左旋龙脑的不同提取工艺对比研究及其HHLSE提取工艺优化
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摘要
目的比较不同方法提取艾纳香中左旋龙脑的提取效率,并对最优方法进行工艺优化。方法本研究比较了水蒸气蒸馏法(传统提取方法)、超声萃取法及高效高压差低温连续式提取法(HHLSE)对左旋龙脑提取效率的影响,并通过正交试验优化HHLSE提取工艺。结果左旋龙脑的HHLSE提取率高达88%,远高于传统提取方法的效率。此外,正交试验确定HHLSE最佳工艺为:提取溶剂75%乙醇,溶剂倍量1:20,提取压差-0.1~35 Mpa。结论优选的HHLSE提取工艺高效、快速、稳定,为左旋龙脑的工业化生产提供新的技术指导。
Objective To compare the extraction methods for L-borneol from Blumea balsamifera(L.) DC., and to optimize the extraction conditions for the optimum extraction method. Methods The effects of steam distillation extraction(traditional extraction method), ultrasonic extraction and the high efficiency, high differential pressure and low temperature successive extraction(HHLSE) on yield of L-borneol were investigated. Optimization of the HHLSE was carried out by the orthogonal experiment. Results It was shown that the yield of the HHLSE was about 88 %, and it was far higher than the efficiency of traditional extraction method. In addition, the optimum extraction process was as follows: the medicinal material was extracted with 20 times of 75 % ethanol, and the extraction pressure was-0.1-35 Mpa. Conclusion The optimum HHLSE process is efficient, fast and stable, which can provide new technical support for industrial production of L-borneol.
Objective To compare the extraction methods for L-borneol from Blumea balsamifera(L.) DC., and to optimize the extraction conditions for the optimum extraction method. Methods The effects of steam distillation extraction(traditional extraction method), ultrasonic extraction and the high efficiency, high differential pressure and low temperature successive extraction(HHLSE) on yield of L-borneol were investigated. Optimization of the HHLSE was carried out by the orthogonal experiment. Results It was shown that the yield of the HHLSE was about 88 %, and it was far higher than the efficiency of traditional extraction method. In addition, the optimum extraction process was as follows: the medicinal material was extracted with 20 times of 75 % ethanol, and the extraction pressure was-0.1-35 Mpa. Conclusion The optimum HHLSE process is efficient, fast and stable, which can provide new technical support for industrial production of L-borneol.
引文
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[12]刘常青,段海霞,宋力飞,等.不同时期艾纳香药材的指纹图谱研究[J].亚太传统医药,2016,12(21):28-31.
[2]Ceacero-Vega A,Ballesteros B,Bejan I,et al.Kinetics and mechanisms of the tropospheric reactions of menthol,borneol,fenchol,camphor,and fenchone with hydroxyl radicals(OH)and chlorine atoms(Cl)[J].J Phys Chem A,2012,116:4097-4107.
[3]国家药典委员会.中华人民共和国药典[S].一部.北京:中国医药科技出版社,2015:88-89.
[4]谭道鹏,严启新,康晖,等.艾纳香化学成分的研究[J].天然产物研究与开发,2012,24:718-721.
[5]Bhatia S P,Letizia C S,Api A M.Fragrance material review on L-borneol[J].Food Chem Toxicol,2008,46(11):81-84.
[6]曾建国.天然冰片的研究进展[J].中国药物经济学,2011,(2):82-89.
[7]姜建昌,曹雷,姚瑜洁,等.冰片的临床应用研究概况[J].现代中西医结合杂志,2013(2):221-223.
[8]王远辉,赵丹丹,王洪新,等.GC测定艾纳香叶中左旋龙脑、异龙脑、樟脑的不同预处理方法比较[J].现代食品科技,2014,(6):250-256.
[9]周立雄,宋力飞,刘乡乡,等.移动式高压差连续提取分离装置及其自动控制系统[J].包装与机械,2014,30(4):89-91.
[10]宋力飞,刘常青,覃婕媛,等.淫羊藿中淫羊藿苷的高效高压差低温连续式提取技术(HHLSE)研究[J].亚太传统医药,2013,9(6):50-52.
[11]刘乡乡,颜东亮,周绍春,等.巴戟天的高效高压差低温连续式提取技术(HHLSE)研究[J].中国医药指南,2013,11(21):458-459.
[12]刘常青,段海霞,宋力飞,等.不同时期艾纳香药材的指纹图谱研究[J].亚太传统医药,2016,12(21):28-31.