细鳞斜颌鲴5种组织2种同工酶的电泳分析
|
摘要
为了解细鳞斜颌鲴(Xenocypris microlepis)的生化遗传特征,并以此为基础为其种质资源鉴定提供理论依据,本研究采用不连续聚丙烯酰胺凝胶垂直板电泳对细鳞斜颌鲴5种组织(心脏、晶状体、肌肉、肾脏和肝脏)的乳酸脱氢酶(LDH)和苹果酸脱氢酶(MDH)进行分析。结果表明:所测样本鱼的心脏、晶状体、肌肉、肾脏和肝脏中的LDH酶带数分别为:6～8、5、2～4、5和4～6条; MDH酶带数分别为:7～8、5～6、7～8、3～4和4条;细鳞斜颌鲴的LDH同工酶和MDH同工酶具有组织特异性,其心脏、肾脏和肝脏MDH分为上清液型(s-MDH)和线粒体型(m-MDH),肌肉和晶状体组织中未见上清液型MDH(s-MDH)。研究认为晶状体组织LDH可作为鉴定细鳞斜颌鯝种质特征的生化遗传参数。本研究可为鉴定细鳞斜颌鲴种质、制定其种质标准提供参考依据。
This paper was to study the morphological and biochemical genetics characteristics of Xenocypris microlepis so as to provide theoretical basis for their germplasm resources identification. By using the vertical polyacrylamide gel electrophoresis,lactate dehydrogenase( LDH) and malate dehydrogenase( MDH) isozymes from five tissues( heart,eye,muscle,kidney and liver) were analyzed. The results showed that: the band numbers of LDH isozymes expressed in heart,eye,muscle,kidney and liver were 6-8,5,2-4,5 and 4-6,respectively,and that of MDH were 7-8,5-6,7-8,3-4 and 4,respectively. These two kinds of isozymes from Xenocypris microlepis presented tissue specificity. Briefly,MDH isozymes expressed in heart,kidney and liver of Xenocypris microlepis were divided into supernatant type( s-MDH) and mitochondria type( m-MDH). The supernatant type of malate dehydrogenase( MDH) isozymes could not be detected in muscle and eyes. And lactate dehydrogenase( LDH) isozymes expressed in eyes can be used as the biochemical genetic marker of Xenocypris microlepis. This research provided a reference basis for the establishment of the germplasm standards and the germplasm identification of Xenocypris microlepis.
This paper was to study the morphological and biochemical genetics characteristics of Xenocypris microlepis so as to provide theoretical basis for their germplasm resources identification. By using the vertical polyacrylamide gel electrophoresis,lactate dehydrogenase( LDH) and malate dehydrogenase( MDH) isozymes from five tissues( heart,eye,muscle,kidney and liver) were analyzed. The results showed that: the band numbers of LDH isozymes expressed in heart,eye,muscle,kidney and liver were 6-8,5,2-4,5 and 4-6,respectively,and that of MDH were 7-8,5-6,7-8,3-4 and 4,respectively. These two kinds of isozymes from Xenocypris microlepis presented tissue specificity. Briefly,MDH isozymes expressed in heart,kidney and liver of Xenocypris microlepis were divided into supernatant type( s-MDH) and mitochondria type( m-MDH). The supernatant type of malate dehydrogenase( MDH) isozymes could not be detected in muscle and eyes. And lactate dehydrogenase( LDH) isozymes expressed in eyes can be used as the biochemical genetic marker of Xenocypris microlepis. This research provided a reference basis for the establishment of the germplasm standards and the germplasm identification of Xenocypris microlepis.
引文
[1]王宏伟,王安利,王维娜,等.鱼类同工酶研究进展[J].动物学报,2001,47:101-105.
[2] Markert,C L,Ursprung H. Developmental Genetic[M].New Jersey:Prentice-Hall Inc,1971:214.
[3]徐木生,王福庭,石身清,等.道观河水库细鳞斜颌鲴自然增殖研究[J].水利渔业,1991(1):13-16.
[4]王银东,熊邦喜,王明学,等.细鳞斜颌鲴的生物学特性与资源利用[J].水利渔业,2002,22(3):45-47.
[5]乔德亮,李思发.细鳞斜颌鲴三个群体形态差异[J].生态学杂志,2010,29(12):2425-2430.
[6]夏重志,姜作发,刘忠泽,等.镜泊湖细鳞斜颌鲴的年龄与生长[J].水产学报,1992,16(1):60-66.
[7]孙莉,唐明虎,杨庭玉,等.细鳞斜颌鲴人工繁殖技术要点[J].科学养鱼,2015(1):8-9.
[8]李康,李渝成,周密,等.中国鲤科鱼类染色体组型的研究Ⅱ.鲴亚科四种鱼的染色体组型[J].动物学报,1983,29(3):207-213.
[9]乔德亮,何晓梅,韦传宝,等.细鳞斜颌鲴三个群体线粒体COⅡ的遗传变异[J].淡水渔业,2011,41(5):17-21.
[10]韩现芹,宋文平,姜巨峰,等.雌雄细鳞斜颌鲴不同部位蛋白质营养价值的比较与评价[J].广东海洋大学学报,2013,33(3):33-40.
[11]叶忠平,吕业坚,林岗.细鳞斜颌鲴仔鱼饥饿试验与不可逆点的确定[J].南方农业学报,2012,43(4):536-539.
[12]徐万土,戴海平,程顺,等.细鳞斜颌鲴精子活力观察[J].浙江海洋学院学报,2013,32(4):309-314.
[13]朱蓝菲.几种鲤科鱼类及杂种的乳酸脱氢酶同工酶的比较[J].水生生物学集刊,1982,7(4):539-545.
[14]曹丽琴,孟庆闻.中国鲴亚科鱼类同工酶和骨骼特征及系统演化的探讨(鲤形目:鲤科)[J].动物分类学报,1992,17(3):366-376.
[15]杨品红,徐黎明,王志陶,等.细鳞斜颌鲴不同组织中LDH同工酶的比较研究[J].激光生物学报,2009,18(4):509-515.
[16]张林,周剑光,张涛,等.长江水系细鳞斜颌鲴形态特征及生化遗传特性分析[J].中国渔业质量与标准,2018,8(2):29-35.
[17]贺刚,方春林,王伟萍,等.大口鲇(♀)×怀头鲇(♂)杂种F1的形态学及遗传学研究[J].湖北农业科学,2011,50(23):4916-4919.
[18]张涛,陈建武,张林,等.赤眼鳟形态特征及其两种同工酶的组织特异性分析[J].南方农业学报,2017,48(12):2281-2287.
[19]贺刚,何力,方春林,等.中华草龟(♀)与中华花龟(♂)及其杂种F1代染色体及同工酶比较[J].淡水渔业,2012,42(2):3-9.
[20] Shaklee J B,Allendorf F W,Morizot D C,et al. Genetic nomenclature for protein-coding loci in fish[J]. Trans Amer Fish Soc,1990,119:2-15.
[21]熊全沫.同工酶电泳数据的分析及其在种群遗传上的应用[J].遗传,1986,8(1):1-5.
[22]蒋晓华,昝瑞光,谷甦.云南滇池高背鲫鱼乳酸脱氢酶(LDH)同工酶发育遗传学分析[J].西南农业学报,1996,9(4):53-57.
[23]金建丽,杨春文,金志民.刺猬乳酸脱氢酶和超氧化物歧化酶同工酶的研究[J].中国林副特产,2005(3):42-44.
[24]唐章生.巴西鲷乳酸脱氢酶同工酶的初步研究[J].水利渔业,2001,21(4):14-16.
[25]李娴,朱永安,孟庆磊,等.澳洲长鳍鳗4种同工酶的组织特异性研究[J].水利渔业,2001,21(4):14-16.
[26]龙华,邓怀,邹世平.欧洲鳗与日本鳗的同工酶鉴别研究[J].湖北农学院学报,2000,20(1):68-71.
[27]胡思玉,彭垠,赵海涛,等.四川裂腹鱼同工酶研究[J].湖北农业科学,2015,54(18):4540-4543.
[28] Holbrook J,Liljas A,Sreindel S J,et al. Lactate dehydrogenase[M]. New York:Academic Press, 1975:191-291.
[29]王红叶,张娟,蔡焰值,等.白甲鱼不同组织同工酶的组织特异性研究[J].湖北农业科学,2011,50(23):4912-4915.
[30]薛国雄,官平,张燕生,等.草鱼LDH同工酶比较酶学和免疫化学性质[J].水产学报,1992,16(4):357-364.
[31]汪新颖,王波,候松涛,等.苹果酸脱氢酶的结构及功能[J].生物学杂志,2009,26(4):69-72.
[32]杨书婷,桂建芳.两个雌核发育白鲢群体同工酶分析及遗传标记的确定[J].水生生物学报,1999,23(3):264-268.
[33]余来宁,夏小平,杨东,等.草鱼♀×鱤♂杂交F1代同工酶和蛋白质的电泳分析[J].安徽农业科学,2014,42(30):10573-10575.
[34]扈廷茂,刘明秋,王焕来,等.黄河鲤和德国镜鲤及其杂交种F1同工酶的比较研究[J].内蒙古大学学报(自然科学版),1994,25(3):305-312.
[35]余敏,杨正荣,曾琳,等.云南高背鲫鱼不同组织同工酶分析[J].动物学杂志,2006,41(6):97-103.
[36]贾守菊,应雪萍,陈艳乐,等.中华绒螯蟹不同发育阶段腹肢粘液腺同工酶的比较[J].海洋湖沼通报,2004(4):52-60.
[37]薛俊增.患"抖抖病"中华绒螯蟹可溶性蛋白质和同工酶分析[J].动物学杂志,2002,37(2):17-21.
[38]陈姝君.我国斑点叉尾鮰种质资源的分子遗传学研究[D].大连:辽宁师范大学,2008.
[2] Markert,C L,Ursprung H. Developmental Genetic[M].New Jersey:Prentice-Hall Inc,1971:214.
[3]徐木生,王福庭,石身清,等.道观河水库细鳞斜颌鲴自然增殖研究[J].水利渔业,1991(1):13-16.
[4]王银东,熊邦喜,王明学,等.细鳞斜颌鲴的生物学特性与资源利用[J].水利渔业,2002,22(3):45-47.
[5]乔德亮,李思发.细鳞斜颌鲴三个群体形态差异[J].生态学杂志,2010,29(12):2425-2430.
[6]夏重志,姜作发,刘忠泽,等.镜泊湖细鳞斜颌鲴的年龄与生长[J].水产学报,1992,16(1):60-66.
[7]孙莉,唐明虎,杨庭玉,等.细鳞斜颌鲴人工繁殖技术要点[J].科学养鱼,2015(1):8-9.
[8]李康,李渝成,周密,等.中国鲤科鱼类染色体组型的研究Ⅱ.鲴亚科四种鱼的染色体组型[J].动物学报,1983,29(3):207-213.
[9]乔德亮,何晓梅,韦传宝,等.细鳞斜颌鲴三个群体线粒体COⅡ的遗传变异[J].淡水渔业,2011,41(5):17-21.
[10]韩现芹,宋文平,姜巨峰,等.雌雄细鳞斜颌鲴不同部位蛋白质营养价值的比较与评价[J].广东海洋大学学报,2013,33(3):33-40.
[11]叶忠平,吕业坚,林岗.细鳞斜颌鲴仔鱼饥饿试验与不可逆点的确定[J].南方农业学报,2012,43(4):536-539.
[12]徐万土,戴海平,程顺,等.细鳞斜颌鲴精子活力观察[J].浙江海洋学院学报,2013,32(4):309-314.
[13]朱蓝菲.几种鲤科鱼类及杂种的乳酸脱氢酶同工酶的比较[J].水生生物学集刊,1982,7(4):539-545.
[14]曹丽琴,孟庆闻.中国鲴亚科鱼类同工酶和骨骼特征及系统演化的探讨(鲤形目:鲤科)[J].动物分类学报,1992,17(3):366-376.
[15]杨品红,徐黎明,王志陶,等.细鳞斜颌鲴不同组织中LDH同工酶的比较研究[J].激光生物学报,2009,18(4):509-515.
[16]张林,周剑光,张涛,等.长江水系细鳞斜颌鲴形态特征及生化遗传特性分析[J].中国渔业质量与标准,2018,8(2):29-35.
[17]贺刚,方春林,王伟萍,等.大口鲇(♀)×怀头鲇(♂)杂种F1的形态学及遗传学研究[J].湖北农业科学,2011,50(23):4916-4919.
[18]张涛,陈建武,张林,等.赤眼鳟形态特征及其两种同工酶的组织特异性分析[J].南方农业学报,2017,48(12):2281-2287.
[19]贺刚,何力,方春林,等.中华草龟(♀)与中华花龟(♂)及其杂种F1代染色体及同工酶比较[J].淡水渔业,2012,42(2):3-9.
[20] Shaklee J B,Allendorf F W,Morizot D C,et al. Genetic nomenclature for protein-coding loci in fish[J]. Trans Amer Fish Soc,1990,119:2-15.
[21]熊全沫.同工酶电泳数据的分析及其在种群遗传上的应用[J].遗传,1986,8(1):1-5.
[22]蒋晓华,昝瑞光,谷甦.云南滇池高背鲫鱼乳酸脱氢酶(LDH)同工酶发育遗传学分析[J].西南农业学报,1996,9(4):53-57.
[23]金建丽,杨春文,金志民.刺猬乳酸脱氢酶和超氧化物歧化酶同工酶的研究[J].中国林副特产,2005(3):42-44.
[24]唐章生.巴西鲷乳酸脱氢酶同工酶的初步研究[J].水利渔业,2001,21(4):14-16.
[25]李娴,朱永安,孟庆磊,等.澳洲长鳍鳗4种同工酶的组织特异性研究[J].水利渔业,2001,21(4):14-16.
[26]龙华,邓怀,邹世平.欧洲鳗与日本鳗的同工酶鉴别研究[J].湖北农学院学报,2000,20(1):68-71.
[27]胡思玉,彭垠,赵海涛,等.四川裂腹鱼同工酶研究[J].湖北农业科学,2015,54(18):4540-4543.
[28] Holbrook J,Liljas A,Sreindel S J,et al. Lactate dehydrogenase[M]. New York:Academic Press, 1975:191-291.
[29]王红叶,张娟,蔡焰值,等.白甲鱼不同组织同工酶的组织特异性研究[J].湖北农业科学,2011,50(23):4912-4915.
[30]薛国雄,官平,张燕生,等.草鱼LDH同工酶比较酶学和免疫化学性质[J].水产学报,1992,16(4):357-364.
[31]汪新颖,王波,候松涛,等.苹果酸脱氢酶的结构及功能[J].生物学杂志,2009,26(4):69-72.
[32]杨书婷,桂建芳.两个雌核发育白鲢群体同工酶分析及遗传标记的确定[J].水生生物学报,1999,23(3):264-268.
[33]余来宁,夏小平,杨东,等.草鱼♀×鱤♂杂交F1代同工酶和蛋白质的电泳分析[J].安徽农业科学,2014,42(30):10573-10575.
[34]扈廷茂,刘明秋,王焕来,等.黄河鲤和德国镜鲤及其杂交种F1同工酶的比较研究[J].内蒙古大学学报(自然科学版),1994,25(3):305-312.
[35]余敏,杨正荣,曾琳,等.云南高背鲫鱼不同组织同工酶分析[J].动物学杂志,2006,41(6):97-103.
[36]贾守菊,应雪萍,陈艳乐,等.中华绒螯蟹不同发育阶段腹肢粘液腺同工酶的比较[J].海洋湖沼通报,2004(4):52-60.
[37]薛俊增.患"抖抖病"中华绒螯蟹可溶性蛋白质和同工酶分析[J].动物学杂志,2002,37(2):17-21.
[38]陈姝君.我国斑点叉尾鮰种质资源的分子遗传学研究[D].大连:辽宁师范大学,2008.