鱼组织中双去甲氧基姜黄素、去甲氧基姜黄素和姜黄素含量的超高效液相色谱法测定
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摘要
建立了黄颡鱼、团头鲂和草鱼血浆、肌肉、肝脏、肾脏中双去甲氧基姜黄素、去甲氧基姜黄素和姜黄素同时测定的超高效液相色谱紫外检测法(UPLC-UV)。样品经乙腈(含0.01%乙酸)提取,无水硫酸钠除水,正己烷去脂等样品处理,在ACQUIT UPLC BEH C18色谱柱上分离,428 nm波长处测定。双去甲氧基姜黄素、去甲氧基姜黄素和姜黄素在0.01~5.00 mg/L浓度范围内呈良好的线性关系,相关系数(r2)分别为0.998 7,0.999 8和0.999 6。在空白鱼组织中进行0.025,0.05,0.50,1.00 mg/kg 4个水平的加标回收实验,3种待测组分的平均回收率为64.7%~102.2%,相对标准偏差为0.69%~10.8%。鱼组织中3种待测组分的检出限(LOD)均为0.010 mg/kg,定量下限(LOQ)均为0.025 mg/kg。应用该方法研究了姜黄素在团头鲂体内的药代动力学规律。
An original method for the simultaneous determination of bisdemethoxycurcumin,demethoxycurcumin and curcumin in plasma,muscle,liver and kidney of yellow catfish(Pelteobagrus fulvidraco),bluntnose black bream(Megalobrama amblycephala) and grass carp(Ctenopharyngodon idellus) was established by ultra high performance liquid chromatography with ultraviolet detection(UPLC-UV). The sample was extracted using acetonitrile containing 0. 01% acetic acid,and the moisture of sample was removed with anhydrous sodium sulfate,then n-hexane was used for removing fat in sample. After that,the sample prepared was separated on an ACQUIT UPLC BEH C18 column and determined at 428 nm. Good linearities were observed in the range of 0. 01-5. 00 mg/L,and the correlation coefficients for bisdemethoxycurcumin,demethoxycurcumin and curcumin were 0. 9987,0. 999 8 and 0. 999 6,respectively. The average recoveries of 3 analytes in fish tissues at the spiked level of 0. 025-1. 00 mg/kg ranged from 64. 7% to 102. 2%,with relative standard deviations(RSDs) of 0. 69%-10. 8%. The detection limits(LODs) and quantitation limits(LOQs) for 3analytes in fish tissues were all 0. 010 mg/kg and 0. 025 mg/kg,respectively. The presented method was firstly applied in the investigation on the pharmacokinetic profile of curcumin in bluntnose black bream.
An original method for the simultaneous determination of bisdemethoxycurcumin,demethoxycurcumin and curcumin in plasma,muscle,liver and kidney of yellow catfish(Pelteobagrus fulvidraco),bluntnose black bream(Megalobrama amblycephala) and grass carp(Ctenopharyngodon idellus) was established by ultra high performance liquid chromatography with ultraviolet detection(UPLC-UV). The sample was extracted using acetonitrile containing 0. 01% acetic acid,and the moisture of sample was removed with anhydrous sodium sulfate,then n-hexane was used for removing fat in sample. After that,the sample prepared was separated on an ACQUIT UPLC BEH C18 column and determined at 428 nm. Good linearities were observed in the range of 0. 01-5. 00 mg/L,and the correlation coefficients for bisdemethoxycurcumin,demethoxycurcumin and curcumin were 0. 9987,0. 999 8 and 0. 999 6,respectively. The average recoveries of 3 analytes in fish tissues at the spiked level of 0. 025-1. 00 mg/kg ranged from 64. 7% to 102. 2%,with relative standard deviations(RSDs) of 0. 69%-10. 8%. The detection limits(LODs) and quantitation limits(LOQs) for 3analytes in fish tissues were all 0. 010 mg/kg and 0. 025 mg/kg,respectively. The presented method was firstly applied in the investigation on the pharmacokinetic profile of curcumin in bluntnose black bream.
引文
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[3]Wang J B,Wu T X.Reservoir Fisheries(王进波,吴天星.水利渔业),2007,27(6):105-106.
[4]Shi H Q,Zhou Y K,Xie X H.Feed Res.(史合群,周永奎,谢晓晖.饲料研究),2013,(6):9-11.
[5]Zheng Q M,Chen X F,Wen X B.The 7th world Chinese Symposium on Fish and Shrimp.Beijing:Feed Research Institute of Chinese Academy(第七届华人鱼虾大会论文集.北京:中国农业科学院饲料研究所),2008.
[6]Niu S Y,Hao F G,Xu Q Y.J.Inst.Sci.Technol.:Nat.Sci.Ed.(牛生洋,郝峰鸽,许秋亚.河南科技学院学报:自然科学版),2008,36(4):58-61.
[7]Han G,Zhang Y C,Liu S Y.Northwest Pharm.J.(韩刚,张义春,刘士勇.西北药学杂志),2005,20(1):15-16.
[8]Zhao X,Yuan D,Wang Q L,Wang R J.Chin.J.Pharm.Anal.(赵欣,袁丹,王启隆,王瑞杰.药物分析杂志),2005,25(6):643-647.
[9]Zhang Y H,Zhang D,Wang Y,Cai D F,Sun J.Chin.Pharm.J.(张韻慧,张丹,王妍,蔡德富,孙杰.中国药学杂志),2009,44(18):1423-1425.
[10]Sun J,Sun Y T,Zhang Z Q.Chin.Hosp.Pharm.J.(孙静,孙艳涛,张振秋.中国医院药学杂志),2010,8:714-716.
[11]Cai Y,Song S,Xu J,Chen J W,Cui X B.Pharm.Clin.Res.(蔡颖,宋坤,徐杰,陈建伟,崔小兵.药学与临床研究),2014,22(6):502-505.
[12]Shi J J,Feng S X,Hao R,Miao Y Y,Liu Q,Wang H,Miao M S.Acta Chin.Med.(史晶晶,冯素香,郝蕊,苗艳艳,刘琦,王辉,苗明三.中医学报),2015,30(6):853-855.
[13]Liu B Q,Hu X Z,Wang Y C,Zhu B C,Ding L.J.Instrum.Anal.(刘保启,胡孝忠,王玉春,朱宝成,丁良.分析测试学报),2004,23(1):109-111.
[14]Han C,Zhu Z O,Liu B,Huang F Z,Lei X X,Shen Y.Chin.J.Pharm.Anal.(韩超,朱振瓯,刘滨,黄芙珍,雷新响,沈燕.药物分析杂志),2013,33(2):185-188.
[15]Zhang P P,Qian J,Yang F,Cheng Q,Chen J,Zhang Y Y.J.Instum.Anal.(张盼盼,钱疆,杨方,程权,陈健,张玉燕.分析测试学报),2013,32(9):1127-1130.
[16]Yang F,Zhang P P,Qian J,Chen J,Zhang Y Y.Chin.J.Food Hyg.(杨方,张盼盼,钱疆,陈健,张玉燕.中国食品卫生杂志),2014,26(6):558-562.
[17]Gu J P,Deng Y J,Wang H,Zhang W M,Wang M.J.Chengdu Med.Coll.(顾吉晋,邓英杰,王浩,张伟明,王敏.成都医学院学报),2009,4(4):241-244.