响应面法优选泽兰多糖的大孔吸附树脂纯化工艺
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摘要
目的:考察8种大孔吸附树脂D3520、H103、HPD-100、HPD-700、AB-8、HPD722、S-8、HPD-600对泽兰多糖的纯化效果,以Box-Behnken法优化最佳大孔吸附树脂的最优纯化工艺.方法:以多糖保留率、脱色率、脱蛋白率的加权综合评分为指标,考察大孔树脂、洗脱流速、上样浓度、洗脱剂用量对纯化结果的影响,通过Box-Behnken设计建立响应面模型来优选大孔树脂泽兰多糖的工艺参数.结果:优选的泽兰多糖的大孔树脂纯化工艺为:取HPD-100大孔吸附树脂,泽兰多糖的上样质量浓度为0.03 g/mL,洗脱流速为1.1 mL/min,洗脱体积为40 mL,以此优选工艺纯化后,多糖保留率69.21%,脱色率60.24%,蛋白脱除率75.67%.结论:泽兰多糖的纯化工艺稳定可靠,HPD-100大孔吸附树脂纯化工艺效果良好,适合工业化生产.
Objective:To investigate the effect of 8 kinds of macroporous adsorption resins, including D3520, H103, HPD-100, HPD-700, AB-8, HPD722, S-8 and HPD-600, on purification of Lycopus lucidus polysaccharide and to optimize the process of purification by Box-Behnken design using the best macroporous adsorption resin. Methods: The influence of macroporous resin type, elution velocity, sample loading concentration, volume of eluent on the purification effect were evaluated with weighted comprehensive score of the rate of polysaccharide retention, decoloration and protein removal. Response surface model was established by Box-Behnken design to optimize the process parameters for purification of Lycopus lucidus polysaccharide with macroporous resin. Results: The optimized purification process and results for Lycopus lucidus polysaccharide with macroporous resin was as follows. Using HPD-100 macroporous resin for separation, the sample loading concentration was 0.03 g/mL, the elution velocity was 1.1 mL/min and the elution volume was 40 mL. Using this process, a polysaccharide retention rate of 69.21%, a decoloration rate of 60.24% and a protein removal rate of 75.67% was achieved. Conclusion:The developed purification process of Lycopus lucidus polysaccharides is stable and reliable. The purification process using HPD-100 macroporous adsorption resin generates good effect and is suitable for industrial production.
Objective:To investigate the effect of 8 kinds of macroporous adsorption resins, including D3520, H103, HPD-100, HPD-700, AB-8, HPD722, S-8 and HPD-600, on purification of Lycopus lucidus polysaccharide and to optimize the process of purification by Box-Behnken design using the best macroporous adsorption resin. Methods: The influence of macroporous resin type, elution velocity, sample loading concentration, volume of eluent on the purification effect were evaluated with weighted comprehensive score of the rate of polysaccharide retention, decoloration and protein removal. Response surface model was established by Box-Behnken design to optimize the process parameters for purification of Lycopus lucidus polysaccharide with macroporous resin. Results: The optimized purification process and results for Lycopus lucidus polysaccharide with macroporous resin was as follows. Using HPD-100 macroporous resin for separation, the sample loading concentration was 0.03 g/mL, the elution velocity was 1.1 mL/min and the elution volume was 40 mL. Using this process, a polysaccharide retention rate of 69.21%, a decoloration rate of 60.24% and a protein removal rate of 75.67% was achieved. Conclusion:The developed purification process of Lycopus lucidus polysaccharides is stable and reliable. The purification process using HPD-100 macroporous adsorption resin generates good effect and is suitable for industrial production.
引文
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[9] GAURAV DWIVEDI,PUNEET VERMA,M. P. SHARMA. Optimization of Storage Stability for Karanja Biodiesel Using Box-Behnken Design[J]. Waste and Biomass Valorization,2018,9(4):645-655.
[10] 赖红芳,黄秀香,石祖玲. 超声波辅助提取翠云草中穗花杉双黄酮工艺的响应面优化[J]. 食品研究与开发,2018,39(13):47-51.
[11] YANG S Y, LI Y, JIA D Y, et al. The synergy of Box-Behnken designs on the optimization of polysaccharide extraction from mulberry leaves[J]. Industrial Crops and Products, 2017, 99(1):70-78.
[12] 綦菲,王昶,李轶,等. 单因素考察活性炭脱除麦冬多糖色素[J]. 中国实验方剂学杂志,2011,17(18):38-40. QI F, WANG C, LI Y, et al. Decolorization technology of polysaccharide from ophiopogonis radix with activated carbon by single factor test[J]. Chinese Journal of Experimental Traditional Medical Formulae,2011,17(18):38-40.
[13] 张骆琪,刘苏伟,王飞,等.地黄多糖超声提取工艺优化及其抗氧化活性[J]. 中成药,2018,40(12):2662-2667. ZHANG L Q, LIU S W, WANG F, et al. Optimization of ultrasonic extraction technology and antioxidant activity of rehmanniae radix polysaccharide[J]. Chinese Traditional Patent Medicine, 2018,40(12):2662-2667.
[14] 徐中香,胡浩,李季楠,等. 青稞麸皮阿拉伯木聚糖的提取工艺优化及结构分析[J]. 食品科学,2018,39(8):191-197. XU Z X, HU H, LI J N, et al. Optimization of extraction process and structural analysis of arabinoxylans from hull-Less barely bran[J]. Food Science,2018,39(8):191-197.
[15] 蒋林,米阿娜,罗宇东,等. 星点设计-响应面法优选龙钻通痹方总生物碱大孔树脂纯化工艺[J]. 中草药,2018,49(2):337-345. JIANG L, MI A N, LUO Y D, et al. Optimization of purification process for total alkaloids from longzuan tongbi recipe with macroporous resin by central composite design and response surface method[J]. Chinese Traditional and Herbal Drugs,2018,49(2):337-345.
[16] 徐华容,李丽琪,刘阳,等. AB-8大孔树脂纯化紫色马铃薯花色苷的工艺研究[J]. 中国药学杂志,2017,52(24):2141-2145. XU H R, LI L Q, LIU Y, et al. Purification of anthocyanin from purple potato by ab-8 macroporous resin[J]. Chinese Pharmaceutical Journal,2017,52(24):2141-2145.
[17] 粟敏,汤婷,唐玮蔚,等. 大孔树脂富集纯化黄蜀葵花总黄酮的工艺研究[J]. 中药材,2017,40(6):1402-1405. LI M, TANG T, TANG W W, et al. Study on the purification and enrichment of total flavonoids from Abelmoschus manihot by macroporous resin[J]. Journal of Chinese Medicinal Materials,2017,40(6):1402-1405.
[18] 张春燕,吴美儒,周毅峰,等. 利用大孔树脂对莼菜多糖脱色的工艺优化[J]. 食品工业科技,2018,39(15):154-157,164. ZHANG C Y, WU M R, ZHOU Y F, et al. Optimization of decolorization process for Brasenia schreberi polysaccharide by macroporous resin[J]. Science and Technology of Food Industry,2018,39(15):154-157,164.
[2] 任强,王红玲,周学刚,等. 泽兰的化学成分、质量分析及药理作用研究进展[J]. 中国药房,2015,26(18):2588-2592. REN Q, WANG H L, ZHOU X G, et al. Advances in the study of chemical composition, quality analysis and pharmacological action of Lycopi Herba[J]. China Pharmacy,2015,26(18):2588-2592.
[3] LI C, LI Z L,WANG T,et al. Chemical constituents of the aerial parts of lycopus lucidus var. hirtus[J]. Chemistry of Natural Compounds,2014,50(2):288-290.
[4] 刘富岗,冯素香,刘炯,等. 泽兰多糖对D-半乳糖致衰老小鼠的抗氧化作用[J].中成药,2018,40(8):1833-1836. LIU F G, FENG S X, LIU J, et al. Antioxidant effect of Lycopi Herba polysaccharide on aging mice induced by D-galactose[J]. Chinese Traditional Patent Medicine, 2018,40(8):1833-1836.
[5] 刘富岗,冯素香,刘炯,等. 泽兰多糖酶提工艺的优化[J].中成药,2018,40(6):1395-1398. LIU F G, FENG S X, LIU J, et al. Optimization of enzymatic extraction technology of Lycopi Herba polysaccharide[J]. Chinese Traditional Patent Medicine, 2018,40(6):1395-1398.
[6] LI W K,WANG K,JIANG N F,et al. Antioxidant and antihyperlipidemic activities of purified polysaccharides from Ulva pertusa[J]. Journal of Applied Phycology, 2018,30(4):2619-2627.
[7] Delphine R N, Jeffrey F, Alicia N, et al. Immunogenicity of the meningococcal polysaccharide conjugate vaccine in pediatric kidney transplant patients[J]. Pediatric Nephrology, 2018, 33(6):1037-1043.
[8] 刘富岗,冯素香,张杰,等. 白屈菜多糖纯化工艺的优化[J]. 中成药,2018,40(2):475-477. LIU F G, FENG S X, ZHANG J, et al. Optimization of purification process of Chelidoni Herba polysaccharide[J]. Chinese Traditional Patent Medicine, 2018,40(2):475-477.
[9] GAURAV DWIVEDI,PUNEET VERMA,M. P. SHARMA. Optimization of Storage Stability for Karanja Biodiesel Using Box-Behnken Design[J]. Waste and Biomass Valorization,2018,9(4):645-655.
[10] 赖红芳,黄秀香,石祖玲. 超声波辅助提取翠云草中穗花杉双黄酮工艺的响应面优化[J]. 食品研究与开发,2018,39(13):47-51.
[11] YANG S Y, LI Y, JIA D Y, et al. The synergy of Box-Behnken designs on the optimization of polysaccharide extraction from mulberry leaves[J]. Industrial Crops and Products, 2017, 99(1):70-78.
[12] 綦菲,王昶,李轶,等. 单因素考察活性炭脱除麦冬多糖色素[J]. 中国实验方剂学杂志,2011,17(18):38-40. QI F, WANG C, LI Y, et al. Decolorization technology of polysaccharide from ophiopogonis radix with activated carbon by single factor test[J]. Chinese Journal of Experimental Traditional Medical Formulae,2011,17(18):38-40.
[13] 张骆琪,刘苏伟,王飞,等.地黄多糖超声提取工艺优化及其抗氧化活性[J]. 中成药,2018,40(12):2662-2667. ZHANG L Q, LIU S W, WANG F, et al. Optimization of ultrasonic extraction technology and antioxidant activity of rehmanniae radix polysaccharide[J]. Chinese Traditional Patent Medicine, 2018,40(12):2662-2667.
[14] 徐中香,胡浩,李季楠,等. 青稞麸皮阿拉伯木聚糖的提取工艺优化及结构分析[J]. 食品科学,2018,39(8):191-197. XU Z X, HU H, LI J N, et al. Optimization of extraction process and structural analysis of arabinoxylans from hull-Less barely bran[J]. Food Science,2018,39(8):191-197.
[15] 蒋林,米阿娜,罗宇东,等. 星点设计-响应面法优选龙钻通痹方总生物碱大孔树脂纯化工艺[J]. 中草药,2018,49(2):337-345. JIANG L, MI A N, LUO Y D, et al. Optimization of purification process for total alkaloids from longzuan tongbi recipe with macroporous resin by central composite design and response surface method[J]. Chinese Traditional and Herbal Drugs,2018,49(2):337-345.
[16] 徐华容,李丽琪,刘阳,等. AB-8大孔树脂纯化紫色马铃薯花色苷的工艺研究[J]. 中国药学杂志,2017,52(24):2141-2145. XU H R, LI L Q, LIU Y, et al. Purification of anthocyanin from purple potato by ab-8 macroporous resin[J]. Chinese Pharmaceutical Journal,2017,52(24):2141-2145.
[17] 粟敏,汤婷,唐玮蔚,等. 大孔树脂富集纯化黄蜀葵花总黄酮的工艺研究[J]. 中药材,2017,40(6):1402-1405. LI M, TANG T, TANG W W, et al. Study on the purification and enrichment of total flavonoids from Abelmoschus manihot by macroporous resin[J]. Journal of Chinese Medicinal Materials,2017,40(6):1402-1405.
[18] 张春燕,吴美儒,周毅峰,等. 利用大孔树脂对莼菜多糖脱色的工艺优化[J]. 食品工业科技,2018,39(15):154-157,164. ZHANG C Y, WU M R, ZHOU Y F, et al. Optimization of decolorization process for Brasenia schreberi polysaccharide by macroporous resin[J]. Science and Technology of Food Industry,2018,39(15):154-157,164.