维生素D结合蛋白基因遗传变异与海南省老龄慢性鼻窦炎的关联性
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摘要
目的探讨维生素D结合蛋白(VDBP)基因GC遗传变异与海南者老龄慢性鼻窦炎的关联性。方法 37例老龄慢性鼻窦炎患者作为病例组,99例健康老年体检者作为对照组,检测GC基因三个多态性位点rs7041、rs222016、rs4588的基因型,利用统计学在线分析软件SNPStats进行数据分析。结果三个单核苷酸多态性(SNP)位点均可以检测出三种基因型,其中rs7041的三个基因型TT、GT、GG在病例组的发生率为20例(54.1%)、14例(37.8%)、3例(8.1%),在对照组分别为44例(44.4%)、41例(41.4%)、14例(14.2%)。rs222016的三种基因型AA、AG、GG在病例组的发生率分别为14例(37.8%)、17例(46.0%)、6例(16.2%),在对照组分别为34例(34.3%)、39例(39.4%)、26例(26.3%)。rs4588的三种基因型CC、AC、AA在病例组的发生率为17例(46.0%)、18例(48.6%)、2例(5.4%),在对照组分别为59例(59.6%)、33例(33.3%)、7例(7.1%)。三个SNP位点基因型及等位基因在组间的分布频率均无统计学意义(P>0.05)。结论 GC基因三个多态性位点rs7041、rs222016、rs4588的遗传差异可能与海南老龄慢性鼻窦炎无关联。
Objective To explore the correlation between the genetic variation of vitamin D binding protein GC and sinusitis of the elderly patients in Hainan province. Methods 37 cases of elderly chronic sinusitis patients and 99 cases of healthy elderly controls were chosen as the research samples.The genomic DNA was detected by the snapshot technology to distinguish the genotype of three single nucleotide polymorphism loci, rs222016, rs4588 and rs7041 on GC gene. The data were analyzed with online SNPstats and SPSS25.0. Results Three genotypes were founded for all of the three loci. The frequencies of TT, GT and GG genotypes of rs7041 were 20(54.1%), 14(37.8%) and 3(8.1%) in the case group, and 44(44.4%), 41(41.4%) and 14(14.2%) in the control group, respectively. The frequencies of AA, AG and GG of rs222016 in the case group were 14(37.8%), 17(46.0%), 6(16.2%), and 34(34.3%), 39(39.4%), 26(26.3%) in the control group respectively. The frequencies of CC, AC and AA of rs4588 were 17(46.0%), 18(48.6%), 2(5.4%) in the case group, and 59(59.6%), 33(33.3%), 7(7.1%) in the control group. There were no statistical differences between the two groups(P>0.05).Conclusions The genetic differences of three polymorphisms of GC gene, including rs7041, rs222016 and rs4588, might not be associated with the elderly patients with sinusitis in Hainan province.
Objective To explore the correlation between the genetic variation of vitamin D binding protein GC and sinusitis of the elderly patients in Hainan province. Methods 37 cases of elderly chronic sinusitis patients and 99 cases of healthy elderly controls were chosen as the research samples.The genomic DNA was detected by the snapshot technology to distinguish the genotype of three single nucleotide polymorphism loci, rs222016, rs4588 and rs7041 on GC gene. The data were analyzed with online SNPstats and SPSS25.0. Results Three genotypes were founded for all of the three loci. The frequencies of TT, GT and GG genotypes of rs7041 were 20(54.1%), 14(37.8%) and 3(8.1%) in the case group, and 44(44.4%), 41(41.4%) and 14(14.2%) in the control group, respectively. The frequencies of AA, AG and GG of rs222016 in the case group were 14(37.8%), 17(46.0%), 6(16.2%), and 34(34.3%), 39(39.4%), 26(26.3%) in the control group respectively. The frequencies of CC, AC and AA of rs4588 were 17(46.0%), 18(48.6%), 2(5.4%) in the case group, and 59(59.6%), 33(33.3%), 7(7.1%) in the control group. There were no statistical differences between the two groups(P>0.05).Conclusions The genetic differences of three polymorphisms of GC gene, including rs7041, rs222016 and rs4588, might not be associated with the elderly patients with sinusitis in Hainan province.
引文
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8 李雪盛,于晓红.成人慢性鼻窦炎伴息肉型患者血清25-羟维生素D水平变化及与病变程度的相关性观察[J].中国实验诊断学,2017;21(12):2065-8.
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11 Van CK,Zhang N,Gevaert P,et al.Pathogenesis of chronic rhinosinusitis:inflammation[J].Curr Allergy Asthma Rep,2006;6(6):487-94.
12 Akbar NA,Zacharek MA.Vitamin D:immunomodulation of asthma,allergic rhinitis,and chronic rhinosinusitis[J].Curr Opin Otolaryngol Head Neck Surg,2011;19(3):224-8.
13 Delanghe JR,Speeckaert R,Speeckaert MM.Behind the scenes of vitamin D binding protein:more than vitamin D binding[J].Best Pract Res Clin Endocrinol Metab,2015;29(5):773-86.
14 Speeckaert MM,Speeckaert R,Van GN,et al.Vitamin D binding protein:a multifunctional protein of clinical importance[M].Amsterdam:Elsevier Science & Technology,2014:1-57.
15 Nakayama T,Okada N,Yoshikawa M,et al.Assessment of suitable reference genes for RT-qPCR studies in chronic rhinosinusitis[J].Sci Rep,2018;8(1):1-9.
16 Ganz AB,Park H,Malysheva OV,et al.Vitamin D binding protein rs7041 genotype alters vitamin D metabolism in pregnant women[J].FASEB J,2017;32(4):2012-20.
17 Weintraub SJ,Fleckenstein JF,Marion TN,et al.Vitamin D-binding protein gene polymorphisms may contribute to the racial disparity in genotype 1 chronic hepatitis C treatment outcome[J].Hepatology,2013;58(5):1864.
18 Navasnazario A,Li FY,Shabanova V,et al.Effect of vitamin D binding protein (DBP) genotype on the development of asthma in children[J].Ann Allergy Asthma Immunol,2014;112(6):519-24.
2 王迪,张慧慧,文连姬,等.咽喉反流与老年患者耳鼻喉科疾病相关性研究进展[J].中国老年学杂志,2018;38(15):3816-9.
3 Rittschof CC,Pattanaik S,Johnson L,et al.European position paper on rhinosinusitis and nasal polyps 2012:updates and highlights on diagnosis and treatment of rhinosinusitis[J].Med J Chin PLA,2013;38(2):87-93.
4 Hamilos DL.Chronic rhinosinusitis:epidemiology and medical management[J].J Allergy Clin Immunol,2011;128(4):693-707.
5 Testa D,Marcuccio G,Panin G,et al.Nasal mucosa healing after endoscopic sinus surgery in chronic rhinosinusitis of elderly patients:role of topic alpha-tocopherol acetate[J].Aging Clin Exp Res,2017;29(Suppl 1):1-5.
6 张彩萍,苏会璇.老年糖尿病伴慢性鼻窦炎鼻息肉36例[J].湖北医药学院学报,2010;29(4):360-1.
7 Christensen JM,Cheng J,Earls P,et al.Vitamin D pathway regulatory genes encoding 1α-hydroxylase and 24-hydroxylase are dysregulated in sinonasal tissue during chronic rhinosinusitis[J].Int Forum Allergy Rhinol,2017;7(2):169-76.
8 李雪盛,于晓红.成人慢性鼻窦炎伴息肉型患者血清25-羟维生素D水平变化及与病变程度的相关性观察[J].中国实验诊断学,2017;21(12):2065-8.
9 Stokes PJ,Rimmer J.The relationship between serum vitamin D and chronic rhinosinusitis:a systematic review[J].Am J Rhinol Allergy,2016;30(1):23-8.
10 Carlberg C,Campbell MJ.Vitamin D receptor signaling mechanisms:Integrated actions of a well-defined transcription factor[J].Steroids,2013;78(2):127-36.
11 Van CK,Zhang N,Gevaert P,et al.Pathogenesis of chronic rhinosinusitis:inflammation[J].Curr Allergy Asthma Rep,2006;6(6):487-94.
12 Akbar NA,Zacharek MA.Vitamin D:immunomodulation of asthma,allergic rhinitis,and chronic rhinosinusitis[J].Curr Opin Otolaryngol Head Neck Surg,2011;19(3):224-8.
13 Delanghe JR,Speeckaert R,Speeckaert MM.Behind the scenes of vitamin D binding protein:more than vitamin D binding[J].Best Pract Res Clin Endocrinol Metab,2015;29(5):773-86.
14 Speeckaert MM,Speeckaert R,Van GN,et al.Vitamin D binding protein:a multifunctional protein of clinical importance[M].Amsterdam:Elsevier Science & Technology,2014:1-57.
15 Nakayama T,Okada N,Yoshikawa M,et al.Assessment of suitable reference genes for RT-qPCR studies in chronic rhinosinusitis[J].Sci Rep,2018;8(1):1-9.
16 Ganz AB,Park H,Malysheva OV,et al.Vitamin D binding protein rs7041 genotype alters vitamin D metabolism in pregnant women[J].FASEB J,2017;32(4):2012-20.
17 Weintraub SJ,Fleckenstein JF,Marion TN,et al.Vitamin D-binding protein gene polymorphisms may contribute to the racial disparity in genotype 1 chronic hepatitis C treatment outcome[J].Hepatology,2013;58(5):1864.
18 Navasnazario A,Li FY,Shabanova V,et al.Effect of vitamin D binding protein (DBP) genotype on the development of asthma in children[J].Ann Allergy Asthma Immunol,2014;112(6):519-24.
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