基于不同倍性谷子主茎形态建成的相关内源激素调控差异分析
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摘要
为揭示谷子相关内源激素对主茎形态发生及株型构建的影响,以晋谷21号(DJG21)及其同源四倍体诱变株(TJG21)为试验材料,采用高效液相色谱-质谱(HPLC-MS)联用技术,研究拔节期不同倍性谷子主茎内源激素IAA、GAs、ABA、SA、JA的动态差异及其对主茎形态建成的影响。结果表明,DJG21和TJG21成株主茎节间数相同,均为15。DJG21和TJG21分别在播后67和75 d拔节完成。拔节前期TJG21主茎增长较DJG21快,后期二者无显著差异。DJG21染色体经诱变加倍后,TJG21主茎的ABA、JA、SA含量明显增加。TJG21主茎拔节与节间增长滞后与其ABA含量增高及(IAA+GAs)/ABA值降低密切相关。TJG21主茎SA及JA含量整体大于DJG21,前期JA含量增加更为明显,后期SA含量增加更为明显。DJG21和TJG21拔节期主茎内源激素含量及调控的差异造成不同倍性谷子生长发育进程的不同,是由遗传及环境因素双重影响下的形态发生过程。本研究结果不仅为人为构建激素平衡以调控谷子生长发育提供了一定的理论依据,也为不同倍性谷子拔节期转录组分析及相关基因发掘奠定了基础。
To reveal the influences of endogenous hormones on main stem morphogenesis and plant architecture formation millet, Jingu 21(DJG21) and its autotetraploid mutagenesis strain(TJG21) as the experimental materials, and high performance liquid chromatography-mass spectrometry(HPLC-MS) was carrired out to detect the dynamic differences of endogenous hormones in stem, including IAA, GAs, ABA, SA, JA, and its influence on main stem morphogenesis between two millet strains with different ploidy in elongation stage. The results showed that DJG21 and TJG21 have equal internodes, both were 15. It took 67 d for DJG21 stem to finish jointing, while TJG21 was 75 d. The main stem of TJG21 growed faster than that of DJG21 in early elongation stage. But there was no significant difference between them in later stage. The contents of ABA, JA and SA in the main stem of TJG21 increased obviously after chromosomes of DJG21 was mutated and doubled. The elongation hysteresis of TJG21 main stem and slow growth of its internodes were closely related to the increase of ABA content and the decrease of(IAA+GAs)/ABA value. As a whole, the contents of SA and JA in the main stem of TJG21 were higher than those in DJG21, and the increase of JA was more obvious in the early stage, while SA was more obvious in the later stage. The differences of endogenous hormone content and regulation between DJG21 and TJG21 during elongating stage leaded to different growth and development processes of millet with different ploidy, which is aprocess of morphogenesis involved with both genetic and environment factors. The results of this study not only provide a theoretical basis for the artificial construction of hormone balance to regulate the growth and development of millet, but also lay a foundation for transcriptional analysis of millet with different ploidy at jointing stage and identification of candidate genes.
To reveal the influences of endogenous hormones on main stem morphogenesis and plant architecture formation millet, Jingu 21(DJG21) and its autotetraploid mutagenesis strain(TJG21) as the experimental materials, and high performance liquid chromatography-mass spectrometry(HPLC-MS) was carrired out to detect the dynamic differences of endogenous hormones in stem, including IAA, GAs, ABA, SA, JA, and its influence on main stem morphogenesis between two millet strains with different ploidy in elongation stage. The results showed that DJG21 and TJG21 have equal internodes, both were 15. It took 67 d for DJG21 stem to finish jointing, while TJG21 was 75 d. The main stem of TJG21 growed faster than that of DJG21 in early elongation stage. But there was no significant difference between them in later stage. The contents of ABA, JA and SA in the main stem of TJG21 increased obviously after chromosomes of DJG21 was mutated and doubled. The elongation hysteresis of TJG21 main stem and slow growth of its internodes were closely related to the increase of ABA content and the decrease of(IAA+GAs)/ABA value. As a whole, the contents of SA and JA in the main stem of TJG21 were higher than those in DJG21, and the increase of JA was more obvious in the early stage, while SA was more obvious in the later stage. The differences of endogenous hormone content and regulation between DJG21 and TJG21 during elongating stage leaded to different growth and development processes of millet with different ploidy, which is aprocess of morphogenesis involved with both genetic and environment factors. The results of this study not only provide a theoretical basis for the artificial construction of hormone balance to regulate the growth and development of millet, but also lay a foundation for transcriptional analysis of millet with different ploidy at jointing stage and identification of candidate genes.
引文
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[30] 豆峻岭.多倍体西瓜番茄红素合成关键酶基因表达及相关内源激素研究[D].北京:中国农业科学院,2014:31-32
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[34] 王熹,施一平.水稻生长发育的化学调节技术[J].植物生理学通讯,1980,4(2):9-13
[35] 陆雯,潘璐琪,王雪艳.水杨酸及茉莉酸介导植物抗病性的研究进展[J].贵州农业科学,2013,41(10):40-43
[2] Santner A,Calderon-Villalobos L Ⅰ A,Estelle M.Plant hormones are versatile chemical regulators of plant growth[J].Nature Chemical Biology,2009,5(5):301-307
[3] 熊国胜,李家洋,王永红.植物激素调控研究进展[J].科学通报,2009,54(18):2718-2733
[4] 常莉,薛建平.生长素极性运输研究进展[J].生物学杂志,2008,25(6):9-13
[5] Carrier D J,Abu Bakar N T,Swarup R,Callaghan R,Napier R M,Bennett M J,Kerr Ⅰ D.The Binding of auxin to the Arabidopsis auxin influx transporter AUX1[J].Plant Physiology,2008,148(1):529-535
[6] Tan X,Calderon-Villalobos L Ⅰ,Sharon M,Zheng C,Robinson C Ⅴ,Estelle M,Zheng N.Mechanism of auxin perception by the TIR1 ubiquitin ligase[J].Nature,2007,446(7136):640-645
[7] 杨艳会,张清哲,陈军营,陈新建.生长素结构与其活性关系的揭示[J].植物生理学通讯,2009,45(11):1119-1124
[8] 任怡怡,戴绍军,刘炜.生长素的运输及其在信号转导及植物发育中的作用[J].生物技术通报,2012(3):10-16
[9] Reinecke D M,Wickramarathna A D,Ozga J A,Kurepin L Ⅴ,Jin A L,Good A G,Pharis R P.Gibberellin 3-oxidase gene expression patterns in?uence gibberellin biosynthesis,growth,and development in pea[J].Plant Physiology,2013,163(6):929-945
[10] Eriksson S,B?hlenius H,Moritz T,Nilsson O.GA4 is the active gibberellin in the regulation of LEAFY transcription and Arabidopsis ?oral initiation[J].Plant Cell,2006,18(9):2172-2181
[11] Graeber K,Linkies A,Steinbrecher T,Mummenhoff K,Tarkowska D,Tureckova Ⅴ,Ignatz M,Sperber K,Voegele A,De J H,Urbanova T,Strnad M,Leubner-Metzger G.DELAY OF GERMINATION 1 mediates a conserved coat-dormancy mechanism for the temperature and gibberellin-dependent control of seed germination[J].Proceedings of the National Academy of Sciences of the United States of America,2014,111(34):3571-3580
[12] Olszewski N,Sun T P,Gubler F.Gibberellin signaling:Biosynthesis,catabolism,and response pathways[J].Plant Cell,2002,14(9):61-80
[13] Wang W,Zhang J,Qin Q,Yue J,Huang B,Xu X,Yan L,Hou S.The six conserved serine/threonine sites of REPRESSOR OF ga1-3 protein are important for its functionality and stability in gibberellin signaling in Arabidopsis[J].Planta,2014,240(4):763-779
[14] 潘瑞炽.植物生理学[M].北京:高等教育出版社,2012
[15] 丛庆,张琪,宋丽莉,陈月,蔡洪生,郭长虹.激素在植物冷胁迫应答中的角色[J].核农学报,2016,30(3):614-619
[16] Liang J H,Yang L X,Chen X,Li L,Guo L D,Li H H,Zhang B Y.Cloning and characterization of the promoter of the 9-cis-epoxycarotenoid dioxygenase gene in Arachis hypogaea L.[J].Bioscience,Biotechnology,and Biochemistry,2009,73(9):2103-2106
[17] Yoshida T,Mogami J,Yamaguchi-Shinozaki K.ABA-dependent and ABA-independent signaling in response to osmotic stress in and plants[J].Current Opinion in Plant Biology,2014,21(8):133-139
[18] Nakashima K,Yamaguchi-Shinozaki K,Shinozaki K.The transcriptional regulatory network in the drought response and its crosstalk in abiotic stress responses including drought,cold,and heat[J].Frontiers in Plant Science,2014,5(5):170-177
[19] Vergne E,Grand X,Ballini E,Ballini E,Chalvon Ⅴ,Saindrenan P,Tharreau D,Notteghem J L,Morel J B.Preformed expression of defense is a hallmark of partial resistance to rice blast fungal pathogen Magnaporthe oryzae[J].BMC Plant Biology,2010,10(1):206-207
[20] Brochmann C,Brysting A K,Alsos Ⅰ G,Borgen L,Grundt H H,Scheen A C,Elven R.Polyploidy in arctic plants[J].Biological Journal of the Linnean Society,2004,82(4):521-536
[21] Wendel J F.Genome evolution in polyploids[J].Plant Molecular Biology,2000,42(1):225-249
[22] 王涛,陈孟龙,刘玲,宁传丽,蔡斌华,章镇,乔玉山.植物多倍体化中基因组和基因表达的变化[J].植物学报,2015,50(4):504-515
[23] Jia X P,Zhang Z B,Liu Y H,Zhang C W,Shi Y S,Song Y C,Wang T Y,Li Y.Development and genetic mapping of SSR markers in foxtail millet(Setaria italica)[J].Theoretical and Applied Genetics,2009,118(4):821-829
[24] 王晓宇,王根全,王节之,郝晓芬,刘鑫,张正,常建忠,王雪梅.四倍体谷子细胞学鉴定及不同倍性谷子孕穗期光合因子日响应差异分析[J].植物遗传资源学报,2017,18(5):830-836
[25] 刘清,童建华,史齐,彭克勤,王若仲,蔺万煌,Mohammed Humayun Kabir,沈革志,萧浪涛.一个矮秆多分蘖水稻突变体的植物激素动态特性分析[J].中国农业科学,2014,47(13):2519-2528
[26] 罗林杰,曾健,田朝霞,赵忠.植物的发育:从细胞到个体[J].科学通报,2016,61(33):3532-3540
[27] 张立军,梁宗锁.植物生理学[M].北京:科学出版社,2007
[28] 宋平.水稻矮生性和开花的植物激素调控[D].南京:南京农业大学,2000:13-14
[29] Hoffmann-Benning S,Kende H.On the role of abscisic acid and gibberellin in the regulation of growth in rice[J].Plant Physiology,1992,99(2):1156-1161
[30] 豆峻岭.多倍体西瓜番茄红素合成关键酶基因表达及相关内源激素研究[D].北京:中国农业科学院,2014:31-32
[31] Niki T,Mitsuhara Ⅰ,Seo S,Ohtsubo N,Ohashi Y.Antagonistic effect of salicylic acid and jasmonic acid on the expression of pathogenesis-related(PR)protein genes in wounded mature tobacco leaves[J].Plant and Cell Physiology,1998,39(5):500-507
[32] Mason H S,Mullet J E.Expression of two soybean vegetative storage protein genes during development and in response to water deficit,wounding,and jasmonic acid[J].Plant Cell,1990,2(6):569-579
[33] 黄桃鹏,李媚娟,王睿,李玲.赤霉素生物合成及信号转导途径研究进展[J].植物生理报,2015,51(8):1241-1247
[34] 王熹,施一平.水稻生长发育的化学调节技术[J].植物生理学通讯,1980,4(2):9-13
[35] 陆雯,潘璐琪,王雪艳.水杨酸及茉莉酸介导植物抗病性的研究进展[J].贵州农业科学,2013,41(10):40-43