结直肠癌石蜡切片行免疫组化染色后的DNA质量评估
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摘要
目的对免疫组化染色后的结直肠癌石蜡标本重新处理,提取DNA并进行质量评估,旨在探讨稀缺标本重利用进行相关分子检测的可行性。方法收集病理确诊的结直肠癌石蜡标本30例,其中结肠癌13例,直肠癌17例。切片采用免疫组化En Vision两步法染色后进行DNA提取,并进行CTNNB1基因检测和CDH1甲基化检测。结果 30例经免疫组化染色的石蜡标本中提取到的DNA质量合格,并可以顺利进行后续CTNNB1基因检测和CDH1甲基化检测。结论结直肠癌标本,尤其是标本量较少或难以获取的样本,经免疫组化染色后仍能够提取高质量的DNA,并且可以用于靶基因的下游分子分析,是值得推广的方法。
Objective Explore the feasibility of reusing immunohistochemical staining sections to extract DNA and to analyses the feasibility of molecular detection of these samples. Methods A total of 30 cases of colorectal cancer paraffin specimens were collected from the Department of Pathology,Nanjing General Hospital of Nanjing Military Command from June 2017 to September 2017,including 13 cases of colon cancer and 17 cases of rectal cancer. DNA samples were extracted by immunohistochemical En Vision two-step staining sections,and CTNNB1 gene detection and CDH1 methylation were detected. Results The quality of DNA extracted from 30 immunohistochemically stained paraffin specimens was qualified,and the subsequent CTNNB1 gene detection and CDH1 methylation assay were successfully performed.Conclusion Colorectal cancer specimens,especially those with small or difficult specimens,can extract high-quality DNA even if after immunohistochemical staining and can be used for downstream molecular analysis of target genes. It is a technical method worthy of popularization.
Objective Explore the feasibility of reusing immunohistochemical staining sections to extract DNA and to analyses the feasibility of molecular detection of these samples. Methods A total of 30 cases of colorectal cancer paraffin specimens were collected from the Department of Pathology,Nanjing General Hospital of Nanjing Military Command from June 2017 to September 2017,including 13 cases of colon cancer and 17 cases of rectal cancer. DNA samples were extracted by immunohistochemical En Vision two-step staining sections,and CTNNB1 gene detection and CDH1 methylation were detected. Results The quality of DNA extracted from 30 immunohistochemically stained paraffin specimens was qualified,and the subsequent CTNNB1 gene detection and CDH1 methylation assay were successfully performed.Conclusion Colorectal cancer specimens,especially those with small or difficult specimens,can extract high-quality DNA even if after immunohistochemical staining and can be used for downstream molecular analysis of target genes. It is a technical method worthy of popularization.
引文
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