菊茎叶提取物改善肠功能失调的代谢组学研究
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摘要
目的整体评价菊茎叶水提取物(JST)和乙醇提取物(JCT)对肠功能失调的改善作用及其机制。方法应用脂多糖(LPS)制备兔肠功能失调模型,给予JST和JCT,应用1H-NMR技术研究兔粪便内源性代谢物变化规律。结果相比对照组,模型组乳酸盐、甲酸盐显著增加,丙酸盐、谷氨酰胺、谷氨酸盐、天冬氨酸等显著下降;JST和JCT均能够增加天冬氨酸、腺嘌呤、苯丙氨酸、酪氨酸水平,同时降低甲酸盐水平。结论代谢通路分析表明JST和JCT通过调节LPS所致紊乱的苯丙氨酸、酪氨酸和色氨酸生物合成,丙氨酸、天冬氨酸和谷氨酸盐代谢,以及甘氨酸、丝氨酸和苏氨酸代谢,发挥改善肠道功能紊乱的作用。
Objective To holistically evaluate the protective effects of water and ethanol extracts of stems and leaves of Chrysanthemum morifolium and its underlying mechanism. Methods Gut dysfunction model was established by injection of liposaccharide in rabbits, and administrated with water and ethanol extracts of stems and leaves of C. morifolium to investigate the treatment. Feces of rabbits in each group were collected and analyzed by 1 H-NMR complemented with multivariate statistical methods to evaluate the metabolic alteration. Results Compared with the control group, the levels of lactate and formate in liposaccharide intoxicated model group were significantly increased, and the concentrations of propionate, glutamine, glutamate, aspartate were notably decreased. Both the water and ethanol extracts of stems and leaves of C. morifolium ameliorated gut dysfunction of rabbits in a similar manner, increased the decreased levels of aspartate, adenine, phenylalanine, tyrosine induced by liposaccharide, and reduced the elevated content of formate. Conclusion Pathway analysis revealed that both the water and ethanol extracts of stems and leaves of C. morifolium could regulate the disturbed phenylalanine, tyrosine and tryptophan biosynthesis; disordered alanine, aspartate and glutamate metabolism and imbalanced glycine, serine and threonine amino acid metabolism, exerting a holistic protective effect on gut disorder. Thus, this study lays a scientific foundation for the resource utilization of stems and leaves of C. morifolium after the harvest of the inflorescence.
Objective To holistically evaluate the protective effects of water and ethanol extracts of stems and leaves of Chrysanthemum morifolium and its underlying mechanism. Methods Gut dysfunction model was established by injection of liposaccharide in rabbits, and administrated with water and ethanol extracts of stems and leaves of C. morifolium to investigate the treatment. Feces of rabbits in each group were collected and analyzed by 1 H-NMR complemented with multivariate statistical methods to evaluate the metabolic alteration. Results Compared with the control group, the levels of lactate and formate in liposaccharide intoxicated model group were significantly increased, and the concentrations of propionate, glutamine, glutamate, aspartate were notably decreased. Both the water and ethanol extracts of stems and leaves of C. morifolium ameliorated gut dysfunction of rabbits in a similar manner, increased the decreased levels of aspartate, adenine, phenylalanine, tyrosine induced by liposaccharide, and reduced the elevated content of formate. Conclusion Pathway analysis revealed that both the water and ethanol extracts of stems and leaves of C. morifolium could regulate the disturbed phenylalanine, tyrosine and tryptophan biosynthesis; disordered alanine, aspartate and glutamate metabolism and imbalanced glycine, serine and threonine amino acid metabolism, exerting a holistic protective effect on gut disorder. Thus, this study lays a scientific foundation for the resource utilization of stems and leaves of C. morifolium after the harvest of the inflorescence.
引文
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[24]李园,常相伟,朱邵晴,等.黄芪花营养成分分析与资源价值评价[J].中草药,2018,49(18):4408-4416.
[25]郭盛,唐仁茂,段金廒,等.从黄蜀葵花制药过程副产物中提取得到的花蜡、蜜糖、黄酮部位及其制备方法和应用技术:中国,CN105832579A[P].2018-08-31.
[26]陶小芳,沈飞,宿树兰,等.生脉注射液生产过程五味子药渣中资源性物质的分析与循环利用途径探讨[J].中草药,2015,46(18):2712-2719.
[27]沈飞,宿树兰,江曙,等.丹红注射液生产过程中丹参固体废弃物的资源性成分分析及其转化机制研究[J].中草药,2015,46(16):2471-2476.
[2]Liu Y,Wang Z,Zhang J.Dietary Chinese Herbs[M].Vienna:Springer,2015.
[3]常相伟,魏丹丹,陈栋杰,等.药用与茶用菊花资源形成源流与发展变化[J].中国现代中药,2019,21(1):116-123.
[4]魏丹丹,常相伟,郭盛,等.菊花及菊资源开发利用及资源价值发现策略[J].中国现代中药,2019,21(1):37-44.
[5]朱琳,郭建明,杨念云,等.菊非药用部位化学成分的分布及其动态积累研究[J].中草药,2014,45(3):425-431.
[6]Lai J P,Lim Y H,Su J,et al.Identification and characterization of major flavonoids and caffeoylquinic acids in three Compositae plants by LC/DAD-APCI/MS[J].J Chromatogr B,2007,848(2):215-225.
[7]段金廒,张伯礼,宿树兰,等.基于循环经济理论的中药资源循环利用策略与模式探讨[J].中草药,2015,46(12):1715-1722.
[8]段金廒,宿树兰,郭盛,等.中药资源化学研究与资源循环利用途径及目标任务[J].中国中药杂志,2015,40(17):3395-3401.
[9]Tao J H,Duan J A,Jiang S,et al.Polysaccharides from Chrysanthemum morifolium Ramat ameliorate colitis rats by modulating the intestinal microbiota community[J].Oncotarget,2017,8(46):80790-80803.
[10]Tao J H,Duan J A,Jiang S,et al.Simultaneous determination of six short-chain fatty acids in colonic contents of colitis mice after oral administration of polysaccharides from Chrysanthemum morifolium Ramat by gas chromatography with flame ionization detector[J].J Chromatogr B-Anal Technol Biomed Life Sci,2016,doi:10.1016/j.jchromb.2016.07.002.
[11]刘新丽,卢弘.内毒素诱导的葡萄膜炎发病机制的研究进展[J].国际眼科纵览,2016,40(4):283-288.
[12]Wei D D,Ge D,Guo P P,et al.A bird’s eye view of anisatin induced convulsive seizures in brain by a1H-NMR based metabolic approach[J].Mol Biosyst,2014,10(11):2923-2934.
[13]Wei D D,Wang J S,Li M H,et al.A pilot study of the onset of hepatic encephalopathy(OHE)in mice induced by thioacetamide and the protective effect of taurine by holistic metabolic characterization[J].Metabolomics,2015,11(3):559-570.
[14]Wei D D,Wang J S,Duan J A,et al.Metabolomic assessment of acute cholestatic injuries induced by thioacetamide and by bile duct ligation,and the protective effects of Huang-Lian-Jie-Du-Decoction[J].Front Pharmacol,2018,9:458.
[15]Xing Y X,Li M H,Tao L,et al.Anti-cancer effects of emodin on HepG2 cells as revealed by 1H-NMR based metabolic profiling[J].J Proteome Res,2018,17(5):1943-1952.
[16]Ruan L Y,Fan J T,Hong W,et al.Isoniazid-induced hepatotoxicity and neurotoxicity in rats investigated by1H-NMR based metabolomics approach[J].Toxicol Lett,2018,295:256-269.
[17]Wei D D,Wang J S,Wang P R,et al.Toxic effects of chronic low-dose exposure of thioacetamide on rats based on NMR metabolic profiling[J].J Pharm Biomed Anal,2014,doi:10.1016/j.jpba.2014.05.035.
[18]Nicholson J K,Holmes E,Kinross J,et al.Host-gut microbiota metabolic interactions[J].Science,2012,336(6086):1262-1267.
[19]Dodd D,Spitzer M H,Van Treuren W,et al.A gut bacterial pathway metabolizes aromatic amino acids into nine circulating metabolites[J].Nature,2017,551(7682):648-652.
[20]Kim C H,Park J,Kim M.Gut microbiota-derived short-chain fatty acids,T cells,and inflammation[J].Immun Network,2014,14(6):277-288.
[21]沙秀秀,宿树兰,沈飞,等.不同生长期丹参茎叶及花序中丹酚酸类化学成分的分布与积累动态分析评价[J].中草药,2015,46(22):3414-3419.
[22]沙秀秀,戴新新,宿树兰,等.丹参茎叶药材的质量标准研究[J].药物分析杂志,2016,36(6):1094-1100.
[23]刘杰,郭盛,段金廒,等.黄蜀葵花期不同组织器官中多类型资源性化学成分的分析与利用价值挖掘[J].中国中药杂志,2016,41(20):3782-3791.
[24]李园,常相伟,朱邵晴,等.黄芪花营养成分分析与资源价值评价[J].中草药,2018,49(18):4408-4416.
[25]郭盛,唐仁茂,段金廒,等.从黄蜀葵花制药过程副产物中提取得到的花蜡、蜜糖、黄酮部位及其制备方法和应用技术:中国,CN105832579A[P].2018-08-31.
[26]陶小芳,沈飞,宿树兰,等.生脉注射液生产过程五味子药渣中资源性物质的分析与循环利用途径探讨[J].中草药,2015,46(18):2712-2719.
[27]沈飞,宿树兰,江曙,等.丹红注射液生产过程中丹参固体废弃物的资源性成分分析及其转化机制研究[J].中草药,2015,46(16):2471-2476.