酒女贞子配方颗粒质量标准研究
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摘要
目的提高酒女贞子配方颗粒质量标准控制。方法采用薄层色谱法,以女贞子对照药材作为对照进行定性鉴别;采用高效液相色谱法测定其特征成分特女贞苷含量。结果薄层色谱鉴别中,供试品溶液色谱在与对照药材溶液色谱相应的位置上,显相同颜色的荧光斑点。特女贞苷在进样量为0. 766 46～15. 329 19μg范围内与峰面积线性关系良好(r2=0. 999 8);平均回收率为91. 70%,RSD为1. 14%(n=6)。结论该方法简便、准确,重复性良好,完善和提高了酒女贞子配方颗粒的质量标准。
Objective To improve the quality control standard of Wine-Fried Ligustri Lucidi Fructus Dispensing Granules. Methods TLC method was used for qualitative identification with Ligustri Lucidi Fructus as the reference crude drug,and HPLC method was used for content determination of specnuezhenide in Wine-Fried Ligustri Lucidi Fructus Dispensing Granules. Results In TLC identification,the fluorescence spots in the chromatogram obtained with the test solution corresponded in position and color with the spots in the chromatogram obtained with the reference crude drug solution. The specnuezhenide showed good linear relationship with peak area in the range of 0. 766 46-15. 329 19 μg( r2 = 0. 999 8),the average recovery rate was 91. 70% and RSD was 1. 14%( n = 6). Conclusion The method is simple,accurate and reproducible,which can improve the quality standard of Wine-Fried Ligustri Lucidi Fructus Dispensing Granules.
Objective To improve the quality control standard of Wine-Fried Ligustri Lucidi Fructus Dispensing Granules. Methods TLC method was used for qualitative identification with Ligustri Lucidi Fructus as the reference crude drug,and HPLC method was used for content determination of specnuezhenide in Wine-Fried Ligustri Lucidi Fructus Dispensing Granules. Results In TLC identification,the fluorescence spots in the chromatogram obtained with the test solution corresponded in position and color with the spots in the chromatogram obtained with the reference crude drug solution. The specnuezhenide showed good linear relationship with peak area in the range of 0. 766 46-15. 329 19 μg( r2 = 0. 999 8),the average recovery rate was 91. 70% and RSD was 1. 14%( n = 6). Conclusion The method is simple,accurate and reproducible,which can improve the quality standard of Wine-Fried Ligustri Lucidi Fructus Dispensing Granules.
引文
[1]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015:45-46.
[2]张金玲,王小虎,邓振涛,等.中药女贞子化学成分及药理作用研究进展[J].现代中西医结合杂志,2013,22(36):4100-4101.
[3]张兴辉,石力夫.中药女贞子化学成分的研究(Ⅰ)[J].第二军医大学学报,2004,25(3):333-334.
[4]石力夫,王鹏,陈海生,等.中药女贞子水溶性化学成分的研究[J].药学学报,1995,30(12):935-938.
[5]石力夫,曹颖瑛,陈海生,等.中药女贞子中水溶性成分二种新裂环环烯醚萜甙的分离和鉴定[J].药学学报,1997,32(6):442-446.
[6]赵资堂,张学兰,李慧芬,等.女贞子炮制过程中2种三萜类成分及水溶性低分子糖含量变化规律研究[J].辽宁中医杂志,2012,39(12):2454-2457.
[7]侯杰.女贞子炮制前后主要有效成分变化规律研究[D].济南:山东中医药大学,2009.
[8]张学兰,侯杰,李慧芬,等.炮制对女贞子中特女贞苷含量的影响[J].中药材,2009,32(4):498-500.
[9]王瑞芳,张学兰,鹿栋尧,等.女贞子不同工艺炮制品中红景天苷和酪醇含量比较[J].中成药,2009,31(1):85-88.
[10]周蔚昕,刘涛,徐玉玲,等.对《中药配方颗粒质量控制与标准制定技术要求(》征求意见稿)部分内容的探讨[J].时珍国医国药,2017,28(10):2491-2493.
[2]张金玲,王小虎,邓振涛,等.中药女贞子化学成分及药理作用研究进展[J].现代中西医结合杂志,2013,22(36):4100-4101.
[3]张兴辉,石力夫.中药女贞子化学成分的研究(Ⅰ)[J].第二军医大学学报,2004,25(3):333-334.
[4]石力夫,王鹏,陈海生,等.中药女贞子水溶性化学成分的研究[J].药学学报,1995,30(12):935-938.
[5]石力夫,曹颖瑛,陈海生,等.中药女贞子中水溶性成分二种新裂环环烯醚萜甙的分离和鉴定[J].药学学报,1997,32(6):442-446.
[6]赵资堂,张学兰,李慧芬,等.女贞子炮制过程中2种三萜类成分及水溶性低分子糖含量变化规律研究[J].辽宁中医杂志,2012,39(12):2454-2457.
[7]侯杰.女贞子炮制前后主要有效成分变化规律研究[D].济南:山东中医药大学,2009.
[8]张学兰,侯杰,李慧芬,等.炮制对女贞子中特女贞苷含量的影响[J].中药材,2009,32(4):498-500.
[9]王瑞芳,张学兰,鹿栋尧,等.女贞子不同工艺炮制品中红景天苷和酪醇含量比较[J].中成药,2009,31(1):85-88.
[10]周蔚昕,刘涛,徐玉玲,等.对《中药配方颗粒质量控制与标准制定技术要求(》征求意见稿)部分内容的探讨[J].时珍国医国药,2017,28(10):2491-2493.