be named Fusarium sp. A-2. In addition,the results from further experiments show that under uranium-cadmium co-stress,the strain can secret large amounts of organic acids including oxalic,malic and succinic acids,which can complex with uranium and cadmium to reduce the toxicity of uranium and cadmium to the strain. This may be the mechanism by which the strain can resist high concentrations of uranium and cadmium and reduce uranium and cadmium toxicities. Fusarium sp. A-2 may have potential application prospects in the remediation of the uranium-cadmium contaminated soils.
A uranium-cadmium tolerant fungal strain A-2 was isolated from the roots of Macleaya cordata growing in the uranium tailings repository. In solid medium,the minimum inhibitory concentration(MIC) of uranium and cadmium for this strain is 160 mg/L and160 mg/L. In liquid medium,the MIC of uranium and cadmium for this strain is 80 mg/L and 80 mg/L,respectively. The morphological observation and molecular identification confirmed that the strain belongs to the Fusarium,its GenBank accession number is MH978624,and it can be named Fusarium sp. A-2. In addition,the results from further experiments show that under uranium-cadmium co-stress,the strain can secret large amounts of organic acids including oxalic,malic and succinic acids,which can complex with uranium and cadmium to reduce the toxicity of uranium and cadmium to the strain. This may be the mechanism by which the strain can resist high concentrations of uranium and cadmium and reduce uranium and cadmium toxicities. Fusarium sp. A-2 may have potential application prospects in the remediation of the uranium-cadmium contaminated soils.
引文
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[15]楚文卉,谢清哲,杨超,等.1株镉耐受真菌C12的鉴定及其镉耐受性研究[J].西北农林科技大学学(自然科学版),2017,45(9):71-80.
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[2]HOREMANS N,VAN HEES M,VAN HOECK A,et al.Uranium and cadmium provoke different oxidative stress responses in Lemna minor L[J].Plant biology,2015,17(1):91-100.
[3]PLANT J A,SIMPSON P R,SMITH B,et al.Uranium ore deposits; products of the radioactive Earth[J]. Reviews in mineralogy and geochemistry,1999,38(1):255-319.
[4]DAS S,DASH R H.1-Microbial Bioremediation:a potential tool for restoration of contaminated areas[J].Microbial biodegradation&bioremediation,2014,2014:1-21.
[5]ABHILASH P C,POWEL J R,SINGH H B,et al.Plantmicrobe interactions:novel applications for exploitation in multipurpose remediation technologies[J]. Trends in biotechnology,2014,30(1):416-420.
[6]WU G,SUN M,LIU P,et al.Enterococcus faecalis strain LZ-11 isolated from Lanzhou reach of the Yellow River is able to resist and absorb Cadmium[J].Journal of applied microbiology,2014,116(5):1172-1180.
[7]MARA-ISABEL DE S,EVA-MARA P,CALLEJO M A,et al.Environmental adaptation factors of two yeasts isolated from the leachate of a uranium mineral heap[J].Fems microbiology Letters,2002,210(2):233-237.
[8]KUMAR R,ACHARYA C,JOSHI R S.Isolation and analyses of uranium tolerant Serratia marcescens strains and their utilization for aerobic uranium U(VI)bioadsorption[J].Journal of microbiology,2011,49(4):568-574.
[9]NEDELKOVA M,MERROUN M L,ROSSBERG A,et al.Microbacterium isolates from the vicinity of a radioactive waste depository and their interactions with uranium[J].Fems microbiology ecology,2010,59(3):694-705.
[10]周赓,杨辉,潘虎,等.一株耐镉链霉菌的筛选、鉴定与基本特性分析[J].环境科学学报,2017,37(6):2076-2084.
[11] TAMANDEGANI P R,ZAFARI D.Evaluation of different Fusarium species-wheat interactions effect on Cd biosorption by wheat seedlings[J].International journal of environmental science&technology,2017(1):1-12.
[12]KHAN A R,WAQAS M,ULLAH I,et al.Culturable endophytic fungal diversity in the cadmium hyperaccumulator Solanum nigrum L. and their role in enhancing phytoremediation[J]. Environmental&experimental botany,2016,135:126-135.
[13]NIE J,LIU Y,ZENG G,et al.Cadmium accumulation and tolerance of Macleaya cordata:a newly potential plant for sustainable phytoremediation in Cd-contaminated soil[J].Environmental science&pollution research,2016,23(10):10189-10199.
[14]陈威,胡南,陈可,等.博落回和竹柳间作修复铀污染土壤的研究[J].原子能科学技术,2018,52(10):1748-1755.
[15]楚文卉,谢清哲,杨超,等.1株镉耐受真菌C12的鉴定及其镉耐受性研究[J].西北农林科技大学学(自然科学版),2017,45(9):71-80.
[16]BAN Y,TANG M,CHEN H,et al.The response of dark septate endophytes(DSE)to heavy metals in pure culture[J].Plos one,2012,7(10):479-68.
[17]冷阳.黄孢原毛平革菌对重金属镉的富集及其交互作用机理研究[D].长沙:湖南大学,2014.
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