磷霉素降解菌株的筛选及其降解性能研究
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摘要
以磷霉素为唯一碳源,从驯化污泥中纯化筛选出3株降解磷霉素的菌株(F1、F2、F3),对其进行形态学、生理生化特征以及16S r DNA序列分析,鉴定其菌属,分析其生长特征;选用生长适应期较短的菌株F3开展不同p H和温度条件下菌株对磷霉素降解性能的研究,并用一级动力学方程对其降解过程进行拟合。结果表明:菌株F1鉴定为微杆菌属(Microbacterium sp.),菌株F2和F3均鉴定为贪铜菌属(Cupriavidus sp.)。菌株F3对磷霉素的降解性能受温度和p H的影响,在种液接种量为20%、转速为150 r/min、磷霉素浓度为20 mg/L时,菌株F3对磷霉素的最适降解条件:p H为5. 0,温度为20℃。菌株F3对磷霉素的降解符合一级动力学特征,拟合方程相关系数(R~2)> 0. 88; p H为5. 0、温度为20℃时,半衰期(t1/2)为31. 36 d。
Fosfomycin-degradation strains were isolated from acclimated activated sludge feeding fosfomycin as the sole carbon source. Three fosfomycin-degradation strains, named F1, F2 and F3, were screened out and their morphological observation, physiological and biochemical characteristics, as well as 16 S r DNA sequence were analysed to identify the genus and the growth characteristics. Strain F3 had a faster growth adaptation period, and was selected to study the degradation of fosfomycin. The effects of operation parameters such as temperature and p H on fosfomycin degradation were explored, and the degradation process was fitted by the first-order kinetic equation.The results showed that strain F1 belonged to Microbacterium sp. and strain F2 and F3 both belonged to Cupriavidus sp. The degradation process of fosfomycin of strain F3 was affected by temperature and p H. The optimal conditions were pH 5. 0 and temperature 20 ℃ when the inoculum was 20%, the rotation speed was 150 r/min, and the fosfomycin concentration was 20 mg/L. The degradation process fitted well with pseudo-first-order kinetic model,and the correlation coefficient R~2 exceeded 0. 88. When pH was 5. 0 and temperature was 20 ℃, the half-life period was 31. 36 d.
Fosfomycin-degradation strains were isolated from acclimated activated sludge feeding fosfomycin as the sole carbon source. Three fosfomycin-degradation strains, named F1, F2 and F3, were screened out and their morphological observation, physiological and biochemical characteristics, as well as 16 S r DNA sequence were analysed to identify the genus and the growth characteristics. Strain F3 had a faster growth adaptation period, and was selected to study the degradation of fosfomycin. The effects of operation parameters such as temperature and p H on fosfomycin degradation were explored, and the degradation process was fitted by the first-order kinetic equation.The results showed that strain F1 belonged to Microbacterium sp. and strain F2 and F3 both belonged to Cupriavidus sp. The degradation process of fosfomycin of strain F3 was affected by temperature and p H. The optimal conditions were pH 5. 0 and temperature 20 ℃ when the inoculum was 20%, the rotation speed was 150 r/min, and the fosfomycin concentration was 20 mg/L. The degradation process fitted well with pseudo-first-order kinetic model,and the correlation coefficient R~2 exceeded 0. 88. When pH was 5. 0 and temperature was 20 ℃, the half-life period was 31. 36 d.
引文
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[10]CS N,BAGI Z,RKHELY G,et al. Bioaugmentation of biogas production by a hydrogen-producing bacterium[J]. Bioresource Technology,2015,186:286-293.
[11] HERRERO M,STUCKEY D C. Bioaugmentation and its application in wastewater treatment:a review[J]. Chemosphere,2015,140:119-128.
[12] WANG R,ZHENG P,ZHANG M,et al. Bioaugmentation of nitrate-dependent anaerobic ferrous oxidation by heterotrophic denitrifying sludge addition:a promising way for promotion of chemoautotrophic denitrification[J]. Bioresource Technology,2015,197:410-415.
[13] CARACCIOLO A B,TOPP E,GRENNI P. Pharmaceuticals in the environment:biodegradation and effects on natural microbial communiti:a review[J]. Journal of Pharmaceutical and Biomedical Analysis,2015,106:25-36.
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[19]廖苗.磷霉素制药废水的生物强化处理及应用研究[D].北京:中国环境科学研究院,2017.
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[22] National Center for Biotechnology Information. Basic local alignment search tool[EB/OL].[2018-08-20]. https://blast.ncbi. nlm. nih. gov/Blast. cgi
[23]顾夏声.废水生物处理数学模式[M]. 2版.北京:清华大学出版社,1993.
[24]孙瑞珠,马玉龙,张娟,等.泰乐菌素降解菌的筛选及其降解动力学研究[J].中国环境科学,2013,33(4):722-727.SUN R Z,MA Y L,ZHANG J,et al. Isolation and degradation dynamics of a tylosin-degrading strain[J]. China Environmental Science,2013,33(4):722-727.
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[28]杨正中,吴广,金文,等.异菌脲降解菌YJN-G的分离、鉴定及降解特性[J].应用与环境生物学报,2017,23(1):164-168.YANG Z Z,WU G,JIN W,et al. Isolation,identification and characterization of an iprodione-degrading strain YJN-G[J].Chinese Journal of Applied and Environmental Biology,2017,23(1):164-168.
[29]杨洋,邵宗泽.印度洋深海沉积物石油烃降解菌分离、鉴定与多样性分析[J].生物资源,2017,39(6):423-433.YANG Y,SHAO Z Z. Isolation,characterization and diversity of hydrocarbon-degrading bacteria in deep-sea sediments of Indian Ocean[J]. Biotic Resources,2017,39(6):423-433.
[30]徐天宇,胡苏莹,周峻岗,等.微杆菌Microbacterium sp.FY1538降解赤霉烯酮的活性研究[J].复旦学报(自然科学版),2016,55(2):223-231.XU T Y,HU S Y,ZHOU J G,et al. Analysis of zearalenone-degrading activities of Microbacterium sp. FY1538[J]. Journal of Fudan University(Natural Science),2016,55(2):223-231.
[31]沈娥. Cupriavidus sp. SHE的筛选及特性研究[D].大连:大连理工大学,2015.
[32]吴志国.硝基苯降解菌的分离鉴定、降解途径与关键酶基因的克隆及其细胞固定化研究[D].南京:南京农业大学,2011.
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