三种含氮杂环化合物对蚕豆根尖细胞的诱导作用
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摘要
选取喹啉、吲哚和吡啶三种典型的含氮杂环化合物(NHCs)作为目标物质,研究其对蚕豆根尖细胞的诱导作用,通过测定微核率和脂质过氧化作用,判断不同浓度的三种物质对细胞遗传毒性的影响。实验结果表明,当喹啉、吲哚以及吡啶作用于蚕豆根尖细胞时,呈现不同程度的诱导作用和污染损伤,在喹啉、吲哚和吡啶浓度分别为20 mg/L、5 mg/L、20 mg/L时,微核率达到最大值,对应的微核指数分别为15.15、9.09和6.06,相应的脂质过氧化作用也达到最大,丙二醛含量(MDA)分别为0.019μmol/g,0.023μmol/g和0.018μmol/g,三种物质对蚕豆根尖细胞有明显的遗传毒性作用。
Three nitrogen-containing heterocyclic compounds(NHCs) of quinoline,indole and pyridine were selected as the target material to study their inducement effect on the Vicia faba root tip cells.The effects of different concentrations of three substances on genetic toxicity were determined by the effects of micronucleus rate and lipid peroxidation.The results indicated that quinoline,indole and pyridine showed different degrees of pollution damage and inducement effect when they were applied to Vicia faba root tip cells.When the concentrations of quinoline,indole and pyridine were 20 mg/L,5 mg/L and 20 mg/L,respectively,the micronucleus rate reached the maximum,and the corresponding micronucleus indexes were 15.15,9.09 and 6.06,respectively,the corresponding degree of lipid peroxidation reached the maximum,the MDA values at 0.019 mol/L,0.023 mol/L and 0.018 mol/L,respectively.Three substances have obvious genetic toxicity on Vicia faba root tip cells.
Three nitrogen-containing heterocyclic compounds(NHCs) of quinoline,indole and pyridine were selected as the target material to study their inducement effect on the Vicia faba root tip cells.The effects of different concentrations of three substances on genetic toxicity were determined by the effects of micronucleus rate and lipid peroxidation.The results indicated that quinoline,indole and pyridine showed different degrees of pollution damage and inducement effect when they were applied to Vicia faba root tip cells.When the concentrations of quinoline,indole and pyridine were 20 mg/L,5 mg/L and 20 mg/L,respectively,the micronucleus rate reached the maximum,and the corresponding micronucleus indexes were 15.15,9.09 and 6.06,respectively,the corresponding degree of lipid peroxidation reached the maximum,the MDA values at 0.019 mol/L,0.023 mol/L and 0.018 mol/L,respectively.Three substances have obvious genetic toxicity on Vicia faba root tip cells.
引文
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[3]Padoley K V,Mudliar S N,Pandey R A.Heterocyclic nitrogenous pollutants in the environment and their treatment options.An overview[J].Bioresource Technology,2008,99(10):4029-4043.
[4]刘通.不同结构咪唑类离子液体在土壤中对蚕豆幼苗的生态毒性及结构对其毒性的影响[D].山东农业大学,2016.
[5]周璐,刘永军,刘羽,等.兰炭废水中酚类有机物的去除及遗传毒性分析[J].水处理技术,2017,43(2):102-106.
[6]常青.邻苯二甲酸二(2-乙基己基)酯对蚕豆幼苗发育的影响[D].华中师范大学,2010.
[7]邓冰,全旖,穆龙.氘水对蚕豆根尖的诱变效应[J].环境科学,2017,36(3):508-513.
[8]王超,段链,张宏伟,等.甲醛单一及复合污染对蚕豆根尖细胞微核的影响及污染评价[J].癌变·畸变·突变,2016,28(5):383-387.
[9]乐知,王沁.应用蚕豆根尖微核技术检测洗发水的安全性状况[J].安全与环境工程,2011,18(2):81-84+92.
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[11]陈凯峰,邓妍,郭建军,等.吲哚乙酸,萘乙酸,对蚕豆根尖细胞微核的诱导作用[J].华中师范大学学报(自然科学版),2011,45(4):607-611.
[12]王兰君,王金花,朱鲁生,等.4种典型PPCPs对蚕豆和大蒜根尖细胞微核率的影响[J].环境科学,2016,37(4):1568-1575.
[13]赖金龙,陶宗娅,吴国,等.邻苯二甲酸二丁酯对蚕豆胚根细胞微核形成的影响及毒理机制[J].生态毒理学报,2014,9(1):107-113.
[14]杜研,王康宇,王义,等.真菌诱导子对黄芪愈伤组织生长和代谢的影响[J].吉林农业大学学报,2011,33(3):293-300.
[15]林仁亨,黄海涛,李焕秀,等.银杏外种皮对生菜生长及生理特性的影响[J].土壤,2018,50(4):732-737.
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