基于网络药理学探讨土茯苓治疗痛风的作用机制
|
摘要
目的运用网络药理学预测土茯苓抗痛风有效成分的作用靶点及通路。方法通过TCMSP数据库和Drugbank数据库获取土茯苓有效成分及作用靶点,并运用Cytoscape软件构建靶点间相互作用网络并进行交联分析,筛选出土茯苓治疗痛风的有效成分及作用靶点,采用MAS3.0生物分子功能系统获取通路的相关信息并制成靶点通路网络模型。结果预测得到土茯苓治疗痛风的有效成分11个和有效靶点39个,并推断其作用机制可能与脂肪细胞因子信号通路、ERbB信号通路,Toll样受体信号通路等有关,且MAPK1、RELA、PTGS2等靶点基因可能起着关键性的作用。结论基于网络药理学探讨了土茯苓治疗痛风多成分-多靶点-多通路的作用特点,为进一步开展土茯苓治疗痛风作用机制研究提供了新的思路和方法。
Objective To predict the targets and pathways for the main active components of Smilax glabra in the treatment of gout based on network pharmacology. Methods The active components and targets of the S. glabra were obtained by TCMSP database and Drugbank database. Furthemore, the interaction network among the targets was established by Cytoscape software. Meanwhile,crosslink analysis was performed to screen out the active components and potential targets. Finally, the information was obtained from the MAS 3.0 biomolecular function system, and then the target pathway network model was established. Results In this study, a total of 11 effective components and 39 effective targets were predicted, which related to adipocytokine signaling pathway, ERbB signaling pathway, and Toll like receptor signaling pathway. Among these pathways, MAPK1, RELA, PTGS2 genes may play a crucial role.Conclusion This study investigated the characteristics on multi-component, multi-target and multi-pathway of S. glabra, which provided a new idea and method for further study on anti-gout mechanism of S. glabra.
Objective To predict the targets and pathways for the main active components of Smilax glabra in the treatment of gout based on network pharmacology. Methods The active components and targets of the S. glabra were obtained by TCMSP database and Drugbank database. Furthemore, the interaction network among the targets was established by Cytoscape software. Meanwhile,crosslink analysis was performed to screen out the active components and potential targets. Finally, the information was obtained from the MAS 3.0 biomolecular function system, and then the target pathway network model was established. Results In this study, a total of 11 effective components and 39 effective targets were predicted, which related to adipocytokine signaling pathway, ERbB signaling pathway, and Toll like receptor signaling pathway. Among these pathways, MAPK1, RELA, PTGS2 genes may play a crucial role.Conclusion This study investigated the characteristics on multi-component, multi-target and multi-pathway of S. glabra, which provided a new idea and method for further study on anti-gout mechanism of S. glabra.
引文
[1]范九梅,马卓.土茯苓药学研究概述[J].安徽农业科学,2018,46(8):36-37.
[2]杨秀飞,黄传兵,徐慧敏,等.大黄治疗痛风的作用机制研究进展[J].湖北中医杂志,2018,40(4):54-57.
[3]Major T J,Dalbeth N,Stahl E A,et al.An update on the genetics of hyperuricaemia and gout[J].Nat Rev Rheumatol,2018,14(6):341-353.
[4]Mahon O R,Dunne A.Disease-associated particulates and joint inflammation,mechanistic insights and potential therapeutic targets[J].Front Immuno,2018,doi:10.3389/fimmu.2018.01145.
[5]严诗楷,赵静,窦圣姗,等.基于系统生物学与网络生物学的现代中药复方研究体系[J].中国天然药物,2009,7(4):249-259.
[6]张晓囡,张军平,徐士欣,等.基于网络药理学的黄连解毒汤治疗高血压潜在机制研究[J].中草药,2018,49(24):5865-5875.
[7]Zhang Y Q,Mao X,Guo Q Y,et al.Network pharmacology-based approaches capture essence of Chinese herbal medicines[J].Chin Herb Med,2016,8(2):107-116.
[8]吴嘉瑞,蔺梦娟,刘鑫馗.基于网络药理学的“金银花-板蓝根”药对作用机制研究[J].中国医院用药评价与分析,2018,18(1):12-17.
[9]Rahimifard M,Manayi A,Baeeri M,et al.Investigation of beta-sitosterol and prangol extracted from Achillea tenoifolia along with whole root extract on isolated rat pancreatic islets[J].Iran J Pharm Res,2018,17(1):317-325.
[10]Deng S S,Wei T,Tan K L,et al.Phytosterol content and the campesterol:sitosterol ratio influence cotton fiber development:role of phytosterols in cell elongation[J].Sci China Life Sci,2016,59(2):183-193.
[11]Zaidun N H,Thent Z C,Latiff A A.Combating oxidative stress disorders with citrus flavonoid:Naringenin[J].Life Sci,2018,doi:10.1016/j.lfs.2018.07.017.
[12]Yadav M,Parle M,Jindal D K,et al.Protective effects of stigmasterol against ketamine-induced psychotic symptoms:Possible behavioral,biochemical and histopathological changes in mice[J].Pharmacol Rep,2018,70(3):591-599.
[13]Zhang J,Liu H,Song C,et al.Astilbin ameliorates pulmonary fibrosis via blockade of Hedgehog signaling pathway[J].Pulm Pharmacol Ther,2018,doi:10.1016/j.pupt.2018.03.006.
[14]赵鑫,苟小军,陈龙,等.花旗松素预防CCl4致大鼠急性肝损伤的代谢组学研究[J].上海中医药杂志,2017,51(8):82-86.
[15]Guo X,Ding X.Dioscin suppresses the viability of ovarian cancer cells by regulating the VEGFR2 and PI3K/AKT/MAPK signaling pathways[J].Oncol Lett,2018,15(6):9537-9542.
[16]Ruiz-Miyazawa K W,Staurengo-Ferrari L,Mizokami SS,et al.Quercetin inhibits gout arthritis in mice:Induction of an opioid-dependent regulation of inflammasome[J].Inflammopharmacology,2017,doi:10.1007/s10787-017-0356-x.
[17]Kong Q,Zhang S,Liang C,et al.LncRNA XISTfunctions as a molecular sponge of miR-194-5p to regulate MAPK1 expression in hepatocellular carcinoma cell[J].J Cell Biochem,2018,119(6):4458-4468.
[18]Tian B,Widen S G,Yang J,et al.The NFκB subunit RELA is a master transcriptional regulator of the committed epithelial-mesenchymal transition in airway epithelial cells[J].J Biol Chem,2018,293(42):16528-16545.
[2]杨秀飞,黄传兵,徐慧敏,等.大黄治疗痛风的作用机制研究进展[J].湖北中医杂志,2018,40(4):54-57.
[3]Major T J,Dalbeth N,Stahl E A,et al.An update on the genetics of hyperuricaemia and gout[J].Nat Rev Rheumatol,2018,14(6):341-353.
[4]Mahon O R,Dunne A.Disease-associated particulates and joint inflammation,mechanistic insights and potential therapeutic targets[J].Front Immuno,2018,doi:10.3389/fimmu.2018.01145.
[5]严诗楷,赵静,窦圣姗,等.基于系统生物学与网络生物学的现代中药复方研究体系[J].中国天然药物,2009,7(4):249-259.
[6]张晓囡,张军平,徐士欣,等.基于网络药理学的黄连解毒汤治疗高血压潜在机制研究[J].中草药,2018,49(24):5865-5875.
[7]Zhang Y Q,Mao X,Guo Q Y,et al.Network pharmacology-based approaches capture essence of Chinese herbal medicines[J].Chin Herb Med,2016,8(2):107-116.
[8]吴嘉瑞,蔺梦娟,刘鑫馗.基于网络药理学的“金银花-板蓝根”药对作用机制研究[J].中国医院用药评价与分析,2018,18(1):12-17.
[9]Rahimifard M,Manayi A,Baeeri M,et al.Investigation of beta-sitosterol and prangol extracted from Achillea tenoifolia along with whole root extract on isolated rat pancreatic islets[J].Iran J Pharm Res,2018,17(1):317-325.
[10]Deng S S,Wei T,Tan K L,et al.Phytosterol content and the campesterol:sitosterol ratio influence cotton fiber development:role of phytosterols in cell elongation[J].Sci China Life Sci,2016,59(2):183-193.
[11]Zaidun N H,Thent Z C,Latiff A A.Combating oxidative stress disorders with citrus flavonoid:Naringenin[J].Life Sci,2018,doi:10.1016/j.lfs.2018.07.017.
[12]Yadav M,Parle M,Jindal D K,et al.Protective effects of stigmasterol against ketamine-induced psychotic symptoms:Possible behavioral,biochemical and histopathological changes in mice[J].Pharmacol Rep,2018,70(3):591-599.
[13]Zhang J,Liu H,Song C,et al.Astilbin ameliorates pulmonary fibrosis via blockade of Hedgehog signaling pathway[J].Pulm Pharmacol Ther,2018,doi:10.1016/j.pupt.2018.03.006.
[14]赵鑫,苟小军,陈龙,等.花旗松素预防CCl4致大鼠急性肝损伤的代谢组学研究[J].上海中医药杂志,2017,51(8):82-86.
[15]Guo X,Ding X.Dioscin suppresses the viability of ovarian cancer cells by regulating the VEGFR2 and PI3K/AKT/MAPK signaling pathways[J].Oncol Lett,2018,15(6):9537-9542.
[16]Ruiz-Miyazawa K W,Staurengo-Ferrari L,Mizokami SS,et al.Quercetin inhibits gout arthritis in mice:Induction of an opioid-dependent regulation of inflammasome[J].Inflammopharmacology,2017,doi:10.1007/s10787-017-0356-x.
[17]Kong Q,Zhang S,Liang C,et al.LncRNA XISTfunctions as a molecular sponge of miR-194-5p to regulate MAPK1 expression in hepatocellular carcinoma cell[J].J Cell Biochem,2018,119(6):4458-4468.
[18]Tian B,Widen S G,Yang J,et al.The NFκB subunit RELA is a master transcriptional regulator of the committed epithelial-mesenchymal transition in airway epithelial cells[J].J Biol Chem,2018,293(42):16528-16545.