基因芯片检测技术在禽类病原检测方面的研究进展
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摘要
基因芯片是继PCR后发展起来的一项快速、特异性好、敏感性强及高通量等优点的自动化基因检测技术,被广泛地应用于药物筛选、基因研究和疾病诊断检测等方面。随着病原不断发生基因重组,变异等现象,禽类病毒不断逃避免疫保护导致了强毒株的出现。传统的芯片检测方法虽然能够实现病毒的检测,然而昂贵的仪器设备和较长的检测时间严重阻碍了芯片技术在实际生产中的应用。一种低成本、时间短、快速有效的新的基因芯片技术的开发和应用是解决当前禽业产业发展的必要手段。基因芯片检测技术方法的改良及应用已成为目前疾病诊断的研究热点。本研究主要综述了基因芯片检测技术在禽类疾病检测方面的发展现状,并对今后该技术的发展方向和重点作了展望。
DNA microarray is a genetic testing automation technology,which has the advantages of being fast,specific,sensitive and high-throughput.It is currently the major means for performing drug analyses,genome research,and also for disease diagnosis.Due to the continuous occurrence of gene recombination and mutation,influent escaping of avian virus from the protection immune system led to the generation of velogen strain virus.Despite of the utilization of traditional chip detection method,the expensive equipment and time-costing process significantly inhibited the application of chip for the detection of avian disease.A cheaper,more efficient and time-saving chip detection method is needed to meet the development of poultry industry.This requirement has highlighted the improvement and application of gene chip detection to be a hot research issue around disease diagnosis currently.In this review,the developing situation of DNA microarray in response to avian disease detection,eventually,the future research direction and emphasis of DNA microarray were looked forward.
DNA microarray is a genetic testing automation technology,which has the advantages of being fast,specific,sensitive and high-throughput.It is currently the major means for performing drug analyses,genome research,and also for disease diagnosis.Due to the continuous occurrence of gene recombination and mutation,influent escaping of avian virus from the protection immune system led to the generation of velogen strain virus.Despite of the utilization of traditional chip detection method,the expensive equipment and time-costing process significantly inhibited the application of chip for the detection of avian disease.A cheaper,more efficient and time-saving chip detection method is needed to meet the development of poultry industry.This requirement has highlighted the improvement and application of gene chip detection to be a hot research issue around disease diagnosis currently.In this review,the developing situation of DNA microarray in response to avian disease detection,eventually,the future research direction and emphasis of DNA microarray were looked forward.
引文
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[26]郭永刚,张冠斌,熊强,等.基于磁珠的可见光检测微阵列信号的新方法[J].分析科学学报,2007,23(1):1-4.
[27]LEBLANC N,GANTELIUS J,SCHWENK J M,et al.Development of a magnetic bead microarray for simultaneous and simple detection of four pestiviruses[J].J Virol Methods,2009,155(1):1-9.
[28]LI S,LIU H,DENG Y,et al.Development of a magnetic nanoparticles microarray for simultaneous and simple detection of foodborne pathogens[J].J Biomed Nanotechnol,2013,9(7):1254-1260.
[29]TANG J,ZHOU L,GAO W,et al.Visual DNA microarrays for simultaneous detection of human immunodeficiency virus type-1 and Treponema pallidum coupled with multiplex asymmetric polymerase chain reaction[J].Diagn Microbiol Infect Dis,2009,65(4):372-378.
[30]ZOU N L,ZANG Y C,MAO H J,et al.A visual highsensitivity microarray for detection of hepatitis B virus DNA and its single nucleotide polymorphisms[J].Chin J Analytical Chem,2012,40(4):569-573.
[31]WANG X,DANG E,GAO J,et al.Development of a gold nanoparticle-based oligonucleotide microarray for simultaneous detection of seven swine viruses[J].JVirol Methods,2013,191(1):9-15.
[32]XUE L,FEI J J,SONG Y,et al.Visual DNA microarray for detection of epidermal growth factor receptor(EGFR)gene mutations[J].Scand J Clin Lab Invest,2014,74(8):693-699.
[33]HE Y,ZHANG X,ZHANG S,et al.Visual detection of single-base mismatches in DNA using hairpin oligonucleotide with double-target DNA binding sequences and gold nanoparticles[J].Biosens Bioelectron,2012,34(1):37-43.
[34]ZHANG Y Q,WANG Y F,JIANG X D.The application of nanoparticles in biochips[J].Recent Pat Biotechnol,2008,2(1):55-59.
[35]QI H J,CHEN S H,ZHANG M L,et al.DNA microarrays for visual detection of human pathogenic microorganisms based on tyramine signal amplification coupled with gold label silver stain[J].Anal Bioanal Chem,2010,398(6):2745-2750.
[36]张春,刘琪琦,陈苏红,等.酪胺信号放大-量子点标记银染增强的基因芯片可视化检测方法的建立[J].生物技术通讯,2012,23(4):558-562.
[2]HAN X,LIN X,LIU B,et al.Simultaneously subtyping of all influenza A viruses using DNA microarrays[J].J Virol Methods,2008,152(1):117-121.
[3]GOPAL S,MANOHARAN P,KATHAPERUMALK,et al.Differential detection of avian oncogenic viruses in poultry layer farms and Turkeys by use of multiplex PCR[J].J Clin Microbiol,2012,50(8):2668-2673.
[4]SCHENA M,SHALON D,DAVIS R W,et al.Quantitative monitoring of gene expression patterns with a complementary DNA microarray[J].Science,1995,270(5235):467-470.
[5]IVSHINA A V,VODEIKO G M,KUZNETSOV VA,et al.Mapping of genomic segments of influenza Bvirus strains by an oligonucleotide microarray method[J].J Clin Microbiol,2004,42(12):5793-5801.
[6]ROTA P A,OBERSTE M S,MONROE S S,et al.Characterization of a novel coronavirus associated with severe acute respiratory syndrome[J].Science,2003,300(5624):1394-1399.
[7]WANG L C,HUANG D,PU C E,et al.Avian oncogenic virus differential diagnosis in chickens using oligonucleotide microarray[J].J Virol Methods,2014,210:45-50.
[8]LI J,CHEN S,EVANS D H.Typing and subtyping influenza virus using DNA microarrays and multiplex reverse transcriptase PCR[J].J Clin Microbiol,2001,39(2):696-704.
[9]KESSLER N,FERRARIS O,PALMER K,et al.Use of the DNA flow-thru chip,a three-dimensional biochip,for typing and subtyping of influenza viruses[J].J Clin Microbiol,2004,42(5):2173-2185.
[10]TOWNSEND M B,DAWSON E D,MEHLMANNM,et al.Experimental evaluation of the FluChip diagnostic microarray for influenza virus surveillance[J].JClin Microbiol,2006,44(8):2863-2871.
[11]SENGUPTA S,ONODERA K,LAI A,et al.Molecular detection and identification of influenza viruses by oligonucleotide microarray hybridization[J].J Clin Microbiol,2003,41(10):4542-4550.
[12]LEE D H,CHUNG I H,ROH M R,et al.A diagnostic microarray for subtyping and pathotyping avian influenza virus[J].Biochip J,2009,3(1):57-64.
[13]TAO Q,WANG X,BAO H,et al.Detection and differentiation of four poultry diseases using asymmetric reverse transcription polymerase chain reaction in combination with oligonucleotide microarrays[J].JVet Diagn Invest,2009,21(5):623-632.
[14]CITARTAN M,TANG T H,TAN S C,et al.Asymmetric PCR for good quality ssDNA generation towards DNA aptamer production[J].Songklanakarin JSci Technol,2012,34(2):125-131.
[15]LEE D H,KIM J H,YUK S S,et al.Rapid hemagglutinin subtyping of novel avian-origin influenza A(H7N9)virus using a diagnostic microarray[J].Biochip J,2014,8(1):55-59.
[16]UYEKI T M,COX N J.Global concerns regarding novel influenza A(H7N9)virus infections[J].New England J Med,2013,368(20):1862-1864.
[17]SULTANKULOVA K T,KOZHABERGENOV NS,STROCHKOV V M,et al.New oligonucleotide microarray for rapid diagnosis of avian viral diseases[J].Virol J,2017,14(1):69.
[18]TATON T A,MIRKIN C A,LETSINGER R L.Scanometric DNA array detection with nanoparticle probes[J].Science,2000,289(5485):1757-1760.
[19]TAO S C,ZHANG X E,LIU H,et al.Multiplex PCRfor simultaneous detection of human carcinoma-related viruses[J].Analytical Lett,2002,35(7):1149-1162.
[20]LI Y.Establishment and application of a visual DNAmicroarray for the detection of food-borne pathogens[J].Anal Sci,2016,32(2):215-218.
[21]MONECKE S,EHRICHT R.Rapid genotyping of methicillin-resistant Staphylococcus aureus(MRSA)isolates using miniaturised oligonucleotide arrays[J].Clin Microbiol Infect,2010,11(10):825-833.
[22]GALL A,HOFFMANN B,HARDER T,et al.Rapid haemagglutinin subtyping and pathotyping of avian influenza viruses by a DNA microarray[J].J Virol Methods,2009,160(1):200-205.
[23]CAO X,WANG Y F,ZHANG C F,et al.Visual DNAmicroarrays for simultaneous detection of Ureaplasma urealyticum and Chlamydia trachomatis coupled with multiplex asymmetrical PCR[J].Biosens Bioelectron,2006,22(3):393-398.
[24]WANG L,LIU B,WANG T,et al.Simultaneous detection and differentiation of Newcastle disease and avian influenza viruses using oligonucleotide microarrays[J].Vet Microbiol,2008,127(3):217-226.
[25]WANG L C,HUANG D,CHENG M C,et al.H5avian influenza virus pathotyping using oligonucleotide microarray[J].J Virol Methods,2015,220:39-42.
[26]郭永刚,张冠斌,熊强,等.基于磁珠的可见光检测微阵列信号的新方法[J].分析科学学报,2007,23(1):1-4.
[27]LEBLANC N,GANTELIUS J,SCHWENK J M,et al.Development of a magnetic bead microarray for simultaneous and simple detection of four pestiviruses[J].J Virol Methods,2009,155(1):1-9.
[28]LI S,LIU H,DENG Y,et al.Development of a magnetic nanoparticles microarray for simultaneous and simple detection of foodborne pathogens[J].J Biomed Nanotechnol,2013,9(7):1254-1260.
[29]TANG J,ZHOU L,GAO W,et al.Visual DNA microarrays for simultaneous detection of human immunodeficiency virus type-1 and Treponema pallidum coupled with multiplex asymmetric polymerase chain reaction[J].Diagn Microbiol Infect Dis,2009,65(4):372-378.
[30]ZOU N L,ZANG Y C,MAO H J,et al.A visual highsensitivity microarray for detection of hepatitis B virus DNA and its single nucleotide polymorphisms[J].Chin J Analytical Chem,2012,40(4):569-573.
[31]WANG X,DANG E,GAO J,et al.Development of a gold nanoparticle-based oligonucleotide microarray for simultaneous detection of seven swine viruses[J].JVirol Methods,2013,191(1):9-15.
[32]XUE L,FEI J J,SONG Y,et al.Visual DNA microarray for detection of epidermal growth factor receptor(EGFR)gene mutations[J].Scand J Clin Lab Invest,2014,74(8):693-699.
[33]HE Y,ZHANG X,ZHANG S,et al.Visual detection of single-base mismatches in DNA using hairpin oligonucleotide with double-target DNA binding sequences and gold nanoparticles[J].Biosens Bioelectron,2012,34(1):37-43.
[34]ZHANG Y Q,WANG Y F,JIANG X D.The application of nanoparticles in biochips[J].Recent Pat Biotechnol,2008,2(1):55-59.
[35]QI H J,CHEN S H,ZHANG M L,et al.DNA microarrays for visual detection of human pathogenic microorganisms based on tyramine signal amplification coupled with gold label silver stain[J].Anal Bioanal Chem,2010,398(6):2745-2750.
[36]张春,刘琪琦,陈苏红,等.酪胺信号放大-量子点标记银染增强的基因芯片可视化检测方法的建立[J].生物技术通讯,2012,23(4):558-562.