头针结合艾灸对缺血缺氧大脑损伤幼鼠基因蛋白NGF、BDNF表达的影响
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摘要
目的:观察头针结合艾灸对大脑缺血缺氧幼鼠基因蛋白神经生长因子(nerve growth factor,NGF)及脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)表达的影响。方法:选取造模成功的120只幼鼠为研究对象,随机分为空白对照组、头针组、艾灸组、头针结合艾灸组,每组各40只。空白对照组不予以任何治疗,头针组给予头针治疗,艾灸组给予艾灸治疗,头针结合艾灸组给予头针结合艾灸治疗。结果:造模后头针结合艾灸组NGF、BDNF水平高于头针组、艾灸组、空白对照组,差异具有统计学意义(P <0. 05);头针组、艾灸组NGF、BDNF水平比较,差异无统计学意义(P>0. 05)。造模后头针结合艾灸组反应时间短于头针组、艾灸组、空白对照组,差异具有统计学意义(P <0. 05);头针组、艾灸组反应时间比较,差异无统计学意义(P> 0. 05)。结论:头针结合艾灸可有效上调幼鼠海马区缺血缺氧脑组织中NGF、BDNF基因蛋白水平的表达。
Objective: To observe the influence of scalp acupuncture in combination with moxibustion on the expression of gene proteins nerve growth factor( NGF) and brain-derived neurotrophic factor( BDNF) in young rats with cerebral ischemia-hypoxia injury. Methods: A total of 120 young rats were randomly divided into the blank control group,the scalp acupuncture group,the moxibustion group,the scalp acupuncture combined with moxibustion group,with 40 rats in each group. The blank control group was not given any treatment,the scalp acupuncture group was given scalp acupuncture treatment,the moxibustion group was given moxibustion treatment,and the scalp acupuncture combined with moxibustion group was given scalp acupuncture combined with moxibustion treatment. Results: After successful modeling,the levels of NGF and BDNF in the scalp acupuncture combined with moxibustion group were higher than those in the scalp acupuncture group,the moxibustion group and blank control group,and the difference was statistically significant( P < 0. 05). There was no significant difference in the levels of NGF and BDNF between the scalp acupuncture group and the moxibustion group( P > 0. 05). After successful modeling,the reaction time of the scalp acupuncture combined with moxibustion group was shorter than that of the scalp acupuncture group,the moxibustion group and blank control group,and the difference was statistically significant( P < 0. 05). And there was no significant difference in reaction time between the scalp acupuncture group and the moxibustion group( P > 0. 05). Conclusion: Scalp acupuncture combined with moxibustion can effectively up-regulate the expression of NGF and BDNF gene protein in hippocampal ischemic and hypoxic brain tissue of young rats.
Objective: To observe the influence of scalp acupuncture in combination with moxibustion on the expression of gene proteins nerve growth factor( NGF) and brain-derived neurotrophic factor( BDNF) in young rats with cerebral ischemia-hypoxia injury. Methods: A total of 120 young rats were randomly divided into the blank control group,the scalp acupuncture group,the moxibustion group,the scalp acupuncture combined with moxibustion group,with 40 rats in each group. The blank control group was not given any treatment,the scalp acupuncture group was given scalp acupuncture treatment,the moxibustion group was given moxibustion treatment,and the scalp acupuncture combined with moxibustion group was given scalp acupuncture combined with moxibustion treatment. Results: After successful modeling,the levels of NGF and BDNF in the scalp acupuncture combined with moxibustion group were higher than those in the scalp acupuncture group,the moxibustion group and blank control group,and the difference was statistically significant( P < 0. 05). There was no significant difference in the levels of NGF and BDNF between the scalp acupuncture group and the moxibustion group( P > 0. 05). After successful modeling,the reaction time of the scalp acupuncture combined with moxibustion group was shorter than that of the scalp acupuncture group,the moxibustion group and blank control group,and the difference was statistically significant( P < 0. 05). And there was no significant difference in reaction time between the scalp acupuncture group and the moxibustion group( P > 0. 05). Conclusion: Scalp acupuncture combined with moxibustion can effectively up-regulate the expression of NGF and BDNF gene protein in hippocampal ischemic and hypoxic brain tissue of young rats.
引文
[1]徐艳,谢文龙,何凤翔,等.躯干控制训练对小儿脑瘫运动功能及日常生活活动能力的影响[J].中华物理医学与康复杂志,2012,34(11):825-827.
[2]张宁霞,刘桂珍,郝晋东,等.热补针法结合康复训练对小儿脑瘫患儿功能影响的随机对照研究[J].针刺研究,2007,32(4):260-263.
[3]周钰,景福权,牛相来,等.针灸结合康复训练治疗小儿脑瘫[J].吉林中医药,2015,35(12):1283-1285.
[4] LIMA J E,TAKEYANAGUI O M,GARCIA L V,et al. Neuronspecificenolase in patients with neurocysticercosis[J]. J Neurol Sci,2004,217(1):31-35.
[5]周钰,牛相来,方丽娜,等.头针结合艾灸对缺血缺氧性脑损伤幼鼠学习记忆能力及脑内BDFN的影响[J].吉林中医药,2015,35(2):181-183.
[6]张刘通,于学平,滕伟.百会透曲鬓穴为主治疗小儿脑瘫疗效观察[J].针灸临床杂志,2011,27(11):41-43.
[7]刘毅梅,惠需,徐晓华,等.血管内皮生长因子介导P13K—e NOS信号通路对脑瘫模型大鼠神经损伤的保护作用[J].中华物理医学与康复杂志,2015,37(1):56-59.
[8]张雨平.缺氧缺血性脑损伤新生大鼠髓鞘损伤与修复的相关研究[D].重庆:第三军医大学,2011.
[9]姚凤莉,肖红池,黄捷婷,等.中西医结合对脑瘫高危儿不同时期干预的预后分析[J].中国中医急症,2008,17(10):1358-1391.
[10]朱南方,李小苹,李小蓉.针灸综合治疗小儿脑瘫近况[J].中国中医急症,2009,18(8):1325-1328.
[11]牛相来,周钰,周伟,等.头针治疗小儿脑瘫临床疗效的Meta分析[J].中国中西医结合急救杂志,2015,22(4):347-349.
[12]章显宝,汪瑛,王震,等.项丛刺针法对缺血性脑卒中后遗症大鼠BDNF、NGF以及神经行为学的影响[J].上海针灸杂志,2014,33(2):181-183.
[13]李育鑫,袁绍纪,朱伟杰,等. PAR-1拮抗剂SCH79797对SAH大鼠早期脑损伤的保护作用及对BDNF﹑NGF表达的影响[J].中华神经医学杂志,2016,15(7):700-703.
[14]韩宁,张青,房位昊.荣络健脑饮对脑缺血再灌注大鼠NGF、BDNF、bFGF表达的影响[J].中国中医急症,2012,21(9):1425-1428.
[15]景福权,牛相来,周钰,等.透灸法结合电针治疗风寒痹阻型神经根型颈椎病的临床观察[J].中国中西医结合急救杂志,2015,22(4):367-369.
[2]张宁霞,刘桂珍,郝晋东,等.热补针法结合康复训练对小儿脑瘫患儿功能影响的随机对照研究[J].针刺研究,2007,32(4):260-263.
[3]周钰,景福权,牛相来,等.针灸结合康复训练治疗小儿脑瘫[J].吉林中医药,2015,35(12):1283-1285.
[4] LIMA J E,TAKEYANAGUI O M,GARCIA L V,et al. Neuronspecificenolase in patients with neurocysticercosis[J]. J Neurol Sci,2004,217(1):31-35.
[5]周钰,牛相来,方丽娜,等.头针结合艾灸对缺血缺氧性脑损伤幼鼠学习记忆能力及脑内BDFN的影响[J].吉林中医药,2015,35(2):181-183.
[6]张刘通,于学平,滕伟.百会透曲鬓穴为主治疗小儿脑瘫疗效观察[J].针灸临床杂志,2011,27(11):41-43.
[7]刘毅梅,惠需,徐晓华,等.血管内皮生长因子介导P13K—e NOS信号通路对脑瘫模型大鼠神经损伤的保护作用[J].中华物理医学与康复杂志,2015,37(1):56-59.
[8]张雨平.缺氧缺血性脑损伤新生大鼠髓鞘损伤与修复的相关研究[D].重庆:第三军医大学,2011.
[9]姚凤莉,肖红池,黄捷婷,等.中西医结合对脑瘫高危儿不同时期干预的预后分析[J].中国中医急症,2008,17(10):1358-1391.
[10]朱南方,李小苹,李小蓉.针灸综合治疗小儿脑瘫近况[J].中国中医急症,2009,18(8):1325-1328.
[11]牛相来,周钰,周伟,等.头针治疗小儿脑瘫临床疗效的Meta分析[J].中国中西医结合急救杂志,2015,22(4):347-349.
[12]章显宝,汪瑛,王震,等.项丛刺针法对缺血性脑卒中后遗症大鼠BDNF、NGF以及神经行为学的影响[J].上海针灸杂志,2014,33(2):181-183.
[13]李育鑫,袁绍纪,朱伟杰,等. PAR-1拮抗剂SCH79797对SAH大鼠早期脑损伤的保护作用及对BDNF﹑NGF表达的影响[J].中华神经医学杂志,2016,15(7):700-703.
[14]韩宁,张青,房位昊.荣络健脑饮对脑缺血再灌注大鼠NGF、BDNF、bFGF表达的影响[J].中国中医急症,2012,21(9):1425-1428.
[15]景福权,牛相来,周钰,等.透灸法结合电针治疗风寒痹阻型神经根型颈椎病的临床观察[J].中国中西医结合急救杂志,2015,22(4):367-369.