不同活力花粉与其内含物及水分含量的变化及相关性研究
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摘要
【目的】研究花粉内含物的变化与花粉活力之间的关系。【方法】在4℃下贮藏砀山梨花粉,离体萌发法测定花粉活力,近红外光谱仪测定花粉的蛋白质、脂肪、硫甙、水分含量。【结果】随着花粉活力的下降,花粉中总蛋白质含量的增加,总脂肪含量呈锯齿状,硫甙含量呈锯齿状,含水量呈上升趋势。花粉活力与脂肪、硫甙、含水量与蛋白质之间有相互关系,花粉活力与脂肪r为0. 623**,花粉活力与硫甙r为0. 813**;花粉活力与含水量r为-0. 886**,花粉活力与蛋白质r为-0. 807**。【结论】脂肪、硫甙与花粉活力正相关,含水量、蛋白质与花粉活力呈负相关,花粉储藏应干燥以降低花粉呼吸代谢。
【Objective】We aim to explore the relationship between pollen inclusion and pollen viability.【Method】The pollen viability of Dangshan pear,which was stored at 4 degree centigrade conditions for for 0 days,7 days,27 days,43 days and 65 days,was determined by means of in vitro pollen germination method.And the content of the protein fatty glucosinolins water in pollen was measured by near infrared spectrometer.【Result】The results showed that: the protein content increased,the total fat content and glucosinolates were serrated,along with the pollen vitality decreased. The water content was increased with the increase of storage time and without desiccant. The studies have shown that pollen vitality are associated with fatty,glucosinolate,water content and protein. They correlation were 0. 623,0. 813,-0. 886,-0. 807,respectively. 【Conclusion】Fat and glucosinolates are positively related to pollen vitality,and water content and protein are negatively correlated with pollen viability,and pollen respiration is reduced by drying in long term pollen storage. It has positive correlation that the pollen viability with fatty,glucosinolate. The pollen viability has negative correlations with water content,protein. Therefore,pollen are dehydrated to conserve them for depressed respiratory action.
【Objective】We aim to explore the relationship between pollen inclusion and pollen viability.【Method】The pollen viability of Dangshan pear,which was stored at 4 degree centigrade conditions for for 0 days,7 days,27 days,43 days and 65 days,was determined by means of in vitro pollen germination method.And the content of the protein fatty glucosinolins water in pollen was measured by near infrared spectrometer.【Result】The results showed that: the protein content increased,the total fat content and glucosinolates were serrated,along with the pollen vitality decreased. The water content was increased with the increase of storage time and without desiccant. The studies have shown that pollen vitality are associated with fatty,glucosinolate,water content and protein. They correlation were 0. 623,0. 813,-0. 886,-0. 807,respectively. 【Conclusion】Fat and glucosinolates are positively related to pollen vitality,and water content and protein are negatively correlated with pollen viability,and pollen respiration is reduced by drying in long term pollen storage. It has positive correlation that the pollen viability with fatty,glucosinolate. The pollen viability has negative correlations with water content,protein. Therefore,pollen are dehydrated to conserve them for depressed respiratory action.
引文
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[9]Sharafi Y,Hajilou J,Mohammadi S A,et al.(2010). Pollen-pistil compatibility relationships in some Iranian almond(Prunus dulcis,Batch)genotypes as revealed by PCR analysis.African Journal of Biotechnology,9(23):3,338-3,341.
[10]Batos,Z. B,Nikolic,B. M.(2013). Variability of in vitro germination of Picea omorika pollen.[J]. Dendrobiology,69:13-19.
[2]徐叶挺,龚鹏,杨波,等.不同贮藏条件下引进扁桃花粉活力变化[J].新疆农业科学,2011,48(1):60-63.Xu Ye-ting,Gong Peng,Yang Bo,et al.(2011). Change of vitality of introduced almond pollen under different storage conditions[J]. Xinjiang Agricultural Sciences,48(1):60-63.
[3]卜路霞,李建颖,石军,等.天然硫甙化合物的结构、降解及其生物活性[J].天津农学院学报,2010,17(3):38-41.Bu Lu-xia,Li Jian-ying,Shi Jun,et al. Structure,degradation and bioactivity of natural glucosinolates[J]. Journal of Tianjin Agricultural University,17(3):38-41.
[4]吴正可,刘国华,蔡辉益,等.硫甙脱毒菌株的筛选及其发酵菜籽粕的效果[J].动物营养学报,2018,30(1):313-320.Wu Zheng-ke,Liu Guo-hua,Cai Hui-yi,et al.(2018).Screen of glucosinolate detox strain and its effects on fermentation of rapeseed meal[J]. Chinese Journal of Animal Nutrition,30(1):313-320.
[5]张洪伟,段一凡,李稚,等.不同贮藏方法对桂花花粉活力影响的研究[J].南京林业大学学报(自然科学版),2014,38(S1):7-12.Zhang Hong-wei,Duan Yi-fan,Li Zhi,et al.(2014).Study on effects of different storage methods on pollen vitality of Osmanthus fragrans[J]. Journal of Nanjiang Forestry University(Natural Sciences Edition),38(S1):7-12.
[6]王小敏,吴文龙,李维林,等.黑莓花粉含水量与贮藏特性的研究[J].江西农业学报,2013,25(5):35-37.Wang Xiao-min, Wu Wen-long, Li Wei-lin, et al.(2013). Study on moisture content of blackberry pollen and its storage characteristics[J]. Acta Agriculturae Jiangxi,25(5):35-37.
[7]杨晓宇,杨少玲,杨华.花粉资源利用研究进展[J].特产研究,2003,25(4):52-56.Yang Xiao-yu,Yang Shao-ling,Yang Hua.(2003). Progress in study on utilization of pollen resources[J]. Special Wild Economic Animal and Plant Research,25(4):52-56.
[8]李英华,胡福良,朱威,等.我国花粉化学成分的研究进展[J].养蜂科技,2005,(4):7-16.Li Ying-hua,Hu Fu-liang,Zhu Wei,et al.(2005). The recent advances in pollen components in china[J]. Apicultural Science and Technology,(4):7-16.
[9]Sharafi Y,Hajilou J,Mohammadi S A,et al.(2010). Pollen-pistil compatibility relationships in some Iranian almond(Prunus dulcis,Batch)genotypes as revealed by PCR analysis.African Journal of Biotechnology,9(23):3,338-3,341.
[10]Batos,Z. B,Nikolic,B. M.(2013). Variability of in vitro germination of Picea omorika pollen.[J]. Dendrobiology,69:13-19.